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Citations for Becton, Dickinson and Company :
6751 - 6800 of 8729 citations for Elongator complex protein 1 ELP1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Disaggregated cells and organoids were resuspended in 150 - 200 µl of a 1:1 v/v mixture of cold DMEM/F12: Matrigel (BD Biosciences, San Jose, CA, USA). 8-12- week-old mice were anesthetized with isoflurane and the suspension was transplanted under the skin on the left flank using a 1 mL syringe and 21-gauge needle ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... Reactions were fixed with 40 µL of 1% glutaraldehyde/BD buffer for 3 min and diluted with 800 µL BRB80 (BD buffer without 1 mM DTT). Fixed and diluted samples were added to spin down tubes containing a removable chock ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in a binding buffer containing 1 μg/mL propidium iodide (PI) and 1 μg/mL APC-labeled annexin V (BD Pharmingen, San Diego, CA, USA). All specimens were analyzed by flow cytometry (FACSCalibur ...
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bioRxiv - Bioengineering 2023Quote: ... cells were diluted to a final concentration of 1 × 107 cells mL− 1 in staining buffer (fetal bovine serum) (BD Pharmingen, Franklin Lakes, NJ, USA); 2.5 µL of fluorescence-labeled antibodies was added to 50 µL of cell suspension ...
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bioRxiv - Neuroscience 2024Quote: ... 1:4000 polyclonal rabbit anti-cFos, Merck ABE457, lot numbers 2935662/2967893, BrdU: 1:100 monoclonal mouse anti-BrdU, BD Biosciences B44, lot number 9172603) was then applied overnight at room temperature within 3% serum in buffer ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed twice with PBS as described above and the cell pellet was resuspended into 25 µl of FACS buffer (1% FBS in PBS) containing human FC block (BD Bioscience, # 564219; 1:200 dilution) and either 200 nM MLi-2 (synthesised by Natalia Shpiro ...
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bioRxiv - Immunology 2024Quote: ... About 40,000–60,000 cells (1:1 ratio of epithelial to lamina propria cells) from each sample were loaded into the BD Rhapsody™ Cartridge (BD Biosciences; Cat. No. 633733). According to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... to detect neuron-specific class III ß-tubulin in nerve and rat anti CD31/PECAM-1 to detect platelet endothelial cell adhesion molecule-1 as a pan-endothelial marker of vessels (BD Biosciences, U.S.A., Cat. No. 553369). The next day ...
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bioRxiv - Cancer Biology 2021Quote: ... Xenograft tumors of the human melanoma cell lines WM3734 or WM3734Tet3G-KDM5B were generated by injection of 2x105 cells in 200 µl medium [1:1 mixture of Tu2% with Matrigel® (BD Biosciences, Franklin Lakes, NJ, USA)] s.c ...
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bioRxiv - Cancer Biology 2021Quote: ... Xenograft tumors of the murine melanoma cell line CM 18 were generated by injecting 1x105 cells in 200 µl medium [1:1 mixture of RPMI medium with Matrigel® (BD Biosciences, Franklin Lakes, NJ, USA)] s.c ...
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eATP/P2X7R axis drives nanoparticle induced neutrophil recruitment in the pulmonary microcirculationbioRxiv - Immunology 2024Quote: ... QDs were resuspended in 50 μl of DPBS at a concentration of 1 pmol/g and intravenously injected into mice through the angular vein using a 1 ml insulin syringe (Becton, Dickinson and Company, Franklin Lakes, USA). Melamine resin particles (MF-FluoRed ...
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bioRxiv - Microbiology 2024Quote: ... Rifr strains were streaked from cryostocks in nutrient agar plates (NA, 8 g L-1 Difco™ Nutrient Broth, BD, and 15 g L-1 agar), and incubated for 48 h at 30 °C ...
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bioRxiv - Biophysics 2024Quote: ... transferred in a V-bottom 96-well plates and co-cultured with K562 cells (ATCC) at 1:1 effector-to-target ratio in the presence of mouse anti-human CD107a Alexa Fluor 647 (BD Bioscience, clone H4A3, 2 uL/well) for 6 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: Eosinophils of 6-12 week-old Il5-tg females and males were positivelyenriched using PE anti-mouse Siglec F antibody (562068 BD Biosciences; E50-2440) and anti-PE microbeads (130-042-401 Miltenyi Biotech) ...
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bioRxiv - Immunology 2021Quote: ... Zap70 and CD49d were evaluated by flow cytometry (antibodies in Table S2) on an LSR II flow cytometer (BD Biosciences, Franklin Lakes, NJ). Mutational status of the expressed BCR was determined by PCR from PBMC RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Edited and non-edited cells were analyzed by flow cytometry using the following antibodies: hCD45 V450 (HI30; BD Biosciences, San Jose, CA, USA), CD34 APC (561 ...
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bioRxiv - Neuroscience 2020Quote: Western analyses were run as described[33] with the following changes: Purified mouse anti-ASyn antibody clone 42 (BD Biosciences, San Jose, CA) was diluted at 1/1000 ...
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bioRxiv - Immunology 2022Quote: ... were added to the ELISpot plate containing an immobilized primary antibody to either IFN-g or IL-4 (BD Cat# 551881 and BD Cat# 551878, respectively), and were exposed to various stimuli (e.g ...
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bioRxiv - Molecular Biology 2020Quote: ... WBM cells were blocked with Fc Blocker and stained with biotinylated-Lineage antibody cocktail according to the Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit by BD (Catalog No. 560492). The stained cells were labelled with streptavidin magnetic particles and placed into BD IMagnet (BD ...
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bioRxiv - Immunology 2020Quote: ... Pellets were then stained with a panel of antibodies for 30 minutes (4°C) followed by washes in PBS before analysis by flow cytometry (BD, Franklin Lakes, NJ).
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bioRxiv - Immunology 2020Quote: ... and the purity of monocytes was evaluated by fluorescent staining with PhycoErytherin (PE)-anti-human CD14 antibody (BD Biosciences, San Diego, CA, USA) using a Floid Cell Imaging Station (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Antibody concentrations were as follows: alveolar macrophage membranes were stained using CD206 PE-CF594 (Mouse Anti-Human CD206, BD Biosciences; catalogue number: 564063) at a concentration of 4µL in a 50μL final staining volume per section ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant was removed and cells were resuspended in 50 µL of APC-conjugated anti-CXCR4 antibody (clone 12G5, BD Biosciences, catalogue number 560936) diluted 1:50 in FACS buffer (PBS + 0.5% BSA) ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with PerCP/Cy5.5 or BV421-conjugated anti–mouse CD4 rat monoclonal antibody (RM4-5; BD Biosciences, Franklin Lakes, NJ, USA). The cells were then fixed and permeabilized with Cytofix/Cytoperm reagent (BD Biosciences ...
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bioRxiv - Physiology 2021Quote: ... Following resuspension in PBS and passage through a 70μm strainer, cells were incubated in Fc block solution (Bio Rad, Cat# BUF041A) and PerCP-conjugated anti-CD11b antibody (BD Pharminogen, Cat# 550993). After PBS washes ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in 50 µL Perm/Wash buffer containing a cocktail of anti-human antibodies including IFNγ BV480 (BD, 566100, clone B27), TNFα BV605 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: T-cell degranulation was evaluated by adding an anti-CD107a antibody at the beginning of coculture (CD107a-FITC clone H4A3, BD, Catalog N° 555800) and Golgi Stop (BD ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were resuspended and stained using cell surface antibody markers preconjugated to a fluorophore: KIT (CD117, BD Pharmingen 25-1171-82, RRID: AB_469644), CD45.2 (BD Biosciences 553772 ...
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bioRxiv - Immunology 2022Quote: ... the individual antibodies used for staining were mixed with one drop of negative control beads and anti-mouse IgG beads (BD Biosciences, CA, USA). For the dead cell stain ...
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bioRxiv - Immunology 2022Quote: Surface staining: Single cell suspensions were incubated with 5% normal rat serum and rat anti-mouse CD16/CD32 antibody (BD Biosciences close 2.4G2) then stained with fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of detection antibody (IFNγ, BD Biosciences, #51-1818KA; IL-17A, Invitrogen, #88-7371-88; and IL-5, BD Biosciences, #51-1806KZ) was added at a 1:250 dilution in ELISpot diluent (eBioscience ...
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bioRxiv - Neuroscience 2022Quote: ... Following overnight incubation the plate was washed 3 times with DPBS and then incubated with 20 µl anti-CD45 antibody (BD Pharmingen, catalog #550539) in 12 ml of 0.2% BSA for a minimum of 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Samples are ultracentrifuged and pellets were resuspended and incubated onto 96 well ELISA plates containing 5ug/mL anti-syn42 monoclonal antibody (BD transduction #610-787). ELISAs were carried out on test samples run alongside serial diluted standard synthetic α-Syn ...
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bioRxiv - Immunology 2024Quote: ... The labeled cells were cultured in complete RPMI-1640 medium plus IL-2 (20 units/ml) or IL-2 and anti-CD3 antibody (1μg/ml) (BD Pharmingen (San Diego, CA), or alternatively plus IL-4 (4ng/ml ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were washed with PBS and stained for 20min at 4°C with anti-EphB2 APC conjugated antibody (BD Biosciences, Heidelberg, Germany). Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD ...
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bioRxiv - Immunology 2023Quote: ... Surface antibodies were prepared at optimal dilutions (see Supplementary Table S1) in FACS supplemented with Brilliant Stain Plus buffer (BD; Franklin Lakes, NJ), then were added to samples in blocking solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with multiple flow cytometry validated antibody cocktails (see below) and analyzed using either BD Fortessa or LSRII cytometers (BD Horizon, BD, Franklin Lakes, NJ). Data were analyzed and quantified using FlowJo v10.6 software (RRID:SCR_008520) ...
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bioRxiv - Immunology 2023Quote: ... All 5 samples were then pooled and stained for surface markers in 70μl of the antibody cocktail (Supplementary Table 2) in BD Horizon Brilliant Stain Buffer Plus (BD Biosciences, San Jose, CA) for 30 minutes at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... pre-incubated with anti-mouse CD16/CD32 (FcRII block) and then stained with the following anti-mouse monoclonal antibodies: GR1 FITC (clone RB6-8C5, BD Biosciences, RRID: AB_394643), F4/80 FITC (clone BM8 ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 4°C for 30 min with a B cell surface marker antibody cocktail (Table S11) with 10 µl Brilliant Stain Buffer Plus (BD, cat. No. 566385) diluted in PBS containing 1% BSA up to a volume of 100 µl ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... we stained cells expressing wild-type and mutant SFV glycoproteins with a mouse anti-SFV immune ascitic fluid (ATCC Cat#: VR-1247AF) or a control mouse IgG2b antibody (BD Biosciences Cat#: 557351). Staining of wild-type and mutant EEEV or SFV E3–E2–(6K/TF)–E1 was detected with a PE-conjugated donkey anti-mouse F(ab’)2 fragment (Jackson ImmunoResearch Cat# ...
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bioRxiv - Immunology 2024Quote: ... The antibodies used for stimulation were anti-human CD3 (clone UCHT1, plate bound) and anti-human CD28 (clone 28.2, BD Biosciences, San Jose, CA) at 1 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed with FACS buffer stained with antibodies to detect surface expression of CD4 (GK1.5) and CD25 (PC61) (BD Biosciences, Franklin Lakes, NJ), whereas intracellular expression of FoxP3+ was stained using an anti-Mouse/Rat FoxP3 (FJK-16s ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with antibodies to cytokines (Table S2) and samples acquired on a flow cytometer (MACSQuant VYB, BD Fortessa or Cytek Aurora). Data was analysed in FlowJo.
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bioRxiv - Physiology 2024Quote: ... then cells were washed with 1x PBS and resuspended in FACS buffer and non-specific binding to Fc receptors was blocked by incubation with anti-CD16/32 antibody (BD Pharmingen, Cat# 553141) and the FACS antibodies BV711 conjugated anti-CD11b and Pacific blue conjugated anti-CD45 antibodies (Cat# 101241 ...
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bioRxiv - Microbiology 2024Quote: ... The homogenized cell suspension was washed and resuspended in FACS buffer containing the PE-conjugated Rat anti-mouse F4/80 antibody (BD Horizon, Cat-565411) or eFluor450-conjugated anti-mouse CD11c antibody (eBioscience) ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were dissociated and stained with an A647- conjugated antibody against the target gene product CD9 (#341648, BD Biosciences, Franklin Lakes, NJ, USA). Cells were suspended in PBS and FACS sorting was done using a BD Influx (BD Biosciences ...