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Citations for Becton, Dickinson and Company :
8501 - 8550 of 9655 citations for Anti PIF1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cells were incubated with fluorochrome-conjugated (BV421, BV711, FITC, PE, PE-Cy7, allophycocyanin [APC], APC-Cy7) antibodies against mouse CD45 (30-F11; BD Biosciences), Ly6C (AL-21 ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were then incubated with primary antibodies (ACE2, ab15438, Abcam, UK; HCG beta, ab9582, Abcam, UK; HLA-G, 557577, BD Pharmingen) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells or MNC from CML patients or HD were incubated with the following antibodies: 5 µL CD45-PerCP (2D1, BD Biosciences), 2.5 µL CD34-FITC (AC136 ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interest were visualized after incubation with primary antibodies (α-synuclein 1:1000 BD Biosciences (San Jose, CA, USA) #610787 ...
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bioRxiv - Bioengineering 2021Quote: ... at 1:1 E:T ratio at 37°C with 5% CO2 in the presence of a CD107a antibody for 1 hr before treatment with brefeldin A (10 µg/ml; BD Biosciences) and monensin (0.7µg/ml ...
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bioRxiv - Bioengineering 2021Quote: ... An antibody panel for macrophage and T cell polarization was also stained for consisting of: F4/80 BUV395 (T45-2342, BD Biosciences), CD86 BV786 (GL-1 ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining using antibodies detecting Active Caspase-3 (C92-605) and BCL2L11 (Y36) was performed using BD Cytofix/Cytoperm (BD Biosciences) or eBioscience FoxP3/Transcription Factor Staining Buffer Set (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... and stained for 30 min on ice with a cocktail of antibodies diluted in a 1:1 mixture of FACS buffer and the Brilliant Stain Buffer (BD Biosciences). The antibody cocktail consisted of APC/Cy7-CD3 (SK7 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with antibodies according to the manufacturer’s specifications and analyzed with a BD FACSCelesta™ Flow Cytometer (BD Biosciences). Engraftment and lineage analysis was determined by staining peripheral blood with CD45.1 (BioLegend Cat#110708 ...
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bioRxiv - Cell Biology 2022Quote: Bone marrow cells from mice were labeled with a cocktail of biotin-conjugated lineage marker antibodies in combination with Streptavidin Particles Plus (BD, 557812) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... fixable viability dye was included with the surface staining antibodies (20 minutes, RT) and intracellular staining used the Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... barcoded and permeabilized as previously described.31 The samples were stained with the indicated antibodies and analyzed with a BD LSR Fortessa cytometer (BD Biosciences) equipped with 488 nm ...
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bioRxiv - Immunology 2022Quote: ... The cells were then blocked with 5% BSA for 20 minutes before being stained with antibodies against CD19-PE (BD-555413), CD3-BV510 (BD-564713) ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used herein at 1:200 dilution unless otherwise indicated: Fixed viability stain-700 (FVS700, BD, 1:1000), anti-IL-17A (TC11-18H10 ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with fluorochrome-conjugated antibodies for 30min at 4°C in the dark in brilliant stain buffer (BD Biosciences). Prior to fixation and permeabilization for intracellular staining with Cytofix/Cytoperm (BD Bioscience) ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated in PGB for 25 min with antibodies against Lewis-X (1:20, LeX/CD15 FITC, MMA; BD Bioscience), CD133-APC (1:50,13A4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was blocked with 5% non-fat dry milk (Marvel) and subsequently incubated (O/N, 4°C) with primary antibodies HIF-1α (BD Biosciences) Lamin A (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... these cells were stimulated with a 10 μg/ml solution of CD28 and CD49d co-stimulatory antibodies (both from BD Biosciences) and 1ug/ml of 15-mer overlapping peptide pools spanning either the SARS-CoV-2 spike (S) ...
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bioRxiv - Immunology 2020Quote: ... For flow cytometry analysis first Fc receptors were blocked using α-CD16/CD32 antibody (clone 2.4G2, BD Biosciences, Bedford, MA, USA). The cells were subsequently washed and stained for following surface markers with directly conjugated antibodies against CD19/B220 (PerCP Cy5.5 or eFluor 450) ...
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bioRxiv - Immunology 2020Quote: ... After subsequent washing step cells were labelled with primary and secondary antibodies or streptavidin-fluorochrome conjugates and were used for flow cytometry analysis (LSR-II, BD Biosciences) or sorting (FACSAria ...
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bioRxiv - Cancer Biology 2021Quote: ... Blood samples from the same animals were also stained with the same antibody panel before red blood cell lysis using BD Pharm Lyse lysis buffer (BD Biosciences). All results were acquired using a BD LSRFortessa (BD Biosciences) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated during 30 minutes at 4°C with the following primary antibodies: CD11b (clone M1/70, AF647, #557686, BD Pharmingen), CD45 (clone 30-F11 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated with the following primary antibodies during 30 minutes at 4°C: CD11b (clone M1/70, APC-Cy7, #557657, BD Pharmingen), CD45 (clone 30-F11 ...
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bioRxiv - Neuroscience 2022Quote: ... Syn1 [a mouse monoclonal antibody that recognizes residues 91-99 from the NAC region of human α-synuclein (51)] (BD Biosciences) at 1:4,000 and PER4 at 1:4,000 ...
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bioRxiv - Immunology 2022Quote: ... MLN cells and lung CD11c+ cells were stained for flow cytometry analysis as described above using following fluorophore-conjugated antibodies DAPI (BD Biosciences), CD45 (30-F11 ...
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bioRxiv - Immunology 2022Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1 perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before acquisition using the BD Symphony Flow Cytometer and the associated BD FACS Diva software ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunohistochemistry (IHC) was performed on FFEP blocks using mouse monoclonal antibodies SMARCB1/INI1 (Clone 25/BAF47, dilution 1:200, BD Bioscience) and CD70 (Clone 301731 ...
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bioRxiv - Cell Biology 2022Quote: ... cell sorting was employed by labeling intact cells with the fluorescently conjugated HER2 antibody ErbB2 PE-Vio770 and then subjected to fluorescence-activated cell sorting using a FACS Aria III (BD Biosciences), selecting the cells in the top 10% of HER2 expression to generate MDA-MB-231-HER2 stable cells.
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Cardiac fibroblasts regulate cardiomyocyte hypertrophy through dynamic regulation of type I collagenbioRxiv - Cell Biology 2022Quote: ... and the cells were labeled with antibodies as also described above and were then analyzed using a BD FACSCanto cytometer running BD FACSDiVa software (BD Biosciences), using a violet (405 nm ...
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bioRxiv - Genomics 2022Quote: ... This was followed by the secondary labeling step with 1:200 dilution of Streptavidin PE-Cy7 antibody (BD Biosciences, Cat#: 557598). Cells were isolated and sorted using an Influx Cell Sorter Instrument (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with 500 μL permeabilization buffer (from kit described above) and stained with 150 μL permeabilization buffer containing 1:400 Ki67-Alexa-Fluor antibody (561126, BD Biosciences) for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained with fluorochrome-conjugated antibodies or influenza HA-specific (HA518–526) H-2Kd tetramer-IYSTVASSL (MBL) reagent and analyzed using a flow cytometer (BD Biosciences). BMDCs (105 cells ...
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bioRxiv - Immunology 2020Quote: Colonic LP samples (2 million cell/staining) were stained at 4°C for 20 min with the following antibodies: Ly6C-FITC (AL-21, BD Pharmingen), F4/80-PE (BM8 ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 4°C and secondary antibodies for 30 min in RT in Perm/Wash buffer (554714, BD Biosciences) containing 5% FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... which were then stained with the above-mentioned antibodies and LT-HSCs were sorted using a BD FACS Aria III (BD Biosciences).
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bioRxiv - Immunology 2020Quote: PBMCs or purified T cells were stained for 30 minutes on ice with the antibodies specific for extracellular proteins in Brilliant Stain Buffer (BD, 563794). Following incubation ...
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bioRxiv - Immunology 2020Quote: We centrifuged the samples and resuspended each in 1 mL of an antibody master mix consisting of Brilliant Buffer (BD Biosciences) and anti-CCR6 - PECy7 (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... the cell surface of freshly-prepared epidermal cells was labeled with the following antihuman protein antibodies (clone, manufacturer): FITC–conjugated CD1a (HI149) and PE–conjugated HLA-DR (G46-6, both BD Pharmingen), PE–CF594–conjugated CD3 (UCHT1 ...
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bioRxiv - Cell Biology 2020Quote: ... The purity of the cells was confirmed by staining cells with PE-CD71 and allophycocyanin (APC)-Ter119 antibodies (all from BD Biosciences) by flow cytometry ...
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bioRxiv - Cell Biology 2020Quote: ... Kidney sections were blocked (10% normal goat serum and 1% bovine serum albumin (BSA) in PBS) and incubated overnight at 4°C with primary antibody ((Ly6G, 1:10; BD/Pharmingen), FITC-C3 (1:200 ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with the medium and stained with subsequent antibodies/reagents using the Brilliant Stain Buffer Plus (BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Cells were then incubated with 5ul human Fc Block with 95ul 2% FBS in PBS at 4°C for 15 mins before staining with SCMK and AbSeq antibodies (BD Biosciences) at 4°C for 60 min ...
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bioRxiv - Immunology 2021Quote: ... The stained cells were subsequently washed with FACS buffer to remove unbound antibodies and probe and then filtered through 70-μm cell mesh (BD Biosciences) before processed in the FACS sorter ...
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bioRxiv - Immunology 2021Quote: ... cryo-preserved PBMCs were first thawed at 37°C in a water bath and washed with an RPMI medium containing 10% fetal bovine sera (FBS) following incubation with fluorescently-labeled antibodies (BD Biosciences) against cell surface markers for CD3 (PE-Cy7) ...
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bioRxiv - Developmental Biology 2021Quote: Mouse femur single cells were obtained as described above in the flow cytometry section and stained with biotin-conjugated antibodies for Lin (Ter119, Gr-1, B220, CD3e) (BD, 559971) followed by negative selection using streptavidin-coated magnet beads (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: ... each sample was stained with the corresponding primary antibody-dye conjugates diluted in 100 μl Pharmingen stain buffer (BD Biosciences, #554657). After 1 h staining at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... and 2 mM KH2PO4)-FBS 5% for 15 min and then stained for surface expression with fluorophore-marked antibodies against SLAMF1 (BD, 559572), CD31 (BD ...
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bioRxiv - Immunology 2020Quote: ... The cells (2-4 × 105 cells per well of a 96-well plate) were added to the ELISpot plate containing an immobilized primary antibodies to either IFN-γ or IL-4 (BD), and were exposed to various stimuli (e.g ...
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bioRxiv - Immunology 2021Quote: ... 4 and 6 hours and cytokines detected by intracellular cytokine staining (see Table S2 for antibodies list) using Fixation/Permeabilization Solution Kit (BD Biosciences), unstimulated cells were used as a control.