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Citations for Becton, Dickinson and Company :
851 - 900 of 1166 citations for CD45 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Single cells from digested xenograft tumors were labeled using a BUV395 anti-mouse cocktail (pooled and diluted 1:40: anti-mouse H2kD [BD Biosciences, cat. 742437], anti-mouse CD45 [BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Single cells from digested xenograft tumors were labeled using a BUV395 anti-mouse cocktail (pooled and diluted 1:40: anti-mouse H2kD [BD Biosciences, cat. 742437], anti-mouse CD45 [BD Biosciences, cat. 567451], anti-mouse CD31 [BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... DCs were then isolated via flow sorting (CD3-CD19- CD45R-Ly6G-MerTK-NK1.1-Siglec-F-Ter119-, CD45+CD11c+IA/IE+) using an Influx (BD BioSciences) to a purity of 95-99% ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were counted using NucleoCounter NC250 (Chemometec) following the manufacturer instructions and CD45 positivity and viability measured on a BD Fortessa instrument (BD); more than 90% cells were viable and 95% of the cells were confirmed to express CD45 ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with live/dead aqua (Thermo) and antibodies to CD45 (FITC, clone 30-F11, BD Biosciences, RRID AB_394609), CD11b (BUV661 ...
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bioRxiv - Immunology 2022Quote: ... CD45+ skin cells or expanded MAIT cells were activated (TCR dynabeads) for 18 hours before determining RORγt expression using a specific RORγt mAb (BD Biosciences) and transcription factor staining kit (Biolegend) ...
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bioRxiv - Microbiology 2019Quote: ... 0.02% NaN3 in PBS) and stained with a FITC-conjugated anti-human HLA-DR mAb (Clone Tü36; BD Biosciences). This antibody specifically binds to a monomorphic epitope of the HLA-DR αβ complex and not the isolated α or β chains or the HLA-DP and -DQ isotypes41 ...
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bioRxiv - Immunology 2021Quote: ... The following monoclonal antibodies (mAbs) were used in this study: anti-BrdU-FITC (3D4, BD Biosciences, San Jose, CA), CD45-PE (MB4-6D6 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice in PBS and stained with mAbs to CD69 (APC, FN50, BD Biosciences USA, #555533) and CD3 (APC-Cy7 ...
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bioRxiv - Immunology 2020Quote: PBMCs (1−2×106) were stimulated with epitope-specific peptides (5 μM) and anti-CD28 mAb (0.5 μg/mL, BD) and expanded for 14 days in complete RPMI culture medium containing rIL2 (20 IU/mL ...
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bioRxiv - Cell Biology 2024Quote: ... or magnetic beads coupled to an anti-BAK antibody able to recognize the Nt region of BAK only when activated(49) (mouse mAb, clone G317.2, BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Twenty 8-11 months-old Gata1low mice were randomly divided in two groups which were treated either with vehicle (2% v/v dimethyl sulfoxide in H2O) or with biotinylated mAb RB40.34 (BD Pharmigen ...
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bioRxiv - Microbiology 2022Quote: ... PE-Cy7-anti-CD4 (clone RM4-5) and BV711-anti-CD8 (clone 53-6.7) mAbs (BD Biosciences or eBioscience). Cells were washed in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... 2×106 cells/well (6-well plate) were cultured with 1μg/mL of anti-CD3 mAb (553057, BD Biosciences) plus 10 ng/mL of mouse IL-2 (Z02764 ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... and then stained with Alexa Fluor 647 conjugated anti-FOXP3 mAbs (1:100, Clone 259D/C7, BD Biosciences, 560044) to identify CD4+CD25+FOXP3+ Treg cells ...
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bioRxiv - Cancer Biology 2021Quote: ... live CD45+ EPCAM− CD3+ TCRγδ+ cells from five colorectal tumors (MMR-d) were sorted on a FACS Aria III 4L (BD Biosciences). After sorting ...
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bioRxiv - Cancer Biology 2021Quote: ... AKP-Z tumor derived cells were stained using the following antibodies: rat anti-mouse CD45-BV421 (clone 30-F11, 563890, BD Biosciences), rat anti-mouse CD31-BV421 (clone 390 ...
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bioRxiv - Molecular Biology 2021Quote: ... and panned over prewashed 100-mm tissue culture plates coated with CD45 and CD16/32 antibodies (BD Biosciences, San Jose, CA). After incubation for 60 min at 37°C in a 5% CO2 atmosphere to promote adherence of contaminating macrophages and fibroblasts ...
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bioRxiv - Immunology 2022Quote: ... anti-CD45 (clone 30-F11 in PECy7 from eBiosciences Cat# 25-0451-82 or in BV786, from BD Biosciences Cat# 564225); anti-CD45.1 (clone A20 in APC from BioLegend Cat# 110714) ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with fluorochrome-conjugated (FITC, PE, PE-Cy7, allophycocyanin [APC], APC-eFluor 780,) antibodies against mouse CD45 (30-F11; BD Biosciences), Ly6C (AL-21 ...
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bioRxiv - Bioengineering 2019Quote: ... with anti-positive (CD90, CD105, CD44) and negative marker (CD34, CD11b, CD19, CD45, MHC class II) antibodies (Human MSC Analysis Kit, 562245, BD Biosciences), as described in detail in [42] ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were fixed in 4% paraformaldehyde for 30 minutes and stained with PE-conjugated or purified CD45 antibody (eBioscience, 30-F11 or BD Biosciences) to ascertain the generation of haematopoietic progeny ...
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bioRxiv - Cancer Biology 2021Quote: ... and sorted for live macrophages (CD45+CD11b+F4/80+CD3−CD19−Siglecf−Ly6G−Ly6C−7AAD−) by using an Aria II cell sorter (BD Biosciences)
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bioRxiv - Neuroscience 2020Quote: ... followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200, BD Pharmingen; for 30min at 4°C), all in 2% BSA ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with fluorochrome-conjugated (BV421, BV711, FITC, PE, PE-Cy7, allophycocyanin [APC], APC-Cy7) antibodies against mouse CD45 (30-F11; BD Biosciences), Ly6C (AL-21 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells or MNC from CML patients or HD were incubated with the following antibodies: 5 µL CD45-PerCP (2D1, BD Biosciences), 2.5 µL CD34-FITC (AC136 ...
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bioRxiv - Genomics 2021Quote: ... hPBMCs were resuspended in 1x PBS + 2% FBS and stained with a FITC-conjugated Mouse Anti-Human CD45 Antibody (Clone HI30, BD Biosciences) for 25 min on ice ...
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bioRxiv - Microbiology 2020Quote: ... during 15 min at 4 °C in MACS buffer followed by incubation with anti-CD45 1:100 (clone 30F-11, BD biosciences), anti-EpCAM 1:100 (clone G8.8 ...
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bioRxiv - Cancer Biology 2021Quote: ... and Mo-MDSC +M2-like TAMs+ (CD45+CD11b+Ly6c+CD206+) were acquired on a BD LSRII flow cytometer using FACSDiva software (BD Biosciences) for acquisition and compensation and then analyzed using FlowJo software.
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bioRxiv - Cell Biology 2021Quote: ... MPs (CD64+ cells) and FAPs (CD31-/CD45-/a7-/Sca1+ cells) were analysed and sorted using a FACS Aria III cell sorter (BD Biosciences) (gating strategy is shown Figure S3) ...
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bioRxiv - Cancer Biology 2022Quote: ... and basal cell (CD24medCD49fhigh) subpopulations were sorted from the Lin- epithelial cell population (CD45- CD31-Ter119-) using an Aria II cell sorter (BD Bioscience) with over 95% purity.
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bioRxiv - Immunology 2022Quote: ... CD62p PECy7 (Cat. Number 304922, Bio legend), CD45 APCCy7 (Cat. Number 3368516, Bio legend) and CD66b Pacific Blue (Cat. Number 561649, BD Biosciences). About 100 µl of PRP was mixed with 100 µl of antibody cocktail ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... for 20 minutes and then with a blood antibody cocktail for 30 minutes in the dark at 4 degrees: anti-CD45 BB515 (BD Horizon), anti-CD14 PerCP-Cyanine5.5 (eBioscience) ...
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bioRxiv - Cell Biology 2019Quote: Three thousand flow sorted DAPI-CD45-CD31-Epcam+GFP+ cells were cultured with one hundred thousand MLg mouse lung fibroblasts and Matrigel (BD Biosciences) seeded onto Transwell filter inserts (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Infarct zone CD45+CD11b+Ly6G-F4/80+ macrophages were sorted by FACS using a FACS ARIA II flow cytometer (BD Biosciences). Results for the heart digests are expressed as cell number per infarct zone or remote zone ...
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bioRxiv - Physiology 2021Quote: ... Alveolar epithelial cells were identified as CD45-CD31- T1α + and endothelial cells were identified as CD45-CD31+T1α- We analyzed cells by flow cytometry (LSR II, BD Biosciences) and using standard software (FlowJO ...
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bioRxiv - Immunology 2020Quote: ... Intracellular cytokine staining of cells were stained with following antibodies with maintaining supplier’s instructions: V500 anti-mouse CD45 (BD Bioscience, USA), FITC anti-mouse CD4 (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Cancer Biology 2022Quote: ... and engraftment of human cells in the bone marrow and spleen was measured with flow cytometry (CD45-PE-Cy7 antibody, BD Biosciences). Single-cell suspensions were prepared from the bone marrow ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary AML cells were harvested and stained with human CD45 and cells were analyzed by a high-throughput automated plate reader (BD LSRFortessa). Dead cells were excluded using the Near-IR Live/Dead marker (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils (CD45+Ly6ghighLy6clowCD11b+CD3-CD19-F4/80- CD11c-) cells and monocytes (CD45+Ly6chighCD11b+Ly6g-CD3-CD19-F4/80-CD11c-) cells were sorted on a FACSAria Instrument (BD Biosciences) and processed for RNA isolation using the RNeasy Plus Micro Kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4 T cells (live CD19-CD4+CD25-CD45RBhi cells) and colonic monocytes (live CD45+CD19-CD4-CD11b+ cells) were sorted on a FACSAria III (BD biosciences) cell sorter.
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bioRxiv - Immunology 2023Quote: Single-cell suspension from 5 lungs of IAV-infected mice (day 11) were pooled and neutrophil subsets (LiveDead- CD45+ SiglecF- CD11b+ Ly6G+ PD-L1+/-) were sorted on a FACSMelody (BD Biosciences) (purity > 99% ...
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bioRxiv - Immunology 2024Quote: ... Single-cell sorting of lung Vγ4+TCRδ+ from a CD11b- CD11c-CD19-TCRβ- population of CD45+ live lymphocytes were performed using a FACS Aria III cell sorter (BD Bioscience). Cells were sorted directly into 12.5 μl (per well ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed twice in PBS + 2% FBS and stained with mouse anti-human CD45 Pe-Cy5 (BD Bioscience, clone HI30) and mouse anti-human CD107a Alexa Fluor 647 for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... CD45—CD38—CD235a—CD71— non-hematopoietic cells were sorted in DMEM supplemented with 10% FCS using a FACSAria III (BD Biosciences) and BD FACSDiva version 5.0 (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs were stained with a panel of fluorochrome-conjugated antibodies reactive with the following cell surface markers: CD45-APC/Cy7 (clone 30-F11; BD Biosciences), CD3-PE/Cy7 (clone 500A2 ...
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bioRxiv - Neuroscience 2023Quote: ... single viable cells were gated and CD45-O1+ and CD45lowSiglec- H+ cells were collected using a FACSAria III and corresponding software (FACSDiva, v.6; BD Biosciences). Calculation of the number of CD45lowSiglecH+ microglia per brain was performed by extrapolating their frequency to the counted total number of isolated cells ...
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bioRxiv - Genomics 2023Quote: ... for 5 minutes and stained at a dilution of 1:50 with one of two panels of directly conjugated antibodies for 30 minutes at 4 °C: anti-human CD45 (BD 563792), CD204 (Biolegend 371906) ...
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bioRxiv - Immunology 2023Quote: ILC3 cells (CD45+Lin-CD90.2+CD127+KLRG1-) were sorted using the FACSMelody Cell Sorter and the FACSChorus™ software (BD Bioscience). The cells were then incubated in DMEM (high glucose ...