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Citations for Becton, Dickinson and Company :
6701 - 6750 of 8729 citations for Elongator complex protein 1 ELP1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for 15 min at 37°C before incubation with primary antibodies Alexa Fluor 647 Rat Anti-mouse CD31 (BD Pharmigen cat:563608) for CD31 cell labeling and Alexa Fluor 647 Rat IgG2a,k Isotype control (BD Pharmigen cat:557690) ...
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bioRxiv - Immunology 2020Quote: ... All antigen specific CD8 T cells were phenotypically analyzed by Flow Cytometry after surface staining using monoclonal antibodies for Thy1.1(BD clone OX-7), CD45.1 (Biolegend clone A20) ...
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bioRxiv - Immunology 2021Quote: ... Cells were filtered and cell surface staining was performed using antibodies specific for CD3ε (eBioscience, clone145-2C11) and Vγ5 (BD Pharmingen, clone 536).
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 30 min with the following cell surface antibodies: BUV661-anti-CD45 (D058-128, BD Biosciences 741657 Lot 0198214), PE-Cy7-anti-CD20 (2H7 ...
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bioRxiv - Immunology 2021Quote: ... We used the following 28 antibodies::fluorophore conjugates and clones to enumerate major immune lineages using the Cytek Aurora: HLA-DR::BUV395 (BD; G46-6), CD8::BUV496 (BD ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with goat polyclonal antibody to influenza A virus (ab20841, Abcam Inc., Toronto, ON, Canada, mouse-anti G3BP1 (BD Biosciences, 611126), Mouse anti-PABP (Santa Cruz ...
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bioRxiv - Physiology 2020Quote: ... Microtubules were allowed to grow from SVP in DMEM-F12+10% FBS for 5 days before staining with an anti-CD31 antibody (BD Biosciences, 557355). Images of microtubule outgrow from SVP were acquired with a Leica SP5-SMD confocal microscope ...
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bioRxiv - Cancer Biology 2022Quote: Cells were collected two days after initial seeding and were incubated with antibodies labeled with fluorochromes anti-CD24-PE and anti-CD44-APC (BD Biosciences, USA). Cell sorting and immunofluorescence analysis were performed using BD FACS Aria or BD FACSCanto II (BD Immunocytometry Systems) ...
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bioRxiv - Biochemistry 2022Quote: ... Splenocytes were stimulated with plate bound anti-CD3 and exogenous anti-CD28 antibodies (10μg/ml and 1μg/ml respectively; BD Biosc, San Diego, CA) for 2 days ...
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bioRxiv - Cell Biology 2022Quote: ... Single cells were filtered with a 40 µm cell strainer (Falcon, 352340) and incubated with fluorophore-conjugated antibodies CD45–PE/Cy7 (BD Biosciences, 552848), CD11b– PE/Cy7 (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Transduction efficacies were determined via fluorescence activated cell sorting (FACS) staining with TCRmu antibody (anti-mouse TCR-β-chain, FITC, BD Biosciences) against the murine-β-chain of engineered TCR-constructs in comparison to non-transduced T cell populations.
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bioRxiv - Immunology 2022Quote: ... single-cell suspensions were subjected to osmotic red blood cell lysis and incubated with a combination of antibodies: CD4-BV421 (BD Biosciences #562891), CD8-PeCy7 (BD Biosciences #561097) ...
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bioRxiv - Immunology 2022Quote: ... The Fc cell receptors were then blocked for 10 min at 4°C with an anti-CD16/CD32 antibody (10 μg/mL: BD Biosciences 553142) and the cells were resuspended in 25 μL of staining buffer ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was carried out with the following antibodies diluted in BD Perm/Wash buffer: Active-caspase-3-GranzymeB-PE (C92-605, BD, cat #561011), Active-caspase-3-BV650 (C92-605 ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were blocked with 2% BSA in 0.1% tween TBS (TBST) for 1h and immunostained with mouse anti-paxillin IgG1 antibody (BD Transduction Laboratories 610052) (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated in media containing 10 µg/mL blocking antibody (Purified Rat Anti-Human CD29 Clone Mab 13, BD Biosciences, #552828) or equivalent concentration non-targeting isotype control (Purified Rat IgG2a κ Isotype Control Clone R35-95 ...
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bioRxiv - Microbiology 2024Quote: ... Cells (1x106 cells/mL) were cultured in a 48-well plate pre-coated with anti-CD3 monoclonal antibody (BD Pharmingen, 5μg/mL), and culture supernatants were collected after 72h of stimulation and stored at -80°C until assayed for cytokine production ...
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bioRxiv - Immunology 2024Quote: ... Single-cell suspensions were stained with antibodies listed in Table S1 and acquired with a LSR Fortessa flow cytometer (BD Bioscience, US). For cell-associated autofluorescence to be removed in the indicated experiments ...
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bioRxiv - Cancer Biology 2023Quote: Flow-cytometric analysis of mouse bone marrow cells or MUTZ3 cells was done with specific antibody stainings using mouse CD11B-APC (BD, cat 553311) and mouse Gr-1 FITC (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... and with either CSF-1R blocking antibody or isotype control antibody at 50 ng/mL (cat# NBP1-43363, clone AFS98, Novus Biologicals; IgG K isotype control cat# 554682, BD Pharmingen). After 24 hours incubation ...
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bioRxiv - Microbiology 2023Quote: ... Capture and detection antibodies for human IL-6 were from the OptEIA set for human IL-6 (Cat# 55220, RRID: AB_2869045; BD Biosciences, Heidelberg, Germany) and capture and detection antibodies for human TNF were from the OptEIA set for human TNF (Cat# 555212 ...
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bioRxiv - Microbiology 2023Quote: ... and capture and detection antibodies for human TNF were from the OptEIA set for human TNF (Cat# 555212, RRID: AB_2869042; BD Biosciences, Heidelberg, Germany). Signals were detected by Streptavidin-PE (Agilent ...
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bioRxiv - Immunology 2023Quote: ... all cells were harvested from the culture plates and the cell Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cell lines were evaluated for expression of B7-H3 using B7-H3 antibody (clone 7-517, BD or clone FM276, Miltenyi).
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bioRxiv - Cell Biology 2022Quote: ... 100,000 PBMCs that had been dissolved and quantified in each media were stained with antibodies for CD3 (APC-H7, BD Biosciences (BD)) ...
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bioRxiv - Immunology 2023Quote: Splenocytes-or BM-derived cells were resuspended in sPBS and the Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). After blocking ...
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bioRxiv - Immunology 2023Quote: ... cells were stained using fixable live/dead stain for 20 minutes at room temperature and then stained with surface-expressed antibodies (Table S2) diluted in FACS buffer and 1X Brilliant Stain Buffer (BD Biosciences, USA) for 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained with anti-Annexin V antibodies and propidium iodide (PI, DNA marker dye) as per protocol supplied with kit (BD biosciences; 556547). Flow cytometry analysis was performed for annexin V+/PI-(apoptotic ...
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bioRxiv - Developmental Biology 2023Quote: ... and stained with the following monoclonal anti-mouse antibodies as described in results: GR1 FITC (clone RB6-8C5, BD Biosciences, RRID: AB_394643), F4/80 FITC (clone BM8 ...
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bioRxiv - Immunology 2023Quote: ... For the negative enrichment we used the same antibodies as for the staining but PE-labeled (Biolegend, Cat. 328109, 334105, 303105, 313203, 343605, 304058, 337004 and 305817; BD, Cat. 561433). After negative enrichment ...
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bioRxiv - Microbiology 2023Quote: ... Blots were probed for 1h at room temperature (RT) with a mouse anti-GFP antibody (Living Colors -JL-8, BD Biosciences Clontech) in TBS-T buffer (20 mM Tris ...
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bioRxiv - Immunology 2023Quote: The Antibody mix was supplemented at 10 uL with BD Horizon™ Brilliant Stain Buffer Plus (BD Biosciences, Franklin Lakes, NJ, USA) to minimize staining artifacts commonly observed when several BD Horizon Brilliant dyes are used ...
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bioRxiv - Bioengineering 2023Quote: ... for 20min at 4°C and stained with intracellular antibodies including anti-mouse IFN-γ-Pe-Cy7 and IL-2-BV605 (BD Bioscience) for 30min at 4°C ...
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bioRxiv - Genomics 2023Quote: ... or with the appropriate class control antibodies: PE Mouse anti-IgG1 κ R-PE Clone MOPC-21 (BD Biosciences; Catalog no. 559320) and Alexa Fluor® 647 Mouse anti IgG1 κ Isotype Clone MOPC-21 (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... Unspecific binding of antibodies was blocked by incubating the samples with 3% rat serum in BD staining buffer (BD Biosciences, Heidelberg, Germany) for 5 minutes at RT ...
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bioRxiv - Immunology 2024Quote: ... Content of chemokines / cytokines was quantified after washing and staining with biotinylated detection antibodies and phycoerythrin bound streptavidin by using LSR II (BD Bioscience, Germany). Calculation of each chemokine / cytokine quantity was determined by using standard curves according manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: Intracellular staining for BIM was performed with secondary Donkey anti-rabbit APC secondary antibody using BD cytofix/cytoperm kit (BD biosciences 554722) per manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Cells were washed and then stained extracellularly with antibodies (Table S1) diluted in 0.5% FACS buffer and brilliant staining buffer (BD Biosciences, Cat# 563794) for 20 mins at room temp ...
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bioRxiv - Immunology 2024Quote: ... three LNs (for patients ccp2 and ccp3) and two replicates of PBMC were stained with a panel of fluorescent antibodies: CD45-PerCP/Cy5.5 (BD 564105, clone HI30), CD38-PE (BD 555460 ...
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bioRxiv - Immunology 2024Quote: ... cells were suspended in 0.5 mL PBS+3% FBS and stained for flow cytometry analysis by adding anti-CD16/CD32 antibody (BD Pharmingen; clone 2.4G2) ,anti- CD117 (Biolegend clone 2B8) ...
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bioRxiv - Neuroscience 2024Quote: ... The Fc cell receptors were then blocked for 10 min at 4 °C with and anti-CD16/anti-CD32 antibodies (10 µg/mL: BD Biosciences 553142) and the cells were resuspended in 25 µL of staining buffer ...
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bioRxiv - Immunology 2024Quote: ... Cells were then fixed and stained with a combination of fluorochrome-conjugated antibodies using Cytofix/Cytoperm Fixation/Permeabilization Buffer (BD Biosciences, U.S.A.) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... coverslips were incubated for one hour at RT in a humid chamber with a 1X PBG mix containing an anti-BrdU primary antibody (BD Laboratories, #347580) diluted 1:20 and RQ1 DNAse (Promega ...
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bioRxiv - Microbiology 2024Quote: ... followed by 1:25000 donkey anti-human HRP-conjugated secondary antibody (Jackson Immuno Research Laboratories 709-035-149) for 30 min and detection with TMB SureBlue peroxidase substrate (BD Biosciences 555214) was measured at OD450nm.
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bioRxiv - Immunology 2024Quote: ... Cells were stained in permeabilization buffer with the indicated antibodies and analyzed on a FACSCanto II or FACSymphony A1 (BD, Mississauga, Ontario). Graphs were prepared using FlowJo.
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bioRxiv - Immunology 2024Quote: ... containing approximately 105 human CD34+ hematopoietic stem cells (HSC) as determined by FACS using a mouse anti-human CD34 antibody (BD Biosciences, 550761). CD3 depletion of human T cells was required to avoid lethal (acute ...
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bioRxiv - Bioengineering 2024Quote: NOD8.3 CD8 T cells were isolated from splenoyctes following manufacturer’s protocol (Miltenyi Biotech 130-104-075) and αCD3/αCD28 antibody (BD Biosciences 553057/ 553294) activated for 48 hours ...
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bioRxiv - Cancer Biology 2024Quote: High-affinity microplates were sensitized with monoclonal anti-cytokine antibodies (IL-6: 555220; IL-10: 555157; TNF-α: 555212; IL-1β: 557953, OptEIA, BD Biosciences) for 16-18h ...
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bioRxiv - Cancer Biology 2024Quote: ... plates were coated with anti-β1-integrin antibody mix (5 μg/ml of AIIB2 (in-house) and anti-CD29 (BD Bioscience #610468)) in TBS (50 μl per well ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were diluted in 1X PBS between 1:100 and 1:2000 and injected in the sample chamber with sterile syringes (BD Discardit II, New Jersey, USA). All measurements were performed at 23°C and pH 7.4 ...