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Citations for Becton, Dickinson and Company :
851 - 900 of 1012 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... DNA denaturation was achieved with 0.5% BSA and 2M HCl after which cells were incubated in 1:2000 solution of anti-BrdU (BD Pharmingen) for 1h at RT ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were resuspended with a 0.5% BSA/PBS solution in a FACS tube and analyzed with a FACSAria IIIu Cell Sorter (BD Biosciences), recording 20,000 events for each sample ...
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bioRxiv - Biochemistry 2020Quote: ... The resins were pre-treated with yeast extract solution (0.1 g/100 ml H2O for 1 hour; BD Biosciences, NJ) to avoid non-specific binding of EVs to resins ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were further incubated for 1 hr in solution containing goat anti-rabbit antibody conjugated with FITC (BD Biosciences, USA). All steps were carried out at room temperature ...
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bioRxiv - Genetics 2022Quote: ... the majority of each sample was then resuspended and fixed in Cytofix/Cytoperm solution (100 μL/sample, BD Bioscience, 554722) following manufacturer’s instructions and washed in Perm/Wash buffer (2 mL/sample ...
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bioRxiv - Immunology 2022Quote: ... Intracellular cytokine staining was performed by activating cells for 4-5h at 37°C with the Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) and subsequent fixing and staining per manufacturer’s instructions using the BD Fixation/Permeabilization Solution Kit (BD Biosciences). For intranuclear transcription factor staining ...
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bioRxiv - Pathology 2021Quote: ... Whole blood samples were then lysed and fixed in 2 ml of FACS Lysing Solution (BD Biosciences, San Jose, CA) for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... single cells were washed three times in FACS buffer (0.1% (w/v) BSA in DPBS) and then fixed and permeabilized with Cytofix/Cytoperm Fixation/Permeabilization Solution (BD Biosciences) for 20 min at 4 °C ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... The 100 μl of the resuspended solution was incubated with 5 µl of FITC Annexin V (BD Pharmingen™, 560931) in FACS tube for 15 min at RT (25°C ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA, and BD OptEIA Substrate Solution ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA,and BD OptEIA Substrate Solution ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were stained with hypotonic fluorochromic solution (HFS) and parameters were acquired by flow cytometry (FACSCan®, BD Biosciences, USA) and analyzed in the software FlowJoX.0.7® (Tree Star ...
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bioRxiv - Immunology 2020Quote: ... antibodies were used for intra-cellular staining by using the Fixation/Permeabilization Solution Kit (BD Cytofix/Cytoperm™, BD Biosciences). For in vitro intracellular cytokine staining ...
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bioRxiv - Immunology 2020Quote: ... antibodies were used for intra-cellular staining by using the Fixation/Permeabilization Solution Kit (BD Cytofix/Cytoperm™, BD Biosciences). For in vitro intracellular cytokine staining ...
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bioRxiv - Cell Biology 2020Quote: ... IdU and CldU were then detected with the following primary antibodies in blocking solution for 1 hour at room temperature: mouse anti-BrdU (B44) (1:25) (BD) for IdU and rat anti-BrdU [Bu1/75 (ICR1)] (1:100 ...
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bioRxiv - Plant Biology 2020Quote: In vitro mycelium diameter measurements were performed by depositing a 5 μl droplet of a 1.5 × 105 spores ml−1 solution onto 1.5 % granulated agar (BD Difco™) plates supplemented with homogenates of WL- or WL+FR-treated leaves (1 gFW ml−1) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-F4/80-PE clone REA126 and human anti-CD14-FITC from Miltenyi Biotech and 7-AAD staining solution from BD Pharmingen ...
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bioRxiv - Immunology 2021Quote: ... 20µL of the resulting solution was subsequently spread onto a TSB agar plate (tryptic soy broth [TSB] plus 1.5% Bacto agar; BD Biosciences) and incubated for 16 hours at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Spleen and lung cells that were stimulated overnight with peptide pools (as described under ex-vivo stimulation and intracellular staining) were fixed and permeabilized with Cytofix/Perm solution (BD) and then stained for intracellular cytokines ...
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bioRxiv - Immunology 2022Quote: ... Cells were allowed to migrate for 1hr at 37°C and subsequently fixed with 150 µl of fixation solution (CellFIX, BD). Number of migrated neutrophils was measured at a BD FACSCanto II for 30 sec and separated from eosinophils based on their different auto-fluorescence in the V450 channel.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were fixed with Crystal violet solution (3gm in 5% Methanol: 5% Isopropanol) (BD BBL Gram Crystal Violet, Cat # 212525) and counted manually using ImageJ ...
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bioRxiv - Immunology 2022Quote: ... Red blood cells in the whole blood samples were lysed using a commercial BD FACSTM Lysing Solution (349202, BD Biosciences). The samples were run/acquired on a FACScanto II (BD Biosciences) ...
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bioRxiv - Cell Biology 2024Quote: ... the coverslips were decorated with the following primary antibodies diluted in a 3% BSA solution for 1h30min at room temperature: mouse monoclonal anti-TIM23 (BD Transduction Laboratories ...
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bioRxiv - Systems Biology 2023Quote: ... 4×106 cells per sample were incubated in blocking solution containing 5% fetal bovine serum and 2% FcBlock (BD Biosciences) in PBS ...
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bioRxiv - Immunology 2023Quote: ... The resulting single cell suspensions were filtered through a 70µm nylon filter and treated with BD Pharm Lyse lysing solution (BD Biosciences) to eliminate erythrocytes ...
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bioRxiv - Cancer Biology 2023Quote: ... single-cell suspensions of BILs (0.5–1×106 cells) were stained with Fixable Viability Stain 780 solution (BD Biosciences, 565388) to discriminate live and dead cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was Fc blocked before staining using 50 µL of 2X FC Block Solution (FACS Buffer, 1:125 dilution of anti-CD16/CD32 (BD) and incubated for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Subsequently cells were fixed and permeabilized using the BD Cytofix/Cytoperm solution as recommended by the manufacturer (BD Bioscience, USA). Afterwards cells were washed and incubated with the antibody staining cocktails for 1hour at RT in the dark.
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bioRxiv - Immunology 2023Quote: ... The protein binding capacity of the well surface was saturated with 300 μL blocking solution (PBS supplemented with 2% skim milk powder (BD) and 0.1% Tween 20 ...
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bioRxiv - Cell Biology 2023Quote: Cells were analyzed for cell cycle phase using a ClickIT EdU Alexa Fluor 647 kit (Fisher) and FxCycle PI/RNase Staining Solution (Fisher) followed by flow cytometry on an LSR-II (BD) and analysis using FlowJo.
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bioRxiv - Genetics 2023Quote: ... 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution: FITC-conjugated lineage cocktail with antibodies against CD4 (clone H129.19, BD Pharmingen), CD3e (clone 145-2C11 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated for 30 minutes at room temperature or overnight at 4°C with the indicated primary antibodies diluted 1:100-1:200 in blocking solution (mouse IgG1 anti-MIC60 [110329; Abcam], mouse IgG2a anti-TIMM23 [611222; BD Biosciences] ...
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bioRxiv - Microbiology 2024Quote: ... and a small portion of the frozen glycerol solution was taken from the tubes and plated on Vibrio-selective MTCBS plates (BD Difco TCBS Agar 265020 ...
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bioRxiv - Biochemistry 2024Quote: ... The 1 mM Rhobo6 solution was then injected retro-orbitally using a 0.5 mL tuberculin syringe with 27G needle (BD, 305620). Mice were allowed to recover in their cage over 30 mins then euthanized by cervical dislocation ...
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bioRxiv - Cancer Biology 2024Quote: ... Wells were then incubated with an HRP-coupled CD63-specific antibody (1µg/ml) and developed with OptEIA TMB substrate solution (BD Biosciences), stopped with 1 M H2SO4 and read immediately on a plate reader at 450 nm.
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bioRxiv - Immunology 2024Quote: ... cells were resuspended in 200 μL of FCM buffer including 20 μL 7-amino-actinomycin D (7-AAD) viability staining solution (BD) and incubated for 10-30 minutes before acquisition ...
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bioRxiv - Immunology 2024Quote: ... samples were washed once with FACS buffer and fixed and permeabilized by adding 500 μL fixation/permeabilization solution (BD Biosciences). After 20 minutes of incubation ...
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bioRxiv - Microbiology 2024Quote: ... or bile-dilutions in Cullen–Haiser Gut (CHG) media (brain heart infusion (BHI, Bacto) supplemented with 1% BBL vitamin K1-hemin solution (BD), 1% trace minerals solution (ATCC) ...
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bioRxiv - Immunology 2024Quote: ... Samples were stained with mAbs (30 min at 4°C in the dark) and erythrocytes were lysed (when applicable) using a FACS lysing solution (BD). Isotype-matched nonreactive fluorochrome-conjugated mAbs were used as controls ...
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bioRxiv - Synthetic Biology 2024Quote: ... The expansion conditions of GC organoids were conducted as follows: organoids were suspended with cell recovery solution (354253, BD Falcon), incubated for 30 min on ice in a constant shaking incubator (100 rpm) ...
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bioRxiv - Immunology 2024Quote: ... Blood isolates were then pelleted (500xg for 5 min) and subjected to two rounds of red blood cell lysis (1X Pharm-Lyse solution; BD) prior to antibody staining for flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... followed by 30 min in blocking solution (PBS supplemented with FBS 2%, saponin 0.2%, NaAz 0.1%, 1:1000 FC blocker (BD Bioscience; 553142)) ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were then incubated overnight at 4°C in 100-200 μL of blocking solution containing Rat anti-CD8α-AF647 (1:50, clone 53-6.7, 557682, BD Bioscience) and Rat anti-CD4-PE (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were fixed and permeabilized using BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (554714, BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight in blocking solution with the following primary antibodies (1:100): rat anti-mouse SiglecF (E50-2440, 552126 BD Biosciences), Armenian hamster anti-mouse CD80 (16-10A1 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and incubated overnight at 4°C with the fluorochrome-conjugated antibodies and unconjugated rat and mouse IgG in Perm/Wash solution (BD Biosciences), which was followed by additional 5 min incubation in Perm/Wash solution without antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μl of platelet suspension was mixed with 40 μl of staining solution (HEPES-Tyrode buffer supplemented with 2.5 mM CaCl2, 1:50 PE rat anti-mouse CD41 antibody (BD Biosciences 561850), and 3:50 FITC Annexin V (BD Biosciences 556419)) ...
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bioRxiv - Bioengineering 2021Quote: ... whole blood was drawn from healthy adult volunteers into acid citrate dextrose anticoagulant tubes (ACD, Solution A; BD Franklin Lakes, NJ). Consent from the volunteers was obtained prior to the blood collection ...
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bioRxiv - Immunology 2020Quote: ... and cells were fixed and permeabilized at room temperature for 15 min with Fixation/Permeabilization Solution Kit (BD Biosciences; cat. #554714). For each sample a minimum of 1.2x105 stopping gate events (live CD3+ T-cells ...
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bioRxiv - Plant Biology 2021Quote: ... The protoplasts were resuspended in the same solution without enzyme and sorted with the cytometry (ARIA III Cell sorter, BD bioscience) with the GFP signal and single-cell pattern to isolate the high purity (~ 100% ...