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Citations for Becton, Dickinson and Company :
501 - 550 of 703 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 20 μL of this cell solution was combined on ice with 40 μL of Basement Membrane Matrigel (BD Biosciences) and plated in the center of a 24-well tissue culture plate ...
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bioRxiv - Immunology 2020Quote: ... The cells were wash two times in PBS and fixed in suspension using the solution (missing ref; BD Biosciences) with 4% PFA ...
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bioRxiv - Immunology 2020Quote: ... cells were washed in perm/wash solution and resuspended in 0.5% PFA-FC stain buffer prior to running on a Symphony A5 flow cytometer (BD). Analysis was performed using FlowJo software ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... cells were resuspended in Fix/Perm Solution for 20min at 4°C to fix and permeabilize the cells (BD Bioscience Cytofix/Cytoperm Cat # ...
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bioRxiv - Microbiology 2021Quote: ... 0.6 g of MgSO4·7H2O, 0.1 g of CaCl2 and 1.8 ml of 5 x M9 minimal salts solution [BD Biosciences]). Individual cell suspensions were serially diluted in soil buffer and stained with SYBR Green I for 15 min in the dark ...
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bioRxiv - Microbiology 2021Quote: ... and fixed and permeabilised using Fixation/Permeabilization Solution Kit according to the manufacturer’s instructions (BD Biosciences, San Jose, U.S.A.). Cells were stained with a mouse anti-D8 monoclonal antibody AB1.1 (108 ...
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bioRxiv - Cancer Biology 2021Quote: ... stimulation with GolgiStop solution being added for a total of 5 hours block intra-cellular protein transport (BD Bioscience). As a positive control for cytokine production ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice in wash buffer and residual RBC were lysed using BD FACS Lysing Solution (BD Biosciences) at room temperature for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII analytic cytometer (BD Bioscience).
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bioRxiv - Cancer Biology 2020Quote: ... 1 x 104 cells were washed one time with PBS and fixed with CytoFix/CytoPerm solution (BD Biosciences. USA) for 10 min on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... then Fc-receptors were blocked in Fc-blocking solution for 10 minutes at room temperature (BD, San Jose, CA). Sections were further blocked with with 3% goat serum in TBST for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... namely: a 16 μg/ml colistin solution which was prepared by elution of eight 10 μg colistin disks (BD) in 5 ml volume of CA-MHB (BD) ...
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bioRxiv - Immunology 2021Quote: ... These membranes were coated with rat-tail collagen I solution (50 μg/mL) (BD Biosciences, San Diego, CA, USA) and placed in a 12-well plate ...
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bioRxiv - Microbiology 2021Quote: ... 400 μL of binding buffer was added to the solution and further analyzed by FACScallibur flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with BD Cytofix/Cytoperm and permeabilized with BD Perm/Wash solution (cat# 554714, BD Pharmingen), stained with anti-IgG(H+L)-a350 (Polyclonal ...
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bioRxiv - Microbiology 2023Quote: ... and underlain beneath a fresh BG-110 solid medium plate containing 1.5 % agar solution (Becton, Dickinson and company, USA) with a bottom dish glass ...
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bioRxiv - Biochemistry 2023Quote: ... blocked cells were pelleted and resuspended in 100 µL of primary antibody solution (1:100 mouse-α-BrdU [BD Bioscience ...
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bioRxiv - Immunology 2023Quote: ... The pellet was resuspended with 10 ul Fc block solution (1:10 Fc block) (BD Biosciences, New Jersey, USA) for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell preparation for intracellular staining of sorted cells was performed with BD Cytofix/Cytoperm according to manufacturer’s recommendations and incubated with anti-Ki67-FITC and CDK6-AF647 in PermWash solution (BD) overnight ...
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bioRxiv - Immunology 2024Quote: ... Cells were then centrifuged (350g, 5 minutes) and resuspended in 1X Red Blood Cell Lysis solution (555899, BD Bioscience) for 15 minutes at RT ...
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bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Cytokine production of T cells was evaluated by surface and intracellular staining using Fixation/Permeabilization Solution Kit (BD Biosciences). Background cytokine staining was subtracted ...
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bioRxiv - Neuroscience 2023Quote: ... Detection was accomplished by incubating with anti-ATXN2 primary antibodies (diluted 1:500 in blocking solution, BD Biosciences 611378) overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and then sequentially incubated in blocking solution (PBS, 0,5% Tween 20,1% BSA) containing anti-BrdU primary antibody (1/100, BD Biosciences) and anti-mouse secondary antibody coupled to FITC (1/300 ...
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bioRxiv - Immunology 2024Quote: ... Isolated cells were centrifuged (350g, 5 minutes) and resuspended in 1X Red Blood Cell Lysis solution (555899, BD Bioscience) for 15 minutes at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... dissociated cell clusters were fixed for 20 min at 4°C in cold BD fixation/permeabilizationTM solution (BD Bioscience). Cells were washed twice in BD Perm/WashTM Buffer (BD Bioscience ...
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bioRxiv - Immunology 2022Quote: ... Red blood cell lysis (RBC) was performed by adding 3 ml of 1x FACS lysing solution (BD Biosciences, 349202), followed by vigorous vortexing for 30 sec and incubation for 10 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Cells were fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII (BD Bioscience, San Jose, CA).
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bioRxiv - Microbiology 2023Quote: ... and underlain beneath a fresh BG-110 solid medium plate containing 1.5% agar solution (Becton, Dickinson and company, USA) with a bottom dish glass ...
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A balance between pro-inflammatory and pro-reparative macrophages is observed in regenerative D-MAPSbioRxiv - Bioengineering 2023Quote: ... These solutions were filtered through a 0.22 μm sterile filter (Argos Technologies) before loading into 1 ml syringes (BD). The aqueous solutions did not mix until droplet segmentation on the microfluidic device ...
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bioRxiv - Physiology 2024Quote: Peripheral blood from WT and Mrp14-/- mice was harvested and erythrocytes were lysed with lysing solution (BD FACS™). Samples were stained for CD18-FITC (5μg mL-1 ...
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bioRxiv - Microbiology 2024Quote: ... PM-WCPV-S_RBD-1) was from JPT innovative peptide solution (Berlin, Germany). GolgiStop (Catalog no. 554724) was from BD bioscience (New Jersey ...
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bioRxiv - Microbiology 2024Quote: ... 0.6 g of MgSO4·7H2O, 0.1 g of CaCl2 and 1.8 mL of 5 x M9 minimal salts solution [BD Biosciences]) and an aliquot was taken for cell counting by flow cytometry (see below) ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were fixed and stained for internal markers with permeabilization solution according to manufacturer’s instructions (BD Cytofix/CytopermTM). Flow cytometry was performed using an Attune NxT Flow Cytometer (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining required additional steps after surface staining using the BD Cytofix/CytopermTM Fixation/Permeabilization Solution Kit (BD Biosciences) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... the following solutions were prepared: 1) Cell labeling buffer made of BD Stain Buffer (FBS) (BD, Cat No. 554656) with 0.1 g/l sheared herring sperm DNA (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were fixed and stained for internal markers with permeabilization solution according to manufacturer’s instructions (BD Cytofix/CytopermTM). Flow cytometry was performed using an Attune NxT Flow Cytometer (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... Phenol Red-free basement membrane extract (BME)/Matrigel was obtained as an 8.9-10 mg/ml solution from BD Biosciences (San Jose ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 100 μl PBS/5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... DNA denaturation was achieved with 0.5% BSA and 2M HCl after which cells were incubated in 1:2000 solution of anti-BrdU (BD Pharmingen) for 1h at RT ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were resuspended with a 0.5% BSA/PBS solution in a FACS tube and analyzed with a FACSAria IIIu Cell Sorter (BD Biosciences), recording 20,000 events for each sample ...
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bioRxiv - Biochemistry 2020Quote: ... The resins were pre-treated with yeast extract solution (0.1 g/100 ml H2O for 1 hour; BD Biosciences, NJ) to avoid non-specific binding of EVs to resins ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were further incubated for 1 hr in solution containing goat anti-rabbit antibody conjugated with FITC (BD Biosciences, USA). All steps were carried out at room temperature ...
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bioRxiv - Genetics 2022Quote: ... the majority of each sample was then resuspended and fixed in Cytofix/Cytoperm solution (100 μL/sample, BD Bioscience, 554722) following manufacturer’s instructions and washed in Perm/Wash buffer (2 mL/sample ...
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bioRxiv - Immunology 2022Quote: ... Intracellular cytokine staining was performed by activating cells for 4-5h at 37°C with the Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) and subsequent fixing and staining per manufacturer’s instructions using the BD Fixation/Permeabilization Solution Kit (BD Biosciences). For intranuclear transcription factor staining ...
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bioRxiv - Pathology 2021Quote: ... Whole blood samples were then lysed and fixed in 2 ml of FACS Lysing Solution (BD Biosciences, San Jose, CA) for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... single cells were washed three times in FACS buffer (0.1% (w/v) BSA in DPBS) and then fixed and permeabilized with Cytofix/Cytoperm Fixation/Permeabilization Solution (BD Biosciences) for 20 min at 4 °C ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... The 100 μl of the resuspended solution was incubated with 5 µl of FITC Annexin V (BD Pharmingen™, 560931) in FACS tube for 15 min at RT (25°C ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA, and BD OptEIA Substrate Solution ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA,and BD OptEIA Substrate Solution ...