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Citations for Becton, Dickinson and Company :
9651 - 9700 of 9716 citations for Anti p46 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... followed by two washes with the permeabilization buffer before incubation with 4 μg/ml Alexa Fluor® 647 Mouse anti-Phospho-NF-κB p65 (pS529) (BD Biosciences) for 45 min.
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed with PBS+5% FBS and stained with 1:100-dilutions of AF647-conjugated anti-PAX-6 (BD Biosciences; 562249) and PerCP-Cy5.5-conjugated anti-SOX-2 (BD Biosciences ...
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bioRxiv - Bioengineering 2024Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked with 5% skimmed milk in TBS-Tween and incubated with anti-αSyn clone 42 (BD Biosciences, 1:1,000 dilution). In a slot blot ...
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bioRxiv - Immunology 2024Quote: ... The polarization of macrophages from M1 to M2 phenotype was assessed by staining the cells with fluorochrome-conjugated anti-CD206 (BD Biosciences, USA) followed by fixation and permeabilization of the cells using an Intra Prep Permeabilization kit (Beckmann Coulter ...
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bioRxiv - Developmental Biology 2019Quote: Isolated cells from E18.5 Wnt1-Cre;Ift88fl/+;R26mT/mGand Wnt1-Cre;Ift88fl/fl;R26mT/mG embryonic scleras were incubated 45 min with an anti-CD31 Ab (BD Biosciences, 1:500), washed ...
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bioRxiv - Cell Biology 2019Quote: ... for 2 hours at room temperature followed by overnight incubation at 4°C with anti-PECAM-1 (MEC13.3, BD Bioscience #550274, 1:250) diluted in blocking buffer ...
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bioRxiv - Immunology 2022Quote: ... CHO cells expressing CTLA-4 WT or CTLA-4 Del36 were surface CTLA-4 stained on ice with anti-CTLA4-PE (BD Biosciences, clone BNI3) for 60 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was prevented using the ells were stained using the BD Pharmingen Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Clone 2.4G2 ...
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bioRxiv - Neuroscience 2021Quote: ... was proceeded for separation of astrocytes and neurons from co-cultures using the positive selection approach with anti-CD44 (BD-bIoscience, Cat.No. 555478) antibodies [40] ...
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bioRxiv - Immunology 2022Quote: The HLA-A2 status was confirmed by PBMCs staining with 2 μL of anti-HLA-A2 mAb (clone BB7.2) (BD Pharmingen, Franklin Lakes, NJ), at 4°C for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and was added to the media for 3h and 6h followed by flow cytometric analysis as described above using anti-CD11b (WT5. ROU) (BD, Franklin Lakes, NJ).
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bioRxiv - Microbiology 2019Quote: ... Cells were transferred to a 96 well U-bottom plate and Fc receptors on cells were blocked with Rat anti-mouse Fc-Block CD16/CD32-Clone 2.4G2 (BD Pharmigen, San Jose, CA) on ice for 15 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... T cell activation was measured with a PE-conjugated anti-hCD69 on CD3+ gated viable cells by flow cytometry (BD LSRFortessa, BD Biosciences). For T cell proliferation ...
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bioRxiv - Cell Biology 2021Quote: ... cells were counted and resuspended in 500 µL 1% FBS (Cytiva) in PBS with anti-E-cadherin BB700 (BD Biosciences #745965, 1:100), anti-CD44 BV421 (BioLegend #562890 ...
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bioRxiv - Immunology 2020Quote: ... The cell mixture was allowed to incubate for 6 hours in the presence of anti-CD28/Ly29d Abs (BD Biosciences, San Jose, CA), brefeldin A at a final concentration of 10 mg/ml (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in x μL of unlabeled CD16/32 mAb Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™), BD Pharmingen 553141) ...
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bioRxiv - Cancer Biology 2022Quote: ... Human platelets in the obtained blood were identified using APC-labeled polyclonal rabbit anti-human (h) CD41 and/or CD42b (BD Pharmingen, 1:100) and fluorescein isothiocyanate (FITC)-labeled polyclonal rabbit anti-mouse (m ...
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bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
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bioRxiv - Genomics 2022Quote: ... Each surface staining and intracellular staining step was accompanied by a rat FcBlocking step via an anti-CD32(BD Bioscience Clone: D34-485). Events were acquired on a 5-laser custom BD Fortessa X20 analyzer ...
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bioRxiv - Immunology 2024Quote: ... Purified CD4+ cells were cultured in 24-well round-bottom plates (1×106 cells/well) with plate-bound anti-CD3e (2.5 μg/ml, clone 2C11, BD Biosciences, San Diego, CA), soluble anti-CD28 (2 µg/ml ...
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bioRxiv - Cancer Biology 2023Quote: Epithelial cell suspensions were stained for viability using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (Thermo #L23105) and BUV737 Rat Anti-Mouse CD45 Clone 30-F11 (BD Biosciences # 568344) and analyzed using a 5-laser Cytek Aurora.
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bioRxiv - Cell Biology 2023Quote: ... samples were stained with primary anti-TRPM8 (1:100; #ACC-049, Alomone) and CD41a PerCP-Cy™5.5 (1:100, #340930, BD, Franklin Lakes, NJ) antibodies in PBS with 10% normal goat serum for 1hr in the dark and a Hoechst nuclear stain at 1 ug/ml ...
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bioRxiv - Immunology 2023Quote: ... Naïve T cells were resuspended in complete medium (KOHJIN BIO DMEM-H (16003016) + 10% FBS) and activated in the presence of 2 μg/mL of anti-CD3 (BD Pharmingen, REF: 553058) and 2 μg/mL of anti-CD28 (BD Pharmingen ...
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bioRxiv - Immunology 2023Quote: ... cells were washed in perm buffer (PBS, 0.5% BSA, 0.1% Saponin) and stained for IFN-γ (APC rat anti- mouse IFN-γ; 1:100; BD Biosciences, Cat. 554413) and TNF (PE rat anti-mouse TNF alpha ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then suspended in 0.5% Tween 20 (vol/vol) plus 1% BSA/PBS and incubated for 30 minutes at room temperature with anti-BrdU FITC (BD Biosciences, Cat. #347583) at a concentration of 0.5 µg/106 cells ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667, 1:100 in 1X BD Perm/wash solution) or anti-BST2 (Proteintech #13560-1-AP ...
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bioRxiv - Genomics 2023Quote: ... and half of the remaining chromatin was rotated overnight at 4°C with 4.5 µg of anti-p53 (BD Biosciences #554293, Lot #9046678). The other half of the remaining chromatin was also rotated overnight as a no-antibody control ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Genomics 2024Quote: ... 2.5 x 105 cells were stained for 75 minutes at room temperature with PE Mouse anti-PDX1 Clone-658A5 (BD Biosciences; 1:10) and Alexa Fluor® 647 Mouse anti-NKX6.1 Clone R11-560 (BD Bioscience ...
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bioRxiv - Cell Biology 2024Quote: ... The single cell suspension obtained from human muscle was stained with a cocktail containing PE-Cy5 anti-human CD45 (BD Pharmingen, cat#555484), Percp-Cy5.5 anti-human CD31 (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... slides were processed as described previously for section immunofluorescent staining for detection of mouse IgG2a anti-E-cadherin (1:500, BD Biosciences, Cat# 610181) and imaged on a Leica SP5 upright confocal ...
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bioRxiv - Immunology 2024Quote: ... cells were stimulated for 1h in TCM with peptides (EO2316, EO2317, EO2318, IL13RA2, BIRC5, and FOXM1 at 10µg/mL each) along with anti-CD107a (BD Biosciences, FITC, clone H4A3) to capture degranulation ...
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bioRxiv - Immunology 2024Quote: ... CD4+CD25- T effector cells were isolated from the Treg-depleted fraction of splenocytes using Anti-Mouse CD4 Magnetic Particles – DM (BD Biosciences, CA, USA). DCs were isolated from total splenocytes using CD11c microbeads according to the manufacturer’s protocols (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: Purified CD4+ T cells or naive CD4+ T cells were stimulated with beads (Dynabeads M-450 Epoxy, ThermoFischer Scientific, Waltham, USA) coated with anti-CD3 (7.5%, clone 145-2C11, BD Biosciences, San Diego, USA) / anti-CD28 (7.5% ...
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bioRxiv - Developmental Biology 2021Quote: ... were plated in buffer (PBS with 0.5% bovine serum albumin and 1 mM EDTA) and non-specific Fc binding was blocked via incubation with mouse anti-rat CD32 (BD Biosciences #550271; 1:200) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2019Quote: ... Intracellular cytokine staining was performed according to the manufacturer’s protocol (Cytofix/Cytoperm buffer set from BD with PE-Cy7-conjugated anti-CD4 (RM4-5), eFluor 450-conjugated anti-TCR-β (H57-597) ...
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bioRxiv - Bioengineering 2020Quote: ... Coated beads were then incubated with anti-human CD63 (eBioscience, San Diego, CA) and analysed with a BD AccuriTM C6 (BD Biosciences, San Jose, CA).
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bioRxiv - Neuroscience 2020Quote: ... The transfer of the retinal suspension onto panning plates bound with monoclonal mouse anti-SSEA-1 (CD15)(MC480 clone) (#BD560079; BD Pharmingen, San Jose, CA) and monoclonal mouse anti-HNK-1/N-CAM (CD57)(VC1.1 clone ...
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bioRxiv - Genetics 2020Quote: ... Cells were stimulated at 1×106 cells/ml with 10 μg/mL soluble Leishmania antigen (SLA) plus 1 μg/mL purified NA/LE anti-human CD28 (clone CD28.2, BD Biosciences, San Jose, CA, USA) at 37°C / 5% CO2 for 15 h and subsequently incubated in the presence of brefeldin A (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... the cells were co-cultured in R10 medium alone or in presence of K562 cells for 2 hours in the presence of anti-human CD107a (FITC or Brilliant Violet 421, H4A3, BD Biosciences, San Jose, Calif.).
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bioRxiv - Cell Biology 2020Quote: ... re-suspended in αMEM (with 10% FBS and 1% anti-mycotic) and then processed on an Aria II or Influx flow cytometer (BD Biosciences, San Jose, CA) to separate tdTomato-expressing (tdTom+ ...
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bioRxiv - Immunology 2020Quote: IgG subclasses were measured from day 21 sera using the same ELISA protocol with the following secondary Abs: goat anti-mouse IgG1-HRP (BD Biosciences, San Jose, California), goat anti-mouse IgG2b-HRP (1:1000 ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... CD45+ cells were depleted with mouse CD45 microbeads from Miltenyi Biotech(Auburn, CA) and stained with anti-Ter119-PE (BD Biosciences, San Jose, CA), anti-CD45-FITC ...
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bioRxiv - Bioengineering 2022Quote: ... Macrophages were characterized for presence and absence of canonical markers using flow cytometry PE-Cy5 mouse anti-rat CD45 (BD Biosciences, Mississauga, ON, Canada), BV786 mouse anti-rat CD11b/c (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and F15 NOD Nfκb1 heterozygote were suspended in biotin-free RPMI containing 0.1% azide and 3% FCS and surface stained in 96-well plates with the 10–3.6 PE (anti-I-Ag7) (BD PharMingen, Franklin Lakes, NJ, USA), which is class II major histocompatibility complex (MHC ...
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bioRxiv - Cell Biology 2024Quote: ... Cells from the stroma were sorted gating on single GFP-positive cells from PdgfrαH2Be-GFP mice or using anti-CD45-PE/Cy7 (BD Pharmingen, clone 30-F11, #552848) antibodies ...
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bioRxiv - Cancer Biology 2023Quote: CD4+ and CD8+ cells were isolated from spleens of RD and IF tumor bearing mice using BD-IMAG anti-mouse CD4 and CD8 magnetic particles (BD Biosciences, San Jose, CA) and were plated at a density of 7 x105 cells/well in cell-tak coated XFe 96 cell plates ...