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Citations for Becton, Dickinson and Company :
7101 - 7150 of 7345 citations for 7 Methylimidazo 1 2 a pyridine 6 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS and resuspended in 0.5 ml of PBS containing 1 μg/ml propidium iodide (PI) and 50 μg RNase and analyzed by flow cytometry (BD LSR-II) MCF-7 cells were exposed to 10 Gy γ radiation ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pelleted and washed twice with 1% FBS in PBS and subjected to flow cytometry using a FACSMelody™ (BD Biosciences).
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bioRxiv - Immunology 2021Quote: Mouse splenocytes were cultured in RPMI-10 and stimulated with the S peptide pool at 1 µg/ml/peptide in the presence of GolgiStop™ and GolgiPlug™ (BD) for 6 hours ...
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... was added after 1 hour and cells were stained for surface NK makers (CD56 and CD16) and intracellular IFN-γ (Cytofix/Cytoperm; BD Biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... 0.1% sodium azide) and re-suspended in 500µl of ice-cold FACS buffer prior to flow cytometric analysis (BD LSR II Fortessa). Data were analyzed using the FCS Express software.
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bioRxiv - Cancer Biology 2020Quote: ... at a density of 1 million cells per 100 μL and stained with anti-mouse CD16/CD32 Fc Block™ (BD Pharmigen) for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... Sections were washed and stained with primary antibody to E-cadherin (1:100 anti E-cadherin mouse monoclonal antibody, BD Biosciences 610181) and/or primary antibody to O157-antigen (1:2000 anti E ...
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bioRxiv - Microbiology 2021Quote: ... 2×106 lymph node cells were stained with the following monoclonal antibodies: rat anti-mouse CXCR5-biotin (clone 2G8, 1:100, BD, cat#551960), streptavidin PECy7 ...
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bioRxiv - Cell Biology 2022Quote: ... washed three times with RPMI and incubated for 30 mins at 37°C in RPMI containing Dispase (BD Cornig, 1:10 dilution) and 30 μg/μl DNase I with continuous and gentle agitation on a rotating shaker ...
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bioRxiv - Neuroscience 2022Quote: ... The primary and secondary antibodies and dilutions used here were: mouse anti-BrdU 1:100 (347580; BD Biosciences, Franklin Lakes, NJ, USA), 4c4 1:200 (mouse anti-fish leukocytes ...
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bioRxiv - Microbiology 2020Quote: The mitochondrial inner membrane potential (Δψm) was determined by staining with the membrane-permeable lipophilic cationic fluorochrome JC-1 (BD Biosciences, NJ). Overnight C ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA extraction and BrdU-labelled DNA immunoprecipitation were performed with anti-BrdU antibody (40 μL of 25 μg mL−1, BD Pharmingen, #555627). Validation of BrdU immunoprecipitation was carried out using qRT-PCR on known Early (BMP1 ...
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bioRxiv - Immunology 2020Quote: ... and serum-blocked in 2.5% goat serum/PBS containing 1:100 dilution of Fc block (CAT # 553142; BD Biosciences, San Jose CA). For B ...
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bioRxiv - Microbiology 2020Quote: ... Each culture contained a 10 μL 4.0 McFarland (McF) bacterial cell suspension (WHO-F or WHO-X) in 12 mL gonococcal (GC) broth supplemented with 1% IsoVitaleX (BD BBL™) and vancomycin ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in blocking solution of 1% normal horse serum in PBS with 0.3% Triton X-100 and incubated overnight at 37°C as follows: mouse (IgG1) anti-CtBP2 (C-terminal Binding Protein) 1:200 (#612044, BD Transduction Labs) targeting pre-synaptic ribbons ...
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bioRxiv - Microbiology 2021Quote: ... the same protocol was followed except that of the use of biotinylated anti-Syrian hamster IgG1 /IgG2 antibodies in 1:10000 dilution (BD biosciences, USA) and Streptavidin-horseradish peroxidase 1:8000 (Thermo-scientific ...
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Mutation in the matricellular gene fibulin-4 leads to endothelial dysfunction in resistance arteriesbioRxiv - Physiology 2022Quote: ... Membranes were blocked in 5% BSA/0.1% Tween20 in 1x TBS or 5% milk/0.1% Tween20 in 1x PBS for primary antibody mouse anti-human phospho-eNOS (1:1,000, pS1177, #612392, BD Biosciences, Franklin Lakes, NJ) or mouse anti-human-eNOS (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed the pellet was resuspended with 1 ml PBS and analyzed in a Flow Cytometer LSRFortessa (BD Biosciences, NJ, USA). Analysis of the data output was preformed via FlowJo v10.8 software using Watson model to calculate the percent of cells in each stage of the cell cycle.
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bioRxiv - Cell Biology 2022Quote: ... Hematopoietic cells were harvested from OP9-DL1 cocultures by forceful pipetting and stained on ice for one hour with the following antibodies: Pacific Blue CD4 (1:500; BD Pharmingen, 558107), BV711 CD8a (1:1000 ...
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bioRxiv - Bioengineering 2022Quote: ... HAECs were allowed to recover from passaging for 24 h after plating and were subsequently incubated with AlexaFluor-647 anti-human VE-Cadherin antibody (1:100, BD Biosciences, #561567). After 15 minutes incubation ...
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bioRxiv - Developmental Biology 2019Quote: ... Then the cells were washed twice in 1 ml PBS with 10% FBS and examined on a BD FACSAria™ III cell sorter (BD Biosciences).
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bioRxiv - Molecular Biology 2019Quote: Whole-mount retinas were blocked with horse serum 4°C overnight then incubated at 4°C for 3 days with primary antibodies: Mouse anti-Oct4 (1:100, BD bioscience, 611203), Rabbit anti-Sox2 (1:100 ...
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bioRxiv - Plant Biology 2019Quote: ... 9-cm Petri plates on modified Hoagland medium (Baskin and Wilson, 1997) containing 1% (w/v) Sucrose and 0.8% (w/v) agar (Difco Bacto agar; BD Laboratories, http://www.bd.com). Two days after stratification at 4°C in the dark ...
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bioRxiv - Microbiology 2019Quote: ... The resulting solutions were diluted (10-1 - 10-4) and 10 µl were seeded in plates containing potato dextrose agar (PDA) (BD-Difco®) and Sabouraud agar (SBA ...
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bioRxiv - Bioengineering 2019Quote: ... Isolated splenocytes were plated onto adherent culture dishes and activated with anti-mouse antibodies against CD3ε(1:1000 dilution, BD Biosciences 553058) and CD28 (1:1000 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were re-blocked in PBSB for 15 minutes and incubated with anti-BrdU antibody (BD-Pharmingen 555627, 1:200 in PBSB) overnight at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... double emulsions were diluted 1:5 in FACS diluent buffer in a 12 × 75 mm round bottom FACS tube (BD Bio-sciences). For a typical run ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were then blocked with 5% non-fat milk and probed with the following antibodies: β-catenin (1: 3,000, BD Biosciences, USA), Wnt6 (1 ...
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bioRxiv - Cell Biology 2021Quote: ... were fixed on ice with a solution of 1% paraformaldehyde and incubated with fluorescent antibody to platelet markers CD41a (BD Biosciences #555467), activated integrin αIIbβ3 (BD Biosciences #340507) ...
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 1 h with primary antibodies to RAD51 (B-bridge International, #70-001) and to cyclin A (BD Biosciences, # 611268) diluted in 1% BSA ...
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bioRxiv - Genetics 2020Quote: ... the harvested hNC cells were subjected to fluorescence-activated cell sorting (FACS) and hNC cells which were positive to both HNK-1 (BD Biosciences #560845) and p75NTR (Miltenyi Biotec #130-091-917 ...
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bioRxiv - Cancer Biology 2019Quote: ... NK1.1) and later stained for intracellular molecules (IFN-γ; Foxp3) following fixation and permeabilization according to the manufacturer’s protocols (BD Cytofix/Cytoperm). Data were collected on the LSRII- Fortessa (BD ...
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bioRxiv - Genetics 2020Quote: ... 0.5 × 106 DAPI-/SSEA4+/TRA-1-60+ cells were collected for each cell line using the BD FACSAria™ II flow cytometer (BD Biosciences). Collected cells were pooled and plated at a density of 30 000 /cm2.
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bioRxiv - Evolutionary Biology 2020Quote: ... reduced to a single-cell suspension and seeded into a 96-well plate at a density ranging between 1000 – 20 c/w in mTesRTM1 supplemented with 1 nM ROCK inhibitor (BD Biosciences, 562822). Alternatively ...
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bioRxiv - Cell Biology 2020Quote: ... The following antiboodies were used in combination for the depletion of lineage-positive cells: anti-CD4 (RM4-5, BD Biosciences, 1:100), -CD8 (53-6.72 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were allowed to dry and then hydrated and blocked for 1h at RT with 5%milk/TBS-T and incubated overnight at 4ºC with the primary antibody mouse monoclonal anti-α-Syn 1:1000 (BD Biosciences, 610787) in 5%milk/TBS-T ...
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bioRxiv - Developmental Biology 2021Quote: ... for 60 min, the sections were reacted with the primary antibody (for CD31, 1:100 rat monoclonal anti-CD31 (BD Biosciences, 550274); for Cytokeratin ...
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bioRxiv - Cell Biology 2021Quote: Cells were treated with 25uM BrdU for 1 hour and BrdU staining detected using FITC Mouse Anti-BrdU Set (BD Biosciences, 556028). Cells were resuspended in 10μg/ml 7-Amino-Actinomycin D (7-AAD ...
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bioRxiv - Cell Biology 2021Quote: ... The ZsGreen-ODC (ornithine decarboxylase) cells were previously generated by transfection of MelJuSo cells with the ZsProsensor-1 plasmid (BD Bioscience Clontech)20 ...
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bioRxiv - Microbiology 2020Quote: RNA and virus injections of 5 and 10 μl were performed with insulin syringes (BD Micro Fine Plus, 1 ml, 30 G) into honey bee and bumble bee pupae respectively ...
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bioRxiv - Immunology 2021Quote: ... For cell staining cells were first blocked with 1 μg/ml Fc-Block (mouse Fc Block, BD Biosciences, San Jose, CA, USA) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... enriched tumor-infiltrating CD8+ T cells were re-stimulated with 1 μg/ml ionomycin (IM) and 25 ng/ml phorbol myristate acetate (PMA) in the presence of GolgiPlug (BD Biosciences, USA) for 4 hours at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... up to 5×106 leukocytes were incubated with 5 μg/mL E6 peptide or 1 μg/mL α-CD3e (clone 145-2C11, BD Biosciences) in the presence of 1:1000 Golgi-plug for 5 hours ...
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bioRxiv - Immunology 2020Quote: ... and BV421 rat anti-mouse TNF-α BD Biosciences in a total of 100 μL of 1× Perm/Wash Buffer (BD Biosciences). After two washes ...
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bioRxiv - Immunology 2020Quote: Cryopreserved lymphocytes from BAL were thawed and stimulated with H1N1pdm09 (MOI 1) or medium as a control for 18 hours at 37°C prior GolgiPlug (BD Biosciences, UK) addition as per manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... The cells were blocked with 4% BSA and stained with a 1:200 (v/v) dilution of Alexa Fluor 647 Mouse anti CD247 pY142 antibody (BD Bioscience #558489) for 1 hour at room temperature ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... After washing with 1% BSA-PBS slides were incubated with secondary goat anti-rat and goat anti-rabbit biotinylated antibodies for 30 min at RT followed by 30 min of incubation with streptavidin–HRP (1:500; BD Pharmingen, 554066). For developing DAB substrate (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... before resuspending in 25 μl of 0.2µm-filtered 1% BSA/PBS containing monoclonal FITC-anti-mouse IgA (BD Pharmingen, 10 µg/ml) or Brilliant violet 421-anti-IgA (BD Pharmingen ...