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Citations for Becton, Dickinson and Company :
901 - 950 of 1012 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... A primary antibody dilution was prepared in the blocking solution with the antibodies diluted 1:200 for HIF1α (BD Bioscience, 610958), 1:50 for phosphor-S6 (CST ...
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bioRxiv - Bioengineering 2022Quote: The samples were diluted in proportion of 0.5% with PBS and 10 μL of the diluted solution were sampled in every measurement using a flow cytometer (LSRFortessa4, BD, USA). GFP were excited at 488 nm ...
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bioRxiv - Microbiology 2020Quote: ... LB-Miller agar solutions (molten) were prepared by combining LB-Miller medium with agar (final concentration of 1.5% w/v) (BD Difco™) and autoclaving ...
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bioRxiv - Microbiology 2020Quote: ... these cells were stimulated with a 10 μg/ml solution of CD28 and CD49d co-stimulatory antibodies (both from BD Biosciences) and 1ug/ml of 15-mer overlapping peptide pools spanning either the SARS-CoV-2 spike (S) ...
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bioRxiv - Cancer Biology 2021Quote: 3D-grown MCF-10A and MCF-10A MYC-ER cells were washed with PBS (1X) and the Matrigel was dissolved by incubating slides at 4°C in Cell Recovery Solution (BD Biosciences). Cells were lysed in Laemmli buffer (50 mM Tris-HCl pH 6.2 ...
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bioRxiv - Cancer Biology 2021Quote: 3D-grown MCF-10A cells were washed with PBS (1X) and the Matrigel was dissolved by incubating slides at 4°C in Cell Recovery Solution (BD Biosciences). 2D-grown HEK293T ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was performed after treatment with Fixation/Permeabilization solution and in the presence of Perm/Wash buffer (Cytofix/ Cytoperm kit, BD Biosciences) using anti-mouse IFN-γ FITC ...
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bioRxiv - Microbiology 2021Quote: Mouse spleens were collected in ice-cold storage solution (PBS 2% FBS) and single-cell suspensions prepared using cell strainers (BD-Falcon). Cells were washed upon red blood cells lysis (150 mM NH4Cl ...
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bioRxiv - Bioengineering 2021Quote: ... We incubated the ECs in a 1:500 primary antibody solution containing Mouse anti-CD144 (VE-Cad, BD Pharmingen Cat. #55561) in ADB overnight at 4 oC ...
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bioRxiv - Immunology 2020Quote: ... mouse anti human ICAM-1 (CD54) and mouse anti human VCAM-1 (CD106) antibodies and fixed with FACS lysing solution (BD Bioscience). Surface levels were determined by flow cytometry (Gallios ...
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bioRxiv - Genetics 2021Quote: ... at least 2x106 iPSC-PPC cells were fixed and permeabilized using the Fixation/Permeabilization Solution Kit with BD GolgiStopTM (BD Biosciences) following manufacturer recommendations ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... activation of T-cells was measured by flow cytometry using the anti-human CD4 (FITC)/CD69 (PE)/CD3 (PerCP) antibody staining solution (BD Biosciences) in combination with anti CD25 (APC ...
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bioRxiv - Immunology 2021Quote: ... 4 and 6 hours and cytokines detected by intracellular cytokine staining (see Table S2 for antibodies list) using Fixation/Permeabilization Solution Kit (BD Biosciences), unstimulated cells were used as a control.
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bioRxiv - Immunology 2021Quote: ... The cells were then washed with FACS buffer and fixed and permeabilized using Cytofix/Cytoperm Solution (BD Biosciences, San Jose, CA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Samples were then fixed with 1X BD FACS lysing solution before acquiring data on a FACSSymphony A5 flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Spike -specific T cells were measured by surface staining followed by fixation and permeabilization using BD fixation/permeabilization solution kit (BD Biosciences,) and intracellular cytokine staining (table S1) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice in wash buffer and residual red blood cells were lysed using BD FACS Lysing Solution (BD Biosciences) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... samples were incubated for a further 15 minutes at room temperature with 500 μl of 1X BD Lysis solution (BD Biosciences) to lyse red blood cells ...
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bioRxiv - Immunology 2022Quote: ... and cells were fixed and permeabilized at room temperature for 15 min with Fixation/Permeabilization Solution Kit (BD Biosciences; cat. #554714). For each sample a minimum of 1.2×105 stopping gate events (live CD3+ T-cells ...
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bioRxiv - Genomics 2022Quote: ... 106 cells were resuspended in PBS supplemented with 2% fetal bovine serum and incubated with human Fc blocking solution (BD Biosciences) for 10 minutes at 4°C ...
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bioRxiv - Genomics 2024Quote: ... at least 2 x 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2023Quote: ... The flowers and the fixing solution were removed and replaced with 1 µg/mL DAPI (4′,6-diamidino-2-phenylindole, BD Biosciences) diluted in pollen isolation buffer before visualizing under a fluorescence microscope using a 420 nm filter (Zeiss LSM880 Inverted Confocal Microscope) ...
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bioRxiv - Immunology 2024Quote: ... PMA [1μg/ml] and ionomycin [0,5μg/ml] with Golgi Plug [1:1000] and Golgi Stop [1:1000] solutions (Cytofix/Cytoperm TM Plus, BD Bioscience, ref: 555028) for 4-5h at 37°C to block Golgi’s exocytosis ...
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bioRxiv - Biophysics 2023Quote: ... we let bead-PBS solution flow through a set of strainers with mesh sizes of 70 μm (Cell strainer 70 μm Nylon, BD Falcon) and 40 μm (Cell strainer 40 μm Nylon ...
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bioRxiv - Cancer Biology 2023Quote: ... or ASC52telo cells (ATCC SCRC-4000) were suspended in 5%FBS/PBS solution and the following antibodies were added: APC-CD14 (BD 561708), APC-CD19 (BD 561742) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed with staining buffer, fixed, and permeabilized (20 min, 4°C, dark) using Fixation and Permeabilization Solution (BD Biosciences). Cells were then stained with S100A9 (Clone ...
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bioRxiv - Cancer Biology 2023Quote: ... admixed with an equal number of HDFs expressing either S301D mutant or wildtype lamin A/C were re-suspended in 70 µl of Matrigel solution (BD Bioscience) and injected intradermally into the left and right side of the mouse back ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, for the RBD ELISA, and BD OptEIA Substrate Solution ...
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bioRxiv - Cell Biology 2023Quote: ... 10-μM bromodeoxyuridine (BrdU) solution was added 4 h before the end of the experiment [fluorescein isothiocyanate (FITC) BrdU Flow Kit (BD #559619)] according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were pre-incubated for 15 minutes in a staining solution (PBS+/+, 2% FBS) containing 1 μg/mL Fc block (BD Biosciences), followed by an incubation with the antibodies for 45 minutes (Table S1) ...
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bioRxiv - Developmental Biology 2023Quote: ... and then resuspended in 100 μL Annexin V binding buffer with either (i) FITC-Annexin V conjugated antibody (1:33 dilution in TBST) and BD propidium iodide staining solution (BD Pharmingen) or (ii ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then stained with live/dead and cell surface markers in MACS solution (Miltenyi) supplemented with 2% BSA (Miltenyi) prior to permeabilization with Cytofix/Cytoperm (BD Biosciences) and staining with intracellular markers in Perm/Wash (BD Biosciences) ...
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bioRxiv - Bioengineering 2023Quote: ... iPSC were detached and singularised using EDTA solution (0.5 M EDTA/PBS) and replated on Matrigel (1:120, BD Bioscience, #354230) at a density of 8,000-12,000 cells/cm2 ...
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bioRxiv - Systems Biology 2023Quote: ... A PDMS chamber was put on the mold and a solution of collagen rat tail I (5 mg/ml, BD Biosciences) was injected and allowed to polymerize at 37 °C for 1.5 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... and total DNA (7-AAD 1:50 solution dilution) and analyzed by flow cytometer in a BD LSRFortessa cell analyzer (BD Biosciences) using 488 nm and 640 nm lasers ...
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bioRxiv - Cancer Biology 2023Quote: ... After washing the slides were blocked for 30 min in Superblock solution following the incubation with the primary antibodies mouse anti-BrdU/IdU (1:100, BD Bioscscience) and rat anti-BRDU/CIdU (1:200 ...
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bioRxiv - Genetics 2023Quote: ... 1.106 cells were incubated 20 min at 37°C in 100 μL solution BD cytofix/ cytoperm (BD PharmingenTM BrdU Flow Kit, 552598, BD Bioscience), washed with PBS and re-suspended in 700 µl of staining buffer (PBS with 3% SVF) ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with fluorochrome-conjugated monoclonal antibodies (mAbs) diluted at 1:200 into a 1:3 solution of Brilliant Staining Buffer (BD 563794): Cell Staining Buffer ...
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bioRxiv - Immunology 2023Quote: ... samples were incubated for a further 15 minutes at room temperature with 500 μl of 1X BD Lysis solution (BD Biosciences) to lyse red blood cells ...
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bioRxiv - Immunology 2023Quote: ... solution for 20 minutes followed by 20 minutes of antibody cross linking on ice with 2 µg/mL goat-anti mouse solution (BD, #53998). T cells were then activated via an incubation period at 37 °C in FACS buffer (PBS with 2% FBS and 2 mM EDTA ...
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bioRxiv - Microbiology 2023Quote: ... Afterwards cells were washed twice with PBS and permeabilized 20 minutes with 200µl BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (BD Biosciences)at RT ...
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bioRxiv - Cell Biology 2023Quote: Both WT and CD13KO mice of both genders were intraperitoneally injected with 1 mL 4% Brewers Thioglycollate (TG) solution (BD; 211716). After 48 hours mice were then euthanized via CO2 and cervical dislocation and injected with 10 mL cold PBS into the peritoneal cavity to collect peritoneal macrophages ...
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bioRxiv - Genomics 2023Quote: ... at least 2 × 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... Samples for flow cytometry were fixed in 2% paraformaldehyde solution in PBS and analyzed using a LSRII flow cytometer (BD Biosciences) and FlowJo software (Tree Star ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilized using a BD Cytofix/ Cytoperm Fixation/ Permeabilization Solution kit (Cat# 554714 BD Biosciences, Franklin Lakes NJ) followed by intracellular staining with anti-IFNγ ...
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bioRxiv - Bioengineering 2024Quote: Bacteria were stored for long term at -80 °C in standard glycerol solution and were incubated overnight at 37 °C in BD BACTEC Plus Aerobic medium (BD, USA), referred to as Blood Culture Medium (BCM) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were resuspended with 200 μL of the working solution and immediately analyzed using a BD FACSCalibur Flow Cytometer (BD Biosciences). Twenty thousand events were then analyzed by flow cytometer on the fluorochrome PI channel (488Lnm excitation laser and long pass filter 556 and band pass filter 616/23 for emission) ...
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bioRxiv - Immunology 2024Quote: ... CFSE-labelled cells were boosted with HIV-1-Gag or vaccine-specific peptide pools in the presence of Brefeldin A solution (BD, Biosciences) and Monensin solution (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... Organoids were cultured for 14 d for counts to monitor formation and were harvested from Matrigel using cell recovery solution (BD Biosciences). For human epithelial cell cultures ...
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bioRxiv - Immunology 2024Quote: ... 0.5mL heparinized whole blood was collected using cardiac puncture from EAE (D7, D12) and control mice and subjected to RBC lysis using RBC lysis solution (555899, BD Biosciences) before proceeding with flow cytometry ...