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Citations for Becton, Dickinson and Company :
7551 - 7600 of 7602 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA,and BD OptEIA Substrate Solution, BD Biosciences, for Spike ELISA) for 5 min ...
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bioRxiv - Immunology 2022Quote: Total white blood cells from 1-2 mL freshly collected blood in EDTA tubes were enriched through ammonium chloride lysis (BD Bioscience, catalog no. 555899), followed by centrifugation (300x g for 10min) ...
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bioRxiv - Cell Biology 2019Quote: ... The following day they were probed with antibodies to FLAP (Novus, IMG 3160, 1:100) and to 5-LO (BD Biosciences, 610695, RRID: AB_398018). Secondary antibodies were applied at 3 µg/mL for 1 h ...
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bioRxiv - Genetics 2020Quote: ... Cells were stimulated at 1×106 cells/ml with 10 μg/mL soluble Leishmania antigen (SLA) plus 1 μg/mL purified NA/LE anti-human CD28 (clone CD28.2, BD Biosciences, San Jose, CA, USA) at 37°C / 5% CO2 for 15 h and subsequently incubated in the presence of brefeldin A (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) strain harboring pCOLD-1-EhPTEN1 was grown at 37°C in 50 ml of LB medium (BD Difco, New Jersey, USA) in the presence of 100 µg/ml ampicillin ...
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bioRxiv - Cancer Biology 2021Quote: ... or 4T1.2 cells (1⨯106 per fat pad) in 50 μL of 50:50 (v/v) PBS:Matrigel™ (BD Biosciences, San Jose, CA, USA). Tumor growth was measured twice weekly by caliper measurements and weekly bioluminescent imaging (125 mg/kg luciferin I.P. ...
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bioRxiv - Microbiology 2019Quote: ... The mixture was filtered (Whatman #1) from which 10mL of the filtrate aliquoted into 14mL BD Falcon™ test tubes (BD Biosciences, Bedford, USA) and labeled accordingly ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... cells were centrifuged and resulting pellets were resuspended in 1:100 mixture of panel of antibodies: FITC-conjugated CD31 (BD Biosciences, Mississauga, ON, Canada), AF647-conjugated CD45 (Southern Biotech ...
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bioRxiv - Cell Biology 2020Quote: ... re-suspended in αMEM (with 10% FBS and 1% anti-mycotic) and then processed on an Aria II or Influx flow cytometer (BD Biosciences, San Jose, CA) to separate tdTomato-expressing (tdTom+ ...
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bioRxiv - Immunology 2020Quote: ... LN staining: macrophages were detected using a primary antibody against CD11b (1/1000, rat anti mouse CD11b conjugated with PE; BD Biosciences, San Jose, California), B-cell follicles were identified using rat anti mouse B220 Alexa F647 (1/1000 ...
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bioRxiv - Genomics 2021Quote: ... FCM were performed on 50 μl sample in triplicate at a pre-set flow rate of 66 μl.min-1 using a BD Accuri® C6 flow cytometer (BD Accuri® cytometers, Belgium) which is equipped with a 50mW solid state laser emitting light at a fixed wavelength of 488 nm ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in 100 μl of staining solution of FACS buffer containing biotinylated antibodies against CD31 (BD Biosciences, 558737, 1:100), CD45 (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained with Fixable Viability Dye eFluor 506 (1:1000 in PBS, 65-0866-14) and later CD14-APC (555399, BD Biosciences, Franklin Lakes, USA) and CD16-FITC (555406 ...
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bioRxiv - Genomics 2022Quote: The collected cells were first stained with live/dead aqua to exclude non-viable cells from the analysis and stained with fluorophore-conjugated antibodies against surface markers anti-CD45-BV786 (BD Bioscience Clone: OX-1), anti-CD11b-V450(BD Bioscience Clone ...
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bioRxiv - Neuroscience 2022Quote: ... approximately 1-2 mm apart along the same bundle of muscle fibers with the help of a 20G syringe tip (BD Microlance, Thermo Fisher Scientific). After the dental cement hardened ...
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bioRxiv - Immunology 2022Quote: ... 1.5 µg/mL) [31] in the presence of anti-CD28 and anti-CD49d antibodies (both at 1 μg/ml, BD, Franklin Lakes, New Jersey) and brefeldin-A (10 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... Purified naïve CD4+ T cells were stimulated with 2.5 μg/ml of anti-CD3e (#145-2C11, Cytek Biosciences, Fremont, CA) and 1 μg/ml of anti-CD28 (#553294, BD Biosciences, Franklin Lakes, NJ) antibodies in different culture conditions ...
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bioRxiv - Evolutionary Biology 2023Quote: Fecal samples of captive great apes from the Columbus and Houston Zoos (Supplemental Table 1) were collected using the BBL™ CultureSwab Collection and Transport System (BD Biosciences, NJ, USA), and shipped overnight at ambient temperature to our research laboratory where they were stored at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... spun for 10 minutes and then resuspended in 50 μL of a 1:200 dilution of Fc block (CD16/CD32, clone 2.4G2, BD Biosciences, cat. 553141, 0.5mg/mL) in FACS buffer (PBS ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: pCAF sorting based on TNFRSF12A protein level: 106 cells were incubated with BD Horizon BV421 anti-TNFRSF12A antibody (1/140, 565712 BD Biosciences, Franklin Lakes, NJ) and human TruStain FcXTM (1/200 ...
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bioRxiv - Developmental Biology 2023Quote: ... before the cell pellet was resuspended in 100µl Fc block (mouse BD Fc Block CD16/CD32 Clone 2.4G2, Cat#: 553141, BD Pharminogen, 1:100 in FACS buffer) and incubated on ice for 10 min ...
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bioRxiv - Cell Biology 2023Quote: Immunofluorescence staining was performed using heat antigen-retrieval in citric acid buffer (pH 6.0) and staining with E-Cadherin (BD Biosciences 610182, 1:1000 dilution). 5-ethynyl-2′-deoxyuridine (EdU ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were counter-stained with DAPI at a final concentration of 1 ng/mL and analyzed on a BD LSR Fortessa instrument (BD Biosciences, Franklin Lakes, NJ). The resulting data was processed using the software FlowJo (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... The following surface antibodies were used at the manufacturer recommended dilution of 0.25 μg antibody per million cells with the exception of CD57 APC which was tittered down 1/5 in order to be on scale: HLA-DR BUV395 (BD Biosciences, Clone: G46-6), NKp46 BV650 (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed with phosphate buffer three times for 10 minutes each and incubated with primary antibody (rat anti-mouse CD31, BD Biosciences, 550274, 1:100) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The ELISPOT plate was washed five times with PBST and 50 μl of 1 μg/ml of the anti-mouse IFNγ monoclonal detection antibody conjugated to biotin (BD Biosciences; cat. no. 551506) in PBS was added and allowed to incubate at room temperature for 2 hours ...
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bioRxiv - Molecular Biology 2019Quote: Both CVL-derived and dendritic cell LK23-derived EVs were diluted 1:1000 and incubated on ExoView (NanoView Biosciences, Brighton, MA) chips that were printed with anti-CD63 (BD Biosciences, Bedford, MA. Cat#: 556019) and anti-CD81 (BD Biosciences ...
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bioRxiv - Microbiology 2019Quote: Cryptococcus neoformans strains (Supplementary Table 8) were usually grown in liquid Yeast Peptone Dextrose (YPD: 1% yeast extract (BD Difco, Le Pont de Claix, France), 2% peptone (BD Difco) ...
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bioRxiv - Biochemistry 2020Quote: ... a mouse mAb specific for the neo-epitope generated at the N-terminal of the 89 kD PARP1 fragment formed after cleavage by apoptotic caspases between Asp214/Gly215 (clone F21-852, BD Biosciences #552596, 1:1000 dilution). Secondary detection was with HRP-conjugated goat anti-mouse IgG or donkey anti-rabbit IgG (BioLegend ...
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bioRxiv - Biochemistry 2020Quote: ... an affinity-purified mouse mAb that recognizes an epitope in the C-terminal NAD binding and catalytic domain of human PARP1 (clone 7D3-6, BD Biosciences #556493; 1:500 dilution); anti-cc-PARP1 ...
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bioRxiv - Microbiology 2021Quote: PHB21 (1 × 109 PFU) was spotted on a lawn of MRSA strain SA14 on tryptic soy agar (TSA) plates (Becton, Dickinson and Company, MD, USA) and incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... between 500.000 and 1 million GFP-mCherry double positive cells were sorted each day for 5 consecutive days using two cell sorters (BD FACSAriaII SORP and BD FACSAriaIII). Sorted cells were pooled ...
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bioRxiv - Cancer Biology 2021Quote: ... a total of 1 x 106 cells was injected in a ratio of 10:1 Cre+:reporter+ cells in sterile PBS with 50 % growth factor-reduced Matrigel (BD Biosciences, Franklin Lakes, NJ, USA).
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bioRxiv - Neuroscience 2020Quote: ... After a second digestion of the retina-RPE complex in papain (20 U/ml) for ten minutes at 37°C the RPE cells were carefully peeled off and plated on Matrigel (BD Biosciences; 1:80 in DMEM)-coated glass coverslips in ‘Miller’ medium [DMEM supplemented with 5 or 20% fetal bovine serum (v/v) ...
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bioRxiv - Cancer Biology 2019Quote: ... and cells in the S phase using incubation with bromodeoxyuridine (BrdU) for 1 h and labeling with an anti-BrdU antibody (APC BrdU flow kit, BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Young leaves65 of 6-week old wild-type Col-0 Arabidopsis plants in vegetative stage were selected to be fully infiltrated (approx. vol. 40 μL) with a 1 mL needleless syringe (BD, cat. no. 14-823-434) loaded with water ...
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bioRxiv - Immunology 2020Quote: ... then cell sediments were suspended in 1% paraformaldehyde buffer (300ul) and acquired on a on a Fluorescence Activated Cell Sorter (FACS) Calibur flow cytometer (BD Biosciences, San Jose, CA, USA). All samples acquired in this study were compensated using the single-color stained cells ...
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bioRxiv - Immunology 2020Quote: ... The purified CD4+ T cells were seeded at 1 × 105 cells/well in the anti-CD3 monoclonal antibody (mAb) (10 μg/mL, BD Pharmingen, Franklin Lakes, NJ, USA) precoated 96-well tissue culture-treated plate ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-positive cells among living cells were sorted and plated as 1 cell/well into 96-well plates using FACS Aria III (BD Bioscience, Franklin Lakes, NJ, USA). When clones reached 80% confluency ...
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bioRxiv - Microbiology 2021Quote: The following mouse and human monoclonal antibodies were utilized for virion capture assays: anti-PSGL-1 and anti-mouse IgG (BD Biosciences, Cat# 556053 and 557273) and anti-gp120s (clones 2G12 and PG9 acquired from the NIH ARP ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (BD Pharmingen 553673 2 μg/ml) at 4 °C for 20 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Fresh cultures of bacteria were prepared from frozen stocks by streaking onto gonococcal agar (GCA) consisting of gonococcal (GC) agar base supplemented with 1% v/v IsoVitaleX (BD Biosciences, Franklin Lakes, NJ, USA). On the following day ...
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bioRxiv - Immunology 2023Quote: ... 100 µL of whole blood was added to an antibody cocktail containing the following antibodies: mouse-anti-CD3-V500 (BD, catalog #560770, dil 1:30), mouse-anti-CD4-PerCP/Cy5.5 (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: Fresh cultures of different Gc strains were prepared from frozen stocks by streaking onto gonococcal agar (GCA) consisting of agar base supplemented with 1% v/v IsoVitaleX (BD Biosciences, Franklin Lakes, NJ, USA). On the following day ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 × 106 cell/mL cells were stained with the appropriate antibodies and analyzed by flow cytometry (BD FACS Fortessa, BD Biosciences, San Jose, CA, USA). Fluorescence minus one (FMOs ...
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bioRxiv - Genomics 2023Quote: ... 2.5 × 105 cells were stained for 75 minutes at room temperature with PE Mouse anti-PDX1 Clone-658A5 (BD Biosciences; Catalog no. 562161; 1:10) and Alexa Fluor® 647 Mouse anti-NKX6.1 Clone R11-560 (BD Bioscience ...
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bioRxiv - Immunology 2024Quote: ... Supernatant from tissue lysate was diluted 1:10 and loaded onto BD Cytometric Bead Array Mouse IFNg Flex set (BD Biosciences, Franklin Lakes, NJ, USA), used as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The amount of coating gp120 was optimized through competition by binding of the Leu3A (anti-CD4) antibody (clone SK3; Catalog no. 340133; Final dilution 1:5; BD Bioscience, San Jose, CA, USA). Cryopreserved peripheral blood mononuclear cells ...
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bioRxiv - Synthetic Biology 2019Quote: ... with 2% (w/w) PEG-PFPE amphiphilic block copolymer surfactant (Ran Biotechnologies, 008-FluoroSurfactant) were loaded into separate syringes (BD, 1 mL and 5 mL, respectively) and injected with 23 gauge needles through PTFE tubing (0.022” ID ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, for the RBD ELISA, and BD OptEIA Substrate Solution, BD Biosciences, for the spike ELISA) for 5 minutes ...