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Citations for Becton, Dickinson and Company :
7551 - 7600 of 8007 citations for 6 methyl N1 4 pyridin 3 yl thiazol 2 yl benzene 1 3 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice with PBS and incubated for 20 minutes on ice with 1:100 dilution anti-CD16/32 (clone 2.4G2, BD biosciences) and 1:500 dilution Live/Dead nearIR dye (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by stimulation for the appropriate time at 37°C with pre-warmed anti-hamster IgG1 antibody (5µg ml-1; G94-56, BD Pharmingen). Ex vivo expansion of B cells was performed using recombinant murine IL-4 (100 u ml-1 ...
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bioRxiv - Immunology 2024Quote: ... MD4 and Ars/A1-derived antibody secreting cells were detected with Biotin-conjugated Mouse Anti-Mouse IgM[a] (Clone:DS-1; BD Biosciences), followed by Streptavidin-AP (SouthernBiotech) ...
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bioRxiv - Immunology 2024Quote: ... diluted with fluorescence-activated cell sorting (FACS) buffer (PBS containing 0.1% sodium azide and 1% bovine serum albumin) and Brilliant Stain Buffer (BD Biosciences). The cells were washed in FACS buffer and fixed in PBS containing 4% paraformaldehyde (Electron Microscopy Sciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... PDX derived cells were centrifuged and suspended in ALDEFLUOR assay buffer containing Fixable Viability Stain 660 (FVS660, 1:1000, BD Horizon) after incubation at 37°C ...
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bioRxiv - Immunology 2021Quote: ... for 5 minutes prior to staining at 4°C in the dark using the antibodies listed in supplementary table 1 and analysed using an LSR Fortessa cytometer (BD Biosciences) and FlowJo software (BD) ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight in blocking solution with the following primary antibodies (1:100): rat anti-mouse SiglecF (E50-2440, 552126 BD Biosciences), Armenian hamster anti-mouse CD80 (16-10A1 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with pS6 or HK2 antibody for 1 h and O-GlcNAc antibody for 20 min at RT in the dark in cytoplasmic permeabilization buffer (BD Biosciences). Nuclear permeabilization buffer was used for c-Myc staining ...
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bioRxiv - Immunology 2021Quote: ... 10% FCS and incubated with the indicated pool of peptides (5 μg/ml final concentration of each peptide) and brefeldin A (1 μg/ml; BD Pharmingen) at 37°C for 12-16 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... run on PAGE together with input sample (1:20 of amount of immunoprecipitated proteins) and blotted with anti-PARP1 (Mouse Mab 551025 BD Pharmigen), anti-TRF1 (Rabbit Pab sc-6165 ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed once with FACS buffer and then stained with surface antibodies diluted 1:200 in Brilliant buffer (BD Biosciences) for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were re-suspended in 100 μl of FCSB and 1 μl of mouse anti-human CD10-PE (561002, BD Biosciences) and mouse anti-human CD92-AF647 (565316 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Three stable cell lines expressing shRNAs were cultured simultaneously under 1% hypoxia and 21% normoxia for 48 hours and collected for FITC-conjugated annexin V (BD Pharmingen) staining followed by apoptotic cell number analysis by flow cytometry.
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bioRxiv - Immunology 2022Quote: BMDMs were harvested as described before and Fc receptors were blocked with an anti-mouse CD16/CD32 antibody (1:250, BD Biosciences) in FACS buffer (5 % heat-inactivated FCS ...
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bioRxiv - Neuroscience 2022Quote: Dissociated and resuspended iTF-Microglia were blocked for 15 min with 1:20 Human FC Block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE/Cy7 anti-human CD184 (CXCR4 ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... and with hematopoietic stem cell markers APC-conjugated anti-CD117 (c-Kit) and PE-conjugated Ly6A/E (Sca-1) (BD Biosciences). Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μl of platelet suspension was mixed with 40 μl of staining solution (HEPES-Tyrode buffer supplemented with 2.5 mM CaCl2, 1:50 PE rat anti-mouse CD41 antibody (BD Biosciences 561850), and 3:50 FITC Annexin V (BD Biosciences 556419)) ...
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bioRxiv - Cell Biology 2022Quote: ... were loaded onto SDS-PAGE gel and immunoblotted using antibodies against clathrin heavy chain (1:1,000; BD Bioscience, San Jose, CA), dynamin (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were re-stimulated with 1 µg/mL of master peptide pools in the presence of brefeldin A (GolgiPlug; BD Bioscience) and monensin (GolgiStop ...
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bioRxiv - Immunology 2022Quote: ... NK cells were added to the washed ELISA plate and incubated together with anti-human CD107a (BD, 1:40, clone: H4A3), brefeldin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... stained with anti-CD117-PECy7 mAb (5 μg.mL−1) and viability dye and sorted by a FACSMelody™ cell sorter (BD biosciences). For immunofluorescence experiments ...
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bioRxiv - Immunology 2021Quote: ... TNFα and CD107a expression by multicolor flow cytometry panel (all antibodies used are listed in Table 1) in a BD LSR Fortessa Instrument (BD Biosciences). Polyfunctionality was evaluated using Boolean gating obtained with FlowJo v10 software.
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bioRxiv - Cell Biology 2019Quote: ... transferred to flow cytometry tubes and stained with 1:100 mouse IgG2a CD31-647 clone M89D3 (BD Bioscience, 558094, lot 8145771) for 30 min on ice then washed once with DPBS ...
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bioRxiv - Cell Biology 2019Quote: ... Cells expressing desired transgenes were isolated by culturing in growth media supplemented with puromycin (1 μg/mL) or using fluorescence-activated cell sorting (FACS) as appropriate (FACSAria2 or FACSAria3; BD Biosciences).
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bioRxiv - Cell Biology 2019Quote: ... Tet1KO and WT reprogramming intermediates were incubated 30 min on ice with Phycoerythrin (PE)-conjugated Mouse anti SSEA1 (1:100, Cat.#FAB2155P-025, R&D) and analysed on CANTO I (BD Biosciences). PGCLCs at day8 were sorted using FITC Mouse anti-SSEA1 (1:20 ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were resuspended with the 1× Binding Buffer and incubated with 5μL FITC-conjugated annexin V Annexin FITC (BD Biosciences, USA) and 5μL PI (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: Lymphocytes or purified splenic B cells were washed in PBS with 1% BSA and incubated with 1mg/ml purified rat anti-mouse CD16/CD32 (Fc block, BD Pharmingen) at 4°C for 15 min ...
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bioRxiv - Immunology 2019Quote: ... incubated with anti-mouse IgG1-fluorescein isothiocyanate for 45 min at room temperature (1:100 dilution; BD Pharmingen; cat. no. 553443), and washed three times with PBS ...
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bioRxiv - Immunology 2019Quote: ... cells were cultured in the presence of 1 μg/mL Brefeldin A for indicated timepoints and were fixed and permeabilized with Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei enriched in Nuclei Suspension Buffer were stained with DAPI (1:1000) for nuclei isolation using the FACSAria™ III cell sorter (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... The cells were then aliquoted into FACS tubes (0.25-1×106 cells/tube) with strainer caps (BD Biosciences, San Jose, CA).
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bioRxiv - Cancer Biology 2019Quote: ... Apoptosis in cell lines and CLL cells was measured by Annexin staining and flow cytometry using the FITC Annexin V Apoptosis Detection Kit 1 (BD Pharmingen). Percentage apoptosis represents the % of apoptotic cells above the % apoptosis in control cells ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were then incubated with fluorophore conjugated primary antibodies for 1 hr in the dark (Alexa 488 Vimentin Antibody (BD, #562338) and Alexa 647 E-Cadherin Antibody (BD ...
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bioRxiv - Developmental Biology 2019Quote: ... The precipitated cells were washed two times with 2% FBS/PBS and finally resuspended in 1% FBS/PBS for sorting of the GFP-positive PGCs by using a flow cytometry (FACSVerse, BD Biosciences). The GFP-negative somatic cells and GFP-positive PGCs after sorting were subject to evaluation of mutation efficiencies.
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bioRxiv - Microbiology 2019Quote: ... Cells were then washed three times in PBS followed by incubation in 1% Human Fc Block (BD Biosciences, San Jose, CA) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... cells were stained with a 1:50 dilution of phycoerythrin (PE)-conjugated mouse monoclonal antibody against human TRAIL (550516, BD Biosciences). For immunophenotyping of MSCs the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770 ...
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bioRxiv - Immunology 2021Quote: ... labelled with anti-human myeloid and stromal markers (Table 1) and analysed by flow cytometry on a LSR Fortessa (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Total T cells (1 × 106) were activated with plate bound α-CD3ε (OKT3, Biolgend) plus α-CD28 (CD28.2 RUO, BD Biosciences) at 5µg/mL each or corresponding isotype controls for a total of 72 hours ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with a panel of antibodies (Supplementary Table 1) in flow cytometry staining buffer with BD Horizon Brilliant Stain Buffer Plus (BD Biosciences) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed in flow buffer and fixed in flow buffer plus 1% PFA before filtering through 40 μM mesh strainer/tube (BD Biosciences). Flow cytometry was performed on the BD FACSCelesta cytometer and data was analyzed using FlowJo Version 10 (Becton ...
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bioRxiv - Neuroscience 2021Quote: ... Fc receptors were blocked as described above and cells were stained with CD3e FITC (1:200, clone 145-2C11, BD Pharmingen) or CD3e PE-Cy7 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... The pellet was resuspended in 1 ml of FACS buffer and analysis was performed on a LSRII-U flow cytometer (BD Biosciences). Single color controls were used to determine proper gating prior to sample analysis.
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bioRxiv - Neuroscience 2021Quote: ... Tissues were blocked with 5% donkey serum and 0.1% Triton X-100 in PBS for one hour at room temperature and incubated with 1:1000 of anti-CD47 (BD Biosciences), 1:500 of anti-TSP1 (Abcam) ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used for analysis of the H2B-GFP LR population (Figure 5): rat anti- Cd74-BUV395 (Clone In-1, BD, 740274), rat anti-Cd45-BUV563 (Clone 30-F11 ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interest were visualized after incubation with primary antibodies (α-synuclein 1:1000 BD Biosciences (San Jose, CA, USA) #610787 ...
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bioRxiv - Plant Biology 2021Quote: ... and diluted to a final concentration of 1.5 x 105 spores ml−1 in half strength potato dextrose broth (PDB ½, BD Difco™) prior to the inoculation ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were incubated for 15 minutes at room temperature in the dark in 50uL PBS with Fc Block (1/100 dilution, BD, 564220) and Zombie UV dye (1 in 500 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... at 1:1 E:T ratio at 37°C with 5% CO2 in the presence of a CD107a antibody for 1 hr before treatment with brefeldin A (10 µg/ml; BD Biosciences) and monensin (0.7µg/ml ...
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bioRxiv - Microbiology 2020Quote: ... red blood cells in a concentration of 1 × 107 cells/mL were incubated for half an hour with 100 μl thiazole orange (TO) (Retic-Count Reagent; BD Biosciences) and Alexa-647 conjugated mouse mAb against transferrin receptor (CD71 ...
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bioRxiv - Microbiology 2020Quote: ... and combined 1:1 with Tris-Borate-EDTA buffer (TBE) at a final working concentration of 1X in 50 mL conical tubes (BD Falcon). Samples (1.0 mL in 50 mL conical tubes ...