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Citations for Becton, Dickinson and Company :
7651 - 7700 of 8007 citations for 6 methyl N1 4 pyridin 3 yl thiazol 2 yl benzene 1 3 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were incubated overnight with different anti-aSyn primary antibodies with different binding properties: either 1:1000 mouse anti-aSyn (BD Biosciences), 1:3000 mouse anti-aggregated-aSyn (clone 5G4 ...
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bioRxiv - Bioengineering 2021Quote: ... We incubated the ECs in a 1:500 primary antibody solution containing Mouse anti-CD144 (VE-Cad, BD Pharmingen Cat. #55561) in ADB overnight at 4 oC ...
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bioRxiv - Cancer Biology 2021Quote: ... and cells incubated for 30 min on ice with following antibodies: PE-conjugated anti-CD11b (M1/70, 1:3000, BD Biosciences), APC-conjugated anti-F4/80 (for macrophages ...
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bioRxiv - Immunology 2020Quote: ... cells were prepared as described for flow cytometry and then sorted into FBS containing media (RPMI 1640, 20% FBS, 1% HEPES, 100 mg/mL penicillin/streptomycin) on a FACSAria II (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... for 10 min followed by staining with antibodies: 1 μl of PE-conjugated anti-mouse Ly-6G (BD Pharmingen, CA, USA), 1 μl of FITC-conjugated anti-mouse CD206 (Biolegend ...
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bioRxiv - Immunology 2020Quote: We centrifuged the samples and resuspended each in 1 mL of an antibody master mix consisting of Brilliant Buffer (BD Biosciences) and anti-CCR6 - PECy7 (BioLegend ...
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bioRxiv - Immunology 2019Quote: ... spinal cord tissue was homogenized with 1X HBSS in the presence of Golgi Plug (1:1000, BD Biosciences, San Jose, CA) and passed through nylon mesh filter for further dissociation ...
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bioRxiv - Molecular Biology 2019Quote: ... 20μg of total protein was loaded per lane and probed with the following primary antibodies dissolved in TBS-T: Topoisomerase IIβ (1:1000, overnight) (BD Biosciences), αTubulin (DMIA ...
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bioRxiv - Microbiology 2020Quote: ... xanthus cells were grown in liquid CYE medium (10 mM MOPS pH 7.6, 1% (w/v) Bacto™ casitone (BD Biosciences), 0.5% yeast extract and 4 mM MgSO4 ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were washed with PBS-T and incubated with the Alexa-fluor 647 cleaved PARP primary and Alexa-fluor 488 goat anti-rabbit secondary antibodies (1:500 dilution, BD Bioscience) overnight at room temperature ...
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bioRxiv - Immunology 2021Quote: Interleukin-8 secretion of treated THP-1 monocytes was quantified by the ELISA OptEIA™ Set for human interleukin-8 (BD) following the manufacturer’s protocol with optimized wash buffer composition (50x dilution) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pelleted and washed twice with 1% FBS in PBS and subjected to flow cytometry using a FACSMelodyTM (BD Biosciences).
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bioRxiv - Physiology 2021Quote: INS-1 cells were collected for flow cytometry analysis using FITC Annexin V apoptosis detection reagent (BD Biosciences, San Jose, CA). The apoptotic cells were measured by FACS Count™ Flow Cytometer (BD Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then blocked for 5 minutes at room temperature using anti-mouse CD16/32 Fc block (1/50) (BD Biosciences). Cells were then incubated with the staining (Ly6g-APCCy7 (1A8) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with 1 × PBS (30 s) and stained on ice in the dark for 30 min with anti-CD3 (BD, 557705), anti-CD4 (BioLegend ...
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bioRxiv - Plant Biology 2020Quote: ... and diluted to a final concentration of 1.5 × 105 spores ml−1 in half strength potato dextrose broth (PDB ½, BD Difco™) prior to inoculation ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 μL of the cell suspension (more than 1×105 cells) was transferred to a 5 mL FACS tube (BD Biosciences) and stained in duplicate with 10 μL of FITC Annexin V conjugate and 10 μL of propidium iodide (PI ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The sections were then blocked and permeabilized with 5% Donkey serum 0.01% Triton X-100 for 30 min at RT before being incubated with rabbit anti-YAP dilution 1/100 (Signalling Technology #14074) and mouse anti beta-catenin (BD 610153) (dilution 1/200 ...
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bioRxiv - Immunology 2021Quote: ... cells were previously stimulated with 100 ng/ml of PMA and 1 μg/ml of Ionomycin for 1h at 37°C and then incubated in GolgiStop and GolgiPlug (both from BD PharMingen) for 2 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: The following monoclonal antibodies (Abs) were used for flow cytometry and cell sorting: anti-c-Kit (2B8, BD Biosciences, 1:100), -Sca-1 (E13-161.7 ...
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bioRxiv - Developmental Biology 2021Quote: Mouse femur single cells were obtained as described above in the flow cytometry section and stained with biotin-conjugated antibodies for Lin (Ter119, Gr-1, B220, CD3e) (BD, 559971) followed by negative selection using streptavidin-coated magnet beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... bone marrow cells were subjected to red blood cell lysis and stained with fluorochrome-conjugated anti-mouse antibodies to CD11b (Mac-1, BD Biosciences) and/or Ly6G (1A8 ...
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bioRxiv - Cancer Biology 2021Quote: MART-1-specific T cells from uveal melanoma biopsies were identified as previously described (13) and sorted using FACSAria III (BD Biosciences). Sorted MART1-specific T cells were co-cultured with irradiated allogenic peripheral blood leukocytes at a 1:200 ratio in AIM-V cell culture medium (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB- 231-GFP cells (1 × 106) were resuspended in 100 μL of 20% of collagen I (BD Biosciences, cat #354234). Cells were injected in the 4th lower left mammary fat pad of the mouse ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were fixed in 1% formalin and analyzed on a BD FACS Canto II running the FACS Diva Software (BD Biosciences). Fluorescence values were normalized to an unstained control samples and initial compensation applied using IgG-APC control antibody (Miltenyi Biotech ...
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bioRxiv - Immunology 2021Quote: ... stained cells were resuspended in 1% paraformaldehyde (PFA) and acquired using Stained cells were acquired on a BD LSRFortessa (BD Biosciences) and analysis performed using FlowJo v10.0.8 (Tree Star ...
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bioRxiv - Immunology 2021Quote: ... and cell surface markers were stained with the following anti-mouse antibodies at 1:200 dilution per 106 cells: Ly6C (AL-21; BD Biosciences), Ly6G (1A8 ...
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bioRxiv - Immunology 2020Quote: ... spleen or lymph node cells in complete RMPI at a ratio of approximately 10 T cells to 1 DC in the presence of Golgi Plug (BD Bioscience). Co-cultures were incubated at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Sections were stained using the following antibodies: rat anti-mouse Ly-6C/Ly-6G (RB6-8C5, BD Bioscience, #550291, 1/200) followed by AlexaFluor647-conjugated goat anti-rat IgG (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... or with no target cells (or an E:T of 1:0) and cultured overnight before being staining with allophycocyanin (APC)-conjugated anti human-CD69 antibody (BD Biosciences #555533). Flow cytometry was performed using a BD- Fortessa (BD Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... To express CRE and induce recombination phleomycin resistant colonies were selected and grown overnight in 3ml of YP-Raffinose (1% w/v yeast extract (BD Biosciences), 2% w/v Bacto-peptone (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... splenocytes and tumor-infiltrating cells were cocultured with 1 μmol/L of AdpgkMut or AH1 peptide in the presence of Brefeldin A (BD Biosciences) for 5h in vitro ...
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bioRxiv - Immunology 2020Quote: For data acquisition on the BD FACSymphony the following amounts of 14 different fluorescent streptavidin conjugates were added to 10 μl of pMHC monomer (100 μg/ml): 1 μl of SA-BB790 (BD, custom), 1 μl of SA-BB630 (BD ...
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bioRxiv - Immunology 2021Quote: ... Resting- or in vitro-generated effector CD4+ or CD8+ T cells (105 cells/well) were stimulated with 1 μg/ml plate-bound anti-mouse CD3 mAb (BD Bioscience) in combination with different concentrations of P3CSK4 ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli strains were routinely grown aerobically at 37 °C under aerobic conditions with shaking at 1 × g in Luria-Bertani medium (BD, USA), supplemented with 25 μg mL−1 chloramphenicol (Merck ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed two times and resuspended in 1% paraformaldehyde/1x PBS for collection on a LSR II or FACSymphony flow cytometer (BD Biosciences). Data was analyzed in Flow Jo version 9.9.4.
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bioRxiv - Neuroscience 2022Quote: ... the cell suspension was then incubated on two PDGFRα plates coated with rat anti-PDGFRα IgG (BD Biosciences, 558774; 1:500). The adherent cells were washed with PBS to remove all antigen-negative nonadherent cells ...
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bioRxiv - Neuroscience 2022Quote: ... cells were fixed with IC fix (eBioscience88-8824-00) for 30 minutes followed by incubation with CD74 FITC 1:100 (BD 561941) in permeabilization buffer (eBioscience88-8824-00 ...
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bioRxiv - Cancer Biology 2022Quote: The fraction of CD44+/CD24− cells was determined by trypsinizing and resuspending cells in FACS buffer at 10×106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Cell Biology 2022Quote: For 3T3 NIH fibroblasts cells infected with C/EBPα-ER-IRES-hCD4 (WT and T35A) were incubated with 0.1µg per 1 million cells of both Fc block and hCD4-Biotin antibody (BD Pharmingen, Cat#555347) for 10 and 20 minutes respectively ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed twice with 1× PBS and flow cytometry analysis was performed on a FACS Canto II flow cytometer (BD Biosciences); data analysis was carried out using FlowJo software (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... were re-stimulated in vitro for 5h at 37°C 5% CO2 in cRPMI supplemented with GolgiStop (1/250; BD bioscience), GolgiPlug (1/500 ...
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bioRxiv - Immunology 2022Quote: ... was added and 0.6-1×106 lymphocyte-gated events were immediately acquired on an LSRII SORP (BD, San Diego, CA, USA).
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bioRxiv - Immunology 2022Quote: ... ELISAs were performed using paired rat anti-mouse IgE antibodies (capture with 1 μg/mL purified R35-72, BD Biosciences, detect with biotinylated R35-118, BD Biosciences) and streptavidin-HRP (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... and others were fixed using PBS containing 1% paraformaldehyde (Labsynth, Diadema, SP, Brazil) and acquired in FACS Canto II (BD Biosciences). Analyses were performed in FlowJo software (Becton Dickinson and Company).
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bioRxiv - Microbiology 2022Quote: ... xanthus cells were grown in liquid CYE medium (10 mM MOPS pH 7.6, 1% (w/v) Bacto™ casitone (BD Biosciences), 0.5% yeast extract and 8 mM MgSO4 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antibody bound to RBD in the presence of competitor was stained with Streptavidin-PE at a 1:1000 dilution (BD Biosciences) and analysed on a 200 Bioplex instruments ...
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bioRxiv - Microbiology 2022Quote: ... 50 μL of DEs were diluted into 500 μL of FACS diluent buffer (PBS + 1% Tween-20) in a 5 mL 12 × 75 mm round bottom FACS tube (BD Biosciences). After standard autocalibration on the Sony SH800 ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting pellet was resuspended in 1 ml of complete neurosphere medium containing epidermal growth factor (EGF, 20 ng/ml; receptor grade, BD Biosciences) and basic fibroblast growth factor (bFGF ...