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Citations for Becton, Dickinson and Company :
951 - 1000 of 1110 citations for 7 BROMOMETHYL 4 CHLORO QUINAZOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Flow cytometry evaluation was performed the following day using a 4-laser (405 nm, 488 nm, 561 nm, 640 nm) LSRFortessa analyzer (BD instruments). The following antibodies and reagents were used for flow cytometry experiments ...
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bioRxiv - Immunology 2022Quote: ... Cells were then resuspended in FACS buffer (1% BSA in D-PBS) and kept at 4°C in dark until analyzed with a customized BD LSR Fortessa flow cytometer (BD Biosciences). Compensation was performed using ArC and UltraComp compensation beads (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and cells fixed in 4% paraformaldehyde (PFA) were analyzed on a Stratedigm (S1000EXI) cytometer with post- processing using FlowJo software (BD Biosciences). For all other analyses ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: Lymph node single cell suspension of 2.5×106 cells was incubated for 20 min at 4°C with a purified rat IgG2b anti-mouse CD16/CD32 receptor antibody (BD biosciences). Cells were stained with fluorochrome-conjugated antibodies against CD9 ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood chimerism was determined in recipients by blood collection via cheek bleeds every 4 weeks for 16 weeks and cells were analyzed by flow cytometry using the LSRII (BD Biosciences). Long-term multi-lineage reconstitution (LTMR ...
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bioRxiv - Pathology 2023Quote: ... then incubated for 20 minutes at 4°C with a combination of the following mouse anti-human antibodies: AF488 anti-CD3 (clone UCHT1, BD Biosciences), PE anti-CD203c (clone NP4D6 ...
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bioRxiv - Immunology 2023Quote: ... T cells isolated from lymph nodes or tumors were mixed with 3×106 congenically marked B6 splenocytes and incubated with 1 μg/mL of OVA peptide and/or 2 μg/mL of GP peptide for 4-5h at 37°C in the presence of GolgiPlug (BD Biosciences). After staining for cell surface molecules ...
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bioRxiv - Immunology 2023Quote: ... for 20 mins at RT and then fixed samples were then resuspended in 100µL wash buffer prior to acquisition on the 4-laser BD® LSRII (BD Biosciences). Instrument setup and calibration were performed using EuroFlow standard operation procedures as described previously.43
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bioRxiv - Developmental Biology 2023Quote: MACS depleted non-hematopoietic cells were stained with the antibodies listed in Table S1 for 20 min at 4°C and analyzed on a spectral cytometer ID7000 (SONY) as described(4) or sorted with a BD FACS Aria III (BD Biosciences) according to the guidelines for the use of flow cytometry and cell sorting(5).
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bioRxiv - Developmental Biology 2023Quote: Hematopoietic cells were stained with the antibodies listed in Supp Table S1 for 20-30 min at 4°C and analyzed on a custom BD LSR Fortessa (BD Biosciences). For sorting ...
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bioRxiv - Genetics 2023Quote: ... and CD43-B cells were cultured at a density of 5×105 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). Cultured cells were maintained daily at a density of 0.5-1×106 cells per ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation was overnight at 4 degrees C with the following antibodies at 1:100 dilution in PBS + 3% BSA: CD24-FITC (clone ML5, BD Bioscience), CD271-APC (clone C40-1457 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated for 15 min at 4 °C with a purified mouse anti-rat CD32 FcγII (clone D34-485, Rat BD Fc Block) to block unspecific binding ...
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bioRxiv - Immunology 2023Quote: T cells were staining 30 min at 4°C with anti-human CD3-PE (Cyto Matin Gene, CMGCD3-P100) anti-CD3-PerCP (BD, 347344), anti-CD3-FITC (BD ...
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bioRxiv - Genomics 2023Quote: ... for 5 minutes and stained at a dilution of 1:50 with one of two panels of directly conjugated antibodies for 30 minutes at 4 °C: anti-human CD45 (BD 563792), CD204 (Biolegend 371906) ...
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bioRxiv - Immunology 2023Quote: 50 × 103 effector cells were cultured alone or together with 5 × 103 tumour cells for 4 h at a 10:1 ratio in the presence of anti-CD107a-PE mAb (clone: H4A3; BD Biosciences) and monensin (3 μM ...
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bioRxiv - Bioengineering 2023Quote: ... was grown overnight (37°C, 4% H2, 5% CO2, 91% N2, <10 ppm O2) in Chopped Meat Carbohydrate Broth (BD297307, BD, USA). Genomic DNA was extracted from B ...
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bioRxiv - Cell Biology 2023Quote: ... 10-μM bromodeoxyuridine (BrdU) solution was added 4 h before the end of the experiment [fluorescein isothiocyanate (FITC) BrdU Flow Kit (BD #559619)] according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... They were finally washed with 4 mL FACS buffer and re-suspended in 0.5 mL to be analyzed in a LSR Fortessa X20 (BD Biosciences, Europe). For live staining ...
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bioRxiv - Developmental Biology 2023Quote: A total of 4 ml of blood were withdrawn and transferred to BD Vacutainer™ Hemogard closure plastic K2-EDTA tubes (BD 367525, BD). Tubes were inverted 10 times to ensure blood and EDTA were well mixed and kept at room temperature (RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then fixed (eBioscience #00-8222-49) at 4 °C for 20 minutes and permeabilization (eBioscience #00-8333-56) before staining for intracellular IFNγ (BV650, BD #563854) and TNFα (BV421 ...
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bioRxiv - Microbiology 2023Quote: Stained cells were stored for up to 2 days at 4°C in the dark (TRAIL-R1) and flow cytometry was performed on a BD FACSCanto II (BD Biosciences). Raw data were exported to FlowJo v10.8.1 for analysis and plotting.
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then fixed in 2% formaldehyde and stored at 4°C until flow cytometry on an LSR II flow cytometer (BD Biosciences). Cell surface markers included CD3 (clone 17A2 ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
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bioRxiv - Immunology 2024Quote: ... surface antibodies were incubated with cells for 30 minutes at 4°C in hFACS buffer and Brilliant Stain Buffer (BD Biosciences). For intracellular staining ...
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bioRxiv - Neuroscience 2023Quote: ... Immunopurified Ngfrp75-positive mouse OECs were plated on PLL-coated 4-chamber polystrene-vessel culture slides (BD Falcon, San Jose, CA) or used for western blots ...
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bioRxiv - Immunology 2023Quote: ... The tube was then stored in the dark at 4°C for up to 2 hours prior to acquisition on a LSRII or FACSLyric analyzer (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... the cells were stained with the corresponding FACS panels (Extended Data Table 5) at 4 °C for 30 minutes and sorted using BD Aria III (BD Biosciences) or MoFlo Astrios EQ (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were fixed in 4% PFA for 15 minutes at room temperature and permeabilised in Perm III buffer (BD Bioscience) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were incubated overnight at 4°C in the dark and sample analysis performed the following day using an LSR FortessaTM X-20 device (BD Biosciences).
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bioRxiv - Bioengineering 2023Quote: ... The cell suspension was incubated with the following antibodies for 30 minutes at 4 °C: CD106-PE (VCAM-1, 555647, BD Biosciences), CD62E-FITC (E-Selectin ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... E8.5 embryos were fixed with 4% paraformaldehyde (PFA) in PBS at 4°C for 3 h and incubated with FITC-conjugated anti-EEA1 (1:100, BD Bioscience) or Cy3-labeled anti-mouse IgG (1:500 ...
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bioRxiv - Microbiology 2023Quote: Ferrets were sedated with 4% isoflurane and blood was collected via the jugular vein into a heparinized collection tube (BD 366643). Plasma was separated via centrifugation and stored at –80°C until use ...
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bioRxiv - Cancer Biology 2023Quote: ... the pLCOs in suspension were centrifuged at 500 × g for 5 minutes at 4 °C and then resuspended in cold growth factor-reduced Matrigel (BD Biosciences). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed two times with 150 µL PBS (centrifuged 5 min at 400 × g at 4 °C) and resuspended in 50 µL Fc-block (1:50, Purified Rat Anti-Mouse CD16/CD32, BD, #553142) diluted in FACS buffer (1x PBS/2% FBS/2mM ...
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bioRxiv - Immunology 2023Quote: ... Effector cells were incubated with K562 and Nalm6 variants for 4 hours at a ratio of 5:1 with addition of Brefeldin A (GolgiPlug, BD Biosciences). Next ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed with staining buffer, fixed, and permeabilized (20 min, 4°C, dark) using Fixation and Permeabilization Solution (BD Biosciences). Cells were then stained with S100A9 (Clone ...
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bioRxiv - Neuroscience 2023Quote: ... cell suspension was incubated with Fc block antibodies for 5 minutes at 4 °C and then labeled with anti-mouse Thy1.2 magnetic particles (BD IMag™) for 30 minutes at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: Both WT and CD13KO mice of both genders were intraperitoneally injected with 1 mL 4% Brewers Thioglycollate (TG) solution (BD; 211716). After 48 hours mice were then euthanized via CO2 and cervical dislocation and injected with 10 mL cold PBS into the peritoneal cavity to collect peritoneal macrophages ...
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bioRxiv - Immunology 2023Quote: ... according to manufacturer instructions and stored at 4°C protected from light before acquisition on a FACSCalibur cytometer equipped with CellQuest Pro software (BD Biosciences) within 1 week of fixation ...
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bioRxiv - Physiology 2024Quote: ... Small intestine lamina propria cells from male C57BL/6J mice were prepared and stained with surface molecules for 30 min at 4 followed by sorting on a FACS Aria III cell sorter (BD Biosciences). A gating strategy of live Lineage−CD127+KLRG1-c-Kit+ was used to sort the ILC3s.
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bioRxiv - Immunology 2024Quote: Live single CD45+TCRβ+CD4+IL10+ cells were sorted from isolated lamina propria cell suspension from three pooled Giardia-infected mice per sample (total of 4 samples) using a BD FACSAria Cell Sorter (BD Biosciences). Then ...
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bioRxiv - Cell Biology 2023Quote: ... 0.25% Triton PBS before incubation overnight at 4°C with the following antibodies: Rat monoclonal to mouse Sca1 (740450, BD Biosciences), Rabbit monoclonal to mouse SOX9 (ab185230 ...
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bioRxiv - Plant Biology 2023Quote: ... The flowers and the fixing solution were removed and replaced with 1 µg/mL DAPI (4′,6-diamidino-2-phenylindole, BD Biosciences) diluted in pollen isolation buffer before visualizing under a fluorescence microscope using a 420 nm filter (Zeiss LSM880 Inverted Confocal Microscope) ...
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bioRxiv - Immunology 2021Quote: ... Slides were treated for 10 minutes in H2O2 and after blocking in 10% goat serum in 0.1% Tween20 for 30 minutes and incubated overnight at 4°C with specific antibody diluted in PBS 0,1%Tween20: anti-mouse Ly6G (1A8, BD Pharmingen, cat 551459), or anti-mouse Ki67 (D3B5 ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at room temperature and incubated overnight at 4° C with primary antibodies against Syn1 (mouse, 1:1000, BD Biosciences, #610786), pSer129 (rabbit ...
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bioRxiv - Systems Biology 2019Quote: ... Membranes were incubated with primary antibody solutions (using 5% milk or BSA in TBST) overnight at 4°C at the respective dilutions: E-Cadherin (BD, #610181,1:5000), Vimentin (Abcam ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... Cells were then incubated for 1h at RT (or left at 4°C overnight) in the Iridium-intercalator solution in fixation and permeabilization buffer (BD Cytofix/Cytoperm™, BD Biosciences). Then cells were washed with 1x permeabilization buffer (BD Perm/Wash™ ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4 μL of the saturated culture were transferred in 60 uL PBS and the samples were stored at 4 °C for 1-2 days before being assayed by flow cytometry (BD FACSCanto II). Data were analyzed in FlowJo ...