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Citations for Becton, Dickinson and Company :
851 - 900 of 908 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 5X106 cells per mL were washed with cold PBS and blocked in PBS with Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Biosciences) for 15 min at 4°C in the dark ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with FACS staining buffer (PBS supplemented with 2% fetal bovine serum and 1 mM EDTA) and incubated with Fc receptor blocker purified rat anti-mouse anti-CD16/CD32 (BD Biosciences, 553142) for 20 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The plasmid was transfected together with Baculo-Gold bright linearized baculovirus DNA (BD Biosciences) into sf9 insect cells to generate recombinant baculovirus ...
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bioRxiv - Neuroscience 2019Quote: ... injection with α-Ly6G or isotype control antibodies (IP 4 mg/kg; α-Ly6G, clone 1A8, No. 561005; Iso-Ctr, Rat IgG2a, κ, No. 553929; BD Biosciences). Animals were 9-10 months of age at the start of the experiment.
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bioRxiv - Cell Biology 2021Quote: BMSCs were harvested from both femurs of the rats and sorted using a FACS Aria II flow cytometer (BD Biosciences, New Jersey, USA). Bone marrow cells were flushed from the bone marrow cavity after which a red blood cell lysis buffer was used to eliminate red blood cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was prevented using the ells were stained using the BD Pharmingen Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Clone 2.4G2 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were transferred to a 96 well U-bottom plate and Fc receptors on cells were blocked with Rat anti-mouse Fc-Block CD16/CD32-Clone 2.4G2 (BD Pharmigen, San Jose, CA) on ice for 15 minutes ...
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bioRxiv - Immunology 2019Quote: ... Cytokine concentration was quantified in the supernatants by cytometric bead array (CBA) according to the manufacturer’s instructions from the Mouse/Rat Soluble Protein Flex Set System kit (BD Biosciences Pharmingen, Oxford, UK). CBA data was analysed on a MACSQuant analyser with MACSQuantify™ Software and the CBA analysis software package (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... cells were stained with PerCP/Cy5.5 or BV421-conjugated anti–mouse CD4 rat monoclonal antibody (RM4-5; BD Biosciences, Franklin Lakes, NJ, USA). The cells were then fixed and permeabilized with Cytofix/Cytoperm reagent (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in x μL of unlabeled CD16/32 mAb Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™), BD Pharmingen 553141) ...
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bioRxiv - Cell Biology 2020Quote: ... and rat α7 integrin-APC (1/1000; AbLab, Vancouver, Canada) and sorted using a FACS Aria II (BD Biosciences, San Jose, CA, USA). Satellite cells were isolated as CD31− ...
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bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
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bioRxiv - Genomics 2022Quote: ... Each surface staining and intracellular staining step was accompanied by a rat FcBlocking step via an anti-CD32(BD Bioscience Clone: D34-485). Events were acquired on a 5-laser custom BD Fortessa X20 analyzer ...
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bioRxiv - Cancer Biology 2023Quote: Epithelial cell suspensions were stained for viability using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (Thermo #L23105) and BUV737 Rat Anti-Mouse CD45 Clone 30-F11 (BD Biosciences # 568344) and analyzed using a 5-laser Cytek Aurora.
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bioRxiv - Immunology 2023Quote: ... cells were washed in perm buffer (PBS, 0.5% BSA, 0.1% Saponin) and stained for IFN-γ (APC rat anti- mouse IFN-γ; 1:100; BD Biosciences, Cat. 554413) and TNF (PE rat anti-mouse TNF alpha ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
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bioRxiv - Microbiology 2023Quote: ... The homogenized cell suspension was washed and resuspended in FACS buffer containing the PE-conjugated Rat anti-mouse F4/80 antibody (BD Horizon, Cat-565411). Following staining protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... and the cells were stained with antibodies specific for the following proteins: CD4 (PE Rat Anti-Mouse CD4; Clone RM4-5; BD Biosciences; Cat # 553049), H-2Dd (Anti-mouse H-2Dd antibody ...
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bioRxiv - Cell Biology 2021Quote: ... The XPack-GFP plasmid was generated by inserting GFP from pEGFP-N1 (BD Biosciences Clontech) into the XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector (System Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... were plated in buffer (PBS with 0.5% bovine serum albumin and 1 mM EDTA) and non-specific Fc binding was blocked via incubation with mouse anti-rat CD32 (BD Biosciences #550271; 1:200) for 30 minutes on ice ...
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bioRxiv - Microbiology 2021Quote: ... and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution, BD Pharmingen, cat No. 559864). Cell nucleus was stained with DAPI ...
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bioRxiv - Cancer Biology 2019Quote: Glioma TMA sections (4 μm) were stained using a rat monoclonal primary antibody to the integrin β4 subunit (CD104; clone 439-9B; BD Pharmingen, San Jose, CA) at a concentration of 5μg/ml according to a previously described protocol (11) ...
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bioRxiv - Immunology 2020Quote: ... LN staining: macrophages were detected using a primary antibody against CD11b (1/1000, rat anti mouse CD11b conjugated with PE; BD Biosciences, San Jose, California), B-cell follicles were identified using rat anti mouse B220 Alexa F647 (1/1000 ...
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bioRxiv - Bioengineering 2022Quote: ... Macrophages were characterized for presence and absence of canonical markers using flow cytometry PE-Cy5 mouse anti-rat CD45 (BD Biosciences, Mississauga, ON, Canada), BV786 mouse anti-rat CD11b/c (BD Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... Membranes were washed 5x times with TBS-T and stained overnight at 4°C with gentle agitation with primary antibodies: mouse anti-rat CD63 (1:200 dilution, BD Biosciences, Mississauga, ON, Canada), rabbit anti-rat CD9 (1:500 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed with phosphate buffer three times for 10 minutes each and incubated with primary antibody (rat anti-mouse CD31, BD Biosciences, 550274, 1:100) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... upper membrane parts were first probed with rat monoclonal anti-pSMAD1/9 antibody (pS463/pS465, pS465/pS467, 1:1,000, #562508, BD Biosciences, East Rutherford, NJ, USA), or rabbit monoclonal anti-pSMAD2 (S465/467 ...
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bioRxiv - Developmental Biology 2021Quote: Two-hybrid plasmids containing the Drosophila Egl (Egl; CG4051) full-length (pEgl-AD and pEgl-BD), BicD full-length (pBicD-AD and pBicD-BD ...
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bioRxiv - Synthetic Biology 2021Quote: ... grown overnight and transformed with a library of pRS424-SynGal4 plasmids (previously described pADH-Gal4(BD)-fs-Gal4(AD ...
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bioRxiv - Developmental Biology 2022Quote: Full-length dunk was cloned into pGBKT-7 plasmid (a vector containing DNA-binding domain (BD) of GAL4 ...
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bioRxiv - Cell Biology 2023Quote: Plasmids expressing TbPEX1 and TbPEX6 fused to GAL4 activation domain (AD) or GAL4 binding domain (BD) were co-transformed into yeast strains PCY2 and PJ694A ...
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bioRxiv - Microbiology 2021Quote: ... sections were incubated for 30 minutes at room temperature with a rat monoclonal antibody anti-Mouse CD8 (BD Pharmingen, BD Bioscience, San Jose, CA, USA) diluted 1:50 in blocking solution ...
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bioRxiv - Cancer Biology 2023Quote: ... 5X106 cells per mL were washed with cold PBS and blocked in PBS with Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Biosciences) for 15 min at 4°C in the dark ...
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bioRxiv - Immunology 2024Quote: ... cell pellets were re-suspended in FACS buffer and pre-incubated with rat anti-mouse CD16/CD32 Fc Block™ (Cat# 553142, clone 2.4G2; BD Bioscience, Franklin Lakes, NJ, USA) for 10 min on ice and subsequently incubated with specific fluorochrome-conjugated monoclonal anti-mouse antibodies shown in Supplementary Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli was used for genetic manipulation of plasmids and grown in lysogeny broth (LB, BD Difco). When necessary ...
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bioRxiv - Microbiology 2021Quote: ... Signals for 5 fluorescent markers were created by staining with antibodies against the common leukocyte antigen (Rat-anti-mouse CD45, Cat# 550539, clone: 30-F11, BD Pharmingen, San Jose, CA 95131, USA) indirectly labeled with a goat-anti-rat secondary antibody labeled with Alexa Fluor 488 ...
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bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by staining for 30 min at 4°C with the appropriate antibodies: PE-Cy7 rat anti-mouse CD45 (cat # 561868, BD Biosciences, clone 30-F11, 2 ng/μl), BV421 rat anti-mouse CD11b (cat # 562605 ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained on ice for 30min in FACS buffer with 1:100 dilutions of biotin-conjugated-HA and purified rat anti-mouse CD16/CD32 (Mouse BD Fc Block™, Clone 2.4G2, BD Biosciences), then stained on ice for 30min in FACS buffer with 1:1000 dilution of allophycocyanin (APC)-conjugated streptavidin ...
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bioRxiv - Neuroscience 2020Quote: [2] GAL4d from pBPGAL4.1Uw (Addgene plasmid #26226)(Primers:ADdomain,forward,5’-caggcggccgccataTGCATGGATCCGCCAACTTC AACCAGAGTGG-3’, reverse, 5’-tatcgatagacgtca CTACTCCTTCTTTGGGTTCGG-3’; BD domain ...
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bioRxiv - Plant Biology 2019Quote: ... This sequence was annealed and ligated into the Sacl and MIul sites of the reporter plasmid pHIS2 (BD vector). Yeast cells were grown on two-deficiency (-Trp-Leu ...
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bioRxiv - Genomics 2023Quote: ... containing SEND plasmids (1.1 μg/ml for each plasmid) using a 3 ml luer-lok type syringe (Becton Dickinson and Company [BD]) fitted with a 27-gauge needle (BD) ...
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bioRxiv - Plant Biology 2023Quote: ... The plasmid transduction into the above-mentioned yeast strains was confirmed by PCR (KK70/CY179 for AGB1.x-BD, KK281/CY180 for AGG1-AD ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were cloned by monitoring the GFP fluorescence from the reporter plasmid pCAG-EGxxFP with the IRSp53 genome fragment using a fluorescence activated cell sorter [FACSAria (BD)] (Hanawa-Suetsugu et al. ...
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bioRxiv - Microbiology 2022Quote: ... were transformed with recombinant plasmids per manufacturer instructions and grown at 37°C on selective LB agar plates (BD, 244520) and in LB broth (Fisher ...
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bioRxiv - Microbiology 2019Quote: ... A population of parasites expressing the co-transfected Cas9-GFP-containing plasmid were sorted by flow cytometry using a BD Influx cell sorter (BD Biosciences). ~70% of these parasites expressed the HA peptide by IFA.
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bioRxiv - Cell Biology 2022Quote: ... were transformed with one plasmid derived from pPC86 (GAL4-activation domain, AD (‘Prey’)) and pPC97 (GAL4-DNA-binding domain, BD (‘Bait’)) (Chevray and Nathans ...
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bioRxiv - Immunology 2022Quote: Shigella flexneri 2a WT strain 2457T and avirulent plasmid-curated strain BS103 were grown from frozen stocks (−80°C) on Tryptic Soy Agar (TSA) (Difco BD, USA) supplemented with 0.01% Congo Red dye (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293 cells transfected with ABE plasmids were collected from 6-well plate and analyzed on Becton Dickinson LSR II (BD Biosciences) to determine GFP-positive cells ...