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Citations for Becton, Dickinson and Company :
8551 - 8600 of 9716 citations for Anti PIF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... the cell culture mixture was stained with an antibody recognizing the neural cell adhesion molecule (NCAM/CD56; BD Pharmingen; Table S1), and the myogenic progenitor (CD56+ ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4℃ after 1h blocking in 5% skimmed milk (Becton, Dickinson and Company, USA) at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were fixed in 4% paraformaldehyde for 30 minutes and stained with PE-conjugated or purified CD45 antibody (eBioscience, 30-F11 or BD Biosciences) to ascertain the generation of haematopoietic progeny ...
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bioRxiv - Cell Biology 2019Quote: ... cells were stained with a 1:50 dilution of phycoerythrin (PE)-conjugated mouse monoclonal antibody against human TRAIL (550516, BD Biosciences). For immunophenotyping of MSCs the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770 ...
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bioRxiv - Genomics 2021Quote: ... tissues were blocked (3% bovine serum albumin in 5% normal goat sera and 0.1% Triton) and incubated overnight at 4°C with primary antibodies: CD31 (Neomarkers, RB-10333-P0) or CDH1 (BD Biosciences, 6315829). Slides were washed and incubated with Cy3-goat-anti-mouse or anti-rabbit-conjugated secondary antibodies (Jackson Immunoresearch Laboratories ...
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bioRxiv - Immunology 2021Quote: Post-culture cells were pelleted in V-bottom 96-well plates and resuspended in 30 μl of antibodies at pre-optimized concentrations and diluted in Brilliant Stain Buffer (BD Biosciences), then incubated for 15’ at RT ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with a panel of antibodies (Supplementary Table 1) in flow cytometry staining buffer with BD Horizon Brilliant Stain Buffer Plus (BD Biosciences) for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... one section out of ten was incubated with BrdU antibodies (BrdU, 1/1,000, CldU, 1/500, Accurate Chemical; IdU, 1/500, BD Bioscience). Bound antibodies were visualized with Cy3-goat anti-rat antibodies (1/1,000 ...
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bioRxiv - Microbiology 2020Quote: ... for 5 min and incubated for 30 min with optimal concentrations of fluorochrome-conjugated antibodies (Table S2) diluted in Brilliant Stain Buffer (BD Biosciences). After three washes with FACS buffer ...
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then washed twice 5 min in Tris buffer saline containing 0.01% Triton X-100 (TBST) and incubated with the primary antibody over night at 4°C (human and mouse a-Syn: 610786 BD Biosciences). After incubation with the first antibody ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with fluorochrome-conjugated (BV421, BV711, FITC, PE, PE-Cy7, allophycocyanin [APC], APC-Cy7) antibodies against mouse CD45 (30-F11; BD Biosciences), Ly6C (AL-21 ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were then incubated with primary antibodies (ACE2, ab15438, Abcam, UK; HCG beta, ab9582, Abcam, UK; HLA-G, 557577, BD Pharmingen) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells or MNC from CML patients or HD were incubated with the following antibodies: 5 µL CD45-PerCP (2D1, BD Biosciences), 2.5 µL CD34-FITC (AC136 ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interest were visualized after incubation with primary antibodies (α-synuclein 1:1000 BD Biosciences (San Jose, CA, USA) #610787 ...
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bioRxiv - Bioengineering 2021Quote: ... at 1:1 E:T ratio at 37°C with 5% CO2 in the presence of a CD107a antibody for 1 hr before treatment with brefeldin A (10 µg/ml; BD Biosciences) and monensin (0.7µg/ml ...
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bioRxiv - Bioengineering 2021Quote: ... An antibody panel for macrophage and T cell polarization was also stained for consisting of: F4/80 BUV395 (T45-2342, BD Biosciences), CD86 BV786 (GL-1 ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining using antibodies detecting Active Caspase-3 (C92-605) and BCL2L11 (Y36) was performed using BD Cytofix/Cytoperm (BD Biosciences) or eBioscience FoxP3/Transcription Factor Staining Buffer Set (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... and stained for 30 min on ice with a cocktail of antibodies diluted in a 1:1 mixture of FACS buffer and the Brilliant Stain Buffer (BD Biosciences). The antibody cocktail consisted of APC/Cy7-CD3 (SK7 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with antibodies according to the manufacturer’s specifications and analyzed with a BD FACSCelesta™ Flow Cytometer (BD Biosciences). Engraftment and lineage analysis was determined by staining peripheral blood with CD45.1 (BioLegend Cat#110708 ...
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bioRxiv - Cell Biology 2022Quote: Bone marrow cells from mice were labeled with a cocktail of biotin-conjugated lineage marker antibodies in combination with Streptavidin Particles Plus (BD, 557812) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... fixable viability dye was included with the surface staining antibodies (20 minutes, RT) and intracellular staining used the Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... barcoded and permeabilized as previously described.31 The samples were stained with the indicated antibodies and analyzed with a BD LSR Fortessa cytometer (BD Biosciences) equipped with 488 nm ...
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bioRxiv - Immunology 2022Quote: ... The cells were then blocked with 5% BSA for 20 minutes before being stained with antibodies against CD19-PE (BD-555413), CD3-BV510 (BD-564713) ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used herein at 1:200 dilution unless otherwise indicated: Fixed viability stain-700 (FVS700, BD, 1:1000), anti-IL-17A (TC11-18H10 ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with fluorochrome-conjugated antibodies for 30min at 4°C in the dark in brilliant stain buffer (BD Biosciences). Prior to fixation and permeabilization for intracellular staining with Cytofix/Cytoperm (BD Bioscience) ...
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bioRxiv - Immunology 2019Quote: ... Cells were initially stained with fluorochrome-conjugated antibodies against surface receptors (see Table S1) in BD Brilliant stain buffer (BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated in PGB for 25 min with antibodies against Lewis-X (1:20, LeX/CD15 FITC, MMA; BD Bioscience), CD133-APC (1:50,13A4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was blocked with 5% non-fat dry milk (Marvel) and subsequently incubated (O/N, 4°C) with primary antibodies HIF-1α (BD Biosciences) Lamin A (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... these cells were stimulated with a 10 μg/ml solution of CD28 and CD49d co-stimulatory antibodies (both from BD Biosciences) and 1ug/ml of 15-mer overlapping peptide pools spanning either the SARS-CoV-2 spike (S) ...
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bioRxiv - Immunology 2020Quote: ... For flow cytometry analysis first Fc receptors were blocked using α-CD16/CD32 antibody (clone 2.4G2, BD Biosciences, Bedford, MA, USA). The cells were subsequently washed and stained for following surface markers with directly conjugated antibodies against CD19/B220 (PerCP Cy5.5 or eFluor 450) ...
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bioRxiv - Immunology 2020Quote: ... After subsequent washing step cells were labelled with primary and secondary antibodies or streptavidin-fluorochrome conjugates and were used for flow cytometry analysis (LSR-II, BD Biosciences) or sorting (FACSAria ...
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bioRxiv - Cancer Biology 2021Quote: ... Blood samples from the same animals were also stained with the same antibody panel before red blood cell lysis using BD Pharm Lyse lysis buffer (BD Biosciences). All results were acquired using a BD LSRFortessa (BD Biosciences) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated during 30 minutes at 4°C with the following primary antibodies: CD11b (clone M1/70, AF647, #557686, BD Pharmingen), CD45 (clone 30-F11 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated with the following primary antibodies during 30 minutes at 4°C: CD11b (clone M1/70, APC-Cy7, #557657, BD Pharmingen), CD45 (clone 30-F11 ...
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bioRxiv - Neuroscience 2022Quote: ... Syn1 [a mouse monoclonal antibody that recognizes residues 91-99 from the NAC region of human α-synuclein (51)] (BD Biosciences) at 1:4,000 and PER4 at 1:4,000 ...
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bioRxiv - Immunology 2022Quote: ... MLN cells and lung CD11c+ cells were stained for flow cytometry analysis as described above using following fluorophore-conjugated antibodies DAPI (BD Biosciences), CD45 (30-F11 ...
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bioRxiv - Immunology 2022Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1 perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before acquisition using the BD Symphony Flow Cytometer and the associated BD FACS Diva software ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunohistochemistry (IHC) was performed on FFEP blocks using mouse monoclonal antibodies SMARCB1/INI1 (Clone 25/BAF47, dilution 1:200, BD Bioscience) and CD70 (Clone 301731 ...
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bioRxiv - Cell Biology 2022Quote: ... cell sorting was employed by labeling intact cells with the fluorescently conjugated HER2 antibody ErbB2 PE-Vio770 and then subjected to fluorescence-activated cell sorting using a FACS Aria III (BD Biosciences), selecting the cells in the top 10% of HER2 expression to generate MDA-MB-231-HER2 stable cells.
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Cardiac fibroblasts regulate cardiomyocyte hypertrophy through dynamic regulation of type I collagenbioRxiv - Cell Biology 2022Quote: ... and the cells were labeled with antibodies as also described above and were then analyzed using a BD FACSCanto cytometer running BD FACSDiVa software (BD Biosciences), using a violet (405 nm ...
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bioRxiv - Genomics 2022Quote: ... This was followed by the secondary labeling step with 1:200 dilution of Streptavidin PE-Cy7 antibody (BD Biosciences, Cat#: 557598). Cells were isolated and sorted using an Influx Cell Sorter Instrument (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with 500 μL permeabilization buffer (from kit described above) and stained with 150 μL permeabilization buffer containing 1:400 Ki67-Alexa-Fluor antibody (561126, BD Biosciences) for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained with fluorochrome-conjugated antibodies or influenza HA-specific (HA518–526) H-2Kd tetramer-IYSTVASSL (MBL) reagent and analyzed using a flow cytometer (BD Biosciences). BMDCs (105 cells ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 and MIP-2 were measured using paired antibodies and recombinant cytokines according to the manufacturer’s recommendations (BD PharMingen Systems) (11).
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bioRxiv - Cancer Biology 2019Quote: DMS-53 cells transduced with DARPP-32 shRNAs or exogenously overexpressed DARPP-32 isoforms were stained with Annexin V-FITC antibodies (BD Biosciences) to assess apoptosis ...
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bioRxiv - Biochemistry 2019Quote: ... After washing with flow cytometry buffer cells were incubated with secondary antibodies for 30 min on ice and wash twice and analyzed by FACScanto instrument (BD Biosciences). Cell fixation and permeabilization was carried out using the Fixation/Permeabilization Solution Kit (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... HMLER CD44low/high cells stained with CD24-APC and CD44-PE antibodies were sorted by FACS using an Aria IIu (BD Biosciences) to obtain isolated CD44high/CD24low and CD44low/CD44high cell populations ...
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bioRxiv - Immunology 2020Quote: Colonic LP samples (2 million cell/staining) were stained at 4°C for 20 min with the following antibodies: Ly6C-FITC (AL-21, BD Pharmingen), F4/80-PE (BM8 ...