Labshake search
Citations for Becton, Dickinson and Company :
851 - 900 of 1192 citations for PLC gamma 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2022Quote: ... The constructs were then co-transformed into yeast AH109 strain and plated on SD selective media and no detectable self-activation was observed (SD -His-Leu, + 2 mM 3-AT or SD -His-Trp, + 40 mM 3-AT (for OsSHR1-BD) or 20 mM 3-AT (for OsSHR2-BD)) ...
-
bioRxiv - Systems Biology 2023Quote: ... 4×106 cells per sample were incubated in blocking solution containing 5% fetal bovine serum and 2% FcBlock (BD Biosciences) in PBS ...
-
bioRxiv - Immunology 2023Quote: ... LLC or CULA cells (2 × 105) resuspended into 50 ul DMEM and enriched with 20% extracellular matrix (Matrigel, BD Biosciences), were intrapleurally injected to the left lung lobe of mice using a 29G needle (BD Biosciences) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were sorted in PBS + 2 % FBS using a BD FACSAriaTM II or BD FACSArisa Fusion (both from BD Biosciences) cell sorters ...
-
bioRxiv - Cancer Biology 2023Quote: ... Polyclonal goat anti-mouse Ig conjugated to APC was used as a secondary antibody (2 μg/mL, BD Pharmigen #550826). For all antibodies ...
-
bioRxiv - Microbiology 2023Quote: ... FITC-A between 2×104–106) or an LSR-Fortessa flow cytometer (BD Biosciences; for Fig 5A and Fig S5C) and data was plotted in FlowJo (ver ...
-
bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H ...
-
bioRxiv - Cancer Biology 2023Quote: Spleens and livers were harvested into ice cold FACS buffer (PBS supplemented with 2% FCS v/v) and then emulsified through a 70μM filter (BD Biosciences), centrifuged and washed in FACS buffer ...
-
bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 2 µg/mL of anti-mouse IgE (553413, clone R35-72, BD Pharmingen) in 0.1 M sodium carbonate buffer pH 9.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were lifted by pipetting in FACS buffer (D-PBS, 2% FBS, 0.1% sodium azide) and analyzed on a FACSCelesta (BD Biosciences). 20,000 events were collected per sample on the cytometer ...
-
bioRxiv - Immunology 2023Quote: ... Staining was quenched by washing in FACS buffer (PBS with 2 mM EDTA and 2% FCS) after which cells were incubated with anti-CD16/32 (Fc block, clone 2.4G2, BD Biosciences) for 15 minutes at 4°C to prevent non-specific binding ...
-
bioRxiv - Developmental Biology 2023Quote: ... one million cells were transferred in 100 μl of FACS buffer supplemented with 0.5μM Y27632 and then added 2 μl Fc Block (BD Pharmingen, 553141) per sample followed by 10 min incubation on ice ...
-
bioRxiv - Microbiology 2023Quote: ... 25 μL of control serum and 25 µL of serum pooled from all mice within a treatment group were pipetted onto a 2-mm diameter plain paper disc (BD) and allowed to dry for 30 minutes ...
-
bioRxiv - Biochemistry 2023Quote: ... For microscopy experiments SDC-lysine media was used (2% glucose, 6.75 g/l yeast nitrogen base without amino acids (291929, BD Difco), 1.92 g/l yeast synthetic drop-out media supplements without lysine (Y1896 ...
-
bioRxiv - Immunology 2023Quote: Bone marrow was isolated from dissected femurs that were gently crushed in PBS with 2% (v/v) FBS and subsequently filtered over a 70μm cell strainer (BD Falcon). Red cell lysis was done by incubating samples with ACK lysis buffer (Quality Biological ...
-
bioRxiv - Immunology 2023Quote: ... The protein binding capacity of the well surface was saturated with 300 μL blocking solution (PBS supplemented with 2% skim milk powder (BD) and 0.1% Tween 20 ...
-
bioRxiv - Immunology 2023Quote: ... and 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) and analyzed on a BD FACS Aria III (BD Biosciences).
-
bioRxiv - Cancer Biology 2023Quote: ... A total of 2 × 106 vector control or shACSL4 cells were mixed 1:1 by volume with Matrigel (BD Biosciences). Each mouse was injected orthotopically into both the flanks ...
-
bioRxiv - Immunology 2023Quote: ... stained cells were resuspended in PBS 2% FBS and DC1 were purified in a FACS Aria II Cell Sorter (BD).
-
bioRxiv - Cell Biology 2023Quote: Cytokine released by the SARS-CoV-2-infected or ApoBD-treated MDMs were measured using human cytokine bead array as per manufacturer’s instruction (BD Biosciences). Briefly ...
-
bioRxiv - Genetics 2023Quote: ... 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution: FITC-conjugated lineage cocktail with antibodies against CD4 (clone H129.19, BD Pharmingen), CD3e (clone 145-2C11 ...
-
bioRxiv - Immunology 2023Quote: ... Cells were stained with pre-optimized concentrations of the following antibodies: PE anti-human IgD (clone IA6-2, BD Biosciences), PE-TXRD anti-human CD10 (clone HI10A ...
-
bioRxiv - Cancer Biology 2023Quote: ... Leukemic cell infiltration was evaluated using immuno-labeling with anti-huCD45 and anti-huCD7 antibodies (see Supplementary Table 2) and a FACS-Canto II flow cytometer (Becton Dickinson, BD). Cells from Femur ...
-
bioRxiv - Microbiology 2023Quote: ... yeast isolates were grown overnight for 16 h from a single colony in 2 mL PD broth (BD, Sparks MA) with shaking at 225 rpm ...
-
bioRxiv - Immunology 2024Quote: ... Cytokine staining was performed with 2 x 106 cells after 3.5h of in vitro stimulation in Opti-MEM media supplemented with Golgiplug (BD Biosciences), 10 ng/ml phorbol 12-myristate 13-acetate (PMA ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: The titles and abstracts of 2 341 articles retrieved from the preliminary search were screened and cross-checked by two independent reviewers (ZL and BD) and discrepancies were resolved through discussion with a third researcher (DM) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2 × 106 cells were harvested and resuspended in 100 μl of ice-cold 20% matrigel (BD Bioscience, Franklin Lakes, NJ) in PBS (Gibco) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Immune cells were resuspended in surface staining mix containing FACS buffer with 1/2 of final volume of Brilliant Stain Buffer (BD), 50 μg/ml anti-mouse CD16/CD32 (2.4G2 ...
-
bioRxiv - Pathology 2024Quote: ... Lentivirally transduced LX-2 cells were sub-cultured until confluent before being flow-sorted on the BD FACSAriaTM (BD Biosciences) to obtain cells with top 10 % highest eGFP expression ...
-
bioRxiv - Cell Biology 2024Quote: Twenty µl of platelet post activation supernatants were incubated with 2.5 µl of phycoerythrin (PE)-conjugated mouse anti-human CD62P (clone AK-2, BD Biosciences), 2.5 µl of PE-conjugated CD61 (clone VI-PL2 ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were blocked for 30 minutes in FACS buffer (2% FBS 1X PBS) containing Fc Block anti-CD16/32 (2.4G2, BD Biosciences) (1:100 dilution) ...
-
bioRxiv - Immunology 2024Quote: ... and then stained for 30 min at 4°C in PBA buffer (PBS, 0.5% BSA, 2 mM EDTA) in the presence of Fc Block (1:50 dilution, BD-Biosciences), using the following antibody combination ...
-
bioRxiv - Cancer Biology 2024Quote: ... with 4′,6-diamidino-2-phenylindole (DAPI) as a viability dye and the following antibodies: anti-CD31 PE (BD Biosciences), anti-CD45 APC (Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were resuspended in 500 m 2% FBS in HBSS and incubated on ice in the dark with 1 l Fc block (BD Biosciences) for 3 minutes ...
-
bioRxiv - Biophysics 2020Quote: ... After incubation cell free supernatant was collected and subjected to sandwich ELISA using IL-2 specific antibody (BD biosciences, CA, USA) as per manufacturer instruction.
-
bioRxiv - Cancer Biology 2021Quote: ... The spheroids were then embedded in a collagen l/Matrigel gel mix at a concentration of approximately 4 mg/ml collagen I and 2 mg/ml Matrigel (BD Bioscience) in 24-well glass-bottomed cell culture plates (MatTek ...
-
bioRxiv - Cancer Biology 2021Quote: ... single cell suspensions (1.5 x 104 cells per ml) were plated in the appropriate medium supplemented with 2% Growth Factor Reduced Matrigel (GFRM; BD Biosciences). 100μl of this mix were added per well in a 96 well ImageLock plate (Essen Biosciences ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were co-stained with 4′,6-diamidino-2-phenylindole (DAPI) to measure DNA content and analyzed with a BD LSRFortessa flow cytometer (BD Biosciences) and FlowJo software (TreeStar).
-
bioRxiv - Systems Biology 2020Quote: ... White cells were resuspended in PBS supplemented with 1% human serum and 2 mM EDTA and analyzed on an LSR Fortessa instrument (BD Biosciences).
-
bioRxiv - Microbiology 2020Quote: ... cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Pacific Blue; BD Biosciences), anti-CD4 (L200 ...
-
bioRxiv - Bioengineering 2021Quote: ... and cells collected from the wells were washed and stained with propidium iodide (2 µg/ml) to distinguish live from dead and run on a flow cytometer (BD FACSCelesta).
-
bioRxiv - Immunology 2021Quote: ... were cultured with 5E5 of YAC-1 target cells (2:1 ratio) in the presence of anti-mouse CD107a-BV421 antibody (564347, BD Biosciences) (1:100 ...
-
bioRxiv - Immunology 2021Quote: ... 1×105 Tconv cells were co-cultured with Treg cells at different ratios in the presence of 2×105 irradiated APCs and 1 µg/ml soluble anti-CD3 antibody (554829, BD Biosciences). The cells were cultured in T-cell complete media which consists of RPMI 1640 medium supplemented with 50 U/ml penicillin ...
-
bioRxiv - Cell Biology 2020Quote: ... Clonal HEK293 cells stably expressing pCRE-DD-zsGreen1 and dCas9-BFP-KRAB were generated by lentiviral transduction and fluorescence activated cell sorting for GFP and BFP on a BD FACS Aria 2 (BD Biosciences). Individual clones were expanded and tested for 1 ...
-
bioRxiv - Immunology 2021Quote: ... For intracellular staining cells were fixed and permeabilized with 2% paraformaldehyde and 70% methanol or with BD Cytofix/ Cytoperm buffer (BD Biosciences) and permeabilization buffer (eBioscience ...
-
bioRxiv - Cell Biology 2020Quote: Protein synthesis of Syndecan-1 was shown by immunocytochemistry using a polyclonal antibody against murine Syndecan-1 (CD138) clone 281-2 (1:50, rat anti-mouse, BD Pharmingen) in co-cultured cells after fixation with ice-cold 99% methanol and heparitinase III (2.4mIU/ml ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cell were resuspended in 200 μl of 2% KSR in PBS and analysed on a 5 laser LSR Fortessa analyser (BD Biosciences). Single cells were gated based on forward and side scatters ...
-
bioRxiv - Developmental Biology 2020Quote: ... 2 partly overlapping holes were punched out by using a 16-gauge Jamshidi bone marrow biopsy needle (BD Biosciences., NJ, USA). The edges of the holes were trimmed with a surgical scalpel and the bottom of the defect was curetted to expose the bleeding subchondral bone ...
-
bioRxiv - Immunology 2022Quote: ... 1–3 ×106 PBMC were stained with a saturating concentration of the following antibodies: CD3 AF700 (clone SP34-2, BD Biosciences), CD4 eFluor450 (clone OKT4 ...