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Citations for Becton, Dickinson and Company :
951 - 1000 of 1014 citations for Recombinant Rat Gfra1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were stained on ice for 30min in FACS buffer with 1:100 dilutions of biotin-conjugated-HA and purified rat anti-mouse CD16/CD32 (Mouse BD Fc Block™, Clone 2.4G2, BD Biosciences), then stained on ice for 30min in FACS buffer with 1:1000 dilution of allophycocyanin (APC)-conjugated streptavidin ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated for 45 min at 4°C with rocking with 1:250 of each of the following antibodies: Negative selection markers: BV421 Rat anti-mouse anti-CD31 (562939, BD Biosciences), and BV421 Rat anti-mouse CD45 (563890 ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently labeled for 30 minutes on ice with the following antibodies and reagents: PE-conjugated rat anti-mouse Siglec-F (E50-2440; BD Biosciences), PE-TxR or PE-Cy5-conjugated rat anti-mouse CD11b (M1/70.15 ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
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bioRxiv - Systems Biology 2023Quote: ... A PDMS chamber was put on the mold and a solution of collagen rat tail I (5 mg/ml, BD Biosciences) was injected and allowed to polymerize at 37 °C for 1.5 hours ...
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bioRxiv - Immunology 2023Quote: ... mock or INHBA overexpression CT26 cells were directly analyzed for PD-L1 expression (BV650 Rat Anti-Mouse CD274; 740614, BD Bioscience) by flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... mock or INHBA overexpression tumor cells (CT26 and MC38) and shCtrl or shINHBA CT26 cells were analyzed for IFNGR1 (BV421 Rat Anti-Mouse CD119; 740032, BD Bioscience) and IFNGR2 expression (Hamster Anti-Mouse IFNGR2 Monoclonal antibody ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed two times with 150 µL PBS (centrifuged 5 min at 400 × g at 4 °C) and resuspended in 50 µL Fc-block (1:50, Purified Rat Anti-Mouse CD16/CD32, BD, #553142) diluted in FACS buffer (1x PBS/2% FBS/2mM ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibody staining for Cxcr4 was performed for 20 mins on ice using 1:100 APC-conjugated Rat anti-Mouse CD184/Cxcr4 (BD Pharmingen) in FC buffer and cells were then washed twice in FC buffer and resuspended in 1 μg/ml DAPI in FC buffer at ∼1-1.5 x 1E7 cells/ml to label dead cells and run the suspension through a cell strainer ...
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bioRxiv - Neuroscience 2020Quote: ... one-in-ten sections were incubated with different anti-BrdU antibodies (BrdU & CldU, rat primary antibodies at 1/200 Accurate Chemical; IdU, mouse primary antibodies at 1/200, BD Biosciences). Sections were also incubated with Zif268 (rabbit,1:500 ...
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bioRxiv - Immunology 2021Quote: 12-week old mice were sensitized by intradermal injection of 100ug rat collagen type II (COL2) in 100ul of a 1:1 emulsion with Complete Freund’s adjuvant (BD, Difco, MI, USA) and 10mM acetic acid at the base of the tail ...
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bioRxiv - Microbiology 2021Quote: ... 2×106 lymph node cells were stained with the following monoclonal antibodies: rat anti-mouse CXCR5-biotin (clone 2G8, 1:100, BD, cat#551960), streptavidin PECy7 ...
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bioRxiv - Microbiology 2019Quote: ... for 1 h or 2 h and then subsequently incubated for 30 min on ice with an APC-conjugated rat anti-mouse IgG (BD Biosciences, CA). Stained cells were analyzed using a BD AccuriTM C6 Plus with a Sampler flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... 1 µL of mAbs were also added to one drop of compensation beads (Anti-Rat Ig, κ/Negative Control Compensation Particles Set (BD Biosciences)) to prepare single-stain controls for compensation ...
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bioRxiv - Immunology 2020Quote: ... Single cell suspensions from mouse tissues or tumors were incubated for 15 minutes at ambient room temperature with rat anti-mouse Fc Block™ (BD Pharmingen). Cells were then incubated with primary antibodies for 30 minutes at 4ºC in the dark ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody dilutions were prepared as follows in 5% NGS in 1X PBST: rat anti mouse CD11b clone M1-70 (BD Pharmingen; #553308) – 1:100 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for 15 min at 37°C before incubation with primary antibodies Alexa Fluor 647 Rat Anti-mouse CD31 (BD Pharmigen cat:563608) for CD31 cell labeling and Alexa Fluor 647 Rat IgG2a,k Isotype control (BD Pharmigen cat:557690) ...
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bioRxiv - Developmental Biology 2021Quote: ... for 60 min, the sections were reacted with the primary antibody (for CD31, 1:100 rat monoclonal anti-CD31 (BD Biosciences, 550274); for Cytokeratin ...
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bioRxiv - Cancer Biology 2021Quote: ... The fibres were stained with primary antibodies (Rat anti-BrdU Abcam ab6326 1:250, Mouse anti-BrdU BD Biosciences 347580 1:100) overnight and with secondary antibodies (Alexafluor 555 goat anti-rat 1:500 ...
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bioRxiv - Immunology 2020Quote: ... and BV421 rat anti-mouse TNF-α BD Biosciences in a total of 100 μL of 1× Perm/Wash Buffer (BD Biosciences). After two washes ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and centrifuged at 1000rpm for 5-10min and stained with APC-Cy™7 Rat Anti-Mouse CD3 (clone: 17A2, Cat # 560590, BD Biosciences), FITC Rat Anti-Mouse CD4 (Clone ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-Tuj1 (gift from Tony Spano, University of Virginia, 1:250) and rat anti-Icam-II (BD Pharmingen, 553325, 1:500). Secondary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... 1.4 x 105 cells/10 μl were combined with equal amounts of Type I Rat Tail collagen (BD Bioscience, San Jose, CA) and neutralising solution (100 mM HEPES dissolved in 2xPBS ...
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bioRxiv - Immunology 2022Quote: ... 1 μg of purified rat anti-mouse CD16/CD32 was added to each pool (Mouse BD Fc Block, BD Biosciences, cat. 55142) and incubated for 30 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 1 μg of purified rat anti-mouse CD16/CD32 was added to each pool (Mouse BD Fc Block, BD Biosciences, cat. 55142) and incubated for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated in media containing 10 µg/mL blocking antibody (Purified Rat Anti-Human CD29 Clone Mab 13, BD Biosciences, #552828) or equivalent concentration non-targeting isotype control (Purified Rat IgG2a κ Isotype Control Clone R35-95 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with FACS staining buffer (PBS supplemented with 2% fetal bovine serum and 1 mM EDTA) and incubated with Fc receptor blocker purified rat anti-mouse anti-CD16/CD32 (BD Biosciences, 553142) for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 5X106 cells per mL were washed with cold PBS and blocked in PBS with Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Biosciences) for 15 min at 4°C in the dark ...
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bioRxiv - Microbiology 2024Quote: ... Unspecific binding of antibodies was blocked by incubating the samples with 3% rat serum in BD staining buffer (BD Biosciences, Heidelberg, Germany) for 5 minutes at RT ...
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bioRxiv - Neuroscience 2019Quote: ... injection with α-Ly6G or isotype control antibodies (IP 4 mg/kg; α-Ly6G, clone 1A8, No. 561005; Iso-Ctr, Rat IgG2a, κ, No. 553929; BD Biosciences). Animals were 9-10 months of age at the start of the experiment.
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bioRxiv - Cell Biology 2021Quote: BMSCs were harvested from both femurs of the rats and sorted using a FACS Aria II flow cytometer (BD Biosciences, New Jersey, USA). Bone marrow cells were flushed from the bone marrow cavity after which a red blood cell lysis buffer was used to eliminate red blood cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was prevented using the ells were stained using the BD Pharmingen Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Clone 2.4G2 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were transferred to a 96 well U-bottom plate and Fc receptors on cells were blocked with Rat anti-mouse Fc-Block CD16/CD32-Clone 2.4G2 (BD Pharmigen, San Jose, CA) on ice for 15 minutes ...
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bioRxiv - Immunology 2019Quote: ... Cytokine concentration was quantified in the supernatants by cytometric bead array (CBA) according to the manufacturer’s instructions from the Mouse/Rat Soluble Protein Flex Set System kit (BD Biosciences Pharmingen, Oxford, UK). CBA data was analysed on a MACSQuant analyser with MACSQuantify™ Software and the CBA analysis software package (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... cells were stained with PerCP/Cy5.5 or BV421-conjugated anti–mouse CD4 rat monoclonal antibody (RM4-5; BD Biosciences, Franklin Lakes, NJ, USA). The cells were then fixed and permeabilized with Cytofix/Cytoperm reagent (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in x μL of unlabeled CD16/32 mAb Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™), BD Pharmingen 553141) ...
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bioRxiv - Cell Biology 2020Quote: ... and rat α7 integrin-APC (1/1000; AbLab, Vancouver, Canada) and sorted using a FACS Aria II (BD Biosciences, San Jose, CA, USA). Satellite cells were isolated as CD31− ...
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bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
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bioRxiv - Genomics 2022Quote: ... Each surface staining and intracellular staining step was accompanied by a rat FcBlocking step via an anti-CD32(BD Bioscience Clone: D34-485). Events were acquired on a 5-laser custom BD Fortessa X20 analyzer ...
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bioRxiv - Immunology 2023Quote: ... cells were washed in perm buffer (PBS, 0.5% BSA, 0.1% Saponin) and stained for IFN-γ (APC rat anti- mouse IFN-γ; 1:100; BD Biosciences, Cat. 554413) and TNF (PE rat anti-mouse TNF alpha ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
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bioRxiv - Immunology 2023Quote: ... and the cells were stained with antibodies specific for the following proteins: CD4 (PE Rat Anti-Mouse CD4; Clone RM4-5; BD Biosciences; Cat # 553049), H-2Dd (Anti-mouse H-2Dd antibody ...
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bioRxiv - Cancer Biology 2023Quote: Epithelial cell suspensions were stained for viability using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (Thermo #L23105) and BUV737 Rat Anti-Mouse CD45 Clone 30-F11 (BD Biosciences # 568344) and analyzed using a 5-laser Cytek Aurora.
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Microbiology 2024Quote: ... The homogenized cell suspension was washed and resuspended in FACS buffer containing the PE-conjugated Rat anti-mouse F4/80 antibody (BD Horizon, Cat-565411) or eFluor450-conjugated anti-mouse CD11c antibody (eBioscience) ...
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bioRxiv - Neuroscience 2024Quote: ... The cover slips were then permeabilized with 0.3% Triton X-100 and sequentially stained with rat anti-CD44 antibody (BD Biosciences, 550538; 1:200), and corresponding Alexa 488-conjugated secondary antibodies (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... were plated in buffer (PBS with 0.5% bovine serum albumin and 1 mM EDTA) and non-specific Fc binding was blocked via incubation with mouse anti-rat CD32 (BD Biosciences #550271; 1:200) for 30 minutes on ice ...
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bioRxiv - Microbiology 2021Quote: ... and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution, BD Pharmingen, cat No. 559864). Cell nucleus was stained with DAPI ...
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bioRxiv - Cancer Biology 2019Quote: Glioma TMA sections (4 μm) were stained using a rat monoclonal primary antibody to the integrin β4 subunit (CD104; clone 439-9B; BD Pharmingen, San Jose, CA) at a concentration of 5μg/ml according to a previously described protocol (11) ...