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Citations for Becton, Dickinson and Company :
1051 - 1100 of 2969 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The cells were incubated with Fc block (eBiosciences 16-0161-81) for 5 min then incubated with antibodies (CD45-APC BD Biosciences 559864, and CD11b-BV421 BD Biosciences 562605) for 30 min at 4°C in incubation buffer (0.5% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were incubated with Fc block (eBiosciences 16-0161-81) for 5 min then incubated with antibodies (CD45-APC BD Biosciences 561018, and CD11b-BV421 BD Biosciences 562605) for 30 min at 4°C in FACS buffer (0.5% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with Fc block (eBiosciences 16-0161-81) for 5 min then incubated with antibodies for CD45-APC (BD Biosciences 561018) and CD11b-BV421 (BD Biosciences 562605 ...
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bioRxiv - Immunology 2021Quote: ... 106 cells were stimulated with 1µg/mL CMVpp65 overlapping peptides (JPT Peptide Technologies, Germany) in the presence of Brefeldin A (GolgiPlug (final concentration 5 µL/mL, BD Biosciences, USA) and Monensin (GolgiStop (final concentration 3.25 µl/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Then cells were blocked by PBS plus 5% BSA and later incubated with FITC Anti-Human CD24 (BD Biosciences, San Jose, CA), and PE Anti-Human CD44 (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... was plucked from each MOX plate and added to 5 mL of Brain Heart Infusion (BHI; BD Bacto, Franklin Lakes, New Jersey) broth and incubated at 25°C in a shaking water bath for 24 h ...
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bioRxiv - Cancer Biology 2020Quote: ... supernatant and attached cells were collected and stained with DAPI for 5 min before acquisition in BD FACS Canto II flow cytometer (BD Biosciences, USA). Results were analysed using the BD FACS DIVA (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... 5×104 cells were added to the top chamber of 24-well transwells (8 μm pore size membrane; BD Bioscience, Bedford, MA), and assay medium was added to the bottom chambers and incubated for 24 hours (10% FBS) ...
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bioRxiv - Microbiology 2022Quote: ... Samples were taken from each bottle in an anoxic chamber (95% N2 and 5% H2; Grass Lake, MI, USA) using a 23-gauge needle (BD PrecisionGlide™) and acid-washed 1 mL plastic syringe ...
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bioRxiv - Microbiology 2022Quote: ... from each bottle in replicate in an anoxic chamber (95% N2 and 5% H2; Coy Laboratory Products, Grass Lake, MI, USA) using a 23-gauge needle (BD PrecisionGlide™) and 1 mL plastic syringe ...
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bioRxiv - Neuroscience 2020Quote: ... centrifuged 500 x g for 5 min before red blood cells were lysed in 1X PharmLyse (BD Biosciences, San Jose, CA USA) for 8 min at room temperature (RT) ...
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bioRxiv - Immunology 2020Quote: ... Bacteria from cryo stock were resuspended in Brain Heart Infusion (BHI) medium and put onto blood agar plates (Columbia agar with 5 % sheep blood, BD, Cat: 4354005). Plates were incubated in a chamber with anaerobic conditions (83 % N2 ...
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bioRxiv - Immunology 2020Quote: ... Cellular depletion in spleens and intraepithelial lymphocyte compartments was monitored by flow cytometric detection with α-CD4 BV650 (clone RM4-5, BD Biosciences) or α-CD8β (clone H35-17.2 ...
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bioRxiv - Bioengineering 2020Quote: Seven days after electroporation a total of 5 × 106 T cells were collected and stained in 100 uL with fluorophore-conjugated anti-human antibodies for CD3 (BD Biosciences #564001), beta-2-microglobulin (BioLegend #316306 ...
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bioRxiv - Microbiology 2022Quote: ... jejuni on MH agars were resuspended in 5 ml of MH broth in a 14 ml round bottom tube (BD Falcon, USA) to an optical density at 600 nm (OD600 ...
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bioRxiv - Cancer Biology 2020Quote: ... 5×104 were added to the top chamber of 24-well Boyden chambers (8 μm pore size membrane; BD Bioscience, Bedford, MA), and medium was added to the bottom chambers and incubated for 24 hours (DMEM 10% FBS) ...
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bioRxiv - Microbiology 2020Quote: ... cells were restimulated for 5 hours with the same peptide pool in the presence of brefeldin A (BD Biosciences, San Diego, CA). Cells were then tested for IFN-γ ...
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bioRxiv - Microbiology 2021Quote: ... for in vitro experiments and LB agar (10 g/liter Bacto tryptone, 5 g/liter Bacto yeast extract, 10 g/liter sodium chloride, 1.2% agar; BD, San Jose, CA) supplemented with 750 μg/ml ampicillin (EMD Millipore ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody dilutions were prepared as follows in 5% NGS in 1X PBST: rat anti mouse CD11b clone M1-70 (BD Pharmingen; #553308) – 1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... The following antiboodies were used in combination for the depletion of lineage-positive cells: anti-CD4 (RM4-5, BD Biosciences, 1:100), -CD8 (53-6.72 ...
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bioRxiv - Neuroscience 2021Quote: ... and 5 mM EDTA in DPBS and kept cool before undergoing fluorescent activated cell sorting on a FACS Aria™ III (BD Biosciences). Dead cells were excluded from the sort and microglia were selected using CD11b/CD45 double staining and GFP as a marker of infection ...
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bioRxiv - Microbiology 2020Quote: RNA and virus injections of 5 and 10 μl were performed with insulin syringes (BD Micro Fine Plus, 1 ml, 30 G) into honey bee and bumble bee pupae respectively ...
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bioRxiv - Immunology 2020Quote: ... spleen cells were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug™, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 monoclonal antibody (mAb ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and centrifuged at 1000rpm for 5-10min and stained with APC-Cy™7 Rat Anti-Mouse CD3 (clone: 17A2, Cat # 560590, BD Biosciences), FITC Rat Anti-Mouse CD4 (Clone ...
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bioRxiv - Physiology 2020Quote: ... Microtubules were allowed to grow from SVP in DMEM-F12+10% FBS for 5 days before staining with an anti-CD31 antibody (BD Biosciences, 557355). Images of microtubule outgrow from SVP were acquired with a Leica SP5-SMD confocal microscope ...
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bioRxiv - Microbiology 2023Quote: BALF samples were centrifugated at 300 x g for 5 min and the pellets were resuspended in 500 μl Pharm Lyse Buffer (BD Biosciences, 555899) for 3 min at room temperature to lyse red blood cells ...
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bioRxiv - Microbiology 2023Quote: BALF samples were centrifugated at 300 x g for 5 min and incubated in 500 μl Pharm Lyse Buffer (BD Biosciences 555899) for 3 min at room temperature to lyse red blood cells ...
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bioRxiv - Microbiology 2023Quote: ... We then sheared the flagella by repeated passage through two 5-ml syringes connected by 22-inch-long tubing (ID 0.023”, BD INTRAMEDIC, BD Medical), about 30 times ...
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bioRxiv - Bioengineering 2023Quote: ... at 10 × 106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences, US) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was done by standard techniques following 5-hour stimulation with Gp61-80 peptide and Brefeldin A (GolgiPlug™, BD Biosciences). No peptide controls were treated under the same conditions supplemented with Brefeldin A but without Gp61-80 peptide ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were washed twice with HBSS-5%FBS and once with PBS and analysed by flow cytometry (BD FACScalibur, BD Bioscience).
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bioRxiv - Immunology 2023Quote: The proliferation of PBT was induced via CD3 antibody (20 ng/ml, clone: OKT3) and CD28 antibody (5 µg/ml, BD Biosciences 555725) bound to the surface of a 96-well Nunc MaxiSorpTM microtiter plate ...
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bioRxiv - Genomics 2023Quote: ... were then sorted into a 384-well plate containing 5 μL of autoclaved source water using FACS (BD FACSMelody™ Cell Sorter, BD Biosciences). 10 µL of RLT+ lysis buffer (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... and stained for the following surface markers on ice for 30 min: CD4 (APC-Cy7, 1:100, clone RM4-5, BD Biosciences, 565650), CD8b (Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2023Quote: ... we prepared a cranial window assembly by activating the surface of a circular glass coverslip (5 mm diameter, 0.13-0.15 mm thickness; Assistent Karl Hecht) with a laboratory corona treater (BD-20ACV Electro-Technic Products). We then fused the activated glass coverslip to a cylindrical silicone plug (1.5 mm diameter ...
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bioRxiv - Cancer Biology 2023Quote: ... of 6 weeks old were inoculated subcutaneously (s.c.) with 5 × 105 cells mixed 1:1 with BD Matrigel Matrix High Concentration (BD Biosciences, Erembodegem, Belgium) previously diluted 1:1 in culture medium ...
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bioRxiv - Immunology 2024Quote: ... and Parabacteroides distasonis were grown anaerobically at 37°C in brain heart infusion (BHI) media (Oxoid, UK) supplemented with hemin (5 mg/L) and vitamin K (0.5 mg/L) (BD, Franklin Lakes, NJ). Lactobacillus johnsonii and Ligilactobacillus murinus were grown anaerobically at 37°C in de Man Rogosa Sharpe (MRS ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell pellet was suspended with FACS medium and incubated with 5 μl of Alexa Fluor 488-conjugated anti-alkaline phosphatase (BD Pharmingen, 561495) and 5 μl of APC-conjugated anti-c-KIT (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... samples were measured by a MACSQuant 18 (Figure 5) or 16 (Supplementary Figure 6) flow cytometer and analysed by FlowJo® software (BD company). Three spleen samples from lmon_0149 vaccinated mice (Figure 5 ...
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bioRxiv - Microbiology 2022Quote: ... Colonies were grown from frozen stocks by streaking on TSA-II agar plates supplemented with 5% sheep blood (BD BBL, NJ, USA). Unless otherwise stated ...
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bioRxiv - Neuroscience 2023Quote: ... for 10 min at 37 °C and 5 % CO2 before they were stained with Fixable Viability Stain 510 (1:100, #564406, BD Horizon, USA) for 15 min at room temperature (RT ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then washed with PBS+5% FBS and stained with 1:100-dilutions of AF647-conjugated anti-PAX-6 (BD Biosciences; 562249) and PerCP-Cy5.5-conjugated anti-SOX-2 (BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 μL of PBS containing 5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5% goat serum) for 1h at RT and then stained with EC specific CD31 antibody (BD biosciences, 550274, 1:25 dilution) or muscle base membrane laminin antibody (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed with PBS+5% FBS and stained with 1:100-dilutions of AF647-conjugated anti-PAX-6 (BD Biosciences; 562249) and PerCP-Cy5.5-conjugated anti-SOX-2 (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... the suspensions of Tol 5 and its mutants were placed into a 96-well polystyrene (PS) microplate (353072; Becton, Dickinson and Company) and a 96-well glass plate (FB-96 ...
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bioRxiv - Molecular Biology 2024Quote: ... pre-equilibrated in selection buffer (20 mM HEPES pH 7.4, 150 mM NaCl, 5% Maltose) and analyzed using an Accuri Flow Cytometer (BD Accuri C6 Plus). Positive selections for FACS were achieved by incubating the yeast with biotinylated HPV16 E6 and staining with Alexa Fluor 647-conjugated streptavidin and Alexa Fluor 488-conjugated anti-HA mouse monoclonal antibody (Cell Signaling ...
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bioRxiv - Systems Biology 2024Quote: ... and lawns of pAWP78-P89-20ZRfae in E.coli S17-1 (overnight) were prepared for mating on plates with Hypho media supplemented with 5% Nutrient Broth (NB Difco, BD Life Sciences). Plates were incubated for 1 day at 30° C ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated monomers of MR1-5-OP-RU or CD1d-α-GalCer were tetramerised to streptavidin conjugated to PE (SAV-PE; BD Pharmingen), BV421 (SAV-BV421 ...