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Citations for Becton, Dickinson and Company :
1151 - 1200 of 1408 citations for 2 Pyridinesulfonic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... were subcutaneously inoculated with 1 × 106 LoVo or 2 × 106 LS180 cells in 1:1 mixture of PBS and matrigel (BD Biosciences) into lower right flank ...
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bioRxiv - Developmental Biology 2020Quote: ... The cells were washed 2 times in FCM Blocking Buffer then analyzed on a BD Accuri C6 Flow Cytometer (BD Biosciences).
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bioRxiv - Microbiology 2020Quote: ... 2 µg/ml lysostaphin, 2 mM CaCl2, 10 µl/ml annexin V-APC [BD Biosciences] and 10 µl/ml 7AAD [BD Biosciences]). After 10 minutes incubation in the dark cells were immediately analyzed by flow cytometry using a FACS Aria III (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed with PBS in the presence of 2 % BSA and incubated with anti-IFN-γ-PE-CY7 (BD Biosciences), anti-IL-2-APC (BD Biosciences) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were washed twice with FACS buffer and strained through a 100 μm filter before sorting CD11b+ microglia and ACSA-2+ astrocytes on a FACS Aria III (BD Biosciences) with a 100 μm nozzle ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then washed once in a large volume of PBS and post-fixated in 2% PFA in PBS before analysis on an LSRII flow cytometer (BD Biosciences). Data was analyzed using Flowing software (version 2.5 ...
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bioRxiv - Bioengineering 2021Quote: ... Binding of RBD to hACE-2 on the cell surface was assessed via the mean fluorescent intensity measured by flow cytometry using BD LSRFortessa (BD Biosciences).
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bioRxiv - Neuroscience 2021Quote: ... P0-P1 mouse pups were anesthetized in ice and then eyes were pierced at the edge of corneal by 30G × 1/2 needle (BD, 305106) under stereomicroscope ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated with drugs or DMSO 5 days after infection and the fraction of TUBB-expressing cells was measured 3 days after treatment and then every 2 days as the fraction of mCherry-positive cells by flow cytometry on an LSR-II flow cytometer (BD Biosciences). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed 3 times with PBS with Ca2+ and Mg2+ containing 2% FBS and mean fluorescence intensity per cell was measured by LSRII (BD Biosciences) flow cytometer the same day of the assay.
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bioRxiv - Developmental Biology 2022Quote: ... 2% FBS in Ca2+/Mg2+-free PBS) and DAPI negative cells were selected by cell sorting using FACSAria Fusion (BD Biosciences). Cell viability was above 70% for each sample ...
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bioRxiv - Immunology 2022Quote: ... 0.75 × 105 naïve T cells were cultured for one or two days with plate-bound anti-CD3ε (2 µg/ml, clone 145-2C11; BD Biosciences) in differentiation medium consisting of RPMI1640 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: Igλ-enriched B1-8 cells were cultured for 2-3 days at 4 *105 cells/well in a 12-well tissue culture plate (BD Falcon) and then washed ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Tissues were incubated at 4°C overnight with the following primary antibodies: monoclonal mouse anti-carboxyl-terminal binding protein 2 (CtBP2) IgG1 at 1:200 (612044; BD Biosciences) counterstained with goat anti-Mouse IgG1 conjugated with Alexa Fluor 568 (#A-21124) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were stored in DPBS with 2% FBS overnight at 4°C before data acquisition on a LSR6Fortessa analyser (BD Biosciences). Compensation was carried out using single stain control UltraComp eBeads (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed once with FACS buffer and fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). In some experiments ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining of IL-2 followed the same protocol as that for CTLA-4 but included the addition of 1 μg/mL GolgiPlug (BD Biosciences) to the culture media 12 hours prior to sample preparation ...
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bioRxiv - Developmental Biology 2020Quote: ... AAV9-mediated Snhg1 or NC were delivered by intracardiac injection into the left ventricle in 7-day-old mice at a dose of 2 × 1010 viral genome particles per animal using an insulin syringe with a 30-gauge needle (BD Biosciences). And the adult mice received the injection at a dose of 1 × 1011 viral genome particles per animal ...
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bioRxiv - Immunology 2020Quote: ... the following antibodies and viability dye were incubated with PBMCs at RT for 20 min: anti-CD3 BUV395 (clone SP34-2; BD Biosciences), anti-CD4 BUV495 (clone SK3 ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were processed as previously described47 and 1-2 million cells were injected into cleared mammary fat pads in 10-20 microliters of commercial Matrigel (BD Biosciences). For ER+ tumors ...
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bioRxiv - Microbiology 2021Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Microbiology 2021Quote: Mouse spleens were collected in ice-cold storage solution (PBS 2% FBS) and single-cell suspensions prepared using cell strainers (BD-Falcon). Cells were washed upon red blood cells lysis (150 mM NH4Cl ...
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bioRxiv - Immunology 2021Quote: ... single MR1-5-OP-RU-tetramer+TRAV1-2+ PBMCs from healthy donors and spleen tissues were sorted using a FACSAria (BD Biosciences) into 96-well plates ...
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bioRxiv - Immunology 2020Quote: ... PBMC (2 × 106 cells/well) were stimulated with anti-CD28 (1 μg/ml) and anti-CD49d (1 μg/ml) (FastImmune, BD Biosciences) and 1e5 M ...
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bioRxiv - Immunology 2020Quote: ... then washed with 2% FCS - PBS and stained with Mouse anti-IRF-7-PE (pS477/pS479, clone K47-671; BD Biosciences) or Mouse anti-NF-κB p65-Alexa Fluor 647 (pS529 ...
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bioRxiv - Physiology 2020Quote: ... 1 ng/mL FGF2 and 10 mg/mL gentamycin) and plated on dishes previously coated with 2% Matrigel™ (BD biosciences).
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bioRxiv - Cell Biology 2021Quote: ... macrophages were incubated with 5 μM 2’, 7’ dichlorofluorescein diacetate (DCFDA, Invitrogen at 37°C for 30 min, followed by acquisition using FACS Canto I (BD Sciences) within 30 minutes of staining ...
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bioRxiv - Genomics 2020Quote: ... Cells were then incubated with 5ul human Fc Block with 95ul 2% FBS in PBS at 4°C for 15 mins before staining with SCMK and AbSeq antibodies (BD Biosciences) at 4°C for 60 min ...
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bioRxiv - Neuroscience 2021Quote: ... The dissected tissue was mechanically triturated by a fire-polished glass pipette and plated onto 24-well plates coated with 2% laminin (BD Biosciences) and 1% poly-D-lysine (Sigma Aldrich) ...
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bioRxiv - Pathology 2021Quote: Cells were fixed in 2% PFA for 15 minutes at room temperature and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight as previously described 36 ...
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bioRxiv - Immunology 2020Quote: ... CD95, CD278) or intracellular proteins (BCL-2, BCL-6, CD152) and cells were acquired on an LSR II analyser (BD Pharmingen), followed by flow cytometric analysis using the FlowJo Software (FlowJo LLC ...
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bioRxiv - Microbiology 2020Quote: ... Footpads were digested with 4 mg ml−1 collagenase D for 2 h at 37°C before dissociation through 70 μm cell strainers (BD Biosciences). Homogenates were resuspended in HOMEM supplemented with 20% FCS and serial dilutions (2-fold ...
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bioRxiv - Biophysics 2020Quote: ... and a strain with a known binding site and repressor copy number (R) - were inoculated into individual tubes with 2 mL of Lysogeny Broth (LB Miller Powder, BD Medical) with 20 µg/mL of chloramphenicol and 30 µg/mL of kanamycin ...
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bioRxiv - Immunology 2020Quote: ... the cells were diluted in 2% exo-free FBS/PBS and analyzed on a BD-LSR II flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2020Quote: Cells were washed in PBS and resuspended in PBS containing 2% fetal calf serum and 2 mM EDTA and stained with the extracellular mix using anti-human CD14-BV421 antibody (clone M5E2, BD Bioscience) and anti-human CD36-BUV605 antibody (clone CD38 ...
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bioRxiv - Immunology 2021Quote: ... as a negative control in the presence of αCD28/αCD49d co-Stim antibodies (1 μg ml−1) GolgiStop (containing Monensin, 2 μmol/L), GolgiPlug (containing brefeldin A, 10 μg ml−1) (BD Biosciences) and anti-CD107α BV421 antibody (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed twice with PBS containing 2% FBS and were blocked with CD16/32 Fc block (Cat. 553142, BD Bioscience) before the analysis ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibody cocktail (Suppl. Table 2) for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Up to 5×106 cells were washed in cold FACS wash (PBS supplemented with 2% FCS and 2mM EDTA) prior to being stained with a surface receptor antibody cocktail containing FcBlock (BD, 2.4G2) and LIVE/DEAD fixable blue dead cell stain (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... The 24-color extracellular staining master mix (Extended Data Table 2) was prepared 2X in BD Horizon™ Brilliant Stain Buffer to prevent staining artifacts from BD Horizon Brilliant dye interactions and added 1:1 to cells ...
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bioRxiv - Cancer Biology 2020Quote: Cells either from culture or from transwell assay were washed in PBS and incubated in 2% FBS in PBS with anti-CCR7-FITC (1:25, #561271, BD Biosciences) and anti-ROR1-APC (1:25 ...
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bioRxiv - Pathology 2023Quote: ... Placentas were perfused for 3 hours and 1 ml (T4 transfer experiments) or 2 ml (TRIAC transfer experiments) samples were collected from the reservoirs into blood collection tubes (BD vacutainer) at the time points indicated in the figures ...
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bioRxiv - Immunology 2022Quote: ... The cells were fixed in 2% paraformaldehyde and GFP expression measured and quantitated using a FACS Canto II flow cytometer and FlowJo software (BD Biosciences). IC50 values were calculated using Prism software (GraphPad/Dotmatics).
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bioRxiv - Immunology 2023Quote: ... cells were fixed for a minimum of 20 minutes with 2% paraformaldehyde and acquired immediately using a FACS Symphony A3 (BD Biosciences). Where intracellular staining was performed ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted by their distinct proliferation peaks (peak 0, peak 1 and peak 2) on FACS-Aria and Influx Cell Sorters (BD Biosciences). Cells were kept on ice and after washing with cold PBS were immediately resuspended in Trizol for RNA extraction ...
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bioRxiv - Immunology 2022Quote: FACS-sorted Tregs were plated at 50,000 cells/well in round bottom 96 well plates pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) and soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... T cells isolated from lymph nodes or tumors were mixed with 3×106 congenically marked B6 splenocytes and incubated with 1 μg/mL of OVA peptide and/or 2 μg/mL of GP peptide for 4-5h at 37°C in the presence of GolgiPlug (BD Biosciences). After staining for cell surface molecules ...
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bioRxiv - Cancer Biology 2023Quote: ... were stained with the specified antibodies on ice for 30 min in D-PBS supplemented with 2% FBS in the dark and analyzed using a Fortessa or Symphony A3 (BD Biosciences). Data was analyzed using FlowJo10 (Tree Star Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed with PBS three times and resuspended in FACS buffer (PBS with 2%FBS) for analysis on BD Biosciences LSR Fortessa using a 488-nm laser and 530-nm/30 filter (BD Biosciences). Data analysis was performed with FCS Express 7 (De Novo Software) ...
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bioRxiv - Developmental Biology 2023Quote: ... resuspended in 300 μl of 2% FBS in PBS with 1000 ng/μl DAPI and processed by FACS on a Melody Cell Sorter (BD Biosciences) using the BD FACSChorus software ...