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• ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion

  Demis Menolfi, et al., bioRxiv - Genetics 2023

  Quote: ... Flow cytometry was performed on a FACSCalibur flow cytometer (BD Biosciences) or Attune NxT cytometer (ThermoFisher Scientific) ...
• G1/S Boundary Activates Interferon and Inflammatory Response Genes

  Gözde Büyükkahraman, Tae Hoon Kim, bioRxiv - Genomics 2023

  Quote: ... cells death was measured with PE Annexin-V (BD Biosciences) according to manufacturer’s instructions ...
• G1/S Boundary Activates Interferon and Inflammatory Response Genes

  Gözde Büyükkahraman, Tae Hoon Kim, bioRxiv - Genomics 2023

  Quote: ... Cells were stained with 7-AAD viability staining solution (BD Biosciences) and 60,000 live cells per condition were sorted by BDAria Fusion and FlowJo software.
• G1/S Boundary Activates Interferon and Inflammatory Response Genes

  Gözde Büyükkahraman, Tae Hoon Kim, bioRxiv - Genomics 2023

  Quote: ... Then the cells were strained and analyzed by BD LSRFortessa™ Flow Cytometer and FlowJo software.
• Structural Organization of the Retriever-CCC Endosomal Recycling Complex

  Daniel J. Boesch, et al., bioRxiv - Cell Biology 2023

  Quote: ... cells were fixed and permeabilized using BD Cytofix/Cytoperm solution kit according to the manufacturer’s instructions (BD Biosciences). Thereafter ...
• Functional glycoproteomics by integrated network assembly and partitioning

  Matthew E. Griffin, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-Nup62 (BD Biosciences ...
• Soluble LIGHT (TNFSF14) activates endothelial cells, thereby priming the first vessel-occlusive events in acute sickle cell disease

  Philippe Chadebech, et al., bioRxiv - Cell Biology 2023

  Quote: ... They were fixed by incubation for 15 min with 1X Cell FIX (BD Biosciences) and then analyzed on a FACS Canto II flow cytometer (BD).
• Metabolic regulation in erythroid differentiation by systemic ketogenesis in fasted mice

  Wenjuan Ma, et al., bioRxiv - Cell Biology 2023

  Quote: ... B220 (RA3-6B2, PerCP-Cy5.5; BD Biosciences), Gr-1 (RB6-8C5 ...
• Metabolic regulation in erythroid differentiation by systemic ketogenesis in fasted mice

  Wenjuan Ma, et al., bioRxiv - Cell Biology 2023

  Quote: ... CD8a (53-6.7, PerCP-Cy5.5; BD Biosciences), B220 (RA3-6B2 ...
• The aryl hydrocarbon receptor regulates epidermal differentiation through transient activation of TFAP2A

  Jos P.H. Smits, et al., bioRxiv - Cell Biology 2023

  Quote: ... were coated with rat tail collagen (100 µg/mL, BD Biosciences, Bedford, USA) at 4°C for 1 hour ...
• Vitamin C activates young LINE-1 elements in mouse embryonic stem cells via H3K9me3 demethylation

  Kevin C.L. Cheng, et al., bioRxiv - Genetics 2023

  Quote: ... Primed H9 cells were maintained in HFF-conditioned media (HFF-CM) supplemented with 8 ng/ml of β-FGF (MILTENYI BIOTE) on Matrigel (BD, Biosciences) coated surfaces cell lines in a humidified 5% CO2 incubator at 37ºC ...
• Scalable, cell type-selective, AAV-based in vivo CRISPR screening in the mouse brain

  Biswarathan Ramani, et al., bioRxiv - Genomics 2023

  Quote: ... sections were lastly incubated for 15 mins in Hoechst 33342 (BD Pharmingen, 561908) diluted 2 μg/ml in 1× PBS ...
• Multiplexed functional assessments of MYH7 variants in human cardiomyocytes at scale

  Clayton E. Friedman, et al., bioRxiv - Genetics 2023

  Quote: ... and the pellet was resuspended in 300 µL PBS with 5% FBS for flow cytometry using a CantoII (speed high; BD).
• Modelling Osteoarthritis pathogenesis through Mechanical Loading in an Osteochondral Unit-on-Chip

  Andrea Mainardi, et al., bioRxiv - Bioengineering 2023

  Quote: ... and GFP+ cells selected through FACS (BD FACSAria III Cell sorter). GFP+ hACs were subsequently re-passaged and used at passage 3.
• Versatile Tissue-Injectable Hydrogels with Extended Hydrolytic Release of Bioactive Protein Therapeutics

  Eric S. Nealy, et al., bioRxiv - Bioengineering 2023

  Quote: ... Cells were then washed 3x in flow cytometry stain buffer and fixed with 2% PFA for 20 minutes prior to acquisition on a LSRII Fortessa (BD Biosciences, San Jose, CA, USA). Samples were analyzed with FlowJo V10 software (Figure S4).
• A microfluidic model of colonocyte-microbiota interaction mimicking the colorectal cancer microenvironment

  Daniel Penarete-Acosta, et al., bioRxiv - Bioengineering 2023

  Quote: ... Feces were collected aseptically into anaerobic jars (BD Port-A-Cul™) and dissolved in anoxic PBS inside an anaerobic chamber ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-human IgG (G18-145) APC (BD, USA, Cat: #550931), anti-mouse CD3 APC (BioLegend ...
• Melatonin alleviates valproic acid-induced neural tube defects by modulating Src/PI3K/ERK signaling and oxidative stress

  Yuxiang Liang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and cell apoptosis in each group was detected by flow cytometry (BD Biosciences, San Jose, CA, USA).
• Evaluating cytotoxicity and genotoxicity of oil extracted from visceral fat of Caiman yacare (Daudin, 1802) in chinese hamster lung fibroblast in vitro

  Lucas P. Azevedo, et al., bioRxiv - Cell Biology 2023

  Quote: ... Measurements were recorded using an Accuri C6 plus flow cytometer (BD Biosciences), and event acquisition was performed using the FACSDiva program (BD Biosciences) ...
• Post-Transplant Administration of G-CSF Impedes Engraftment of Gene Edited Human Hematopoietic Stem Cells by Exacerbating the p53-Mediated DNA Damage Response

  Daisuke Araki, et al., bioRxiv - Cell Biology 2023

  Quote: ... BM and the spleen were assessed with the following antibody panel (all purchased from Becton Dickinson (BD), Franklin Lakes ...
• Post-Transplant Administration of G-CSF Impedes Engraftment of Gene Edited Human Hematopoietic Stem Cells by Exacerbating the p53-Mediated DNA Damage Response

  Daisuke Araki, et al., bioRxiv - Cell Biology 2023

  Quote: ... after which 1 uL each of CD45-PE (BD) and CD34-FITC (BD ...
• Post-Transplant Administration of G-CSF Impedes Engraftment of Gene Edited Human Hematopoietic Stem Cells by Exacerbating the p53-Mediated DNA Damage Response

  Daisuke Araki, et al., bioRxiv - Cell Biology 2023

  Quote: ... and CD34-FITC (BD) as well as 0.5 ug each of the 10 TotalSeq-B oligo-tagged antibodies (BioLegend ...
• Lysosomal Degradation Pathways Target Mutant Calreticulin and the Thrombopoietin Receptor in Myeloproliferative Neoplasms

  Amanpreet Kaur, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and PE conjugated anti-CD62p (BD Pharmingen 561921, 1:50 dilution) antibodies ...
• Lysosomal Degradation Pathways Target Mutant Calreticulin and the Thrombopoietin Receptor in Myeloproliferative Neoplasms

  Amanpreet Kaur, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Total MPL was then stained using a PE-conjugated anti-human MPL antibody (BD Pharmingen) in staining buffer with 0.2% saponin for 30 min at 4°C.
• GATA-regulated transcriptional program dictate cell fate equilibrium to establish the maternal-fetal exchange interface and fetal development

  Ananya Ghosh, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Red blood cell depletion from cell suspensions were performed using anti-Mouse Ter-119 antibody (BD Biosciences, Franklin Lake, NJ). Cellular viability was checked by automated cell counter (Countess 3 ...
• Comparative Analysis of Whole Transcriptome Single-Cell Sequencing Technologies in Complex Tissues

  Stefan Salcher, et al., bioRxiv - Cancer Biology 2023

  Quote: ... on ice and enzymatically digested with agitation for 30 minutes at 37°C using the BD TuDoR™ dissociation reagent (BD Biosciences). The obtained single-cell solution was sieved through a 70 µM cell strainer (Corning ...
• Comparative Analysis of Whole Transcriptome Single-Cell Sequencing Technologies in Complex Tissues

  Stefan Salcher, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The BD Single-Cell Multiplexing Kit (BD Biosciences) was used to combine and load two samples (tumor tissue and corresponding benign tissue ...
• Somatic mutation of Afadin leads to anchorage independent survival and metastatic growth of breast cancer through αE-catenin dependent destabilization of the adherens junction

  Max A.K. Ratze, et al., bioRxiv - Cancer Biology 2023

  Quote: ... E-cadherin (612130; 1:150; BD Biosciences), and GFP (SC-8334;1:150 ...
• Comparative Analysis of Whole Transcriptome Single-Cell Sequencing Technologies in Complex Tissues

  Stefan Salcher, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Cells were counted and viability assessed with the BD Rhapsody scRNA-seq platform (BD Biosciences) using Calcein-AM (Invitrogen ...
• Comparative Analysis of Whole Transcriptome Single-Cell Sequencing Technologies in Complex Tissues

  Stefan Salcher, et al., bioRxiv - Cancer Biology 2023

  Quote: ... FACS-sorted single-cells were immediately processed with the BD Rhapsody scRNA-seq platform (BD Bio-sciences). The BD Single-Cell Multiplexing Kit (BD Biosciences ...
• Comparative Analysis of Whole Transcriptome Single-Cell Sequencing Technologies in Complex Tissues

  Stefan Salcher, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and red blood cells were removed using the BD Pharm Lyse™ lysing solution (BD Biosciences). Cells were counted and viability assessed with the BD Rhapsody scRNA-seq platform (BD Biosciences ...
• Comparative Analysis of Whole Transcriptome Single-Cell Sequencing Technologies in Complex Tissues

  Stefan Salcher, et al., bioRxiv - Cancer Biology 2023

  Quote: ... was used to combine and load two samples (tumor tissue and corresponding benign tissue) onto a single BD Rhapsody cartridge (BD Bio-sciences). Sample-tag staining was performed according to the manufacturer’s protocol (sample-tag staining at room temperature for 20 minutes and washing by centrifugation at 400 g for 5 minutes) ...
• Selective Inhibition of Interleukin-2 Inducible T Cell Kinase (ITK) Enhances Anti-Tumor Immunity in Association with Th1-skewing, Cytotoxic T cell Activation, and Reduced T Cell Exhaustion

  Lih-Yun Hsu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... for 4-5 hr in the presence of Golgi Stop (1/1000, BD) and Golgi Plug (1/1000 ...
• Selective Inhibition of Interleukin-2 Inducible T Cell Kinase (ITK) Enhances Anti-Tumor Immunity in Association with Th1-skewing, Cytotoxic T cell Activation, and Reduced T Cell Exhaustion

  Lih-Yun Hsu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... TIGIT (1G9, BD), LAG3 (C9B7W ...
• Targeting BTN2A1 enhances Vγ9Vδ2 T cell effector functions and triggers tumor cells pyroptosis

  Anne Charlotte le Floch, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Supernatants were further analyzed for TNFα and/or IFNγ production using respectively BD OptEIA Human TNFα and IFNγ ELISA kits (BD Biosciences). Flat-bottom 96-well plates (COSTAR ...
• A CD24+CD271+ melanoma cancer stem cell generates a diffuse hierarchy of attributes that promote metastasis and therapeutic resistance

  Olivia Knowles, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD271-PerCP/Cy5.5 and CD271-APC (both clone C40-1457, BD Bioscience), and EpCAM-APC (clone HEA-125 ...
• Conditional lethality profiling reveals anticancer mechanisms of action and drug-nutrient interactions

  Kyle M. Flickinger, et al., bioRxiv - Cancer Biology 2023

  Quote: ... the population was single-cell FACS-sorted into 96-well plates containing RPMI11+2S (BD FACSMelody Cell Sorter). After 1.5-2 weeks ...
• “Isolation and characterization of a novel hormone receptor positive mammary adenocarcinoma MCa-P1362 with stromal drivers of tumor growth, metastasis, and drug resistance”

  Samir Jana, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Further analyses of cellular populations were performed using FlowJo software version 10 (BD, USA).
• “Isolation and characterization of a novel hormone receptor positive mammary adenocarcinoma MCa-P1362 with stromal drivers of tumor growth, metastasis, and drug resistance”

  Samir Jana, et al., bioRxiv - Cancer Biology 2023

  Quote: ... the cells were washed and analyzed by flow cytometry using a BD FACS Calibur (BD, USA). Further analyses of cellular populations were performed using FlowJo software version 10 (BD ...
• A high-content screen identified ingenol-3-angelate as an enhancer of B7-H3-CAR T cell activity by increasing B7-H3 protein expression on the target cell surface via PKCα activation

  Ha Won Lee, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Tumor cell lines were evaluated for expression of B7-H3 using B7-H3 antibody (clone 7-517, BD or clone FM276, Miltenyi).
• Multiple myeloma long-term survivors display sustained immune alterations decades after first line therapy

  Raphael Lutz, et al., bioRxiv - Cancer Biology 2023

  Quote: ... after implementation of the EuroFlow Standard Operating Protocol for Instrument Setup and Compensation in FACSDiva (BD Biosciences, San Jose, CA, USA). Final data analysis was performed in Infinicyt 2.0 (Cytognos ...
• A small molecule inhibitor of PTP1B and PTPN2 enhances T cell anti-tumor immunity

  Shuwei Liang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... PerCP-Cy5.5 or phycoerythrin-cyanine 7 (PE-Cy7)-conjugated CD4 (BD Biosciences Cat# 558170 ...
• A small molecule inhibitor of PTP1B and PTPN2 enhances T cell anti-tumor immunity

  Shuwei Liang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Invitrogen or eBioscience were used for flow cytometry: Phycoerythrin (PE) or peridinin chlorophyll protein -cyanine 5.5 (PerCP-Cy5.5)-conjugated CD3 (BD Biosciences Cat#5 61808 ...
• A small molecule inhibitor of PTP1B and PTPN2 enhances T cell anti-tumor immunity

  Shuwei Liang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... PerCP-Cy5.5 or phycoerythrin-cyanine 7 (PE-Cy7)-conjugated CD4 (BD Biosciences Cat# 558170; BD Biosciences Cat# 550954 ...
• A small molecule inhibitor of PTP1B and PTPN2 enhances T cell anti-tumor immunity

  Shuwei Liang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... PerCP-Cy5.5 or phycoerythrin-cyanine 7 (PE-Cy7)-conjugated CD4 (BD Biosciences Cat# 558170; BD Biosciences Cat# 550954, BD Biosciences Cat# 561099); Brilliant Violet (BV ...
• The tRNA Gm18 Methyltransferase TARBP1 Promotes Hepatocellular Carcinoma Progression via Metabolic Reprogramming of Glutamine

  Xiaoyan Shi, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and the apoptotic cells were detected by flow cytometry analysis (BD FACSCanto). For cell cycle analysis ...
• Targeting Ras signaling excitability in cancer cells through combined inhibition of FAK and PI3K

  Chao-Cheng Chen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and the data was analyzed with FlowJo v10 from BD Biosciences (Franklin Lakes ...
• Live-cell invasive phenotyping uncovers the ALK2/BMP6 iron homeostasis pathway as a therapeutic vulnerability in LKB1-mutant lung cancer

  Junghui Koo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... DNA content was measured by flow cytometry with FACSCalibur (BD Biosciences), and data were analyzed to determine the distribution of cells with sub-G1 ...
• Anti-CD117 CAR T cells incorporating a safety switch eradicate human acute myeloid leukemia and hematopoietic stem cells

  Chiara F. Magnani, et al., bioRxiv - Cancer Biology 2023

  Quote: ... followed by anti-CD117 antibody and sorted with a FACS Aria III (BD Biosciences).
• Anti-CD117 CAR T cells incorporating a safety switch eradicate human acute myeloid leukemia and hematopoietic stem cells

  Chiara F. Magnani, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Cells were acquired with an LSR II Fortessa cytometer (BD Bioscience, Franklin Lakes, NJ, USA) and the data were analyzed with FlowJo (version 10.0.8 ...