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Citations for Becton, Dickinson and Company :
9601 - 9650 of 9716 citations for Anti p46 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Antibody staining for Cxcr4 was performed for 20 mins on ice using 1:100 APC-conjugated Rat anti-Mouse CD184/Cxcr4 (BD Pharmingen) in FC buffer and cells were then washed twice in FC buffer and resuspended in 1 μg/ml DAPI in FC buffer at ∼1-1.5 x 1E7 cells/ml to label dead cells and run the suspension through a cell strainer ...
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bioRxiv - Immunology 2024Quote: T cells were restimulated with 3 μg/ml plate-bound anti-CD3 and 2 μg/ml soluble anti-CD28 or PMA/Ionomycin in the presence of GolgiPlug protein transport inhibitor (BD Biosciences) for 6 hours ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Fixed cells were stained for 30 min at 4° C with PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... BSA was removed and the desired mouse primary antibody was added in Antibody solution (0.5%BSA/0.01% Triton X-100/PBS) at the indicated dilutions: Anti-β-Catenin Clone 14/Beta-Catenin (BD Biosciences, 1:1000), anti-Lamin A (Abcam ...
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bioRxiv - Pathology 2020Quote: ... Approximately 9 mL of cord blood from gravidas chosen for this study was collected into anti-coagulant K2-EDTA tubes (BD Vacutainer 366643) containing a proprietary preservative.
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bioRxiv - Cancer Biology 2020Quote: ... Then cells were blocked by PBS plus 5% BSA and later incubated with FITC Anti-Human CD24 (BD Biosciences, San Jose, CA), and PE Anti-Human CD44 (BD Biosciences) ...
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bioRxiv - Genomics 2021Quote: ... anti-CD4 (L200: AmCyan; BD Biosciences, Custom Bulk 658025, BV510; BD Biosciences, Custom Bulk 624340 and BUV395; BD Biosciences, Custom Bulk 624165), anti-CD8a (SK1 ...
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... Lactate excretion in primary mouse T cells was measured using a YSI-2900 Biochemistry Analyzer after 24 hours of stimulation using anti-CD3e (BD Biosciences 553057) and anti-CD28 (BD Biosciences 553294 ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for 1 hour at 4 °C with 2 µl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Cancer Biology 2020Quote: ... at a density of 1 million cells per 100 μL and stained with anti-mouse CD16/CD32 Fc Block™ (BD Pharmigen) for 10 min ...
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bioRxiv - Bioengineering 2021Quote: ... To assess activation marker status T cells were stimulated with 0.1 μg/mL of D117C or PIGF on collagen coated plates for 16-20 hr and surface-stained with anti-human CD3 BUV395 (BD clone SK7), CD4 BV785 (Biolegend clone RPA-T4) ...
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bioRxiv - Biochemistry 2022Quote: ... resuspended in 50ul FACS buffer containing 0.5 μg anti-mouse CD16/CD32 mAb 2.4G2 (Mouse BD Fc Block, BD Pharmingen, San Diego, California), and incubated for 15 minutes 4° C ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in blocking solution of 1% normal horse serum in PBS with 0.3% Triton X-100 and incubated overnight at 37°C as follows: mouse (IgG1) anti-CtBP2 (C-terminal Binding Protein) 1:200 (#612044, BD Transduction Labs) targeting pre-synaptic ribbons ...
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bioRxiv - Cell Biology 2021Quote: γ-H2AX antibodies used were p-Histone H2A.X S139 (20E3) Rabbit mAb (Cell Signalling Technology, #9718) and Alexa Fluor 647 Mouse anti-H2AX (pS139) Clone N1-431 (BD Pharmingen, 560447).
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bioRxiv - Cancer Biology 2021Quote: ... and stained for 1 hour at 4 °C with 2 μl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and incubated in DMEM media containing 1% FBS and 1% BSA with Alexa Fluor® 647 Mouse Anti-Human LAMP1 Clone H4A3 (BD Biosciences) for 1h at 4°C with intermittent mixing ...
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bioRxiv - Cancer Biology 2019Quote: 5×105 human CD14+ cells were isolated from peripheral blood mononuclear cells (PBMC) using anti-CD14 beads (BD Biosciences, San Jose, CA) with a typical yield of ≥ 70% recovery and ≥ 90% purity ...
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bioRxiv - Microbiology 2019Quote: ... for 1 h or 2 h and then subsequently incubated for 30 min on ice with an APC-conjugated rat anti-mouse IgG (BD Biosciences, CA). Stained cells were analyzed using a BD AccuriTM C6 Plus with a Sampler flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... slides were incubated for 50 min at 25 °C with different combinations of the following anti-mouse Abs (Thermo Fisher Scientific, Biolegend, BD Biosciences): Alexa Fluor 488-labeled anti-CD3 (HM3420 ...
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bioRxiv - Immunology 2019Quote: ... 1 µL of mAbs were also added to one drop of compensation beads (Anti-Rat Ig, κ/Negative Control Compensation Particles Set (BD Biosciences)) to prepare single-stain controls for compensation ...
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bioRxiv - Immunology 2019Quote: ... Immune cells were purified by FACS isolation of CD45 positive cells using APC Mouse Anti-Human CD45 (BD Pharmingen, clone: HI30 (RUO), American ...
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bioRxiv - Biochemistry 2020Quote: ... and CD81 antibodies (with no beads) were added to the solution containing the EVs (PE Mouse Anti-Human CD81 Clone JS-81, BD Pharmingen™). After 1 h of incubation ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then blocked with 10% goat serum in staining buffer (1% bovine serum albumin (BSA) plus 0.05% Tween 20 in PBS) for one hour prior to staining with mouse anti-GM130 (BD Transduction Laboratories 610822) or mouse anti-Calreticulin (abcam ab22683 ...
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bioRxiv - Immunology 2020Quote: ... Single cell suspensions from mouse tissues or tumors were incubated for 15 minutes at ambient room temperature with rat anti-mouse Fc Block™ (BD Pharmingen). Cells were then incubated with primary antibodies for 30 minutes at 4ºC in the dark ...
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bioRxiv - Immunology 2019Quote: ... Antibodies used for the blockade of B7-1- and B7-2-dependent CD28 costimulation were anti-mouse CD80 Clone 16-10-A1 (BD Pharmingen #553736) and anti-CD86 Clone GL1 (BD Pharmingen #553689) ...
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bioRxiv - Immunology 2021Quote: ... and viability dye (eBioscience™ Fixable Viability Dye eFluor™ 780) followed by Foxp3 staining with anti-FoxP3 (259D/C7) using Human FoxP3 buffer set (BD biosciences) according to the Manufacturer’s recommendations.
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bioRxiv - Cell Biology 2020Quote: ... The following antiboodies were used in combination for the depletion of lineage-positive cells: anti-CD4 (RM4-5, BD Biosciences, 1:100), -CD8 (53-6.72 ...
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bioRxiv - Cell Biology 2021Quote: Cells were treated with 25uM BrdU for 1 hour and BrdU staining detected using FITC Mouse Anti-BrdU Set (BD Biosciences, 556028). Cells were resuspended in 10μg/ml 7-Amino-Actinomycin D (7-AAD ...
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bioRxiv - Immunology 2020Quote: ... a panel of four fluorescent markers was employed including fluorescein isothiocyanate (FITC) mouse anti-human CD40 (BD Biosciences; San Jose, CA, USA), phycoerythrin (PE ...
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bioRxiv - Immunology 2020Quote: ... and BV421 rat anti-mouse TNF-α BD Biosciences in a total of 100 μL of 1× Perm/Wash Buffer (BD Biosciences). After two washes ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and centrifuged at 1000rpm for 5-10min and stained with APC-Cy™7 Rat Anti-Mouse CD3 (clone: 17A2, Cat # 560590, BD Biosciences), FITC Rat Anti-Mouse CD4 (Clone ...
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bioRxiv - Immunology 2021Quote: ... 50 U/ml Penicillin and 50 ug/ml Streptomycin) supplemented with 100 U/ml hIL-2 and 2 ug/ml anti-CD28 (BD Pharmingen, 553294). Two days after the primary stimulation ...
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bioRxiv - Immunology 2021Quote: ... before resuspending in 25 μl of 0.2µm-filtered 1% BSA/PBS containing monoclonal FITC-anti-mouse IgA (BD Pharmingen, 10 µg/ml) or Brilliant violet 421-anti-IgA (BD Pharmingen ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell pellet was suspended with FACS medium and incubated with 5 μl of Alexa Fluor 488-conjugated anti-alkaline phosphatase (BD Pharmingen, 561495) and 5 μl of APC-conjugated anti-c-KIT (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... samples were stained for LSKCD48CD150 first and further stained for the intracellular anti-Ki-67 Alexa Fluor® 647 (Clone B56, BD Pharmingen) and DAPI using the FIX & PERM™ Cell Permeabilization Kit from Thermo Fisher (cat# GAS003) ...
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bioRxiv - Immunology 2022Quote: ... 1 μg of purified rat anti-mouse CD16/CD32 was added to each pool (Mouse BD Fc Block, BD Biosciences, cat. 55142) and incubated for 30 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 1 μg of purified rat anti-mouse CD16/CD32 was added to each pool (Mouse BD Fc Block, BD Biosciences, cat. 55142) and incubated for 30 minutes at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then washed with PBS+5% FBS and stained with 1:100-dilutions of AF647-conjugated anti-PAX-6 (BD Biosciences; 562249) and PerCP-Cy5.5-conjugated anti-SOX-2 (BD Biosciences ...
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bioRxiv - Synthetic Biology 2023Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... cells were counted using a hemacytometer and then treated with an Fc blocking buffer containing anti-Cd16/32 (BD Biosciences, 1:500) for 20 minutes at 4 degrees Celsius before a 30 minute incubation with antibody master mixes for the myeloid or lymphoid panels at 4 degrees Celsius ...
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bioRxiv - Cell Biology 2024Quote: For single-cell RNA sequencing experiments cells were washed and stained with anti-CD45-PE/Cy7 (BD Pharmingen, clone 30-F11, #552848) and SYTOX™ Green Ready Flow™ Reagent (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 5X106 cells per mL were washed with cold PBS and blocked in PBS with Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Biosciences) for 15 min at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... rat monoclonal anti-mouse/rat FOXP3 (clone FJK-16s; eBioscience #14-5773) and rat monoclonal anti-mouse B220/CD45R (clone RA3-682; BD Pharmingen #550286). Each stain was evaluated based on two to three complete cross sections from 4-5 grafts per group ...
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bioRxiv - Biochemistry 2023Quote: ... cells were processed as for CDC45 but without the methanol fixation step and with a 1:1000 dilution of mouse monoclonal anti-MCM2 (BD Biosciences, 610701) for 14 hours at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated in Tfh conditioning media (RPMI complete media with 10% fetal bovine serum, 10μg/ml anti-IL4 (BD Biosciences, clone 11B11), 10μg/ml anti-IFNγ (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: The purity of each NK population was checked before and after cell sorting and routinely by flow cytometry with anti-human CD16-BV421 (clone 3G8, BD Pharmagen, France), anti-human CD56-PeCy7 (clone B159 ...
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bioRxiv - Developmental Biology 2023Quote: ... were stained with Viability Dye (VD) eFluor 780 (eBioscience #65-0865-14) and with anti-CD15-Alexa Fluor 647 (BD Bioscience #560120) and CD15+ ...
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bioRxiv - Immunology 2023Quote: ... and co-cultured with human CD8+ T cells at 1:1 E:T ratio in the presence of anti-CD107a-APC mAb (BD Biosciences, CA, USA). After 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked with 5% skimmed milk in TBS-Tween and incubated with anti-αSyn clone 42 (BD Biosciences, 1:1,000 dilution). In a slot blot ...