Labshake search
Citations for Becton, Dickinson and Company :
1001 - 1050 of 2969 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Phycoerythrin (PE) or peridinin chlorophyll protein -cyanine 5.5 (PerCP-Cy5.5)-conjugated CD3 (BD Biosciences Cat#5 61808, BD Biosciences Cat# 55163); Pacific Blue-conjugated (PB) ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed in 3ml FACS buffer before being fixed in 1% paraformaldehyde and analyzed within 5 days using a Fortessa Flow Cytometer and BD FACSDiva Software (BD Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... Then cells were washed twice with HBSS-5%FBS and once with PBS and analysed by flow cytometry (BD FACScalibur, BD Bioscience).
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bioRxiv - Immunology 2023Quote: ... The cells were cultured at 37°C and 5% CO2 for 72 hours and then the dye dilution was analyzed using a FACSVerse flow cytometer (BD Biosciences), and data were analyzed using FlowJo software (Tree Star).
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bioRxiv - Bioengineering 2023Quote: ... and then cells were re-suspended in 150 μL of PBS with 5% FBS and analyzed on a BD FACS Canto2 flow cytometer (BD Biosciences) for PE-Cy7 expression.
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bioRxiv - Cell Biology 2022Quote: ... Samples were stimulated with the indicated agonists and labelled with a PS detecting antibody (CyTM 5 Annexin V, #559934, BD Biosciences) for 15 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... The following primary antibodies were used: Anti-CD166 mouse monoclonal antibody conjugated with PE (phycoerythrin) at 5 uL (BD Biosciences #559263), anti-LRIG1 sheep polyclonal unconjugated antibody at 2.5 ug (Invitrogen #PA5-47928) ...
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bioRxiv - Immunology 2023Quote: ... Cells were acquired on a Cytek Aurora spectral flow cytometer using Cytek SpectroFlo software (Cytek; the HD BM samples) or a LSR Fortessa X20 (special order research product with 5 lasers; BD Biosciences) and analyzed using FlowJo software (v10.8.1 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were discarded and cells resuspended in FACS buffer and tetramer-binding B cells were individually sorted using a 5-laser (355nm, 405nm, 488nm, 561nm, 640nm) FACS Aria (BD Biosciences) into empty 96-well low profile PCR plates (Labcon) ...
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bioRxiv - Bioengineering 2023Quote: ... was grown overnight (37°C, 4% H2, 5% CO2, 91% N2, <10 ppm O2) in Chopped Meat Carbohydrate Broth (BD297307, BD, USA). Genomic DNA was extracted from B ...
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bioRxiv - Systems Biology 2023Quote: ... A PDMS chamber was put on the mold and a solution of collagen rat tail I (5 mg/ml, BD Biosciences) was injected and allowed to polymerize at 37 °C for 1.5 hours ...
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bioRxiv - Biochemistry 2023Quote: ... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.
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bioRxiv - Cancer Biology 2023Quote: ... the pLCOs in suspension were centrifuged at 500 × g for 5 minutes at 4 °C and then resuspended in cold growth factor-reduced Matrigel (BD Biosciences). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... while 100 μg was subjected to chromatin immunoprecipitation (ChIP) using 5 μg of mouse anti-ASCL1 (Mash1) antibody (BD Biosciences, 556604) or 5 μg rabbit ant-OLIG2 (Olig2 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting colonies were inoculated into 5 mL of marine broth medium (BD Difco Marine Broth 2216; Becton, Dickinson and Company), which was shaken at 300 rpm and 25°C until bacterial growth was confirmed ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained with the corresponding FACS panels (Extended Data Table 5) at 4 °C for 30 minutes and sorted using BD Aria III (BD Biosciences) or MoFlo Astrios EQ (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... and either 1×106 VCaP or 5×105 22Rv1 cells suspended in 100 μl of PBS with 50% Matrigel (BD Biosciences) were implanted subcutaneously into the dorsal flank on both sides of the mice ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Bioengineering 2023Quote: ... VA) was cultured on plates of BBL Trypticase Soy Agar with 5% sheep blood (BD Trypticase Soy Agar II) (BD Biosciences) in a 37°C humidified 5% CO2 environment for 10-11 hours and diluted in 0.9% sodium chloride to the desired optical density ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x with FACs buffer at 1000xg for 5 minutes at room temperature and analyzed on a Canto II (BD Biosciences) at the Flow Cytometry Shared Resource at the University of Arizona or on a BD Fortessa ...
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bioRxiv - Cell Biology 2023Quote: ... Cas9-BFP+ cells were sorted for top 5% of the brightest GFP signal simultaneously on two BD FACSAria II Cell Sorters (BD Biosciences) equipped with blue 488 nm and violet 405 nm lasers ...
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bioRxiv - Immunology 2023Quote: ... Effector cells were incubated with K562 and Nalm6 variants for 4 hours at a ratio of 5:1 with addition of Brefeldin A (GolgiPlug, BD Biosciences). Next ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in 1X PBS with 1X protease/phosphatase inhibitor cocktail in and filtered through a 5 ml cell-strainer capped FACS tube (BD Falcon). Cell sorting was performed on BD Biosciences FACS Aria Fusion ...
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bioRxiv - Immunology 2024Quote: ... and each tube was stained with 5 μL of APC-Cy7 Mouse Anti-Human CD31 (PECAM-1) antibody (clone WM59, BD Biosciences) and 5 μL of APC anti-human CD144 (VE-Cadherin ...
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bioRxiv - Molecular Biology 2024Quote: ... were stimulated by incubation with 5 µg ml-1 anti-IgM F(ab’)₂ fragment antibody (115-006-020; Jackson Immuno Research) with or without 5 µg ml-1 anti-CD40 antibody (553787, BD Pharmingen), with 100 ng/ml MPL-A (Invivogen ...
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bioRxiv - Immunology 2024Quote: ... Data was acquired using a Cytek Aurora 5-laser flow cytometer or ImageStream cytometer (CytekBio) and subsequently analyzed FlowJo V10 (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... Cells were spun down at 300 g for 5 min and supernatant was collected for analysis by mouse inflammatory cytokine bead array (CBA, BD Biosciences). Cells were resuspended and stained for CD4 and analyzed for proliferation based on CFSE decrease (Fig SI 3).
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bioRxiv - Immunology 2024Quote: ... cultured pMacs in 24-well plates were incubated in primary anti-MHC-II monoclonal antibody (mAB; BD Biosciences, see Table 5) at a final concentration of 5μg/mL for 20 minutes at 4°C in FACS buffer containing 10% normal goat serum ...
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bioRxiv - Microbiology 2024Quote: ... and its mutants were all grown at 37°C with 5% CO2 with either Todd-Hewitt medium (TH medium; BD Biosciences), chemically defined media (CDM) ...
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bioRxiv - Immunology 2024Quote: ... cultured pMacs in 24-well plates were incubated in primary anti-MHC-II monoclonal antibody (mAB; BD Biosciences, see Table 5) at a final concentration of 5μg/mL for 1 hour at 37°C in FACS buffer to allow for the antibody-binding and subsequent uptake of MHC-II complexes ...
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bioRxiv - Immunology 2024Quote: ... Cells were then cultured at a density of 5-20k live B cells/well in 96-well Microtest U-bottom plates (BD Falcon)for 8 days with 100 ng/mL anti-human CD40 antibody (clone G28.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... Xenografts were established by subcutaneous injections of PC-9 cells expressing ERBB4 variants (5 x 106 cells in 50% Matrigel matrix (#354234, BD Biosciences)) in the right flank of each mouse ...
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bioRxiv - Cancer Biology 2024Quote: ... and monocyte block for 5 minutes prior to staining with the 20-antibody cocktail (Supplemental Methods) in Brilliant Stain Buffer Plus (BD Biosciences) and FACS buffer (PBS ...
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Effective targeting of CD19 positive primary B-ALL cells using CAR-NK cells generated with mRNA-LNPsbioRxiv - Cancer Biology 2024Quote: ... NK cells were transfected with 1-2μg EGFP mRNA-LNP per 106 cells on day +7 in upright culture flask at 37°C in a humidified incubator with 5% of CO2 and then harvested for flowcytometry analysis with BD FACSLyric system (BD Biosciences). Optimal transfection conditions were determined using EGFP mRNA-LNP expression results ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was taken after 5 hours (day 0) and strain ratios were immediately measured using a flow cytometer (HTS, Fortessa, BD Bioscience). Cultures were then propagated with 1:1000 dilutions into fresh medium every 24 hours for other 2 days ...
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bioRxiv - Immunology 2023Quote: ... or anti-mouse IgA (BD Biosciences; C10-3; at 3 μg/ml) in PBS overnight and then washed three times with PBS and blocked with Tris-buffered saline containing 1% BSA (TBS/BSA ...
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bioRxiv - Immunology 2021Quote: ... activated-Caspase-3 (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... 3 (BD Biosciences #211693), 5 g/L sodium chloride ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mac-3 (BD Pharmingen), MMP-2/-9 (Novus Biologicals) ...
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bioRxiv - Microbiology 2022Quote: ... and 7-AAD nucleic acid dye (BD Biosciences) as a viability marker ...
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bioRxiv - Microbiology 2023Quote: ... Amoxicillin-clavulanic acid 20/10 μg (BD BBL) and Polymyxin 300 UI (BBL).
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bioRxiv - Microbiology 2024Quote: ... pH 7) supplemented with 0.5% Casamino acids (BD), 1 mM MgCl2 ...
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bioRxiv - Bioengineering 2024Quote: ... 0.2% w/v Bacto casamino acids (BD Biosciences), 2 mM MgSO4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Between 2 - 4×105 events were collected per sample within 3 hours after staining on a LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were immediately split into 3 fractions: 2 ml were transferred to heparin and EDTA tubes respectively (both BD, Franklin Lakes, New Jersey). Heparin samples were then centrifuged (1000x g ...
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bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
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bioRxiv - Immunology 2020Quote: Female C57BL/6J mice aged 8 – 12 weeks were injected intraperitoneally with 2 ml each of 3% sterile thioglycollate (BD Biosciences, catalog # 211716) medium ...
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bioRxiv - Cancer Biology 2023Quote: ... of both preparations was determined by titration onto target cell lines and quantification of the percentage of BFP+ cells 2–3 days post-transduction by flow cytometry (BD Biosciences LSR II).
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bioRxiv - Immunology 2022Quote: ... 10% FCS and incubated with the indicated pool of peptides (5 μg/ml final concentration of each pool) and brefeldin A (1 μg/ml; BD Pharmingen, USA) at 37°C for 12-16 hours ...
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bioRxiv - Immunology 2022Quote: ... distasonis D13 strain and Bacteroides fragilis (ATCC® 25285) were cultured in anaerobic culture broth (Tryptic Soy Broth supplemented with 5 µg/ml Hemin (BD Biosciences) and 1µg/ml Vitamin K1 (VWR ...