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Citations for Becton, Dickinson and Company :
1551 - 1600 of 6939 citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... the pLCOs in suspension were centrifuged at 500 × g for 5 minutes at 4 °C and then resuspended in cold growth factor-reduced Matrigel (BD Biosciences). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were incubated overnight at 4°C in the dark and sample analysis performed the following day using an LSR FortessaTM X-20 device (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... according to manufacturer instructions and stored at 4°C protected from light before acquisition on a FACSCalibur cytometer equipped with CellQuest Pro software (BD Biosciences) within 1 week of fixation ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained with the corresponding FACS panels (Extended Data Table 5) at 4 °C for 30 minutes and sorted using BD Aria III (BD Biosciences) or MoFlo Astrios EQ (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... The tube was then stored in the dark at 4°C for up to 2 hours prior to acquisition on a LSRII or FACSLyric analyzer (BD Biosciences).
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bioRxiv - Molecular Biology 2023Quote: ... the cells were fixed in 4% PFA for 15 minutes at room temperature and permeabilised in Perm III buffer (BD Bioscience) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained with Fixable Viability Dye (20 min, 4°C, Fixable LiveDead, eBioscience) and then with a mix containing a brilliant stain buffer (BD Biosciences) and the surface markers for CD4+ T cells detection (CD3 and CD4 ...
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bioRxiv - Bioengineering 2024Quote: ... washed and stored at 4 °C for analysis the next day on a on a flow cytometry instrument (LSR Fortessa, BD Biosciences). Flow cytometry buffer was used to prepare Fc block and antibody solutions ...
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bioRxiv - Immunology 2024Quote: ... The samples were then stained with the fluorophore-conjugated antibodies or isotype controls in a flow cytometry buffer for 30 minutes at 4 °C in the dark and analyzed with the FACS symphony A1 (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... the cells were fixed with 2% (w/v) paraformaldehyde and stored at 4°C before analysis with FACS Celesta Cell Analyzer (BD Biosciences). Data were analyzed using FlowJo software version X.0.7 (Tree Star ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescence intensity of Mag-fluo-4-loaded cells was measured using a BD Accuri C6 flow cytometer (BD Biosciences, CA, USA) with an FL1 detector for green fluorescence and logarithmic amplification ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... and stained for 30 min at 4 °C with the following antibody mixture: PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Fixed cells were stained for 30 min at 4° C with PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with a mouse pan α-synuclein primary antibody (Syn1, BD Biosciences, NJ, USA; #610786) diluted 1:5,000 in blocking solution ...
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bioRxiv - Genomics 2024Quote: ... The cells were then washed twice with FACS buffer and then analyzed for DAPI intentsity in LSR-Fortessa 4-9 HTS bench top flow cytometer (BD Biosciences). DAPI intensity for at least 10,000 individual cells were recorded for each sample.
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bioRxiv - Developmental Biology 2024Quote: ... hPSCs were seeded as single cells (2-4×105 cells/ml, see hPSC maintenance) on coated 12-well plates (BD Falcon). Cells were constantly maintained at 37°C in 5% CO2 with 95% air.
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bioRxiv - Physiology 2024Quote: ... antibodies (or isotypic controls) for 30 min at 4°C before analysis was run using a FACSCanto II flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... cell suspensions were incubated at 4°C or 37°C for 90 minutes and labelled with Annexin V-FITC or Annexin V-BV711 (BD Biosciences) in line with the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... A human EC model of obesity and diabetes was established by growing HUVECs to confluency on gelatin-coated 24-well TC plates or glass chamber slides (4-well or 8-well; BD Falcon) in EGM-2 and thereafter changing to growth factor-depleted (GFD ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cells were incubated for 4 h and washed twice with PBS for analysis of fluorescein positive cells via flow cytometer (BD FACSCantoTM II, BD Biosciences). To further investigate the internalization mechanism of PoLixNano ...
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bioRxiv - Cell Biology 2024Quote: ... iECs were dissociated with TrypLE Express and fixed with 4% paraformaldehyde with 0.2% Triton X-100 and then the cells were filtered with 70 μm cell strainers (BD Falcon, 38030) in 1% BSA-PBS ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed and fixed/permeabilized for 20 min at 4°C using the Cytofix/Cytoperm Fixation/Permeabilization Kit (BD Biosciences). Cells were washed with 1X PermBuffer (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... surface stained cells were fixed and permeabilized (20 min at 4°C) using Fixation/Permeabilization Solution before washes in Perm/Wash buffer (both BD Biosciences). Cells were then stained with intracellular Abs as above except in Perm/Wash buffer ...
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bioRxiv - Immunology 2024Quote: ... #DY413 and #DY404) for IL-4 and IL-13 and BD™ Cytometric Bead Array (CBA) Mouse Inflammation Kit (BD, #552364) for IL-2 ...
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bioRxiv - Microbiology 2024Quote: ... plates were washed 4 times with PBST and filled each well with 100 µl 1000 times diluted HRP-conjugated streptavidin (BD, 554066) in 0.5% skim milk PBST buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Antibody staining was performed at 4 °C for 30 min and the stained cells were analyzed on an LSRFortessa cell analyzer (BD Biosciences). FACS sorting routinely yielded cell purity levels of over 90 % ...
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bioRxiv - Bioengineering 2024Quote: ... the frozen aliquot was thawed in a 37°C water bath for 4 min before being diluted in 35 mL stain buffer (BD, 554656) and pelleted at 300g for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 4:1 CTL:cancer cell admixtures were incubated for 4 h at 37°C in the presence of BD GolgiPlug protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with the specific primary antibodies (1:300 CD24-PerCP-Cy5.5, BD 562360; 1:100 CD31-BUV395, BD 740239; 1:200 CD45-BUV395, BD 565967 ...
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bioRxiv - Neuroscience 2020Quote: ... CD8a APC-R700 (1:150 or 1:200, clone 53-6.7), CD69 BV421 (1:100, clone H1.2F3), CD25 BB515 (1:150, clone PC61) (BD Biosciences); CD4 FITC (1:500 ...
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bioRxiv - Immunology 2022Quote: ... and F15 NOD Nfκb1 heterozygote were suspended in biotin-free RPMI containing 0.1% azide and 3% FCS and surface stained in 96-well plates with the 10–3.6 PE (anti-I-Ag7) (BD PharMingen, Franklin Lakes, NJ, USA), which is class II major histocompatibility complex (MHC ...
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bioRxiv - Cancer Biology 2024Quote: ... The phosphorylation-SMAD2/3 ability of CAR T cells was detected using PE Mouse anti-Smad2/Smad3 antibodies (clone O72-670, BD Biosciences, Franklin Lakes, NJ) following the staining protocols ...
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bioRxiv - Bioengineering 2023Quote: ... pneumoniae serotype 3 (Sp3) (ATCC; Manassas, VA) was cultured on plates of BBL Trypticase Soy Agar with 5% sheep blood (BD Trypticase Soy Agar II) (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... analysis of sub-G0/G1 DNA content as previously described (23) and by measurement of active caspase-3 by flow cytometry using a commercial kit (BD Biosciences, Franklin Lakes, NJ).
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bioRxiv - Cancer Biology 2021Quote: ... BD Biosciences], 1:150 anti-CD226/DNAM-1-BV510 [clone DX11, BD Biosciences], 1:400 anti-CD3-AF700 [clone UCHT1, BD Biosciences] ...
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bioRxiv - Immunology 2021Quote: ... no stimulation and a peptide pool spanning the spike protein at a concentration of 1 μg ml−1 of each peptide in the presence of 1 μg ml−1 of anti-CD28 (clone CD28.2, BD Biosciences) and anti-CD49d (clone 9F10 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse primary at 1/100, Dako; CldU: rat primary at 1/1,000, Accurate Chemical; IdU: mouse primary at 1/1,000, BD Biosciences) and with a rabbit anti GFP (Millipore ...
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bioRxiv - Immunology 2021Quote: ... ratio of 10:1 (PBMC) or 1:1 (purified NK cells) in the presence of GolgiStop (0.7 μl/ml, BD Biosciences), Brefeldin-A (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... were washed with PBS and incubated with pMHC and additional antibodies (CD3-BV421, 1:20, BD; CD8-BV650, 1:100, BD; CD4-V500, 1:50, BD; CD137-APC ...
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bioRxiv - Immunology 2024Quote: ... 1:50, BD; CD137-APC, 1:25, BD; CD279-APC-Cy7, 1:50, ITK; and CD366-PE-Cy7, 1:10, BD). Events were acquired with FACS Celesta and analyzed using FlowJo and R packages ggplot2 and ComplexHeatmap94,95.
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bioRxiv - Physiology 2023Quote: ... Samples were next stained with surface marker antibodies (CD45-AF700 1:500 103128 Biolegend, CD31-Pacific Blue 1:500 102421 Biolegend, TER119-BUV496 1:500 741079 BD, PDGFRα-APC 1:500 135907 Biolegend ...
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bioRxiv - Immunology 2023Quote: ... 1:100) from eBioscience, and CD45 (564279, 1:200), IL-10 (566295, 1:100) and TCRγδ (563532, 1:100) from BD Bioscience.
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bioRxiv - Immunology 2023Quote: ... 1:400), B220 (RA3-6B2, 1:400), IgM (R6-60.2, 1:400) and CD43 (S7, 1:400) (all from BD Biosciences). Antibodies were directly coupled to fluorescein isothiocyanate ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were successively incubated with primary antibodies diluted in blocking buffer (mouse anti-G3BP1, 1:4000, BD Biosciences, rabbit anti-GFP, 1:1000, ThermoFisher, mouse anti-PKR, 1:1000, BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Cat. 562504, 1:200), Alexa Fluor 647 anti-TCF-1 (clone S33-966, Cat. 566693, 1:300) were from BD Pharmingen ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with polyclonal anti-CD3 (1 μg ml−1, BD Biosciences) in U-bottom 96-well plates ...
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bioRxiv - Bioengineering 2020Quote: ... Anti-CD31 (1:50, ab28364) and Ki-67 (1:100, BD 610968) were maintained at 4°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were resuspended in PBS/Matrigel mixture (1:1 ratio, BD Biosciences) to a final concentration of 4x 106 cells/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... CD49f PE-Cy5 (1:50) and FLT3 CD135 biotin (1:50, BD). Cells were washed and the following antibody was used for the second round ...