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Citations for Becton, Dickinson and Company :
651 - 700 of 703 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... splenocytes were first surface stained for GC markers then washed twice in staining buffer and treated with fixation/permeabilization solution Cytofix/Cytoperm (BD Bioscience, 554722) for 30 minutes on ice in the dark ...
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bioRxiv - Neuroscience 2021Quote: ... the connection between the cement and the scalp was disinfected by iodine solution and cannulae were blocked by insertion of stainless steel stylets (25 G, BD, Heidelberg, Germany) to prevent dust entering the cannulae that could result in infection or blockage ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed 1 time with 100 µL PBS and fixed and permeabilized in 100 µL Fixation and Permeabilizaton Solution from the BD Cytofix/Cytoperm Fixation/Permeabilization Kit (BD BioSciences 554714) for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 200 µL of PI (from 50 µg/ml stock solution) was then added and the cell cycle was analyzed by flow cytometry (BD FACSCanto™ II, BD Bioscience) using untreated cells as negative control for gating ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed 1 time with 100 µL PBS and then fixed and permeabilized in 100 µL Fixation and Permeabilization Solution from the BD Cytofix/Cytoperm Fixation/Permeabilization Kit (BD BioSciences, 554714) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII flow cytometer (BD Bioscience, San Jose, CA).
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA).
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bioRxiv - Biochemistry 2020Quote: ... and CD81 antibodies (with no beads) were added to the solution containing the EVs (PE Mouse Anti-Human CD81 Clone JS-81, BD Pharmingen™). After 1 h of incubation ...
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bioRxiv - Cell Biology 2021Quote: ... were fixed on ice with a solution of 1% paraformaldehyde and incubated with fluorescent antibody to platelet markers CD41a (BD Biosciences #555467), activated integrin αIIbβ3 (BD Biosciences #340507) ...
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bioRxiv - Genetics 2020Quote: ... 10 × 106 cells from villages of iPSCs or neurons were fixed and permeabilized using the Fixation/Permeabilization Solution Kit (BD Biosciences, 554714). Cells were resuspended in FACS buffer (1 x PBS ...
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bioRxiv - Immunology 2023Quote: ... Stimulated cells were washed once in FACS buffer before fixation for 20 min at 4°C using the BD Cytofix/Cytoperm solution and permeabilization using BD Perm/Wash™ buffer (BD Biosciences). Permeabilized cells were incubated for 30 min with antibodies (diluted 1:200 in BD Perm/Wash ...
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bioRxiv - Neuroscience 2022Quote: ... The skin was prepared with Povidone-Iodine solution (Aplicare, INC., Neriden, CT USA) followed by 70% alcohol swab (BD, Franklin Lakes, USA). After Lidocaine (0.2 ml ...
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bioRxiv - Cell Biology 2023Quote: ... and the solution was mixed and placed on ice and analyzed with a FACSCalibo flow cytometer (BD Bioscience, Franklin Lake, NJ, USA) within one hour ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... Permeabilization was followed by blocking cells using 5% BSA in HBSS+ for 30 min and incubated with the primary antibody solution - Mouse anti-human CD144 (1:150; 555661, BD Biosciences, USA) for overnight at 4°C ...
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bioRxiv - Pathology 2022Quote: ... The resulting suspension of muscle fibres was then disrupted by passing it through an 18-gauge needle 10 times before passing the resulting solution through a 40 μm cell strainer (BD Falcon 352340).
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bioRxiv - Bioengineering 2022Quote: ... the membrane was incubated overnight at 4°C or with primary antibody solution (1:1000 dilution for anti-Alix [E4T7U, Cell Signaling Technology], anti-QSOX1 [12713-1-AP, ProteinTech], anti-HIF-1α [610958, BD Biosciences] ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with 2% FACS buffer with 50nM dasatinib and fixed using fixation/permeabilization solution (Cytofix/Cytoperm™ fixation and permeabilization kit, BD Biosciences) for 20 minutes at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 μL of PBS containing 5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... After washing the cells with blood FACS buffer we performed red blood cell lysis and fixation using BD FACS™ Lysing Solution 10X Concentrate (BD, 349202) diluted 1:10 in ddH2O ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 ml of cell suspension was incubated with 2 μl of Nile red solution (0.25 mg ml−1) for 5 minutes before the analysis with a bench flow cytometer (BD Accuri C6, BD Biosciences). To determine Nile red fluorescence ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then left at 4°C overnight in a blocking solution containing the primary antibody: mouse E-Cadherin (BD Biosciences, 1:1000) and mouse N-Cadherin (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... the cryosections were incubated for 1 h in the blocking solution (PBS containing 5% goat serum and 5 μg/mL mouse BD Fc Block™) and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were stained with 10 μg/ml propidium iodide (PI) staining solution from FITC Annexin V Apoptosis Detection Kit (BD Pharmingen; Cat#: 556547) and 0.2 mg/mL RNase A (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: 3 × 106 nuclei in nuclei isolation buffer were centrifuged at 700 g for 10 minutes fixed by adding 100 µl BD Cytofix/Cytoperm solution (BD Biosciences, Cat# 554714) while vortexing and incubated for 15 minutes on ice ...
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bioRxiv - Plant Biology 2020Quote: The protoplast-filled digestion solution was first filtered through a Falcon 100 μm nylon cell strainer (352360; BD Biosciences, San Jose, CA, USA) in a sterile petri dish and then through a Falcon 40 μm nylon cell strainer (352340 ...
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bioRxiv - Cancer Biology 2022Quote: ... Live cells were selected based on their non-permeability and lack of fluorescence associated with DAPI Solution (BD Biosciences, Franklin Lakes, NJ, USA) or 7-AAD Viability Staining Solution (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... washed three times with PBS 1% FBS and permeabilized with 1x Perm/Wash solution (diluted from BD Perm/Wash Buffer 5x in PBS) at RT for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... IdU and CldU were then detected with the following primary antibodies in blocking solution for 1 hour at room temperature: mouse anti-BrdU (B44) (1:25) (BD Biosciences cat#347580) for IdU and rat anti-BrdU [Bu1/75 (ICR1)] (1:25 ...
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bioRxiv - Physiology 2021Quote: ... Following resuspension in PBS and passage through a 70μm strainer, cells were incubated in Fc block solution (Bio Rad, Cat# BUF041A) and PerCP-conjugated anti-CD11b antibody (BD Pharminogen, Cat# 550993). After PBS washes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed with Hank’s buffered saline solution and resuspended in 2mM EDTA/ 2% BSA/ PBS buffer containing [2µg/mL] propidium iodide (PI) (BD Biosciences, Cat. No. 556364) and [1µM] Calcein violet (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Intracellular staining was performed after fixation and permeabilisation of the cells using FOXP3 Fixation/Permeabilisation Solution kit (BD Biosciences, Frankin Lakes, NJ, USA) using manufacturer’s instruction ...
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bioRxiv - Genomics 2022Quote: ... 1ml diluted blood was transferred into a clean 15ml-tube and incubated with 9ml 1x red blood cells lysing solution (BD Pharm Lyse, 555899) at room temperature for 15min avoiding light ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667, 1:100 in 1X BD Perm/wash solution) or anti-BST2 (Proteintech #13560-1-AP ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mL of 0.1% FBS/PBS was added to the cell suspension and the solution was passed through a BD Falcon 70μm strainer (BD Biosciences, San Jose, CA) to remove any undigested tissue ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed with PBS and staining solution before they were fixed and permeabilized according to the manufacturer’s instructions (BD Biosciences, San Diego, CA). Cells were subsequently stained with anti-BrdU antibody solution at room temperature for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated overnight at 4°C in solution containing 1% BSA and primary antibodies: mouse F4/80 (T45-2342, BD Biosciences, New Jersey, USA) 1:200 ...
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bioRxiv - Molecular Biology 2020Quote: ... The base of the UC of the CIM plate was coated with 20% solution of a basement membrane matrix (BD Matrigel, BD Biosciences, San Jose, CA) in serum-free medium ...
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bioRxiv - Immunology 2021Quote: ... surface stained, fixed with 4% formaldehyde solution (Tousimis, Rockville, MD) and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA). Antibodies used for flow cytometry included anti-human CD3 AF700 (clone UCHT1) ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville MD) and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean of the fluorescence intensity of bead-positive cells and dividing by 10,000.
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bioRxiv - Immunology 2020Quote: ... cells were fixed and permeabilized for 30 min at 4 °C in the dark after extracelluar stainings using BD Cytofix/Cytoperm (Fixation and Permeabilization Solution, BD Biosciences, Cat: 51-2090KZ) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for 30 min at room temperature in the staining solution before being subjected to flow cytometry analysis (FACSCalibur, BD Biosciences, San Jose, CA).
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... A non-traumatic micro-serrefine clamp was placed on the renal artery and vein and 50 μL of AAV vector solution was injected using a 31-gauge needle (328468, BD Medical, Franklin Lakes, NJ). Ischemic time required for the injection is less than 1 min ...
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bioRxiv - Immunology 2023Quote: ... Following surface staining cells were fixed and permeabilized using a BD Cytofix/ Cytoperm Fixation/ Permeabilization Solution kit (Cat# 554714 BD Biosciences, Franklin Lakes NJ) followed by intracellular staining with anti-IFNγ ...
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bioRxiv - Cell Biology 2022Quote: ... Cells grown on 96 well plates were fixed and permeabilized for 20 mins at 4°C with Fixation/Permeabilization solution (BD Biosciences, cat. no. 554714). Cells were washed 3 times in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in 100 μl of staining solution of FACS buffer containing biotinylated antibodies against CD31 (BD Biosciences, 558737, 1:100), CD45 (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... After 19h incubation at 37°C, the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville, MD) and fluorescence was evaluated on a LSRII (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean fluorescence intensity (MFI ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight in 0.1M PBS + 0.1% TX-100 solution containing primary antibody for rat anti-CD31 (label endothelial cells; 1:200 dilution, BD Clone MEC 13:3: #553370) or sheep anti-CD93 (label endothelial tip cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then washed twice with PBS and incubated with propidium iodide staining solution (2.5 μg/mL PI (BD Biosciences, Franklin Lakes, NJ, USA), 100 µg/mL RNAse A (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... 200 µL of PI (from 50 µg/ml stock solution) was then added and the cell cycle was analyzed by flow cytometry (BD FACSCanto™ II, BD Bioscience) using untreated cells as negative control for gating ...
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bioRxiv - Microbiology 2024Quote: ... Samples were washed with FACS buffer and mammalian cells were permeabilized for 20 minutes at 4°C with BD Fix/Perm solution (BD Biosciences, Franklin Lakes, NJ, USA) and washed with 1X BD Perm/Wash buffer (BD Biosciences) ...