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• Sensitization with an allogeneic MHC class I molecule induces anti-tumor immunity in the absence of PD-1 in mice

  Komang Alit Paramitasari, Yasumasa Ishida, bioRxiv - Immunology 2023

  Quote: ... for 10 min and directly stained with APC-conjugated anti-mouse CD45 (clone 30-F11, BD Pharmingen) to specifically stained leukocytes ...
• The adaptive immune response to Trichuris in wild versus laboratory mice: An established model system in context

  Iris Mair, et al., bioRxiv - Immunology 2023

  Quote: ... Cytokines were analysed using the Cytometric Bead Array (CBA) Mouse/Rat soluble protein flex set system (BD Bioscience), which was used according to the manufacturer’s instructions ...
• Altered DNA methylation underlies monocyte dysregulation and innate exhaustion memory in sepsis

  Blake A. Caldwell, Yajun Wu, Jing Wang, Liwu Li, bioRxiv - Immunology 2023

  Quote: ... F4/80 (BV711; BD Biosciences #565612), CD38 (BV750 ...
• Altered DNA methylation underlies monocyte dysregulation and innate exhaustion memory in sepsis

  Blake A. Caldwell, Yajun Wu, Jing Wang, Liwu Li, bioRxiv - Immunology 2023

  Quote: ... Panel 1 samples were measured with a FACS Canto II (BD Biosciences), panel 2 samples were measured with an Aurora 4L 16V-14B-10YG-8R (Cytek) ...
• Altered DNA methylation underlies monocyte dysregulation and innate exhaustion memory in sepsis

  Blake A. Caldwell, Yajun Wu, Jing Wang, Liwu Li, bioRxiv - Immunology 2023

  Quote: All flow cytometry data were analyzed with FlowJo (BD Life Sciences). For ex vivo BMMC experiments ...
• Generation of antigen-specific memory CD4 T cells by heterologous immunization enhances the magnitude of the germinal center response upon influenza infection

  Linda M. Sircy, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were stained on ice for 30min in FACS buffer with 1:100 dilutions of biotin-conjugated-HA and purified rat anti-mouse CD16/CD32 (Mouse BD Fc Block™, Clone 2.4G2, BD Biosciences), then stained on ice for 30min in FACS buffer with 1:1000 dilution of allophycocyanin (APC)-conjugated streptavidin ...
• Generation of antigen-specific memory CD4 T cells by heterologous immunization enhances the magnitude of the germinal center response upon influenza infection

  Linda M. Sircy, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were stained on ice for 30min in FACS buffer with 1:100 dilutions of biotin-conjugated-HA and purified rat anti-mouse CD16/CD32 (Mouse BD Fc Block™, Clone 2.4G2, BD Biosciences), then stained on ice for 30min in FACS buffer with 1:1000 dilution of allophycocyanin (APC)-conjugated streptavidin ...
• Generation of antigen-specific memory CD4 T cells by heterologous immunization enhances the magnitude of the germinal center response upon influenza infection

  Linda M. Sircy, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were analyzed on LSRFortessa™ X-20 and LSRFortessa™ (BD Biosciences) cytometers ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BUV737 Rat Anti-Mouse IgG1 (Clone A85-1; BD Biosciences ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BV421 Rat Anti-Mouse IgG1 (Clone A85-1; BD Biosciences ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BUV395 Mouse Anti-Mouse CD45.2 (Clone 104; BD Biosciences ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BUV395 Rat Anti-Mouse IgD (Clone 11-26c.2a; BD Biosciences ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BV421 Armenian Hamster Anti-Mouse CD95 (Clone Jo2; BD Biosciences ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BV421 Rat Anti-Mouse CD35 (Clone 8C12; BD Biosciences ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BV510 Armenian Hamster Anti-Mouse CD95 (Clone Jo2; BD Biosciences ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... Alexa Fluor® 647 Mouse anti-Bcl-6 (Clone K112-91; BD Biosciences ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BV786 Rat Anti-Mouse CD138 (Syndecan-1) (Clone 281-2; BD Biosciences ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... Biotin Rat Anti-Mouse CD138 (Syndecan-1) (Clone 281-2; BD Biosciences ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... RRID: AB_657740) BV650 Rat Anti-Mouse CD38 (Clone 90; BD Biosciences ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... CD138 (clone 281-2, BD Biosciences), TER-119 (clone TER-119 ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were then incubated with Alexa Fluor 647 anti-BCL6 (clone K112-91, BD Biosciences), Alexa Fluor 555 anti-MYC (clone Y69 ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... GC B cells were enriched by CD43 (clone S7, BD Biosciences), IgD (clone 11-26c ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... with FACS-Diva software (BD Biosciences) and data were analyzed with FlowJo software (v10.8.1 ...
• CARs are organized in nanodomains in the plasma membrane of T cells that accumulate at tumor contact sites

  Christina Verbruggen, et al., bioRxiv - Immunology 2023

  Quote: ... Samples were measured on a FACS Canto II (BD Franklin Lakes, NJ, USA) or MACSQuant (Miltenyi Biotec ...
• CARs are organized in nanodomains in the plasma membrane of T cells that accumulate at tumor contact sites

  Christina Verbruggen, et al., bioRxiv - Immunology 2023

  Quote: ... Staining with 7-AAD (BD, Franklin Lakes, NJ, USA) was performed for live/dead cell discrimination as directed by the manufacturer ...
• Distinct Functional States of Neutrophils by Actin Disassembly and NF-kB/STAT3 Signaling

  Niko Heineken, et al., bioRxiv - Immunology 2023

  Quote: The proliferation of PBT was induced via CD3 antibody (20 ng/ml, clone: OKT3) and CD28 antibody (5 µg/ml, BD Biosciences 555725) bound to the surface of a 96-well Nunc MaxiSorpTM microtiter plate ...
• HIF-1α-mediated mitochondrial-glycolytic reprogramming controls the transition of precursor to terminally exhausted T cells

  Hao Wu, et al., bioRxiv - Immunology 2023

  Quote: ... All sample acquisition was performed with BD Celesta (BD Biosciences) or Attune Nxt (ThermoFischer Scientific ...
• HIF-1α-mediated mitochondrial-glycolytic reprogramming controls the transition of precursor to terminally exhausted T cells

  Hao Wu, et al., bioRxiv - Immunology 2023

  Quote: ... Medium was exchanged after 48 h of incubation and after 72 h cells were washed and permeabilized/fixed using Cytofix/Cytoperm kit (BD Biosciences). Cells were stained with VL-4 hybridoma cell line supernatant containing LCMV NP-specific antibodies for 60 min at RT ...
• HIF-1α-mediated mitochondrial-glycolytic reprogramming controls the transition of precursor to terminally exhausted T cells

  Hao Wu, et al., bioRxiv - Immunology 2023

  Quote: ... P14 T cells (Figure 5) were FACS-sorted from the spleen or LNs of chronically infected mice using a FACSAria III cell sorter (BD Biosciences). To multiplex different samples for scRNA sequencing ...
• Vγ9Vδ2 T cells recognize butyrophilin 2A1 and 3A1 heteromers

  Thomas S. Fulford, et al., bioRxiv - Immunology 2023

  Quote: ... or control PE-conjugated streptavidin (BD), for 30 min at 4°C ...
• Vγ9Vδ2 T cells recognize butyrophilin 2A1 and 3A1 heteromers

  Thomas S. Fulford, et al., bioRxiv - Immunology 2023

  Quote: ... All data were acquired on an LSRFortessa™ II (BD) and analyzed with FACSDiva and FlowJo (BD ...
• Dual role of Vascular Endothelial Growth Factor-C (VEGF-C) in post-stroke recovery

  Yun Hwa Choi, et al., bioRxiv - Immunology 2023

  Quote: ... Data was acquired using BD LSR II Flow Cytometer (BD Biosciences) and analyzed using FlowJo software.
• Upregulated Fcrl5 disrupts B cell anergy and causes autoimmunity

  Chisato Ono, et al., bioRxiv - Immunology 2023

  Quote: ... FO B (CD19+B220hiCD43− CD23+CD21lo) cells and ABCs (CD19+B220hiCD43−CD23−CD21loCD11b+CD11c+) were purified by FACS Melody (BD Biosciences) as shown in Fig ...
• Upregulated Fcrl5 disrupts B cell anergy and causes autoimmunity

  Chisato Ono, et al., bioRxiv - Immunology 2023

  Quote: ... TER-119 (TER-119) (all from BioLegend, BD Biosciences ...
• Macrophage Xanthine Oxidoreductase Links LPS Induced Lung Inflammatory Injury to NLRP3 Inflammasome Expression and Mitochondrial Respiration

  Mehdi A. Fini, et al., bioRxiv - Immunology 2023

  Quote: ... Control 293T cells and macrophage cells were lysed and immunoprecipitated with anti-IKKγ antibody (BD)(Figure S5B ...
• Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated T. gondii restriction in murine macrophages via vacuole nitration and intravacuolar network collapse

  Xiao-Yu Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... ⍺-iNOS (BD Biosciences, 610328, 1:500 dilution), ⍺- Ubiquitin (Enzo Life Sciences ...
• CCR9 overexpression promotes T-ALL progression by enhancing cholesterol biosynthesis

  Muhammad Jamal, et al., bioRxiv - Cancer Biology 2023

  Quote: ... DAPI (BD Pharmingen, USA).
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mCD41 (MWReg30, BD, 553848 or 562957), anti-mCD117 (c-Kit ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mCD90.2 (53-2.1, BD, 553004, or BioLegend, 140310), anti-mCD80 (16-10A1 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mSiglec-F (E50-2440, BD, 552126 or 564514), anti-mCD41 (MWReg30 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mGr-1 (RB6-8C5, eBioscience, 11-5931-82, or BD, 553124), anti- m/hTER119 (TER119 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD8a (1:200; BUV805; BD #612898), anti-CD103 (1:100 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... anti-F4/80 (1:100; BUV737; BD #749283), Ghost DyeTM Violet 510 (1:400 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were then permeabilized with BD Fix/Perm for 20 min at room temperature (RT) and stained intracellularly with antibodies in 1x Perm buffer (BD Biosciences): anti-IFN-γ (1:100 dilution ...
• Humoral correlates of protection against Mycobacterium tuberculosis following intravenous Bacille Calmette-Guérin vaccination in rhesus macaques

  Edward B. Irvine, et al., bioRxiv - Immunology 2023

  Quote: ... and 10μL GolgiStop (BD, 554724) were added to each well and incubated for 5hr at 37°C ...
• Humoral correlates of protection against Mycobacterium tuberculosis following intravenous Bacille Calmette-Guérin vaccination in rhesus macaques

  Edward B. Irvine, et al., bioRxiv - Immunology 2023

  Quote: ... Bead uptake was measured on a BD LSR II (BD Biosciences). Data were collected in FACSDiva (version 9.0 ...
• NK cell-induced damage to P.falciparum-infected erythrocytes requires ligand-specific recognition and releases parasitophorous vacuoles that are phagocytosed by monocytes in the presence of immune IgG

  Padmapriya Sekar, et al., bioRxiv - Immunology 2023

  Quote: ... stained with LIVE-DEAD stain and then flow cytometry was performed on them on LSRFortessa X20 cell analyzer (BD Biosciences), and data analyzed with FlowJo (FlowJo ...
• NK cell-induced damage to P.falciparum-infected erythrocytes requires ligand-specific recognition and releases parasitophorous vacuoles that are phagocytosed by monocytes in the presence of immune IgG

  Padmapriya Sekar, et al., bioRxiv - Immunology 2023

  Quote: ... Flow cytometry was performed on LSRFortessa X20 cell analyzer (BD Biosciences), and data analyzed with FlowJo (FlowJo ...
• Klebsiella pneumoniae Co-infection Leads to Fatal Pneumonia in SARS-CoV-2-infected Mice

  Crystal Villalva, et al., bioRxiv - Immunology 2023

  Quote: ... Bacteria were grown in nutrient broth (BD, Franklin Lakes, NJ) to an exponential growth curve (OD600 = 0.6) ...
• Anti-PD-1-iRGD Peptide Conjugate Boosts Antitumor Efficacy via Engagement Augmentation and Penetration Enhancement of T cells

  Yunfeng Pan, et al., bioRxiv - Immunology 2023

  Quote: ... CD45+ Cell sorting was performed using the FACS Aria II (BD Biosciences) system.