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Citations for Becton, Dickinson and Company :
951 - 1000 of 1166 citations for CD45 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... CD45- LYVE1- LY76- PDPN- EPCAM- PI- CD31+ CD146+ cells were sorted using a BD FACSAria Fusion Flow Cytometer (BD Biosciences, Heidelberg, Germany). Immediately after sorting ...
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bioRxiv - Immunology 2024Quote: ... Surface staining was carried out for 30 minutes at 4°C with the following fluorochrome-labeled anti-mouse Abs: APC/Cy7-CD45 (BD Bioscience, #557659), PE/Cy5-B220 (eBioscience ...
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bioRxiv - Immunology 2024Quote: ... three LNs (for patients ccp2 and ccp3) and two replicates of PBMC were stained with a panel of fluorescent antibodies: CD45-PerCP/Cy5.5 (BD 564105, clone HI30), CD38-PE (BD 555460 ...
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bioRxiv - Cell Biology 2024Quote: ... BAK (rabbit, BD Pharmingen ...
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bioRxiv - Immunology 2021Quote: CD47 expression or cross-dressing on cells was determined by direct staining with BV786-conjugated anti human specific CD47 mAb B6H12 (abbreviated anti-hCD47; BD Bioscience) or PE-conjugated anti-human CD47 mAb CC2C6 (with cross-reactivity to pig CD47 and thus ...
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bioRxiv - Immunology 2021Quote: ... then sorted GFP+ cells were expanded and assessed for CD47 expression by staining with BV786-conjugated anti-hCD47 mAb B6H12 (BD Bioscience) and PE-conjugated anti-hCD47 mAb CC2C6 (Biolegend) ...
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bioRxiv - Molecular Biology 2021Quote: ... run on PAGE together with input sample (1:20 of amount of immunoprecipitated proteins) and blotted with anti-PARP1 (Mouse Mab 551025 BD Pharmigen), anti-TRF1 (Rabbit Pab sc-6165 ...
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bioRxiv - Immunology 2021Quote: ... stained with anti-CD117-PECy7 mAb (5 μg.mL−1) and viability dye and sorted by a FACSMelody™ cell sorter (BD biosciences). For immunofluorescence experiments ...
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bioRxiv - Immunology 2021Quote: ... Binding of mAbs to CTLA-4 on the cell surface was determined using LSR II (BD Biosciences, San Jose, CA, USA) or CytoFLEX LX (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... The membranes were then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk for 2 h ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk in each channel for 2 h ...
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bioRxiv - Immunology 2021Quote: ... Resting- or in vitro-generated effector CD4+ or CD8+ T cells (105 cells/well) were stimulated with 1 μg/ml plate-bound anti-mouse CD3 mAb (BD Bioscience) in combination with different concentrations of P3CSK4 ...
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bioRxiv - Immunology 2020Quote: ... Spleen B cells were analyzed for BrdU incorporation (by anti-BrdU mAb staining) and DNA contents (by 7–AAD staining after cells were permeabilized) using the APC BrdU Flow kit® (BD) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Mouse and rat anti-human monoclonal antibodies (mAbs) used in this study included: IFNγ FITC (clone 25723.11, BD Biosciences, cat# 340449), TCRαβ Percp 710 (clone IP26 ...
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bioRxiv - Immunology 2022Quote: ... Acquisitions were done on a FACS Canto II flow cytometer (Becton Dickinson) with the following mAbs: Fluorescein (FITC)-coupled 145-2C11 (anti-CD3, BD Biosciences), FITC-coupled HL3 (anti-CD11c ...
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bioRxiv - Immunology 2022Quote: ... cells were washed once with FACS buffer and stained with mAb 1A5 or 3F5 conjugated to AF647 or appropriate isotype controls (BD Biosciences) for 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... or anti-β2 mAb IB4 (10 μg/ml) followed by Alexa Fluor 488-conjugated secondary antibody and analyzed using a FACScan (BD Biosciences) and Attune NxT flow cytometer (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... Tconv cells and Treg cells in different tissues from DT- and PBS-treated Foxp3DTR mice or from αCD3 mAb-treated or Citrobacter-infected Treg-KO and WT littermates were sorted on a FACSAria Fusion cell sorter (BD Biosciences) with a purity of greater than 95% ...
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bioRxiv - Immunology 2023Quote: 50 × 103 effector cells were cultured alone or together with 5 × 103 tumour cells for 4 h at a 10:1 ratio in the presence of anti-CD107a-PE mAb (clone: H4A3; BD Biosciences) and monensin (3 μM ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with a cocktail of mAbs and four fluorochrome-conjugated spike proteins in staining buffer (2% FBS/PBS) supplemented with Brilliant Stain Buffer Plus (BD Biosciences). The stained cells were fixed (Lysing Solution ...
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bioRxiv - Developmental Biology 2022Quote: ... 1:10000), anti-dendritic cell mAb: anti-CD11c (N418)-FITC (11-0114-81, eBioscience; 1:20) using LSR II (BD Biosciences). For OC differentiation ...
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bioRxiv - Immunology 2023Quote: Cells were surface stained with fluorochrome-conjugated mAbs to examine the expression of the phenotypic markers CD3 (clone UCHT1, BD Pharmingen), CD4 (RPA-T4 ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with fluorochrome-conjugated monoclonal antibodies (mAbs) diluted at 1:200 into a 1:3 solution of Brilliant Staining Buffer (BD 563794): Cell Staining Buffer ...
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bioRxiv - Neuroscience 2020Quote: ... anti-O4 (130-118-978) (Milteny Biotec, Bergisch, Germany), anti-O1 (FAB1327V) (Novous Biologicals, Littleton, CO) and anti-CD45 (OX-1 (ROU)) (BD, Franklin Lakes, NJ). Dead cells were excluded using near IR Live/Dead (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... Cells were labeled with the following antibodies: 1:400 dilution of Anti-mouse CD45 antibody conjugated to APC-Cy7 (BD Biosciences, Cat#: 557659), 1:200 dilution of Anti-mouse TER-119 antibody conjugated to APC-Cy7 (BD Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... Following overnight incubation the plate was washed 3 times with DPBS and then incubated with 20 µl anti-CD45 antibody (BD Pharmingen, catalog #550539) in 12 ml of 0.2% BSA for a minimum of 2 hours ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: Epithelial cell suspensions were stained for viability using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (Thermo #L23105) and BUV737 Rat Anti-Mouse CD45 Clone 30-F11 (BD Biosciences # 568344) and analyzed using a 5-laser Cytek Aurora.
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bioRxiv - Microbiology 2023Quote: ... live CD45+ cells were sorted into 0.1% bovine serum albumin (BSA) in DPBS by a FACSAria IIu Cell Sorter (BD Biosciences, Franklin Lakes, NJ). Sorted cell suspension were pelleted at 350 x g for 5 minutes at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The single cell suspension obtained from human muscle was stained with a cocktail containing PE-Cy5 anti-human CD45 (BD Pharmingen, cat#555484), Percp-Cy5.5 anti-human CD31 (BioLegend ...
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bioRxiv - Microbiology 2022Quote: In vitro CYP inhibition assay was performed using a standard commercial recombinant CYPs kit (BD-Gentest) as recommended by the company ...
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bioRxiv - Immunology 2023Quote: ... Fluorescent antibody or recombinant protein biding on fluorescent nanobeads were directly counted and measured by BD FACSCelesta™ flow cytometer.
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... resuspended in 50ul FACS buffer containing 0.5 μg anti-mouse CD16/CD32 mAb 2.4G2 (Mouse BD Fc Block, BD Pharmingen, San Diego, California), and incubated for 15 minutes 4° C ...
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bioRxiv - Immunology 2019Quote: ... 1 µL of mAbs were also added to one drop of compensation beads (Anti-Rat Ig, κ/Negative Control Compensation Particles Set (BD Biosciences)) to prepare single-stain controls for compensation ...
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bioRxiv - Immunology 2019Quote: ... and stained with CD45.2-FITC (to identify leukocytes) and cell subset specific fluorochrome-conjugated mAb (all were obtained from BD Biosciences and Biolegend). Data were acquired on a BD LSR Fortessa flow cytometer with BD FACS Diva software and analyzed using FlowJo Software version 9.5 (Tree Star ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated in media containing 10 µg/mL blocking antibody (Purified Rat Anti-Human CD29 Clone Mab 13, BD Biosciences, #552828) or equivalent concentration non-targeting isotype control (Purified Rat IgG2a κ Isotype Control Clone R35-95 ...
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bioRxiv - Immunology 2023Quote: ... and co-cultured with human CD8+ T cells at 1:1 E:T ratio in the presence of anti-CD107a-APC mAb (BD Biosciences, CA, USA). After 1 hour ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 10 ng/mL anti-CD3 mAb at 4°C overnight (Purified NA/LE mouse anti-human CD3, BD Cat# 557052). Three-fold serial dilutions of either IL-7 or a test compound were added to the cells and incubated for 4 days at 37°C ...
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bioRxiv - Immunology 2024Quote: ... For the clone that binds to the lower affinity IL-2 receptor (CD122): we incubated 5ugr mouse Il-2 (PepreoTech) to 50ugr of an IL-2 mAb (S4B6; BD or BioXcell) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... Enumeration of human CD45+ cells in spleen and bone marrow was performed by flow cytometry after staining with BV605-conjugated human-specific CD45 antibody (clone HI30, BD Biosciences, San Jose, CA) using a BD LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution, BD Pharmingen, cat No. 559864). Cell nucleus was stained with DAPI ...
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bioRxiv - Bioengineering 2022Quote: ... Macrophages were characterized for presence and absence of canonical markers using flow cytometry PE-Cy5 mouse anti-rat CD45 (BD Biosciences, Mississauga, ON, Canada), BV786 mouse anti-rat CD11b/c (BD Biosciences ...
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bioRxiv - Genomics 2022Quote: The collected cells were first stained with live/dead aqua to exclude non-viable cells from the analysis and stained with fluorophore-conjugated antibodies against surface markers anti-CD45-BV786 (BD Bioscience Clone: OX-1), anti-CD11b-V450(BD Bioscience Clone ...
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bioRxiv - Cell Biology 2024Quote: ... Cells from the stroma were sorted gating on single GFP-positive cells from PdgfrαH2Be-GFP mice or using anti-CD45-PE/Cy7 (BD Pharmingen, clone 30-F11, #552848) antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... namely anti-CD3-APC (1:5 dilution, clone SK7) and anti-CD45-PerCP (1:10 dilution, clone 2D1) (both from BD Biosciences, San Jose, CA). After incubation for 30 min at 4°C in the dark ...
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bioRxiv - Genetics 2023Quote: ... USA)/anti-rabbit IgG Alexa 488 (Jackson Immuno Research lab, West Grove, PA, USA) and 10µg/mL biotinylated anti-CD45 antibodies (BD Bioscience, Franklin Lakes, NJ, USA)/anti Rat IgG Alexa Plus 647 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/ml human recombinant epidermal growth factor (hr-EGF; 354052, BD Pharmigen, San Jose, CA, USA), 0.4 mg/ml hydrocortisone (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-Nup88 (BD Biosciences ...