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Citations for Becton, Dickinson and Company :
7201 - 7250 of 7387 citations for 7 Methylimidazo 1 2 a pyridine 6 boronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Spleens were harvested and single-cell suspensions were stained with B220 conjugated to fluorescein isothiocyanate (B220-FITC, BD Biosciences, 1:500 dilution), Fas conjugated to PE-cyanine 7 (Fas-PE-Cy7 ...
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bioRxiv - Genomics 2023Quote: Trypsinized or MACS sorted cells were incubated at 4°C for 20 min with APC-conjugated anti-CD56 antibodies (1:40, BD Pharmingen #555518) or isotype control (1:40 ...
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bioRxiv - Immunology 2022Quote: ... with the following antibodies/reagents (all at 1:200 dilution) for 30 min on ice: anti-CD20-PE-Cy7 (BD Biosciences, 335828), anti-CD14-APC-eFluor 780 (Thermo Fischer Scientific ...
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bioRxiv - Genetics 2023Quote: Fluorescently conjugated antibodies for cell surface staining (Supplementary Table 1) were prepared in Brilliant Stain Buffer (Cat. no. 556349, BD Biosciences, Australia) and incubated with cells in a 96 well plate (Cat ...
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bioRxiv - Immunology 2024Quote: ... samples were washed with 1X PBS and fixed in 1% formaldehyde before acquisition on a FACSCanto™ II (Beckmann Coulter) using the FACS DiVa software (BD, Biosciences). Also ...
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bioRxiv - Immunology 2024Quote: ... Stained samples were fixed in 1% formaldehyde and acquired on FACSCanto™ II (Beckmann Coulter) using the FACS DiVa software (BD, Biosciences). Data were analysed using FlowJo v10.6 (Tree Star Inc) ...
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bioRxiv - Bioengineering 2024Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: We performed extracellular staining using the following antibodies (dilution, clone, company, catalog number): anti-mouse CD45.2 BUV395 (1:100, 104, BD Biosciences, Cat. 564616), anti-mouse CD3 BUV737 (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... the stromal vascular fraction was sorted into 96-well plate at 1 cell per well using BD FACSAria II Cell Sorter (BD Biosciences, USA). The cells were spontaneously immortalized by passaging for more than 30 passages ...
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bioRxiv - Immunology 2024Quote: ... Samples were then spun down at 300 × g for 5 minutes at 4°C and resuspended in full antibody cocktail containing fluorophore-conjugated antibodies (Table 1) and FC block in BD Horizon Brilliant Stain Buffer (BD Biosciences, #563794). Cells were incubated with antibodies for 20 minutes at 4°C before three washes in FACS buffer containing 0.25mM EDTA ...
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bioRxiv - Immunology 2024Quote: ... Samples were washed 3x after staining and resuspended in 1% BSA for analysis on an a for analysis on an LSRFortessa flow cytometer (BD Life Sciences).
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bioRxiv - Bioengineering 2024Quote: The expression of the exhaustion markers PD-1 and TIM-3 was evaluated using anti-PD1 (cat. 560795, BD Biosciences, BD PharMingen) and anti-TIM3 (cat ...
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bioRxiv - Systems Biology 2024Quote: ... and lawns of pAWP78-P89-20ZRfae in E.coli S17-1 (overnight) were prepared for mating on plates with Hypho media supplemented with 5% Nutrient Broth (NB Difco, BD Life Sciences). Plates were incubated for 1 day at 30° C ...
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bioRxiv - Immunology 2024Quote: ... A cocktail of the surface stain (Supplemental Table 1) supplemented with BD Horizon™ Brilliant Stain buffer (BD Biosciences, Cat no. 566349) was added to each well (50 μL/well) ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed/permed with Foxp3 Transcription Factor Fixation/permeabilisation (eBiosciences, RELMα, iNOS, Ym-1) or IC Fixation/permeabilisation buffer (BD, intracellular cytokines) with paired perm/wash buffers ...
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bioRxiv - Immunology 2024Quote: ... The interphase was then re-suspended in FACS buffer and blocked with the Fc-blocker CD16/CD32 (553141, BD Biosciences, 1:100) for 20 minutes at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated with anti-mouse CD32 for 5 min at 4°C to block Fc receptors and stained with fluorochrome conjugated antibodies to CD45R/B220 PE (1:200) (clone RA3-6B2, BD Bioscience #553089), Ter119 PE (1:200 ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were then incubated for 1 h with primary antibodies directed against the cis-Golgi marker GM130 (mouse anti-GM130, BD bioscience, 610822) and/or trans-Golgi marker TGN46 (sheep anti-TGN46 ...
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bioRxiv - Microbiology 2024Quote: ... The urine samples were obtained in sterile BD Vacutainer® urine C&S Preservative Tubes (BD, 1 Becton Drive, Franklin Lakes. NJ). Specifically ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked with 5% skimmed milk in TBS-Tween and incubated with anti-αSyn clone 42 (BD Biosciences, 1:1,000 dilution). In a slot blot ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 1 ml of whole blood was collected from each mouse by retro-orbital extraction into lithium-heparin tubes (BD Medical, #368496) containing 1.5 ml pre-warmed culture medium lacking serum ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed with FACS staining buffer and incubated with α-BrdU antibody conjugated to APC (1:50, BD Pharmigen flow kit) and α-pHH3 (Ser10 ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed twice with 1% FBS in PBS (staining buffer) before incubation with 2.5 μg Fc block (BD Biosciences Cat 564219) in staining buffer for 15 min at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... 1:200 diluted human Actinin(Sarcomeric)-PE (Miltenyi, 130-123-996) and 1:400 diluted mouse Aurora B primary antibody (BD Bioscience, 611082) were added at 4°C in the dark for 16–24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Mice were injected 1 day post arthritis induction using 0.5 ml monoject (29G) insulin syringes (BD Micro-Fine, Franklyn Lakes, NJ, USA) through the patellar ligament into the knee joint ...
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bioRxiv - Cancer Biology 2024Quote: Four to six-week-old female BALB/c nude mice (T cell deficient) were subcutaneously injected with 1 × 107 SNU-16 cells suspended in 100 μL PBS with 50% Matrigel (BD Biocoat, 356234). When the tumor volume reached approximately 100-150 mm3 ...
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bioRxiv - Cancer Biology 2024Quote: ... To probe IdU, the samples were incubated with mouse anti-IdU antibody (Abcam, ab6326; 1:25 in blocking buffer (BD Bioscience, 347580)) for 60-minutes each at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: Single cell suspensions of lymphocytes from tumors were pre-treated with anti-CD16/CD32 Mouse Fc block (BD Biosciences 553142, 1:100) following by staining with fluorescently labelled antibodies against ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 ml of cell suspension was incubated with 2 μl of Nile red solution (0.25 mg ml−1) for 5 minutes before the analysis with a bench flow cytometer (BD Accuri C6, BD Biosciences). To determine Nile red fluorescence ...
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bioRxiv - Cancer Biology 2021Quote: ... PE mouse anti-human VCAM-1 or Alexa Fluor 647 mouse anti-human CD31 (all antibodies were from BD, Franklin Lakes, NJ, USA). Samples were analyzed on Gallios Flow Cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... The antibodies used for the flow cytometry analysis of the cell surface markers were: anti-human CD11b (740965, BD OptiBuild™, 1:100), anti-human CD14 (11-0149-42 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with a fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD31 antibody (1:50; BD Pharmingen, San Diego, CA, USA) for 30 min at room temperature followed by 3 washes with phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... Microglia were washed 3x with PBS before incubating them in iTF-Microglia media supplemented with 1:2000 GolgiPlug™ (BD; Cat. No. 555029) for 4h ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated for 60 minutes in 1% BSA and 5% goat serum in PBS and then incubated overnight with anti-p21 antibody (BD Pharmingen, mouse, #556431) and anti-p16 antibody (abbiotec ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then left at 4°C overnight in a blocking solution containing the primary antibody: mouse E-Cadherin (BD Biosciences, 1:1000) and mouse N-Cadherin (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... the cryosections were incubated for 1 h in the blocking solution (PBS containing 5% goat serum and 5 μg/mL mouse BD Fc Block™) and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... 100 μl of the bacterial inoculum was transferred from a Hungate tube to under oil in the eppendorf tube using a syringe (1 ml, Luer-lok Tip, BD Biosciences, REF 309628). Bacteria were added to the media above the colon monolayer by pipette at 1:20 v/v ratio (2 μl bacteria:40 μl media ...
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bioRxiv - Cell Biology 2021Quote: ... cells were rinsed twice in sort medium before being stained with Propidium Iodide (PI, 1:100) to stain dead cells and sorted with a FACSAria III flow cytometer (BD Biosciences, Heidelberg, Germany) as previously described (Cesetti et al. ...
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bioRxiv - Microbiology 2021Quote: ... were anesthetized and a sterile 1-cm long catheter segment (Qosina) inserted into the peritoneum using an 18-gauge BD Spinal needle/stylette (Becton, Dickinson and Company,). Three hours post-catheter implantation ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for ON in primary antibody (SOX2, R&D Systems, MAB2018; TBR2, Abcam, ab23345; HOPX, Santa Cruz, sc398703; HOPX, Proteintech, 11419-1-AP; N-cadherin, BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with 2M HCl for 30 minutes and stained with mouse monoclonal anti-BrdU antibody (1:50) (BD Biosciences, San Jose, CA, USA) and Alexa fluor-488 conjugated secondary antibody (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... 5×104 Capan-1 cells and 2.5×104 Hs766T cells were plated in coated BD BioCoat growth factor-reduced matrigel chambers (BD Biosciences, San Jose, CA) for 24 hours ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were incubated overnight at 4 °C with primary mouse monoclonal anti-alpha synuclein antibody (Cat no. 610787; BD Biosciences; 1:200) and for 2 hr with secondary goat anti-mouse Alexa Fluor Plus 488 antibody (Cat no ...
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bioRxiv - Neuroscience 2020Quote: ... astrocytes were first starved (DMEM without serum) for one hour and subsequently incubated with the 9EG7 antibody (BD Bioscience, 1:20 in DMEM) on ice for 1 h ...
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bioRxiv - Immunology 2022Quote: ... after which cells were washed with cold PBS and stained for 1 hr with anti-CD11c antibody for subsequent FACS (BD.LSR Fortessa Cell analyzer).
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bioRxiv - Cell Biology 2022Quote: ... bone marrow cells were isolated and labelled using biotin-conjugated lineage antibody cocktail in combination with PerCP- conjugated streptavidin (BD, 554064; 1:200), and antibodies against SCA-1 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1.5 mL of each milt sample was diluted 1:9 in Storfish (IMV Technologies, France; an inactivating medium) in 15 mL tubes (Falcon, BD Biosciences, Allschwil, Switzerland). These tubes were then placed on ice and are referred to as ‘diluted milt’.
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated in the FC wash buffer (2h at 4 °C) containing FITC-coupled anti-CD44 antibody (1 to 80 BD Pharmingen Cat.No. 555478) or its isotype control (FITC Mouse IgG2b ...
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bioRxiv - Neuroscience 2020Quote: Brains from paraformaldehyde-perfused 12 month-old mice were sectioned and 30 µm free-floating sections were incubated overnight at 4°C with primary antibodies (anti-αsyn, BD Transduction, 1:700), anti-DNAJC5 (Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... anti-O4 (130-118-978) (Milteny Biotec, Bergisch, Germany), anti-O1 (FAB1327V) (Novous Biologicals, Littleton, CO) and anti-CD45 (OX-1 (ROU)) (BD, Franklin Lakes, NJ). Dead cells were excluded using near IR Live/Dead (Thermo Fisher Scientific ...