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Citations for Becton, Dickinson and Company :
1001 - 1050 of 1408 citations for 2 Pyridinesulfonic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... for 2 h and 4 h respectively at 37°C in 5% CO2 and exposed to brefeldin A (BD Biosciences) as described in previous studies ...
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bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured in T cell media supplemented with soluble anti-mouse CD28 (2 μg/ml, Clone: 37.51, BD Pharmingen) and 30 U/ml rhIL-2 (Roche ...
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bioRxiv - Bioengineering 2021Quote: ... at 2×106 cells/ml in wells coated with anti-mouse CD3e (1 μg/ml, Clone: 145-2C11, BD Pharmingen).
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bioRxiv - Bioengineering 2020Quote: ... The concentrations of cytokines IL-2 and IFN-γ were quantified using the corresponding ELISA kits (BD, 555190 and 555142).
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibodies diluted in PBS containing 2% NHS overnight (1:500 rabbit anti-Iba1, Abcam, ab178846; 1:200 mouse anti-Map2, BD Pharmagen ...
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bioRxiv - Immunology 2020Quote: ... 2 x 106 cells were cultured in 200μL final volume in 5mL polypropylene tubes (BD Biosciences, San Diego, CA, U.S.A) in the presence of anti-CD28 (1μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... xenograft models were generated by injecting SKOV3 cells [2 × 106 cells mixed 1:1 (v/v) with Matrigel (BD Biosciences)] subcutaneously ...
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bioRxiv - Systems Biology 2021Quote: ... The concentration of the cell suspension was adjusted to 2×106 cells ml-1 and subsequently aspirated into a 3 ml luer-lock syringe (BD).
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bioRxiv - Genomics 2021Quote: ... stimulated cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Alexa700; BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: 100 μl of washed and number-adjusted yeast suspension were injected into each caterpillar’s distal right proleg with a 30G1/2” needle (BD Microlance) and a 1 ml NORM-JECT syringe ...
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bioRxiv - Genomics 2020Quote: Primary human bronchial epithelial basal cells (Passage 1 or 2) were expanded on flasks coated with collagen I (BD Biocoat) in bronchial epithelial growth medium (BEGM) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were incubated on a cold block for 2-5 minutes before being plated in a 96 (12 x 8) grid on Omnitrays containing LB (BD, Franklin Lakes ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then washed with FC buffer (PBS supplemented with 2% HI-FBS and 0.05% NaN3) and resuspended in Fixation Buffer (BD Pharmingen)for 5 min at 4C° in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... and Western blots (WBs): monoclonal Abs (mAbs) anti-flotillin-2 (610383; WB: 1/1000) and anti-GM130 (610823; IF: 1/500) from BD Biosciences ...
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bioRxiv - Developmental Biology 2022Quote: ... E.coli strain Rosetta™ 2(DE3)pLysS bearing pET21d-FGF10 was grown in 2x YT medium (BD Biosciences, Cat#244020) with Carbenicillin (50µg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... Collagenase activity was quenched by dilution with FACS buffer (PBS, 0.5% FBS, 2 mM EDTA) and digestion products were filtered through 70 µm nylon filters (BD Biosciences). Cells were again washed with FACS buffer and immediately processed for immunostaining first by blocking Fc receptor binding with rat anti-mouse CD16/CD32 (eBioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... Flow cytometry profiles of the HSPC compartment (Extended Data Fig.2, Fig.9) were analysed using FlowJo software (version 10.1, BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... Pellets were resuspended in 2 ml of FACS buffer and fixed by adding 3.5 ml of fixation/permeabilization buffer (BD Biosciences) and rocking overnight at 4C ...
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bioRxiv - Pathology 2021Quote: ... Whole blood samples were then lysed and fixed in 2 ml of FACS Lysing Solution (BD Biosciences, San Jose, CA) for 10 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were first blocked for non-specific antigen binding (incubating for 20 min at RT with 2 µg/ml CD16/CD32 Rat anti-Mouse, unlabeled, Clone: 2.4G2, BD, 553142) then antibodies were added without washing off the blocking solution ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then stained with antibody (Supplementary Table. 2) and LSK cells were isolated through cell sorting using FACSAria (BD). Sorted LSK cells were cultured in StemSpan SFEM serum free media (stemcell technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Pre-coated coverslips were printed by stamping (0.2 mm spot diameter and 0.5 mm spot interspace) with a cell-attachment mixture of rat tail collagen (BD Biosciences), poly-D-lysine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... After staining for 45 min at 4°C cells were washed and taken up in 200 µl PBS+2%FBS and 1/1000 7AAD before being run on a LSR Fortessa (BD). Lineage out-put was analysed in FlowJo ...
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bioRxiv - Immunology 2022Quote: ... then fixed in 2% paraformaldehyde and stored at 4°C until acquisition on a FACSAria Fusion III flow cytometer (BD). Flow cytometry data was analysed with FlowJo version 9.9.6 (Tree Star).
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bioRxiv - Immunology 2022Quote: ... equal amounts of cell lysate premixes were pipetted to another flat-bottomed 96-well reaction plate containing 100 μl reaction buffer with 270 nM type 2 RNase H-specific substrate (BD) using a multi-channel pipette ...
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bioRxiv - Biochemistry 2022Quote: ... washed and resuspended in FACS buffer (2% FBS in 1X PBS) and filtered through filter-topped flow tubes (BD falcon) using a luer-lock syringe at 2×106 cells/ml ...
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bioRxiv - Microbiology 2020Quote: ... cells were resuspended and 2-43 binding to cell-surface S trimer was quantified on LSRII flow cytometer (BD Biosciences). The mean fluorescence intensity of APC in GFP-positive cells was analyzed using FlowJo and the relative binding of 2-43 to S trimer in the presence of competitors was calculated as the percentage of the mean fluorescence intensity compared to that of the competitor-free controls.
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bioRxiv - Immunology 2020Quote: ... Then cells were fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). For cell sorting ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer (PBS with 2% Heat-inactivated FCS) and fixed in 1x BD cellFIX (BD Biosciences). Samples were acquired on the LSRFortessa (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... the cells washed and stained with CD45.2-FITC (to identify leukocytes) and cell subset specific fluorochrome-conjugated mAb used in this study were obtained from BD Biosciences and Biolegend ...
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bioRxiv - Immunology 2020Quote: OT-I cells were expanded for 5 days in the presence of IL-2 after transduction and sorted on Thy-1.1 surface expression on a BD FACSAria Fusion cell sorter (BD Biosciences) directly into RLT Plus lysis buffer (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... at a concentration of 1 x 106 cells/mL with RPMI plus 2 μL/mL Leukocyte Activation Cocktail with GolgiPlug (BD) and incubated for 4 hours at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... cells were pelleted and then resuspended in 2 mL of 0.5% BSA in D-PBS for cytometer-based sorting (FACSAria IIIu cell sorter, BD Biosciences). An aliquot of 200 µL stained cells was saved as input for analysis ...
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bioRxiv - Immunology 2021Quote: ... followed by FcR blockage with mouse serum (2%) and FcR blocking anti-mouse CD16/32 antibodies (clone 2.4G2, BD Biosciences). Next ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed twice in PBS with 0.5% BSA and 2 mM EDTA and run on LSR II flow cytometer (BD Biosciences). Data were analyzed using FlowJo software ...
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bioRxiv - Bioengineering 2021Quote: ... All transformants were checked for the petite phenotype by growth on YEP-glycerol (1% w/v Bacto-yeast extract-BD Biosciences 212750, 2% w/v Bacto-peptone-BD Biosciences 211677 ...
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bioRxiv - Immunology 2021Quote: Cells resuspended in cold staining buffer (2% FCS in PBS) were blocked with mouse Fc Block (BD Biosciences, catalogue# 553141) for 10 minutes at 4°C and stained with a panel of antibodies (Supplementary Table 5) ...
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bioRxiv - Immunology 2021Quote: ... tibias and pelvis were harvested by crushing in PBS containing 2% of FBS and erythrocytes were lysed using a hypotonic buffer from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and IL-2 concentration were measured on non-diluted or 10-fold diluted supernatants using Human cytometric assay from BD according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... the invading cancer cells were fixed with 100% cold methanol and stained with 0.05 mg/mL 4,6-diamidino-2-phenylindole (DAPI; BD Biosciences, 564907). The invading cells in each chamber were counted under a fluorescence microscope ...
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bioRxiv - Cancer Biology 2020Quote: ... single cell suspensions were made (1.5 x 104 cells per ml) in the appropriate medium supplemented with 2% Growth Factor Reduced Matrigel (GFRM; BD Biosciences). 150μl of this mix was plated per well in a 96 well ImageLock plate (Essen Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... and the concentration of IL-2 in culture supernatants was determined by ELISA according to the manufacturer’s instructions (BD Biosciences).
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bioRxiv - Genetics 2021Quote: ... in 200 μl of 2% FBS/PBS (v:v) and calculated using DIVA before sorting on a FACSAria fusion III cytometer (BD Biosciences). In order to maintain the ratios of MuSCs ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5mM EDTA) containing the indicated fluorophor-conjugated antibodies (CD138-APC [281-2] and B220-PE [RA3-6B2] purchased from BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... were routinely grown at 37 °C under anaerobic conditions in a Bactron III-2 anaerobic chamber (Shellab, USA) in BHIS medium: 3.7% weight vol−1 brain heart infusion broth (BD, USA) supplemented with 0.5% weight vol−1 yeast extract (BD ...
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bioRxiv - Immunology 2020Quote: One hundred microliters of whole blood from each of the cynomolgus macaques was stained with combinations of fluorescence-conjugated monoclonal antibodies: anti-CD3 (clone SP34-2, Alexa700; BD), anti-CD4 (clone L200 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by fixation in 2% PFA in PBS before subjecting them to flow cytometry using the LSR-II (BD Biosciences). Data was analyzed by FlowJo v10 and graphed by Prism 8 (GraphPad).
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bioRxiv - Immunology 2020Quote: ... USA) for 2 hrs followed by the addition of Brefeldin A (10 µg/ml) (BD Biosciences, San Diego, CA, USA) after which the cells were further incubated for 16 hrs ...
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bioRxiv - Immunology 2020Quote: ... T cells were 2-way sorted from PBMC pre-gated on CD4+ CD14− viable lymphocytes using a FACS Aria (BD) and the antibodies listed in supplementary material procured from Biolegend or BD ...