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Citations for Becton, Dickinson and Company :
6751 - 6800 of 7984 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... washed three times with RPMI and incubated for 30 mins at 37°C in RPMI containing Dispase (BD Cornig, 1:10 dilution) and 30 μg/μl DNase I with continuous and gentle agitation on a rotating shaker ...
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bioRxiv - Neuroscience 2022Quote: ... The primary and secondary antibodies and dilutions used here were: mouse anti-BrdU 1:100 (347580; BD Biosciences, Franklin Lakes, NJ, USA), 4c4 1:200 (mouse anti-fish leukocytes ...
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bioRxiv - Microbiology 2020Quote: The mitochondrial inner membrane potential (Δψm) was determined by staining with the membrane-permeable lipophilic cationic fluorochrome JC-1 (BD Biosciences, NJ). Overnight C ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA extraction and BrdU-labelled DNA immunoprecipitation were performed with anti-BrdU antibody (40 μL of 25 μg mL−1, BD Pharmingen, #555627). Validation of BrdU immunoprecipitation was carried out using qRT-PCR on known Early (BMP1 ...
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bioRxiv - Neuroscience 2021Quote: ... Cell suspensions were stained with the following antibodies in FACS buffer (1% fatty acid-free BSA + 1mM EDTA in PBS) for 25 minutes on ice: Fixable Viability Stain BV510 (BD Biosciences, 564406), CD11b-BV421 (BioLegend 101251) ...
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bioRxiv - Immunology 2020Quote: ... and serum-blocked in 2.5% goat serum/PBS containing 1:100 dilution of Fc block (CAT # 553142; BD Biosciences, San Jose CA). For B ...
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bioRxiv - Microbiology 2020Quote: ... Each culture contained a 10 μL 4.0 McFarland (McF) bacterial cell suspension (WHO-F or WHO-X) in 12 mL gonococcal (GC) broth supplemented with 1% IsoVitaleX (BD BBL™) and vancomycin ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in blocking solution of 1% normal horse serum in PBS with 0.3% Triton X-100 and incubated overnight at 37°C as follows: mouse (IgG1) anti-CtBP2 (C-terminal Binding Protein) 1:200 (#612044, BD Transduction Labs) targeting pre-synaptic ribbons ...
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bioRxiv - Microbiology 2021Quote: ... the same protocol was followed except that of the use of biotinylated anti-Syrian hamster IgG1 /IgG2 antibodies in 1:10000 dilution (BD biosciences, USA) and Streptavidin-horseradish peroxidase 1:8000 (Thermo-scientific ...
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Mutation in the matricellular gene fibulin-4 leads to endothelial dysfunction in resistance arteriesbioRxiv - Physiology 2022Quote: ... Membranes were blocked in 5% BSA/0.1% Tween20 in 1x TBS or 5% milk/0.1% Tween20 in 1x PBS for primary antibody mouse anti-human phospho-eNOS (1:1,000, pS1177, #612392, BD Biosciences, Franklin Lakes, NJ) or mouse anti-human-eNOS (1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed the pellet was resuspended with 1 ml PBS and analyzed in a Flow Cytometer LSRFortessa (BD Biosciences, NJ, USA). Analysis of the data output was preformed via FlowJo v10.8 software using Watson model to calculate the percent of cells in each stage of the cell cycle.
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bioRxiv - Cell Biology 2022Quote: ... Hematopoietic cells were harvested from OP9-DL1 cocultures by forceful pipetting and stained on ice for one hour with the following antibodies: Pacific Blue CD4 (1:500; BD Pharmingen, 558107), BV711 CD8a (1:1000 ...
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bioRxiv - Bioengineering 2022Quote: ... HAECs were allowed to recover from passaging for 24 h after plating and were subsequently incubated with AlexaFluor-647 anti-human VE-Cadherin antibody (1:100, BD Biosciences, #561567). After 15 minutes incubation ...
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bioRxiv - Cell Biology 2021Quote: ... were fixed on ice with a solution of 1% paraformaldehyde and incubated with fluorescent antibody to platelet markers CD41a (BD Biosciences #555467), activated integrin αIIbβ3 (BD Biosciences #340507) ...
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bioRxiv - Genetics 2020Quote: ... the harvested hNC cells were subjected to fluorescence-activated cell sorting (FACS) and hNC cells which were positive to both HNK-1 (BD Biosciences #560845) and p75NTR (Miltenyi Biotec #130-091-917 ...
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bioRxiv - Genetics 2020Quote: ... 0.5 × 106 DAPI-/SSEA4+/TRA-1-60+ cells were collected for each cell line using the BD FACSAria™ II flow cytometer (BD Biosciences). Collected cells were pooled and plated at a density of 30 000 /cm2.
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bioRxiv - Evolutionary Biology 2020Quote: ... reduced to a single-cell suspension and seeded into a 96-well plate at a density ranging between 1000 – 20 c/w in mTesRTM1 supplemented with 1 nM ROCK inhibitor (BD Biosciences, 562822). Alternatively ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were allowed to dry and then hydrated and blocked for 1h at RT with 5%milk/TBS-T and incubated overnight at 4ºC with the primary antibody mouse monoclonal anti-α-Syn 1:1000 (BD Biosciences, 610787) in 5%milk/TBS-T ...
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bioRxiv - Developmental Biology 2021Quote: ... for 60 min, the sections were reacted with the primary antibody (for CD31, 1:100 rat monoclonal anti-CD31 (BD Biosciences, 550274); for Cytokeratin ...
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bioRxiv - Cell Biology 2021Quote: Cells were treated with 25uM BrdU for 1 hour and BrdU staining detected using FITC Mouse Anti-BrdU Set (BD Biosciences, 556028). Cells were resuspended in 10μg/ml 7-Amino-Actinomycin D (7-AAD ...
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bioRxiv - Cell Biology 2021Quote: ... The ZsGreen-ODC (ornithine decarboxylase) cells were previously generated by transfection of MelJuSo cells with the ZsProsensor-1 plasmid (BD Bioscience Clontech)20 ...
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bioRxiv - Immunology 2021Quote: ... For cell staining cells were first blocked with 1 μg/ml Fc-Block (mouse Fc Block, BD Biosciences, San Jose, CA, USA) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... enriched tumor-infiltrating CD8+ T cells were re-stimulated with 1 μg/ml ionomycin (IM) and 25 ng/ml phorbol myristate acetate (PMA) in the presence of GolgiPlug (BD Biosciences, USA) for 4 hours at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... and BV421 rat anti-mouse TNF-α BD Biosciences in a total of 100 μL of 1× Perm/Wash Buffer (BD Biosciences). After two washes ...
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bioRxiv - Immunology 2020Quote: Cryopreserved lymphocytes from BAL were thawed and stimulated with H1N1pdm09 (MOI 1) or medium as a control for 18 hours at 37°C prior GolgiPlug (BD Biosciences, UK) addition as per manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... The cells were blocked with 4% BSA and stained with a 1:200 (v/v) dilution of Alexa Fluor 647 Mouse anti CD247 pY142 antibody (BD Bioscience #558489) for 1 hour at room temperature ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... After washing with 1% BSA-PBS slides were incubated with secondary goat anti-rat and goat anti-rabbit biotinylated antibodies for 30 min at RT followed by 30 min of incubation with streptavidin–HRP (1:500; BD Pharmingen, 554066). For developing DAB substrate (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... before resuspending in 25 μl of 0.2µm-filtered 1% BSA/PBS containing monoclonal FITC-anti-mouse IgA (BD Pharmingen, 10 µg/ml) or Brilliant violet 421-anti-IgA (BD Pharmingen ...
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bioRxiv - Immunology 2021Quote: ... onto a 40 µM nylon cell strainer and softly mashing the organ through the cell strainer into a petri dish using the plunger tip of a 1 ml syringe (BD Plastipak 303172). Cell suspensions were filtered several times and filters were rinsed with fresh buffer to obtain a homogeneous single-cell suspension ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C and 200 rpm shaking for 12-18 hours and subsequently diluted 1:200 fold in BBL Mueller-Hinton II broth cation-adjusted (MHII, BD cat. #212322) (+ 100 μg/mL Amp for pET17b plasmid carrying strains ...
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bioRxiv - Microbiology 2023Quote: ... after which cells were stained with SYTO 16 at a final concentration of 1 μM and counted via a BD FACS Verse flow cytometer (BD, Erembodegem, Belgium). Data was analyzed using FlowJo ...
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bioRxiv - Immunology 2023Quote: ... cells were seeded in 24-well plates or CELLview Cell Culture Dish with four compartments) (USA Scientific, 5662-7870) in culture media with 1 μg/ml propidium iodide (PI, BD Bioscience, 556463) followed by calculating PI positivity on either an Inverted Nikon Ti2 fluorescence microscope with a 40X objective (N.A ...
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bioRxiv - Cell Biology 2022Quote: ... labelled with cell hashing antibodies and isolated by FACS (all steps at 4°C): Cell suspensions were incubated with Fc-block (1:50, BD Pharmingen 553142) for 10min and subsequently incubated with mouse CD45 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used in this study: rabbit anti-AP-4 epsilon 1:400 for Western blots (612019; BD Transduction Labs); rabbit anti-ATG9A 1:200 for immunofluorescence and 1:1000 for Western blots (ab108338 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were gated on FSC and SSC followed by single cell gate on FSC-HH/FSC and SSC-H/SSC and a LIVE/DEAD near IR marker (1:1000, BD Biosciences, L34976). The following antibodies were used ...
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bioRxiv - Biochemistry 2023Quote: ... cells were processed as for CDC45 but without the methanol fixation step and with a 1:1000 dilution of mouse monoclonal anti-MCM2 (BD Biosciences, 610701) for 14 hours at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... VE-cadherin was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD144 (BD Pharmingen™), 2 min prior to imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... and stained with the following conjugated antibodies (1/100 dilution in PBS) for 15 minutes at 4°C: CD44 (PE, BD Biosciences 555479), EpCAM (FITC ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then immuno-stained 1 hour at room temperature with a panel of antibodies: anti-CD3 (563725, BD, Franklin Lakes, NJ), anti-TNFα (Biolegend 502938) ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-CD28 antibodies (eBioscience, clone 37.51, 1 µg/ml) on TC plates pre-coated with anti-hamster antibody (BD Pharmingen, G94-56), and IL-2 (Immunotools ...
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bioRxiv - Cell Biology 2024Quote: ... cells were counted using a hemacytometer and then treated with an Fc blocking buffer containing anti-Cd16/32 (BD Biosciences, 1:500) for 20 minutes at 4 degrees Celsius before a 30 minute incubation with antibody master mixes for the myeloid or lymphoid panels at 4 degrees Celsius ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with FACS staining buffer (PBS supplemented with 2% fetal bovine serum and 1 mM EDTA) and incubated with Fc receptor blocker purified rat anti-mouse anti-CD16/CD32 (BD Biosciences, 553142) for 20 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... plated at a density of 1 X 106 cells/well in a Matrigel-coated 6 well plates (BD, 354277; Fisher Scientific, 140675). For the first 24 hours after passaging ...
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bioRxiv - Bioengineering 2024Quote: ... 3-4×105 B16F10-ZsGreen cells were implanted subcutaneously (s.c.) into the right flank of mice after shaving through a 25G x 1 ½ in needle (BD PrecisionGlide Needle #305127). For the growth control and survival study ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... Permeabilization was followed by blocking cells using 5% BSA in HBSS+ for 30 min and incubated with the primary antibody solution - Mouse anti-human CD144 (1:150; 555661, BD Biosciences, USA) for overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then stained at a 1:50 dilution with antibodies to immune cell targets as follows: CD3- FITC (BD Biosciences, 555332), CD8-PE (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and resuspended in flow buffer containing 1% FA to be analyzed using the LSR Fortessa (BD Bioscience, USA). Data were analyzed using FlowJo 10 software (FlowJo LLC ...
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bioRxiv - Cell Biology 2022Quote: ... Pelleted cells were incubated with anti-GFP-AF647 antibody (1:150, Alexa Fluor 647 Mouse Anti-GFP Clone 1A12-6-18, BD Biosciences 565197) or rabbit anti-ITGA5 (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... using a 23 G needle and a 1 ml syringe.Blood was collected into a serum separator tube (365967, BD, Franklin Lakes NJ), allowed to clot at room temperature for at least 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: The following antibodies were obtained commercially and used at the indicated dilutions for immunofluorescence: mouse monoclonal TOM20 (BD Biosciences #612278, 1:1000), TOMM20 (Abcam #ab186734 ...