Labshake search
Citations for Becton, Dickinson and Company :
701 - 750 of 775 citations for Prestained SDS PAGE Standards Single Color since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... and CD45.2+ peripheral blood leukocytes were FACS-sorted and labeled using the BD Rhapsody Single-Cell Multiplexing CTT Kit (BD Biosciences, 626545). On average ...
-
bioRxiv - Immunology 2022Quote: ... Stained cells were resuspended in FACS buffer with 5,000 of 10-µm counting beads (SureCount™ Standards, Bang Laboratories) and analyzed by flow cytometry (LSR Fortessa X20™, BD). Data were analyzed with FlowJo v10 software and cell types identified according to the gate strategy (Fig ...
-
bioRxiv - Immunology 2020Quote: PBMCs were isolated from venous blood samples via standard density gradient centrifugation and used immediately (groups AM, AS, MC, SC, Exp, and BD 2020) or after cryopreservation in liquid nitrogen (group BD 2019) ...
-
bioRxiv - Immunology 2021Quote: ... sequenced reads were aligned to the hg38 human transcriptome and the expression of transcripts in each cell was quantified via the standard Rhapsody analysis pipeline (BD Biosciences) on Seven Bridges (https://www.sevenbridges.com) ...
-
bioRxiv - Microbiology 2021Quote: ... The concentration of antibody was quantified in plates coated in triplicate with two-fold dilutions of an appropriate purified mouse Ig isotype standard (IgG and IgA; BD Biosciences), starting at 0.5 μg/μl ...
-
bioRxiv - Immunology 2021Quote: ... The expression of the standard activation markers CD69 and CD134 (OX40) was quantified on the cell surface using anti-CD69–FITC (clone L78; BD Biosciences) and anti-CD134–BV711 (clone ACT35 ...
-
bioRxiv - Immunology 2022Quote: ... OD values were converted to ng/ml of IgA by comparison with a purified IgA standard (BD Biosciences, San Jose, CA). Fecal extracts from individual mice were obtained as previously described (41 ...
-
bioRxiv - Immunology 2022Quote: ... Anti-Mouse) and propidium iodide as per the standard flow cytometry protocol and analyzed thereafter (LSRFortessa™ X-20 cell analyzer, BD Biosciences ...
-
bioRxiv - Cancer Biology 2023Quote: ... after implementation of the EuroFlow Standard Operating Protocol for Instrument Setup and Compensation in FACSDiva (BD Biosciences, San Jose, CA, USA). Final data analysis was performed in Infinicyt 2.0 (Cytognos ...
-
bioRxiv - Immunology 2023Quote: ... and the 2D effective affinity AcKa was estimated by normalizing
against mr and ml that were measured using PE-labeled monoclonal antibody together with QuantiBRITE PE standard beads (BD Biosciences): -
bioRxiv - Immunology 2023Quote: Single-cell suspensions were analyzed or sorted according to standard protocols using a BD FACS LSRII and BD FACS Aria III (BD Biosciences). Flow cytometry was performed as previously described [52] ...
-
bioRxiv - Neuroscience 2023Quote: ... The adherent cells that remained were either lysed with standard radioimmunoprecipitation assay (RIPA) buffer with 1mM Na3VO4 and BD Baculogold protease inhibitors (BD Biosciences) or 500uL of QIAzol® reagent (Qiagen) ...
-
bioRxiv - Microbiology 2023Quote: ... and values for IL-2 were determined by using a standard curve generated by using known concentrations of purified recombinant murine IL-2 (BD Biosciences).
-
bioRxiv - Biophysics 2024Quote: Human serum was separated from the same whole blood sample along with neutrophil isolation using the standard Vacutainer tubes (Serum Separation Tube, BD, 367989). The serum was aliquoted and stored at a −80 °C freezer (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2024Quote: ... the SRBCs were washed in a standard FACS buffer (1000g for 10 minutes) and stained with anti-mouse IgG-BV605 (BD, 405327) or anti-IgM (BD ...
-
Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... washed 5 times and resuspended in SD-N medium (0.17 % (w/v) yeast nitrogen base without amino acids and ammonium sulfate (BD) and 2 % (w/v ...
-
bioRxiv - Cell Biology 2021Quote: Dissociated hiPSCs were filtered to remove cell aggregates before being clonally isolated using the single-cell deposition function of a BD FACSAria™ III (BD Biosciences). Here ...
-
bioRxiv - Immunology 2020Quote: ... from paired BAL fluid and PBMCs were either bulk sorted in 100-cell mini-populations or single-cell sorted using the FACSAria™ III (BD Biosciences) in purity mode into 96-well microplates containing 10 μL of 1% 2-mercaptoethanol RLT buffer (Qiagen ...
-
bioRxiv - Neuroscience 2021Quote: ... the single cell suspension was added to a plastic petri dish pre-coated with an anti-Thy1 antibody (CD90, BD Biosciences, 550402) and incubated for 10-30 minutes at room temperature ...
-
bioRxiv - Genetics 2022Quote: K562 cells were electroporated using sgRNA guide targeting ASXL1 or SRSF2 genes followed by sorting for live single-cells using BD FACSMelody™ Cell Sorter (BD Biosciences). Sorted K562 cells were plated onto 96-well plates containing 100ul/well RPMI 1640 Medium with L-Glutamine (Biological Industries ...
-
bioRxiv - Immunology 2022Quote: ... Single-cell suspension was obtained by crushing the digested lung tissue through a 100 μm basket filter (BD Biosciences, San Jose, CA) and subsequent lysis of RBCs by resuspending the cell pellet in Ammonium-Chloride-Potassium (ACK ...
-
bioRxiv - Immunology 2022Quote: ... NK cells (NK1.1+NKp46+TRAILnegCD200Rneg) were sorted from pre-gated live/single/CD45+ splenocytes of Rag2-deficient mice using BD FACSAria™ Fusion (BD Biosciences).
-
bioRxiv - Cancer Biology 2023Quote: ... was used to combine and load two samples (tumor tissue and corresponding benign tissue) onto a single BD Rhapsody cartridge (BD Bio-sciences). Sample-tag staining was performed according to the manufacturer’s protocol (sample-tag staining at room temperature for 20 minutes and washing by centrifugation at 400 g for 5 minutes) ...
-
bioRxiv - Neuroscience 2023Quote: ... suspensions were filtered using FACS tube with strainer cap (Falcon 352235) to obtain single cell suspension and live clock neurons were sorted with FACS sorter (BD Aria III). Cells were sorted into 1.5mL DNA low-bind tubes (Eppendorf ...
-
bioRxiv - Bioengineering 2022Quote: Engineered cells were cultured for 3-weeks and flow sorted to dispense single GFP-positive cells into individual wells of 96-well plates in a BD FACSAria™ III (BD Biosciences). NALM6-GFP stable cells from a single colony were expanded ...
-
bioRxiv - Immunology 2023Quote: ... Spleens were harvested and single-cell suspensions were stained with B220 conjugated to fluorescein isothiocyanate (B220-FITC, BD Biosciences, 1:500 dilution), Fas conjugated to PE-cyanine 7 (Fas-PE-Cy7 ...
-
bioRxiv - Cell Biology 2023Quote: ... NIH/3T3 cells were transfected with pSpCas9(BB)-2A-GFP:sgRNA-RhoB and single GFP-positive cells sorted into 96-well plates via FACS using Aria-II SORP (BD Biosciences, Heidelberg, Germany). Cells were expanded and single clones screened for the absence of RhoB expression via immunoblotting ...
-
bioRxiv - Microbiology 2023Quote: ... PI-negative cells were gated and sorted with index sorting (single cell precision mode activated from sorting layout in the BD FACSDiva software) onto Mueller Hinton agar (MHA ...
-
bioRxiv - Microbiology 2024Quote: ... Stained memory B cells (MBCs; CD3/CD14/CD56- CD19+ CD27+ IgD- IgM-) were single-cell sorted with a BD FACSAria™ Fusion (BD Biosciences) into 384-well microplates containing 3T3-CD40L feeder cells and were incubated with IL-2 and IL-21 for 14 days71.
-
bioRxiv - Cell Biology 2023Quote: ... and D24 were enzymatically dissociated to single cell suspension and stained with BD Horizon™ Fixable Viability Stain 510 (FVS510, BD Biosciences). Live single cells were sorted onto 384-wells containing primers with unique molecular identifiers (UMIs) ...
-
bioRxiv - Cell Biology 2024Quote: For single-cell RNA sequencing experiments cells were washed and stained with anti-CD45-PE/Cy7 (BD Pharmingen, clone 30-F11, #552848) and SYTOX™ Green Ready Flow™ Reagent (Invitrogen ...
-
bioRxiv - Immunology 2024Quote: ... Single-cell suspensions were stained with antibodies listed in Table S1 and acquired with a LSR Fortessa flow cytometer (BD Bioscience, US). For cell-associated autofluorescence to be removed in the indicated experiments ...
-
bioRxiv - Immunology 2022Quote: ... and the supernatants were harvested for cytokine detection (IL-10 and TGF-β) by specific ELISAs using a standard protocol (BD OptEIA). The detection limits for IL-10 is 31.3–2000 pg/ml e TGF- β is 62.5–4,000 pg/mL) ...
-
bioRxiv - Neuroscience 2022Quote: ... defined as the ratio (expressed in %) of the average channels standard deviation to the metric mean value (either IBI, BD or IBSR). The lower the CV ...
-
bioRxiv - Neuroscience 2022Quote: ... defined as the ratio (expressed in %) of the average channel standard deviation to the metric mean value (either IBI, BD or IBSR). The lower the CV ...
-
bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was done by standard techniques following 5-hour stimulation with Gp61-80 peptide and Brefeldin A (GolgiPlug™, BD Biosciences). No peptide controls were treated under the same conditions supplemented with Brefeldin A but without Gp61-80 peptide ...
-
bioRxiv - Cell Biology 2022Quote: ... For nitrogen starvation in SD-N medium (0.17% (w/v) yeast nitrogen base without amino acids and ammonium sulfate (BD Difco) and 2% (w/v ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were grown in SDC overnight to early log-phase and then washed at least three times in synthetic defined without nitrogen (SD-N) media composed of 0.17% (w/v) yeast nitrogen base without amino acids and ammonium sulfate (BD Difco) and 2% (w/v) ...
-
bioRxiv - Cancer Biology 2021Quote: The single-cell suspension of each tissue was obtained by passing the cell suspension through a 35 μm cell strainer (BD Biosciences, CA, USA). After dissociating of the organs into a single-cell suspension ...
-
bioRxiv - Microbiology 2021Quote: ... were plated on rich Sabouraud agar plates with single colonies subsequently picked and maintained in Difco™ Sabouraud Dextrose Broth (BD, Sparks, MD) at 30° C with agitation for 18 hr ...
-
bioRxiv - Microbiology 2021Quote: Analysis of fluorescent reporter outputs at the single cell level was performed using the BD Accuri™ C6 Plus flow cytometer (BD Bioscience, Germany). The chromophore of the yellow fluorescent protein eYFP or Venus was excited with a blue dye laser with an excitation wavelength of 488 nm ...
-
bioRxiv - Cancer Biology 2020Quote: ... OVN62 cells heterogeneously expressing inducible-Cas9 were performed single-cell sorting using a FACS Aria Ⅲ Cell Sorter (BD Bioscience, San Jose, CA) to pick up stably expressing inducible-Cas9 construct.
-
bioRxiv - Immunology 2022Quote: Surface staining: Single cell suspensions were incubated with 5% normal rat serum and rat anti-mouse CD16/CD32 antibody (BD Biosciences close 2.4G2) then stained with fluorophore-conjugated antibodies ...
-
bioRxiv - Developmental Biology 2022Quote: ... cells were dispersed into a single cell suspension using 0.05 % trypsin and subsequently incubated with an anti CD144 antibody (anti-VE-Cadherin; BD Biosciences, #562242, 1/50) for 30 min on ice ...
-
bioRxiv - Cell Biology 2023Quote: ... Immunostaining of the single-cell population was performed using primary antibodies followed by phycoerythrin (PE)-conjugated secondary antibodies (BD Biosciences, San Jose, CA) for fluorescent labeling ...
-
bioRxiv - Immunology 2023Quote: ... Note these experiments were carried out using early access kits from BD Genomics before the implementation of commercially-available single-cell protein/RNA assays (e.g. Feature Barcoding, 10x Genomics; BD Abseq, BD Genomics, Supplemental Table 4), and researchers are recommended to use those newer solutions for any follow-up studies as the techniques and reagents have been refined ...
-
bioRxiv - Neuroscience 2019Quote: ... the slices were transferred to the standard external solution in a 35-mm culture dish (BD Falcon 353001, BD, Franklin Lakes, NJ, USA) coated with silicone ...
-
bioRxiv - Microbiology 2021Quote: ... A 1.5-fold testing scale was used to include the standard two-fold antibiotic concentrations and their median values and the cation-adjusted Mueller-Hinton broth (CAMHB - BD, New Jersey, USA) was supplemented with 100 U of catalase (Worthington Biochemical Corporation ...
-
bioRxiv - Genomics 2022Quote: ... Standard protocol check to ensure instrument repeatability was done using analysis of Spherotech 8-Peak Validation Beads (BD Biosciences, San Jose, CA, #653144). Flow cytometry data was analyzed using BD CSampler Plus Analysis software to take the mean fluorescence of each peak of the fluorescence versus count histogram ...
-
bioRxiv - Immunology 2023Quote: ... Fixed tissues were then embedded into wax for histological purposes before being cut and stained using a standard H&E protocol, the previously described method for GSDMD staining (Robinson et al., 2020) plectin (BD Transduction Labs, #611348) or plakoglobin (Progen #61005S ...