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Citations for Becton, Dickinson and Company :
1051 - 1100 of 1110 citations for 7 BROMOMETHYL 4 CHLORO QUINAZOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Brains from paraformaldehyde-perfused 12 month-old mice were sectioned and 30 µm free-floating sections were incubated overnight at 4°C with primary antibodies (anti-αsyn, BD Transduction, 1:700), anti-DNAJC5 (Millipore ...
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bioRxiv - Immunology 2020Quote: ... Cells were then spun down at 500 × g for 5 min at 4°C and resuspended with the following antibody-conjugated fluorophores for 20 minutes at room temperature: anti-CD3-BV421 (BD, 1F4, 1:100), anti-CD4-PE-Cy7 (BD ...
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bioRxiv - Cell Biology 2019Quote: ... stably transduced mApple and eGFP EpH4 cells were sorted using a BD 4 laser FACSAria III cell sorter (BD Biosciences, San Jose, CA), treated for 24 hours with 1 μM Cisplatin or PBS vehicle ...
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bioRxiv - Systems Biology 2021Quote: ... HeLa and RAW 264.7 cells were washed by chilled PBS and sorted on the nanoPOTS chips (4 × 12, 1.2 mm diameter per well) using the Influx II cell sorter (BD Biosciences, San Jose, CA) as described previously 14 ...
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bioRxiv - Immunology 2020Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... were plated around the rim of the well of a 96-well plate in a 4:3 mixture of Matrigel and MammoCult medium (BD Bioscience CB-40324). The cell mixture was then incubated at 37°C with 5% CO2 for 15 min to solidify the gel ...
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bioRxiv - Immunology 2022Quote: ... mice were euthanized and tumors were quickly harvested and fixed for 14-16 hr at 4&C in BD Cytofix/Cytoperm (BD Bioscience, Cat #: 554722) diluted 1:4 in PBS ...
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bioRxiv - Immunology 2022Quote: ... In-between incubation steps, cells were washed (300 x g, 10 min, 4°C) with 40 mL of BD Cell Wash (BD Biosciences, Allschwil, Switzerland). In order to block Fc receptors ...
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bioRxiv - Microbiology 2022Quote: ... 98 mL of fresh MRS medium is then inoculated with 2 mL of the overnight culture and incubated at 37°C without shaking to an OD of 0.5-0.6 (ca. 4-5 hours) in a GasPak anaerobic jar (BD GasPak™ EZ container systems). Cells are harvested a first time by centrifugation at 4,000 g for 5 min ...
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bioRxiv - Systems Biology 2022Quote: Single cell suspensions were plated around the rim of the well in the 3:4 mixture of Mammocult and Matrigel (BD Bioscience CB-40324). We seed 5’000 cells/wells for mini-rings in white 96-well plates (Corning #3603 ...
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bioRxiv - Microbiology 2022Quote: ... Afterwards the samples were fixated and permeabilized for 20 min and 60 min at 4 °C in the dark using BD Cytofix/Cytoperm™ solution and BD Perm/Wash™ solution (BD Biosciences), respectively ...
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bioRxiv - Microbiology 2022Quote: ... the individual co-cultures were 10-fold serially diluted with 1X anaerobic phosphate buffer saline (PBS) and plated on Xylose Lysine Tergitol 4 (XLT4) agar (BD Difco, Houston, TX). The plates were incubated aerobically at 37°C for 24 hours followed by plating on XLT4 agar and colony forming units (CFU ...
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bioRxiv - Cancer Biology 2023Quote: ... overnight and then stimulated with 1 nM N4 in the presence of vehicle or Compound 182 (1 μM) for 4 h at 37°C in complete T cell media supplemented with BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were then stained with fluorophore-conjugated antibodies to cell surface CD8 and intracellular IFNγ ...
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bioRxiv - Immunology 2023Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The bottom and top parts of the membrane were then incubated overnight at 4°C with primary p53 antibody (2 µg/mL, 1:250) (Clone DO-1, BD Biosciences, Cat. 554293) and primary vinculin antibody (1:10,000 ...
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bioRxiv - Developmental Biology 2023Quote: A total of 4 ml of blood were withdrawn and transferred to BD Vacutainer™ Hemogard closure plastic K2-EDTA tubes (BD 367525, BD). Tubes were inverted 10 times to ensure blood and EDTA were well mixed and kept at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667, 1:100 in 1X BD Perm/wash solution) or anti-BST2 (Proteintech #13560-1-AP ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was washed with 3-4 ml washing buffer which was fresh PBSB 1% and then was filtered through a 70-µm cell strainer (BD Bioscience, NY, USA). Then using a long tube ...
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bioRxiv - Genomics 2023Quote: ... and half of the remaining chromatin was rotated overnight at 4°C with 4.5 µg of anti-p53 (BD Biosciences #554293, Lot #9046678). The other half of the remaining chromatin was also rotated overnight as a no-antibody control ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD ...
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bioRxiv - Immunology 2023Quote: ... Note these experiments were carried out using early access kits from BD Genomics before the implementation of commercially-available single-cell protein/RNA assays (e.g. Feature Barcoding, 10x Genomics; BD Abseq, BD Genomics, Supplemental Table 4), and researchers are recommended to use those newer solutions for any follow-up studies as the techniques and reagents have been refined ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were washed with PBS and stained for 20min at 4°C with anti-EphB2 APC conjugated antibody (BD Biosciences, Heidelberg, Germany). Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... and samples were incubated in the 9-well plates with 100 μL per plate overnight at 4°C: CtBP2 mouse IgG1 (BD Biosciences 612044; RRID:AB_399431), GluA2 mouse IgG2a (Millipore MAB397 ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Microbiology 2021Quote: ... and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution, BD Pharmingen, cat No. 559864). Cell nucleus was stained with DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 4% milk for 1 hour and incubated overnight at 4°C with shaking in the appropriate primary antibody against Nischarin (1:500, BD Biosciences, Cat No: 558262), GFP (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Cochlear whole mounts were immunostained overnight at 4°C with primary antibody against CTBP2 (mouse anti-CTBP2 at 1:200; BD Transduction Labs, Heidelberg, Germany). Secondary antibody (donkey anti mouse conjugated with Cy3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated overnight at 4°C in solution containing 1% BSA and primary antibodies: mouse F4/80 (T45-2342, BD Biosciences, New Jersey, USA) 1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated as described above and stained at 4°C (15 min) with fluorochrome-conjugated antibodies against surface markers of endothelial cells (CD31 (BD Biosciences, Franklin Lakes, USA) and myeloid cells (CD45 (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... surface stained, fixed with 4% formaldehyde solution (Tousimis, Rockville, MD) and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA). Antibodies used for flow cytometry included anti-human CD3 AF700 (clone UCHT1) ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C with a cocktail of mouse anti-human antibodies: CD19 Alexa 700 (HIB19, BD Biosciences, San Jose, CA), CD21 BV421 (B-ly4 ...
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bioRxiv - Immunology 2022Quote: ... the cells were fixed with 2% PFA and stored at 4°C until the samples were acquired on a LSRII cytometer (BD Biosciences, Mississauga, ON, Canada). Data analysis was performed using FlowJo v10.5.3 (Tree Star ...
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bioRxiv - Pathology 2019Quote: ... Samples were fixed with BD Biosciences stabilizing fixative and stored in the dark at 4°C until acquisition on a LSR Fortessa (BD Biosciences, San Jose, CA). Data were analyzed using FlowJo v 10.1r5 (FlowJo LLC ...
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bioRxiv - Cancer Biology 2019Quote: Glioma TMA sections (4 μm) were stained using a rat monoclonal primary antibody to the integrin β4 subunit (CD104; clone 439-9B; BD Pharmingen, San Jose, CA) at a concentration of 5μg/ml according to a previously described protocol (11) ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed and permeabilized for 30 min at 4 °C in the dark after extracelluar stainings using BD Cytofix/Cytoperm (Fixation and Permeabilization Solution, BD Biosciences, Cat: 51-2090KZ) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... For the induction of inflammatory responses PD fluid treated mice received intra-peritoneal injections of 400 μL 4% Brewer modified thioglycollate medium (BD Biosciences, San Jose, CA) or sterile saline as control ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were blocked in 5% BSA and probed overnight at 4°C with the following primary antibodies and dilutions: p16 (1:200, #554079 BD Biosciences, San Jose, CA), p53 (1:200 ...
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bioRxiv - Bioengineering 2022Quote: ... Membranes were washed 5x times with TBS-T and stained overnight at 4°C with gentle agitation with primary antibodies: mouse anti-rat CD63 (1:200 dilution, BD Biosciences, Mississauga, ON, Canada), rabbit anti-rat CD9 (1:500 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... Cells grown on 96 well plates were fixed and permeabilized for 20 mins at 4°C with Fixation/Permeabilization solution (BD Biosciences, cat. no. 554714). Cells were washed 3 times in PBS ...
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bioRxiv - Microbiology 2022Quote: ... oxysporum conidia were isolated after growth for 3-4 d in potato dextrose broth (PDB; Becton, Dickinson and Company, Le Pont de Claix, France) by filtering through two layers of Miracloth® (Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked by incubation in 5% non-fat milk powder in 0.1% Tween 20 in Tris buffered saline (TBS-T) and incubated overnight at 4°C with primary antibodies: ALIX (BD Biosciences, 611620, 1:1000), calnexin (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were fixed in 4% paraformaldehyde and permeabilized using a commercially available saponin-based permeabilization and blocking reagent (1x BD Perm/Wash buffer #554723). Cells were then stained using primary antibodies against Flag (Proteintech 20543-1-AP ...
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bioRxiv - Bioengineering 2024Quote: ... The stained cell suspensions were fixed in 4% paraformaldehyde and resuspended in PBS (1% BSA) before flow cytometry analysis using a FACSymphony™ A3 cell analyzer (BD Biosciences, NJ, U.S.A.).
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bioRxiv - Molecular Biology 2020Quote: ... The samples were then stained for 30min at 4°C with the following antibodies: monoclonal anti-human CD33 V450 (WM53; BD Biosciences, San Jose, CA, USA); HLA-ABC FITC (W6/32 ...
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bioRxiv - Immunology 2019Quote: ... Serum was collected by tail-tipping before LPS injection and 2 and 4 hours following injection for IL6 determination by ELISA (BD; OptEIATM Set Mouse IL-6), as per manufactures instructions.
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bioRxiv - Immunology 2021Quote: ... or PMA + ionomycin in the presence of brefeldin A for 4 h and stained using the Cytofix/Cytoperm Kit (BD Bioscience, Franklin Lakes, NJ, USA). Foxp3-positive cells and apoptotic cells were detected using the BD Foxp3 Staining Kit (BD Bioscience ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... Cells were then incubated for 1h at RT (or left at 4°C overnight) in the Iridium-intercalator solution in fixation and permeabilization buffer (BD Cytofix/Cytoperm™, BD Biosciences). Then cells were washed with 1x permeabilization buffer (BD Perm/Wash™ ...