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Citations for Becton, Dickinson and Company :
751 - 800 of 1192 citations for PLC gamma 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... BM cell suspensions (2×107/mL concentration) were treated with FcɣR-blocking agent (anti-CD16/32, clone 2.4G2, BD Biosciences) for 15 min ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were stained in 100ul of staining buffer (PBS + 2 % FBS) containing PE Mouse SNAI2/Slug (BD Biosciences 564615), EZH2 eFluor 660 (Thermo Fisher 50-9867-82) ...
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bioRxiv - Microbiology 2023Quote: ... in 2% FBS in PBS and fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit (BD Biosciences). Then ...
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bioRxiv - Immunology 2023Quote: ... β2 integrin blockade was performed with Purified NA/LE Rat Anti-Mouse CD18 (clone GAME-46, BD Pharmingen, 555280) and the isotype control antibody Purified NA/LE Rat Ig1 ...
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bioRxiv - Microbiology 2022Quote: ... (yeast nitrogen base without amino acids containing 0.17% ammonium sulfate [BD Difco], 2% glucose, 0.13% casamino acids [BD Difco]) was used for yeast culture for mannoprotein preparation.
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bioRxiv - Immunology 2023Quote: ... All experimental steps were performed in a 50/50 mixture of PBS + 2% FBS and Brilliant Buffer (BD Bioscience). Antigen probes were prepared individually and mixed together after multimerization with 5μM free D-biotin (Avidity LLC ...
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bioRxiv - Cancer Biology 2023Quote: ... 1-2×106 cells from tumors were incubated with Fixable Viability Stain UV440 (BD Bioscience, San Jose, CA, USA) for 15 minutes at room temperature in a 96-well plate ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed in PBS+2% FCS and permeabilised for 10 minutes in 70ul of Cytofix/Cytoperm (BD Biosciences). After washing in 2mL of PermWash ...
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bioRxiv - Neuroscience 2023Quote: Trial types differing by the magnitude of the difference in rank between items (i.e. distance 1: AB, BA, BC, CB, CD, DC, DE, ED, EF, FE, FG, GF; 2: AC, CA, BD, DB ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cryopreserved human hepatocytes (Lot BD195: 2-year-old, Hispanic female, 1 × 106 hepatocytes, BD Biosciences, Ann Arbor, MI, USA) were transplanted under anesthesia into 2-to 4-week-old hemizygous cDNA-uPA/SCID mice through the spleen ...
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bioRxiv - Immunology 2023Quote: ... 5 x104 – 2 x105 leukocytes were examined per sample with a FACSCanto II (BD Biosciences, Franklin Lakes, NJ, USA), according to established multi-color protocols (Cossarizza et al. ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in sort buffer (2% FBS in PBS + 2mM EDTA) and analysed on the Fortessa X20 (BD) flow cytometer ...
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in surface staining mix containing FACS buffer with 1/2 of final volume of Brilliant Stain Buffer (BD), 50 μg/ml anti-mouse CD16/CD32 (2.4G2 ...
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bioRxiv - Immunology 2023Quote: ... 2) As the MojoSort antibodies are biotinylated we replaced the Qa2-bio antibody with Qa2-BV421 (BD, Cat. 743309). Using qPCR and FACS we ensured that the positively selected (lineage positive ...
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bioRxiv - Immunology 2023Quote: ... 2×106 cells/well (6-well plate) were cultured with 1μg/mL of anti-CD3 mAb (553057, BD Biosciences) plus 10 ng/mL of mouse IL-2 (Z02764 ...
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bioRxiv - Neuroscience 2023Quote: ... Digested suspensions were subsequently triturated by sterilized syringes with 20G 1/2 needle (BD Biosciences, Franklin Lakes, NJ, USA) and washed with DMEM to harvest mononuclear cells ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 enriched or unenriched cells were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). From infant thymus and PBMC donors up to 5 populations were sorted after doublet ...
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bioRxiv - Immunology 2024Quote: ... cells were blocked with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences). For detection of endogenous p65 ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences) and stained with cell surface marker detecting fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2024Quote: ... and blocking with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences). Intracellular proteins were stained with fluorophore-conjugated antibodies overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... anti-MASP-2 mAb F3-5 (Peter et al. 2022) and an irrelevant mouse IgG1 mAb (BD Biosciences, 557273) were coupled to to Dynabeads M-280 streptavidin (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... Twenty 8-11 months-old Gata1low mice were randomly divided in two groups which were treated either with vehicle (2% v/v dimethyl sulfoxide in H2O) or with biotinylated mAb RB40.34 (BD Pharmigen ...
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bioRxiv - Plant Biology 2020Quote: 10-fold serial dilutions (OD600=1, 10-1, 10-2, 10-3) of yeast sample harbouring a DNA-binding domain (BD)-AtCIPK16 and either an activation domain (AD)-AtCBLs or an empty vector (AD ...
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bioRxiv - Bioengineering 2022Quote: ... Brooks Lifesciences) were coated with 200 μL of 2 mg/mL type 1 rat tail collagen (354249, Corning/BD Biosciences) diluted in DMEM equilibrated in 1/10 v/v of 7.5% w/v sodium bicarbonate solution (#25080-060 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were fixed in 2% PFA and subsequently acquired using a BD LSRFortessa flow cytometer (BD Biosciences, San Jose, CA). Flow data were analyzed using FlowJo v10 software (Tree Star ...
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bioRxiv - Immunology 2021Quote: ... cells were either resuspended in 400μl of PBS 2%FCS 2mM EDTA and analyzed on Fortessa-X20/Symphony cytometers (BD Biosciences) or in RPMI media and used for cell sorting on a FACSAria II (BD ...
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bioRxiv - Immunology 2021Quote: ... Vα7.2+ cells were pre-incubated for 1 h and 30 min with LukED at 0.312 μg/mL before adding to the bacteria-pulsed THP-1 and co-culture at 2:1 ratio for 24 h in the presence of 1.25 μg/mL anti-CD28 mAb (L293, BD Biosciences). Monensin and brefeldin A (both from BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... whole blood was stained within 2 hours of blood draw for the surface markers CD45 (PerCP; DO58-1283; BD Biosciences), CD3 (FITC ...
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bioRxiv - Immunology 2020Quote: ... In experiments testing double recombinants SARS-CoV2 vectors IL-2 antibody was not included and PE-CF594 anti-mouse IL-4 (clone 11B11, 562450, BD) and BV421 rat anti mouse IL-10 (clone JES5-16E3 ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed in 2% paraformaldehyde and were examined in a Canto II flow cytometer (BD Bioscience, San Jose, CA), following a previously reported protocol [49] ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mL of cells at 100,000 cells/mL in PBS-BSA was loaded in a 3 mL syringe (BD, #309657). A 125 μm Drop-seq microfluidic device was used for droplet generation (31) ...
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bioRxiv - Immunology 2021Quote: ... at 100ng/mL and IL-2 (100IU/mL) or expanded by plate-bound anti-CD3 mAb at 2μg/mL (BD Pharmingen) and soluble anti-CD28 mAb at 1μg/mL (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... and microglia (CD45med CD11b+were sorted into collection tubes coated with FCS and filled with complete RPMI 1640 medium (1% penicillin, streptomycin, 0.1% 2-ME) with 20% FCS using a FACSAria device (BD Biosciences). Purity of sorted populations was routinely above 95% ...
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bioRxiv - Immunology 2020Quote: ... for 2 h and 4 h respectively at 37°C in 5% CO2 and exposed to brefeldin A (BD Biosciences) as described in previous studies ...
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bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured in T cell media supplemented with soluble anti-mouse CD28 (2 μg/ml, Clone: 37.51, BD Pharmingen) and 30 U/ml rhIL-2 (Roche ...
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bioRxiv - Bioengineering 2021Quote: ... at 2×106 cells/ml in wells coated with anti-mouse CD3e (1 μg/ml, Clone: 145-2C11, BD Pharmingen).
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bioRxiv - Bioengineering 2020Quote: ... The concentrations of cytokines IL-2 and IFN-γ were quantified using the corresponding ELISA kits (BD, 555190 and 555142).
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibodies diluted in PBS containing 2% NHS overnight (1:500 rabbit anti-Iba1, Abcam, ab178846; 1:200 mouse anti-Map2, BD Pharmagen ...
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bioRxiv - Immunology 2020Quote: ... 2 x 106 cells were cultured in 200μL final volume in 5mL polypropylene tubes (BD Biosciences, San Diego, CA, U.S.A) in the presence of anti-CD28 (1μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... xenograft models were generated by injecting SKOV3 cells [2 × 106 cells mixed 1:1 (v/v) with Matrigel (BD Biosciences)] subcutaneously ...
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bioRxiv - Systems Biology 2021Quote: ... The concentration of the cell suspension was adjusted to 2×106 cells ml-1 and subsequently aspirated into a 3 ml luer-lock syringe (BD).
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bioRxiv - Genomics 2021Quote: ... stimulated cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Alexa700; BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: 100 μl of washed and number-adjusted yeast suspension were injected into each caterpillar’s distal right proleg with a 30G1/2” needle (BD Microlance) and a 1 ml NORM-JECT syringe ...
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bioRxiv - Genomics 2020Quote: Primary human bronchial epithelial basal cells (Passage 1 or 2) were expanded on flasks coated with collagen I (BD Biocoat) in bronchial epithelial growth medium (BEGM) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were incubated on a cold block for 2-5 minutes before being plated in a 96 (12 x 8) grid on Omnitrays containing LB (BD, Franklin Lakes ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then washed with FC buffer (PBS supplemented with 2% HI-FBS and 0.05% NaN3) and resuspended in Fixation Buffer (BD Pharmingen)for 5 min at 4C° in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... and Western blots (WBs): monoclonal Abs (mAbs) anti-flotillin-2 (610383; WB: 1/1000) and anti-GM130 (610823; IF: 1/500) from BD Biosciences ...
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bioRxiv - Developmental Biology 2022Quote: ... E.coli strain Rosetta™ 2(DE3)pLysS bearing pET21d-FGF10 was grown in 2x YT medium (BD Biosciences, Cat#244020) with Carbenicillin (50µg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... Collagenase activity was quenched by dilution with FACS buffer (PBS, 0.5% FBS, 2 mM EDTA) and digestion products were filtered through 70 µm nylon filters (BD Biosciences). Cells were again washed with FACS buffer and immediately processed for immunostaining first by blocking Fc receptor binding with rat anti-mouse CD16/CD32 (eBioscience ...