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Citations for Becton, Dickinson and Company :
7451 - 7500 of 7602 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Spleens were harvested and single-cell suspensions were stained with B220 conjugated to fluorescein isothiocyanate (B220-FITC, BD Biosciences, 1:500 dilution), Fas conjugated to PE-cyanine 7 (Fas-PE-Cy7 ...
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bioRxiv - Immunology 2023Quote: ... and plated in round-bottom 96-well plates at 1-2 x 106 cells per well in complete RPMI (cRPMI) medium containing CD28 costimulatory antibody (BD Biosciences #553294) and brefeldin A ...
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bioRxiv - Immunology 2022Quote: ... with the following antibodies/reagents (all at 1:200 dilution) for 30 min on ice: anti-CD20-PE-Cy7 (BD Biosciences, 335828), anti-CD14-APC-eFluor 780 (Thermo Fischer Scientific ...
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bioRxiv - Genetics 2023Quote: Fluorescently conjugated antibodies for cell surface staining (Supplementary Table 1) were prepared in Brilliant Stain Buffer (Cat. no. 556349, BD Biosciences, Australia) and incubated with cells in a 96 well plate (Cat ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then stained at a 1:50 dilution with antibodies to immune cell targets as follows: CD3- FITC (BD Biosciences, 555332), CD8-PE (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and resuspended in flow buffer containing 1% FA to be analyzed using the LSR Fortessa (BD Bioscience, USA). Data were analyzed using FlowJo 10 software (FlowJo LLC ...
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bioRxiv - Systems Biology 2023Quote: ... all strains are grown in YPD (2% w/v Bacto Peptone, BD Biosciences #211677; 2% w/v D-glucose, BD Biosciences #215520; 1% w/v Yeast Extract, BD Biosciences #212750) at 30°C.
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... Permeabilization was followed by blocking cells using 5% BSA in HBSS+ for 30 min and incubated with the primary antibody solution - Mouse anti-human CD144 (1:150; 555661, BD Biosciences, USA) for overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and stained for the following surface markers on ice for 30 min: CD4 (APC-Cy7, 1:100, clone RM4-5, BD Biosciences, 565650), CD8b (Alexa Fluor 488 ...
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bioRxiv - Cell Biology 2023Quote: ... plated at a density of 1 X 106 cells/well in a Matrigel-coated 6 well plates (BD, 354277; Fisher Scientific, 140675). For the first 24 hours after passaging ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked with 5% skimmed milk in TBS-Tween and incubated with anti-αSyn clone 42 (BD Biosciences, 1:1,000 dilution). In a slot blot ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated with anti-mouse CD32 for 5 min at 4°C to block Fc receptors and stained with fluorochrome conjugated antibodies to CD45R/B220 PE (1:200) (clone RA3-6B2, BD Bioscience #553089), Ter119 PE (1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed with PBS+5% FBS and stained with 1:100-dilutions of AF647-conjugated anti-PAX-6 (BD Biosciences; 562249) and PerCP-Cy5.5-conjugated anti-SOX-2 (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... The urine samples were obtained in sterile BD Vacutainer® urine C&S Preservative Tubes (BD, 1 Becton Drive, Franklin Lakes. NJ). Specifically ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were then incubated for 1 h with primary antibodies directed against the cis-Golgi marker GM130 (mouse anti-GM130, BD bioscience, 610822) and/or trans-Golgi marker TGN46 (sheep anti-TGN46 ...
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bioRxiv - Immunology 2024Quote: ... samples were washed with 1X PBS and fixed in 1% formaldehyde before acquisition on a FACSCanto™ II (Beckmann Coulter) using the FACS DiVa software (BD, Biosciences). Also ...
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bioRxiv - Immunology 2024Quote: ... Stained samples were fixed in 1% formaldehyde and acquired on FACSCanto™ II (Beckmann Coulter) using the FACS DiVa software (BD, Biosciences). Data were analysed using FlowJo v10.6 (Tree Star Inc) ...
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bioRxiv - Cell Biology 2024Quote: ... They were stained for 2 hours at room temp with the following primary antibodies: mouse anti-GM130 (1:250, BD Bioscience, BDB610822) and rabbit anti-TGN46 (1:500 ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 ml of cell suspension was incubated with 2 μl of Nile red solution (0.25 mg ml−1) for 5 minutes before the analysis with a bench flow cytometer (BD Accuri C6, BD Biosciences). To determine Nile red fluorescence ...
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bioRxiv - Cancer Biology 2021Quote: ... PE mouse anti-human VCAM-1 or Alexa Fluor 647 mouse anti-human CD31 (all antibodies were from BD, Franklin Lakes, NJ, USA). Samples were analyzed on Gallios Flow Cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... The antibodies used for the flow cytometry analysis of the cell surface markers were: anti-human CD11b (740965, BD OptiBuild™, 1:100), anti-human CD14 (11-0149-42 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with a fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD31 antibody (1:50; BD Pharmingen, San Diego, CA, USA) for 30 min at room temperature followed by 3 washes with phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... Microglia were washed 3x with PBS before incubating them in iTF-Microglia media supplemented with 1:2000 GolgiPlug™ (BD; Cat. No. 555029) for 4h ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated for 60 minutes in 1% BSA and 5% goat serum in PBS and then incubated overnight with anti-p21 antibody (BD Pharmingen, mouse, #556431) and anti-p16 antibody (abbiotec ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×106 cells were injected in the right shoulder subcutaneously in 150 μL volume of 50% media/matrigel (BD Biosciences, San Jose, CA) and allowed to grow for approximately two weeks until the tumors reached about 8 mm in diameter (100 ± 8 mm3).
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bioRxiv - Developmental Biology 2019Quote: Isolated cells from E18.5 Wnt1-Cre;Ift88fl/+;R26mT/mGand Wnt1-Cre;Ift88fl/fl;R26mT/mG embryonic scleras were incubated 45 min with an anti-CD31 Ab (BD Biosciences, 1:500), washed ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then washed twice with PBS + 1% BSA before analysis with a BD LSR II Flow Cytometer (BD Biosciences, San Jose, CA).
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bioRxiv - Pharmacology and Toxicology 2019Quote: The seeded cells (5 x 104 cells/mL) were pre-stained with 1 μL Orange NO probe (BD Biosciences, San Jose, CA, USA) per 500 μL cell suspension for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: The concentration of prey and THP-1 cells were measured prior to phagocytosis by flow cytometry (Accuri C6, BD or CytoFLEX, Beckman-Coulter), if needed ...
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bioRxiv - Microbiology 2021Quote: ... the cryosections were incubated for 1 h in the blocking solution (PBS containing 5% goat serum and 5 μg/mL mouse BD Fc Block™) and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... 100 μl of the bacterial inoculum was transferred from a Hungate tube to under oil in the eppendorf tube using a syringe (1 ml, Luer-lok Tip, BD Biosciences, REF 309628). Bacteria were added to the media above the colon monolayer by pipette at 1:20 v/v ratio (2 μl bacteria:40 μl media ...
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bioRxiv - Cell Biology 2021Quote: ... cells were rinsed twice in sort medium before being stained with Propidium Iodide (PI, 1:100) to stain dead cells and sorted with a FACSAria III flow cytometer (BD Biosciences, Heidelberg, Germany) as previously described (Cesetti et al. ...
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bioRxiv - Microbiology 2021Quote: ... were anesthetized and a sterile 1-cm long catheter segment (Qosina) inserted into the peritoneum using an 18-gauge BD Spinal needle/stylette (Becton, Dickinson and Company,). Three hours post-catheter implantation ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for ON in primary antibody (SOX2, R&D Systems, MAB2018; TBR2, Abcam, ab23345; HOPX, Santa Cruz, sc398703; HOPX, Proteintech, 11419-1-AP; N-cadherin, BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with 2M HCl for 30 minutes and stained with mouse monoclonal anti-BrdU antibody (1:50) (BD Biosciences, San Jose, CA, USA) and Alexa fluor-488 conjugated secondary antibody (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... 5×104 Capan-1 cells and 2.5×104 Hs766T cells were plated in coated BD BioCoat growth factor-reduced matrigel chambers (BD Biosciences, San Jose, CA) for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... after which cells were washed with cold PBS and stained for 1 hr with anti-CD11c antibody for subsequent FACS (BD.LSR Fortessa Cell analyzer).
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bioRxiv - Cell Biology 2022Quote: ... bone marrow cells were isolated and labelled using biotin-conjugated lineage antibody cocktail in combination with PerCP- conjugated streptavidin (BD, 554064; 1:200), and antibodies against SCA-1 ...
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bioRxiv - Systems Biology 2022Quote: ... coli strain JM109 (1 × 1010 colony-forming units (CFU)/ml) were cultured for various times in 2 ml of 2xYT liquid medium (BD Difco, Cat# 244020) containing various concentrations of pyridoxal 5’ -phosphate (pH adjusted to 7.0) ...
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bioRxiv - Microbiology 2019Quote: ... and late stages of fermentation were serially-diluted and plated onto YEPD media (1% (w/w) yeast extract (BD BactoTM, Sparks, MD, USA), 1% (w/w ...
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bioRxiv - Neuroscience 2020Quote: ... anti-O4 (130-118-978) (Milteny Biotec, Bergisch, Germany), anti-O1 (FAB1327V) (Novous Biologicals, Littleton, CO) and anti-CD45 (OX-1 (ROU)) (BD, Franklin Lakes, NJ). Dead cells were excluded using near IR Live/Dead (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... harvested bone marrow cells were labeled using a two-step sequential antibody labeling procedure using the following primary conjugated antibodies: 1:200 dilution of Anti-Mouse CD5 FITC antibody (BD Biosciences, Cat#: 553020), 1:100 dilution of Anti-Mouse B220 FITC antibody (BD Biosciences ...
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bioRxiv - Genomics 2022Quote: ... Cells were labeled with the following antibodies: 1:400 dilution of Anti-mouse CD45 antibody conjugated to APC-Cy7 (BD Biosciences, Cat#: 557659), 1:200 dilution of Anti-mouse TER-119 antibody conjugated to APC-Cy7 (BD Biosciences ...
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bioRxiv - Physiology 2020Quote: ... Sections were blocked in TBS containing donkey serum or PBS/0.1% Triton X-100 and stained for Vimentin (BD pharmingen #550563, 1:2000) and phospo-Akt (CST #4060 1:50 ...
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bioRxiv - Genetics 2020Quote: ... in 100 μl of a 1:200 cocktail of fluorescence-conjugated antibodies to 15 cell surface markers encompassing the major immune lineages B220 (BD, clone RA3-6B2), CD19 (BD ...
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bioRxiv - Immunology 2020Quote: ... The cells were then washed with 1%FBS PBS and stained for 20 minutes at room temperature with the following panel of fluorescently conjugated antibodies diluted in 1%FBS PBS: CD56-PECy7 (BD Biosciences, clone NCAM16.2), CD16-PacBlue (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... Infected cells were selected with 1 μg/ml puromycin and isolated by FACS using a FACS-Aria II cell sorter (Becton Dickinson, BD, Franklin Lakes, USA) based on TurboRFP expression after the induction with 1 µg/ml of Doxycycline
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bioRxiv - Immunology 2020Quote: ... The cells were subsequently washed with FACS buffer (1% BSA and 0.1% sodium azide in phosphate-buffered saline) and stained with anti-HLA-A2 specific monoclonal antibody (clone BB7.2) (BD-Pharmingen, San Diego, CA) at 4°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... washed three times with PBS 1% FBS and permeabilized with 1x Perm/Wash solution (diluted from BD Perm/Wash Buffer 5x in PBS) at RT for 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... cerevisiae cells were grown at 25°C in YPD liquid medium containing 1% (w/v) Bacto yeast extract (BD Biosciences, San Jose, CA), 2% (w/v ...