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Citations for Becton, Dickinson and Company :
901 - 950 of 1014 citations for Recombinant Rat Gfra1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Antibodies for immunostaining were diluted in PBS with 3% BSA and 0.1% Tween-20 as follows: 1:800 rat anti-mouse CD31 (BD, 553370), 1:1000 rabbit anti-ERG (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... AKP-Z tumor derived cells were stained using the following antibodies: rat anti-mouse CD45-BV421 (clone 30-F11, 563890, BD Biosciences), rat anti-mouse CD31-BV421 (clone 390 ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight in blocking solution with the following primary antibodies (1:100): rat anti-mouse SiglecF (E50-2440, 552126 BD Biosciences), Armenian hamster anti-mouse CD80 (16-10A1 ...
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bioRxiv - Immunology 2021Quote: ... the brain sections were incubated with 3% serum for 1 hour followed by over-night incubation with primary antibodies (1:1000; Rat anti-mouse CD4 from BD Bioscience) in a humidified chamber at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Protein synthesis of Syndecan-1 was shown by immunocytochemistry using a polyclonal antibody against murine Syndecan-1 (CD138) clone 281-2 (1:50, rat anti-mouse, BD Pharmingen) in co-cultured cells after fixation with ice-cold 99% methanol and heparitinase III (2.4mIU/ml ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and incubated overnight at 4°C with the fluorochrome-conjugated antibodies and unconjugated rat and mouse IgG in Perm/Wash solution (BD Biosciences), which was followed by additional 5 min incubation in Perm/Wash solution without antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μl of platelet suspension was mixed with 40 μl of staining solution (HEPES-Tyrode buffer supplemented with 2.5 mM CaCl2, 1:50 PE rat anti-mouse CD41 antibody (BD Biosciences 561850), and 3:50 FITC Annexin V (BD Biosciences 556419)) ...
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bioRxiv - Immunology 2019Quote: Lymphocytes or purified splenic B cells were washed in PBS with 1% BSA and incubated with 1mg/ml purified rat anti-mouse CD16/CD32 (Fc block, BD Pharmingen) at 4°C for 15 min ...
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bioRxiv - Microbiology 2019Quote: ... the cells were washed once with FACScan buffer and then stained with PE-conjugated monoclonal rat anti-mouse CD4 (BD Pharmingen) and/or APC-conjugated monoclonal rat anti-mouse CD8 (eBioscience) ...
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bioRxiv - Immunology 2019Quote: ... Cells were further incubated for an additional 30 minutes on ice with a mixture of the following antibodies: Alexa Fluor 700 rat anti-mouse CD45R (B220, clone RA3-6B2, BD Biosciences), FITC rat anti-mouse CD43 (Clone S7 ...
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bioRxiv - Immunology 2019Quote: ... IgM-negative B cells were purified from lymphocytes using biotinylated anti-rat IgM (Clone G53-238, BD Biosciences, San Jose, CA) and magnetic separation (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used for analysis of the H2B-GFP LR population (Figure 5): rat anti- Cd74-BUV395 (Clone In-1, BD, 740274), rat anti-Cd45-BUV563 (Clone 30-F11 ...
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bioRxiv - Cancer Biology 2019Quote: ... spheroids were washed in PBS and incorporated into 3D type I collagen lattices consisting of non-pepsinized rat-tail collagen (BD Biosciences) at a final concentration of 4 or 6 mg/ml for 4T1 or MMT cells ...
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bioRxiv - Neuroscience 2020Quote: ... Mononuclear cells from the remaining five brains were labeled with APC-Cy7 rat anti-CD11b antibody (BD Pharmingen, Cat. No. 557657) for 30min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were blocked with 10% goat serum in PBS with sodium azide at RT for 15 mins before incubating with anti PD-L1 antibody (1:100; clone 6C11-3A11) or isotype rat IgG2a control (1:50; BD Biosciences) at RT for 30 mins21 ...
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bioRxiv - Cancer Biology 2021Quote: ... rat anti-Foxp3 (1:100 dilution, clone FJK-16s, eBioscience) and mouse anti- CCR4 (1:100 dilution, clone 1G1, BD Biosciences), were applied at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... rat anti-Foxp3 (1:400 dilution, clone FJK-16s, eBioscience) or mouse anti-CCR4 (1:100 dilution, clone 1G1, BD Biosciences), at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT were followed by a 1 h incubation with biotinylated goat anti-rat (559286, BD biosciences) 1:100 in TNB ...
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bioRxiv - Microbiology 2022Quote: ... Cells were subsequently fluorescently labeled for 30 minutes on ice with the following antibodies and reagents: PE-conjugated rat anti-mouse Siglec-F (E50-2440; BD Biosciences), PE-TxR-conjugated rat anti-mouse CD11b (M1/70.15 ...
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bioRxiv - Developmental Biology 2022Quote: ... the secondary antibody reaction was carried out using FITC Rat anti-mouse IgM antibody (553408; BD Biosciences, Franklin Lakes, NJ, USA) at 1:200 dilution for 30 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: About 2×105 cells per sample were blocked using Purified NA/LE Rat Anti-mouse CD16/CD32 Clone 2.4G2 antibody (BD Biosciences, #553140) followed by incubation with 1 μl of specific antibodies for 30 min on ice in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with rat anti-BrdU/CIdU (BU1/75) monoclonal antibody (Novus, NB500-169) and mouse anti-IdU monoclonal antibody (BD, 347580). The secondary antibodies were AlexaFluor Cy3-conjugated goat anti-rat and AlexaFluor 488-conjugated goat anti-mouse respectively ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked with Purified Rat Anti- Mouse CD16/CD32 Mouse BD Fc Block™ (2.4G2, BD Pharmingen, 1:1000). Cell surface antigens were stained in PBS containing 2 % (vol/ vol ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Cells were then consecutively stained with FCRLs monoclonal rat antibody (Butovsky et al., 2014) (30 min), goat anti-rat APC antibody (Biogegend, # 405407) (20 min) and Cd11b PeCy7 antibody (BD, #553142) (20 min ...
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bioRxiv - Developmental Biology 2019Quote: ... the cells were resuspended with blocking buffer (5% BSA in DPBS) and stained with PE-Cy7 rat anti-CD31 (1:400, 561410, BD biosciences), APC mouse anti-EpCAM (1:400 ...
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bioRxiv - Immunology 2019Quote: ... After washing in PBS and incubated for 1 hour at RT with secondary donkey-anti-rat rhodamine red 1:100 (both from BD Pharmingen) in antibody buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was aspirated and the cell pellet was resuspended in 200uL of FACS buffer (PBS, 0.5% BSA, 2mM EDTA, 20mM Glucose) with 1:50 PE Rat Anti-Human CD49f antibody (BD Biosciences; 555736) and incubated on ice for 20 minutes ...
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bioRxiv - Pathology 2020Quote: One day before VBP re-seeding (Day 9), collagen constructs were prepared (15, 41) by combining high-concentration rat tail collagen (4mg/ml; 80% final volume BD Biosciences) and 10xDMEM (10% final volume ...
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bioRxiv - Genomics 2019Quote: ... 500ng-1ug of sample was spotted on the membrane and stained with rat BrdU/CldU 1:500 (Bio Rad OBT0030S) or mouse BrdU/IdU 1:500 (BD 347580) in 3% BSA in TBST for 1h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Primary antibodies diluted as appropriate were: the rat anti-mouse MAC-3 antibody Clone M3/84 (1:100 dilution, BD Pharmingen), rabbit polyclonal NOS2 antibody (#ab15323 ...
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bioRxiv - Microbiology 2021Quote: ... Sections were stained using the following antibodies: rat anti-mouse Ly-6C/Ly-6G (RB6-8C5, BD Bioscience, #550291, 1/200) followed by AlexaFluor647-conjugated goat anti-rat IgG (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... cells were washed with 1x permeabilization buffer and stained with intracellular cytokines (IFN-L (BV421 Rat Anti-Mouse IFN-γ, Clone: XMG1.2, cat # 560660, BD Biosciences) for 30 mins at 4°C ...
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bioRxiv - Immunology 2022Quote: Single cells suspensions were incubated a mixture containing 50 µg/ml rat anti-mouse CD16/CD32 (BD Biosciences, Franklin Lakes, USA), normal Syrian hamster serum (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... the cell suspension was then incubated on two PDGFRα plates coated with rat anti-PDGFRα IgG (BD Biosciences, 558774; 1:500). The adherent cells were washed with PBS to remove all antigen-negative nonadherent cells ...
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bioRxiv - Immunology 2022Quote: ... The supernatant was mixed with beads and subsequently incubated with an isotype control (rat IgG1 κ, BD Pharmingen, San Diego, CA) or biotinylated 6D11 (4 °C ...
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bioRxiv - Immunology 2022Quote: ... Mouse and rat anti-human monoclonal antibodies (mAbs) used in this study included: IFNγ FITC (clone 25723.11, BD Biosciences, cat# 340449), TCRαβ Percp 710 (clone IP26 ...
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bioRxiv - Cell Biology 2022Quote: Freshly dissected LW whole mount preparations from 6 wild-type and 4 Lrp2-/267 mice were incubated with rat anti-CD24 conjugated with Phycoerythrin (PE; 1:100; BD Pharmingen) in Neurobasal medium (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Phenotypic analysis was performed with the following antibodies: Rat anti-mouse active β1-integrin (active CD29 Clone 9EG7, 553715; BD Biosciences); Alexa647-Armenian hamster anti-mouse β1-integrin (total β1-integrin 102214 ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD8 APC (1:100, catalog no. 553035; BD Biosciences); rat anti-CD45 PE (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... The CldU and IdU tracks were detected by IF with anti BrdU antibodies (rat for CldU, Abcam ab6326, and mouse for IdU, BD 347580). Fiber integrity was assessed with a mouse anti-ssDNA antibody (Millipore MAB3034) ...
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bioRxiv - Physiology 2024Quote: ... The 2X stain cocktail included the following antibodies (final dilutions are all 1:300 unless otherwise indicated): rat anti-mouse SiglecF-BV421 (1:400, clone E50-2440, cat# 562681 BD Horizon), rat anti-mouse/human CD11b-Pacific Blue (clone M1/70 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were stained with rabbit anti-GRA7 (1:5000) (gift from John Boothroyd, Stanford) and rat anti-LAMP1 (1D4B, BD Biosciences) (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... The antibodies employed for ELISA were biotin-conjugated rat anti-mouse IgG2a (clone R19-5, BD Biosciences, Denmark cat no. 550332) and anti-mouse IgG conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2023Quote: ... 0.25% Triton PBS before incubation overnight at 4°C with the following antibodies: Rat monoclonal to mouse Sca1 (740450, BD Biosciences), Rabbit monoclonal to mouse SOX9 (ab185230 ...
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bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were stained with Alexa Fluor 647-conjugated rat anti-CD49f antibody (5% v/v in a 100 µL reaction; BD Biosciences) for 25 minutes at 37°C on an orbital shaker at 200 rcf and resuspended in DPBS containing 0.5% bovine serum albumin and 50 units/mL DNase I (Sigma Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... The cell pellet was resuspended in 200 μl ice-cold DMEM and incubated sequentially on ice with purified rat anti-mouse FDC-M1 (BD Biosciences) or rat IgG2c ...
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bioRxiv - Biophysics 2022Quote: ... samples were first resuspended in 15.5 μL PBS/0.1% adBSA and incubated with 0.5 μg of rat anti-mouse CD9-PE (Clone: KMC8, IgG2a, κ lot 7268877, BD Biosciences) or matched isotype antibodies (Rat IgG2a ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: Lymph node single cell suspension of 2.5×106 cells was incubated for 20 min at 4°C with a purified rat IgG2b anti-mouse CD16/CD32 receptor antibody (BD biosciences). Cells were stained with fluorochrome-conjugated antibodies against CD9 ...
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bioRxiv - Molecular Biology 2023Quote: ... For BrdU staining we used either a rat anti-BrdU (1:1000, Eurobio clone BU1/75) or mouse anti-BrdU (1:1000, BD 347580) antibodies 2 hours at RT (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... together with PE-anti-PD-L2 (clone TY25, 1:20) and its isotype control PE-Rat IgG2a,κ (clone R35-95, 1:20) were purchased from BD Biosciences ...