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Citations for Becton, Dickinson and Company :
651 - 700 of 710 citations for 3 Acetyl N ethylpyridinium bromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: Zebrafish wild-type and vsxKO retina sections from different developmental stages were analysed for the detection of apoptotic and mitotic cells using rabbit anti-active caspase-3 antibodies (BD Biosciences, 559565) and rabbit anti-phospho-histone H3 antibodies (Merck Millipore ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for surface markers (when required) then fixed/permeabilized by using PE Active Caspase-3 Apoptosis Kit (BD Bioscience, 550914) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Between 2 - 4x105 events were collected per sample within 3 hours after staining on an LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Mammary fat pad injections were performed in the 4th pre-cleared mammary fat pad of 3 weeks old female mice in 50% matrigel (BD Biosciences, Germany). Breast or prostate cancer-origin of xenografts were verified by a pathologist.
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Lentivirus was titrated by serial dilution of lentivirus and incubating with 5000 HEK293T cells in 96 well plates with 8 µg/ml polybrene for 3 days followed by GFP detection by flow cytometry (BD Biosciences LSRFortessa). Transduction units (TU ...
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bioRxiv - Immunology 2022Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... PBMC from the 3-time point from each patient were labelled using Single Cell Labelling with the BD Single-Cell Multiplexing Kit (BD Biosciences, #633781) and 52 different BD AbSeq Ab-Oligos reagents following the manufacturer’s protocol (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were suspended in 500 μl PBS with 3 % FBS and then detected by flow cytometry (BD Biosciences, San Diego, CA, USA).
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bioRxiv - Neuroscience 2020Quote: COs were washed 3 times for 5 minutes in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...
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bioRxiv - Immunology 2020Quote: ... and 3-Iodo-L-tyrosine-treated B cells were labeled by using the Annexin V Fitc Apoptosis detection kit (BD, Heidelberg, Germany) according to manufacturer`s instructions ...
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bioRxiv - Immunology 2022Quote: T cells were isolated as described above from the intestine of 3 individual samples and PLZF+ CD4+ TCR-β+ T cells were sorted on proxy surface markers using FACSAria2 SORP (BD Biosciences) as previously described (14) ...
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bioRxiv - Molecular Biology 2022Quote: ... The dermis was incubated in the enzyme mix from the whole skin dissociation kit (Milteney Biotec) for 3 hours according to the manufacturer’s instructions and further processed by Medicon tissue disruptor (BD Biosciences, Stockholm, Sweden). The dermal cell suspension was incubated with CD90 Microbeads ...
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bioRxiv - Cell Biology 2022Quote: ... maintained for 20 min on ice and then homogenized with syringe every 5 min (BD Microlance™ 3 Needle 0,5 × 25 mm). Nuclear lysate supernatants were collected at 1000 rpm for 10 min at 4°C 43.
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bioRxiv - Physiology 2022Quote: ... Venous peripheral blood (4-6 ml for adults, 1- 3 mL for children and infants) was collected on-site on EDTA (BD vacutainer©) and immediately stored at 4-8°C until use ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was carried out with the following antibodies diluted in BD Perm/Wash buffer: Active-caspase-3-GranzymeB-PE (C92-605, BD, cat #561011), Active-caspase-3-BV650 (C92-605 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were stained for 30 min on ice with 5 μl of biotin-labeled anti–cleaved caspase-3 monoclonal antibody (BD Biosciences, #550821). The cells were washed in Perm/Wash buffer (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... N2a-ssGT cells were imaged for GFP fluorescence using CytationTM 3 Cell Imaging Multi-Mode Reader (BioTekTM) and flow cytometry analysis by Accuri® C6 flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2024Quote: ... and HIV-1 NL4-3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
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bioRxiv - Microbiology 2024Quote: ... as well as HIV-1 NL4.3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
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bioRxiv - Cancer Biology 2024Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and the data was analyzed using FlowJo software (BD Biosciences, RRID:SCR_008520). All listed antibodies for flow cytometry are from Biolegend ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H: BD construct #8) and each column is a single AD construct (For example column 1 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was weighed then homogenized for 3 minutes in a bullet blender and serum was added to a serum separator tube (BD Microtainer: 365967) before being spun down and diluted 1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Unspecific binding of antibodies was blocked by incubating the samples with 3% rat serum in BD staining buffer (BD Biosciences, Heidelberg, Germany) for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and VC3ai cleavage by caspase-3/7 was assessed based on fluorescence emission at 530 nm using a BD LSR Fortessa™ Cell Analyzer (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... cells were suspended in 0.5 mL PBS+3% FBS and stained for flow cytometry analysis by adding anti-CD16/CD32 antibody (BD Pharmingen; clone 2.4G2) ,anti- CD117 (Biolegend clone 2B8) ...
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bioRxiv - Neuroscience 2024Quote: ... Tracheal intubation was performed after sevoflurane exposure (5%, 3 minutes) using a 20-gauge intravenous catheter (REF 382434; BD Angiocath Plus, Singapore). To ensure proper positioning of the catheter so as to avoid one- lung ventilation and gas leakage during ventilation ...
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bioRxiv - Bioengineering 2024Quote: The expression of the exhaustion markers PD-1 and TIM-3 was evaluated using anti-PD1 (cat. 560795, BD Biosciences, BD PharMingen) and anti-TIM3 (cat ...
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bioRxiv - Molecular Biology 2021Quote: ... Between 2 - 4×105 events were collected per sample within 3 hours after staining on a LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... by subcutaneous injection of 3 × 105 A375 or H2122 cells in RPMI-1640 medium supplemented with 50% Matrigel (BD Biosciences, cat. no. 354234). Seven days after the injection ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were immediately split into 3 fractions: 2 ml were transferred to heparin and EDTA tubes respectively (both BD, Franklin Lakes, New Jersey). Heparin samples were then centrifuged (1000x g ...
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bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
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bioRxiv - Immunology 2020Quote: Female C57BL/6J mice aged 8 – 12 weeks were injected intraperitoneally with 2 ml each of 3% sterile thioglycollate (BD Biosciences, catalog # 211716) medium ...
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bioRxiv - Immunology 2021Quote: ... were resuspended in defRPMI-1640 with 3% newborn calf serum and Benzonase™ (FACS buffer) in 5 mL FACS tubes and pre-incubated with GolgiStop™ (BD Biosciences) for ∼60 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... were plated around the rim of the well of a 96-well plate in a 4:3 mixture of Matrigel and MammoCult medium (BD Bioscience CB-40324). The cell mixture was then incubated at 37°C with 5% CO2 for 15 min to solidify the gel ...
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bioRxiv - Systems Biology 2022Quote: Single cell suspensions were plated around the rim of the well in the 3:4 mixture of Mammocult and Matrigel (BD Bioscience CB-40324). We seed 5’000 cells/wells for mini-rings in white 96-well plates (Corning #3603 ...
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bioRxiv - Neuroscience 2022Quote: ... Following overnight incubation the plate was washed 3 times with DPBS and then incubated with 20 µl anti-CD45 antibody (BD Pharmingen, catalog #550539) in 12 ml of 0.2% BSA for a minimum of 2 hours ...
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bioRxiv - Immunology 2023Quote: ... Mice were inoculated subcutaneously (50uL) with 3×104 PFU or injected intracranially (10uL) with 50 PFU of LGTV-TP21 using insulin syringes (BD Medical, #BD-329461). At appropriate times post infection ...
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bioRxiv - Cancer Biology 2023Quote: ... of both preparations was determined by titration onto target cell lines and quantification of the percentage of BFP+ cells 2–3 days post-transduction by flow cytometry (BD Biosciences LSR II).
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bioRxiv - Microbiology 2023Quote: ... and allowed to gel over a 1-mL layer of acellular collagen in six-well culture inserts with 3-μm-pore polycarbonate filters (BD Biosciences NJ, USA). Gels were allowed to contract for 4–5 days before seeding with 3×105 HaCaT keratinocytes in serum and mitogen-supplemented DMEM/F12 raft medium ...
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bioRxiv - Pathology 2023Quote: ... The culture medium was sampled by adding 1-3 drops to microscope slides followed by Gram stain (BD BBLTM Gram Stain Kits; B12539) and imaging.
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bioRxiv - Physiology 2021Quote: ... Sections were then washed with PBS 3 times for 15 min and incubated with PBS containing Hoechst (1:5000, #561908, BD Pharmingen, Lincoln Park, NJ) for 5 min before a final series of washes with PBS.
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bioRxiv - Immunology 2022Quote: ... and F15 NOD Nfκb1 heterozygote were suspended in biotin-free RPMI containing 0.1% azide and 3% FCS and surface stained in 96-well plates with the 10–3.6 PE (anti-I-Ag7) (BD PharMingen, Franklin Lakes, NJ, USA), which is class II major histocompatibility complex (MHC ...
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bioRxiv - Cancer Biology 2024Quote: ... The phosphorylation-SMAD2/3 ability of CAR T cells was detected using PE Mouse anti-Smad2/Smad3 antibodies (clone O72-670, BD Biosciences, Franklin Lakes, NJ) following the staining protocols ...
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bioRxiv - Microbiology 2022Quote: ... oxysporum conidia were isolated after growth for 3-4 d in potato dextrose broth (PDB; Becton, Dickinson and Company, Le Pont de Claix, France) by filtering through two layers of Miracloth® (Merck Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... so that the microfluidic tubing (Scientific Commodities,Inc., BB31695-PE/3) can connect the device to the sample sources in a syringe (BD 1 mL, NJ, USA).
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bioRxiv - Bioengineering 2023Quote: ... pneumoniae serotype 3 (Sp3) (ATCC; Manassas, VA) was cultured on plates of BBL Trypticase Soy Agar with 5% sheep blood (BD Trypticase Soy Agar II) (BD Biosciences ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight in 0.1M PBS + 0.1% TX-100 solution containing primary antibody for rat anti-CD31 (label endothelial cells; 1:200 dilution, BD Clone MEC 13:3: #553370) or sheep anti-CD93 (label endothelial tip cells ...
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bioRxiv - Pathology 2024Quote: ... phosphorylated TDP-43 (clone 11-9, 1:2000, CosmoBio, Tokyo, Japan) and anti-p62 (3/P62 LCK LIGAND, 1:500 BD Company, NJ, United States) were employed ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... analysis of sub-G0/G1 DNA content as previously described (23) and by measurement of active caspase-3 by flow cytometry using a commercial kit (BD Biosciences, Franklin Lakes, NJ).
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bioRxiv - Genomics 2020Quote: ... each anaesthetised snail was injected with 170 μL of snail saline (see Standen 1975) in the head-foot using a syringe and needle (0.45 mm × 16 mm BD Microlane™ 3, New Jersey, USA). This treatment examined the effect of wounding in activating immune defence when compared to anaesthesia without exposure to immune elicitors (treatment 2 above) ...