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Citations for Becton, Dickinson and Company :
551 - 600 of 710 citations for 3 Acetyl N ethylpyridinium bromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: The sorted cells were collected in 36 (3 samples × 12 bins) 5-mL polystyrene round-bottom centrifuge tubes (BD Falcon), each of which contained 500 μL of PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed and resuspended in PBS + 3% FCS for cell sorting on the BD FACS Aria Fusion (BD Biosciences) at the NIHR Cambridge BRC Cell Phenotyping hub ...
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bioRxiv - Immunology 2023Quote: ... and 3 μg/ml anti-IL-4 in the presence of plate bound anti-CD3ε (1 μg/ml) (BD Biosciences) and anti-CD28 (3 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 3 hours at 37°C with 5% CO2 in R10 buffer supplemented with 0.33 μl/ml GolgiStop (BD Biosciences) and 15 μg/ml DNase I ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed by adding 200 μl of DNase-free water and resuspending them up and down using a 3 ml syringe and 0.5 mm needle (BD Biosciences). An equal volume of phenol:chloroform:isoamyl alcohol (25:24:1 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After 48h cultures were diluted and plated on Nutrient Agar plates (5 g/L peptone BD difco, BD Bioscience; 3 g/L yeast extract BD difco ...
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bioRxiv - Microbiology 2023Quote: ... The cell pellets from each isolate were then resuspended in 1 ml of T-R minimal medium containing no carbon source and injected into the IsoCaRB system simultaneously using a 3 ml syringe (BD Biosciences #309657 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells (3 × 105) in 300 mL of serum-free medium were seeded into FluoroBlock TM Cell Culture inserts (BD Falcon). Serum free media with 100 ng/mL LOX ...
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bioRxiv - Neuroscience 2023Quote: ... we created a dural eyelet by inserting a short guide tube (3-4 mm length of 27G hypodermic needle, BD) 1-2 mm into the dura and then carefully threading the probe through the eyelet under magnification ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After 48h cultures were diluted and plated on Nutrient Agar plates (5 g/L peptone BD difco, BD Bioscience; 3 g/L yeast extract BD difco, BD Bioscience ...
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bioRxiv - Bioengineering 2024Quote: ... and NANOG was conducted with the following primary antibodies respectively: anti-Oct-3/4 (BD Biosciences, San Jose, CA, USA), rabbit anti-Sox-2 (New England Biolabs) ...
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bioRxiv - Immunology 2024Quote: ... Cells were analysed on a 3 laser (405nm, 488nm, and 640nm) BD LSR Fortessa X-20 flow cytometer (BD Biosciences). Medium or high flow rates were used ...
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bioRxiv - Immunology 2024Quote: ... and stored in FB 4C overnight before analysis on a 3 laser Aurora spectral flow cytometer (Cytek, figure 1D, Supplementary Figure 2) or X20 Special Order 5-lasers Fortessa (BD) flow cytometer (other figures) ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibodies for immunostaining were diluted in PBS with 3% BSA and 0.1% Tween-20 as follows: 1:800 rat anti-mouse CD31 (BD, 553370), 1:1000 rabbit anti-ERG (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... The infection was carried out using a KDS 100 automated syringe pump (KD Scientific) and a 1 mL syringe (Terumo) with a MicrolanceTM 3 needle (BD). Haemocoel was injected with 10 μl of S ...
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bioRxiv - Neuroscience 2024Quote: ... stained spinal cord cell suspensions were sorted for PL13 and DAPI-positive cells using SORPAria or Aria 3 cell sorter (BD). The analysis software used was FlowJo ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed twice with R10 to remove any remaining viral particles and sorted for mCherry-positivity on a FACSAria 3 cell sorter (BD). The heterogeneous population was then assayed for successful editing by sequencing the genomic DNA flanking the Cas9 cut site ...
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bioRxiv - Immunology 2024Quote: ... Conditioned media was collected from untreated or poly(I:C) treated+24h recovered cells using a 3 ml syringe (BD Syringe) with a 0.22 μm PVDF syringe filter (Millipore #SLGVM33RS ...
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bioRxiv - Genomics 2024Quote: ... Sca1/Pe-Cy7, Flt-3/PE, CD48/FITC, CD150/BV711, Lineage (CD4, CD8, Gr1, Mac1, Ter119)/Pacific Blue and purchased from BD Biosciences or eBioscience.
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bioRxiv - Immunology 2024Quote: ... as positive markers for MSCs and CD3/4/8 (CD4/8/3, BD Tritest™; BD Biosciences San Jose, CA) CD56 (NCAM16.2 ...
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bioRxiv - Immunology 2024Quote: ... as positive markers for MSCs and CD3/4/8 (CD4/8/3, BD Tritest™; BD Biosciences San Jose, CA) CD56 (NCAM16.2 ...
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bioRxiv - Immunology 2024Quote: ... the cells were cultured for 2-3 days and stained with corresponding antibodies to sort knocked-out cells using a FACS Aria flow cytometer (BD).
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bioRxiv - Biochemistry 2024Quote: ... Immunostainings were performed using the following antibodies: rat anti-mouse Mac-3 monoclonal antibodies (550292; BD Pharmingen, San Diego, CA)and Cleaved Caspase-3 rabbit monoclonal antibodies (9664 ...
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bioRxiv - Bioengineering 2021Quote: ... Infected cells grew for 3 days and then infection efficiency was measured using flow cytometry to measure EGFP (BD Accuri C6); the dose that gave rise to 5 - 15% EGFP+ cells was selected for each LSR for further experiments ...
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bioRxiv - Immunology 2022Quote: ... 1–3 ×106 PBMC were stained with a saturating concentration of the following antibodies: CD3 AF700 (clone SP34-2, BD Biosciences), CD4 eFluor450 (clone OKT4 ...
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bioRxiv - Microbiology 2022Quote: ... BW25113ΔompT (JW0554) and BW25113ΔeptA (JW5730) (Supplementary Table 1) were routinely grown aerobically in Difco Antibiotic Medium 3 (BD, Franklin Lakes, USA) at 37 °C degrees without agitation ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in cold phosphate-buffered saline (PBS) containing 3% FBS and stored at 4°C until analysis using a LSRII flow cytometer (BD Biosciences). FlowJo software v10 (Treestar ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cells were either stained with antibody dissolved in PBS containing 3 % albumin (surface markers) or permeabilized with Perm/Wash buffer (BD Biosciences) according to manufacturer’s instruction and stained with the respective antibody (mix ...
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bioRxiv - Immunology 2020Quote: ... then cultured in complete RPMI media (3×106 cells/mL) plated on anti-CD3 (145-2C11; BD Pharmingen, San Diego, CA) antibody-coated tissue culture plates ...
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bioRxiv - Immunology 2020Quote: ... cover slips were washed 3 times in 1xPBS and incubated with secondary antibodies (anti-rabbit-AF647, anti-mouse-AF488 (BD Bioscience)) diluted 1:1000 in antibody diluent for 1 hour at RT ...
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bioRxiv - Biophysics 2021Quote: ... 1 mL plastic syringes (BD PlastipakTM; sterile needles, 25G x 1” – NR. 18, 0.5 mm x 25 mm, BD MicrolanceTM 3), and portex tubing PE (Scientific Laboratory Supplies ...
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bioRxiv - Bioengineering 2020Quote: ... cells were then suspended in alginate precursor solution ‘A’ at 5 x 106 cells/mL (final concentration 2.5 x 106 cells/mL) and loaded into 1 or 3 mL syringes (BD luer lock). Following previous protocols ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed with PBS containing 5 mM EDTA and 3% FBS (FACS Buffer) and then blocked with 1% anti-mouse CD16/CD32 (BD biosciences), 5% normal rat serum and 5% normal mouse serum in FACS buffer for 10 minutes at RT ...
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bioRxiv - Plant Biology 2021Quote: ... A 1.0 mL aliquot consisting of approximately 108 cells was inoculated in a 100 mL 3% tryptic soy broth (TSB, BD-Difco) in Erlenmeyer flasks ...
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bioRxiv - Microbiology 2020Quote: ... AU) of the infected cells 1 and 3 hours after infection by flow cytometry using a FACS Aria III (BD Biosciences). The intensity of GFP fluorescence corresponds to the amount of intracellular bacteria ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining using antibodies detecting Active Caspase-3 (C92-605) and BCL2L11 (Y36) was performed using BD Cytofix/Cytoperm (BD Biosciences) or eBioscience FoxP3/Transcription Factor Staining Buffer Set (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... 20µL were transferred into 180µL of 10mM Tris-1mM EDTA (for DiOC2(3) staining) or PBS (for DiSC3(5) staining) and analyzed by flow cytometry using LSR Fortessa (BD Bioscience) operated by FACS Diva software (BD Bioscience) ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were run for 5 min at low flow rate (12 ± 3 μl min−1) and cytometric data were documented using BD CellQuest Pro software (BD Biosciences). In addition to glaucophyte cells ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 3 million MDA-MB-231-GFP-Luc cells were suspended in the mixture of PBS: Matrigel (BD Biosciences) at 1:1 ...
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bioRxiv - Immunology 2022Quote: ... ex vivo-stimulated cells were stained with cell surface markers followed by staining with anti-cleaved caspase-3 antibody (C92-605; BD Biosciences) for 45 minutes at 4°C after processing with Fixation/Permeabilization kit (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... and plated on the upper chamber of the 24-well format HTS FluoBlokTM Multiwell Insert System (3 μm pore size; BD Falcon) in RPMI without phenol red (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... with the relevant peptides (each at 1 μg/ml) in the presence of anti-CD28 (clone L293) and anti-CD49d (clone L25) (each at 3 μl/ml; BD Biosciences). Cells were analyzed by flow cytometry after incubation for 24 hr at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and the fraction of sgRNA-expressing cells was measured 3 days and 5 days after treatment as BFP-positive cells by flow cytometry on an LSR-II (BD Biosciences). Specifically ...
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bioRxiv - Immunology 2021Quote: ... the wells were washed six times with PBS containing 0.05% (v/v) Tween-20 (PBS-T) and blocked with 3% (w/v) Difco Skim Milk (BD Biosciences, USA) in PBS-T for 2 h in agitation at RT ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for 3 days at 37 °C with 1:100 dilutions of BV421-labeled mouse anti-human CD35 clone E11 (BD Biosciences) and Alexa Fluor 488-labeled mouse anti-Ki67 clone B56 (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... the samples were rinsed 3 times in FACS buffer and then suspended in FACS buffer for analysis and sorting with FACSARIA III (BD Biosciences). Living single cells were selected via FSC/SSC isolation.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were analysed on the BD LSR Fortessa X-20 or BD FACSymphony or sorted on the BD FACSAria Fusion 5 or BD FACSAria Fusion 3 (BD Biosciences). Data was analysed using FlowJo (v 10.4).
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bioRxiv - Immunology 2022Quote: Igλ-enriched B1-8 cells were cultured for 2-3 days at 4 *105 cells/well in a 12-well tissue culture plate (BD Falcon) and then washed ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of iron in LB was calculated using specifications provided in the “Bionutrients Technical Manual Vol 3” supplied by the manufacturer (BD Biosciences). For assays testing the role of iron availability ...
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bioRxiv - Microbiology 2020Quote: ... NJ) were coated for 3□hours with 10□μg of Matrigel following the manufacturer’s instructions (Biocoat, BD Biosciences, San Jose, CA). Inserts were placed into 24-well dishes containing 500□μl of RPMI medium supplemented with 1% fetal calf serum ...