Labshake search
Citations for Becton, Dickinson and Company :
6601 - 6650 of 7984 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed two times with 150 µL PBS (centrifuged 5 min at 400 × g at 4 °C) and resuspended in 50 µL Fc-block (1:50, Purified Rat Anti-Mouse CD16/CD32, BD, #553142) diluted in FACS buffer (1x PBS/2% FBS/2mM ...
-
bioRxiv - Cancer Biology 2023Quote: ... per well of U-bottom plate and coincubated for 4h at 37°C in the presence of NH4Cl and 1 µl Golgi PLUG (BD Biosciences). After that time cells were washed three times with 200 µl PBS and centrifuged (5 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... CD8/CD4 T cells ratio and activation was assessed with BD Multitest CD3/CD8/CD45/CD4 (1:20; BD Biosciences, #342317) and APC-CD69 (1:100 ...
-
bioRxiv - Neuroscience 2022Quote: ... the cell suspension was then incubated on two PDGFRα plates coated with rat anti-PDGFRα IgG (BD Biosciences, 558774; 1:500). The adherent cells were washed with PBS to remove all antigen-negative nonadherent cells ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were fixed with IC fix (eBioscience88-8824-00) for 30 minutes followed by incubation with CD74 FITC 1:100 (BD 561941) in permeabilization buffer (eBioscience88-8824-00 ...
-
bioRxiv - Cell Biology 2022Quote: Freshly dissected LW whole mount preparations from 6 wild-type and 4 Lrp2-/267 mice were incubated with rat anti-CD24 conjugated with Phycoerythrin (PE; 1:100; BD Pharmingen) in Neurobasal medium (Gibco ...
-
bioRxiv - Cell Biology 2022Quote: For 3T3 NIH fibroblasts cells infected with C/EBPα-ER-IRES-hCD4 (WT and T35A) were incubated with 0.1µg per 1 million cells of both Fc block and hCD4-Biotin antibody (BD Pharmingen, Cat#555347) for 10 and 20 minutes respectively ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then washed twice with 1× PBS and flow cytometry analysis was performed on a FACS Canto II flow cytometer (BD Biosciences); data analysis was carried out using FlowJo software (BD Biosciences).
-
bioRxiv - Immunology 2022Quote: ... cultured splenocytes were resuspended in 40 μL FACS buffer containing fluorochrome-conjugated antibodies and incubated 1 hr at 4 °C followed by cell fixation using Cytofix/Cytoperm buffer (BD Bioscience) for 20 min at 4 °C ...
-
bioRxiv - Immunology 2022Quote: ... was added and 0.6-1×106 lymphocyte-gated events were immediately acquired on an LSRII SORP (BD, San Diego, CA, USA).
-
bioRxiv - Immunology 2022Quote: ... ELISAs were performed using paired rat anti-mouse IgE antibodies (capture with 1 μg/mL purified R35-72, BD Biosciences, detect with biotinylated R35-118, BD Biosciences) and streptavidin-HRP (BD Biosciences) ...
-
bioRxiv - Immunology 2022Quote: ... and others were fixed using PBS containing 1% paraformaldehyde (Labsynth, Diadema, SP, Brazil) and acquired in FACS Canto II (BD Biosciences). Analyses were performed in FlowJo software (Becton Dickinson and Company).
-
bioRxiv - Microbiology 2022Quote: ... xanthus cells were grown in liquid CYE medium (10 mM MOPS pH 7.6, 1% (w/v) Bacto™ casitone (BD Biosciences), 0.5% yeast extract and 8 mM MgSO4 ...
-
bioRxiv - Immunology 2022Quote: ... Biotinylated antibody bound to RBD in the presence of competitor was stained with Streptavidin-PE at a 1:1000 dilution (BD Biosciences) and analysed on a 200 Bioplex instruments ...
-
bioRxiv - Neuroscience 2022Quote: ... The resulting pellet was resuspended in 1 ml of complete neurosphere medium containing epidermal growth factor (EGF, 20 ng/ml; receptor grade, BD Biosciences) and basic fibroblast growth factor (bFGF ...
-
bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD8 APC (1:100, catalog no. 553035; BD Biosciences); rat anti-CD45 PE (1:100 ...
-
bioRxiv - Immunology 2022Quote: ... cells were stained for p-STAT1 (clone 4alpha) or p-STAT3 (clone 4P/STAT3) for 1 hour (all from BD Biosciences) and were acquired on FACS LSRFortessa flow cytometer (BD Biosciences).
-
bioRxiv - Immunology 2022Quote: ... Data acquisition and analysis: 0.5-1×106 events were collected on a BD LSR Fortessa X-50 flow cytometer (BD Immunocytometry Systems). Electronic compensation was performed with antibody capture beads (BD Biosciences) ...
-
bioRxiv - Immunology 2022Quote: ... GFP expression in peripheral blood B and T cells was screened using anti-CD19-PE (BD, clone 1D3, dilution 1/100), anti-CD3e-PerCP-Cy5.5 (eBioscience ...
-
bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, for the RBD ELISA, and BD OptEIA Substrate Solution ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in staining buffer consisting of 50% PBS containing 1% bovine serum albumin (BSA) and 50% Brilliant Stain Buffer (BD Biosciences) and incubated for 20 minutes at 4°C in the following antibodies ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... then the resulting 350 μL volume was drawn twice into a 1 mL luer slip tip tuberculin syringe (BD Catalog # 309659) fitted with a 27G-½” needle ...
-
bioRxiv - Developmental Biology 2023Quote: ... or (ii) PE-Annexin V conjugated antibody (1:33 dilution in TBST) and 7-AAD as per kit instructions (BD Pharmingen). Samples were analysed by flow cytometry within 30 min.
-
bioRxiv - Developmental Biology 2023Quote: ... and then resuspended in 100 μL Annexin V binding buffer with either (i) FITC-Annexin V conjugated antibody (1:33 dilution in TBST) and BD propidium iodide staining solution (BD Pharmingen) or (ii ...
-
bioRxiv - Immunology 2023Quote: ... Single cell suspensions of ex vivo organs were co-cultured with DCs in complete RMPI at a ratio of approximately 10 T cells to 1 DC in the presence of Golgi Plug (BD Bioscience). Co-cultures were incubated at 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... HC-04 cells were washed and fixed with 1% PFA in PBS before quantifying dextran-positive cells by flow cytometry (LSR II instrument, BD Biosciences). For data analysis using FlowJo V.10.0.8 (Tree Star) ...
-
bioRxiv - Cancer Biology 2023Quote: ... mice bone marrow mononuclear cells were first stained with lineage (Lin) cocktail containing biotinylated antibodies against Ly-6G/Ly-6C (Gr-1) Biotin RB6-8C5 (BD Bioscience) (1:100) ...
-
bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were incubated with Fc block (anti-mouse CD16/CD32, 1 μg for 1x106 cells, BD Biosciences, Cat# 553142) at 4°C for 30 min ...
-
bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
-
bioRxiv - Immunology 2024Quote: ... CFSE-labelled cells were boosted with HIV-1-Gag or vaccine-specific peptide pools in the presence of Brefeldin A solution (BD, Biosciences) and Monensin solution (BD Biosciences) ...
-
bioRxiv - Cell Biology 2024Quote: ... ESLAM HSCs defined as CD45+EPCR+CD48-CD150+ as previously described,1 were isolated using a Becton Dickinson Influx sorter (BD Biosciences). Cells were sorted directly into culture dishes containing media or into Eppendorfs containing FACS buffer.
-
bioRxiv - Microbiology 2024Quote: ... and 1 µg/mL menadione (United States Pharmacopeia Convention) and incubated statically overnight at 37 °C in an anoxic atmosphere (BD GasPak). S ...
-
bioRxiv - Immunology 2024Quote: Cells were labelled with unique sample identifier tags (Sample Tag 1-12) using the BD Human Single-Cell Sample Multiplexing Kit (633781/BD Bioscience) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... blood was collected via intracardiac puncture using a 1 mL 25-gauge Tuberculin syringe and transferred to EDTA-coated microtainer tubes (BD, #365974), inverted eight-to-ten times ...
-
bioRxiv - Immunology 2024Quote: ... were cultured in flat-bottom 96-well plates pre-coated with 1 µg/mL anti-CD3 antibody (clone OKT3; ATCC, Manassas, VA) in supplemented RPMI media containing 1 µg/mL soluble anti-CD28 antibody (BD Pharmingen) with or without 50% volume of activated macrophage supernatant ...
-
bioRxiv - Immunology 2024Quote: ... Stimulation was performed for 20 h at 37°C in the presence of 1 μL/mL GolgiPlug™ (BD Biosciences, 555029). For the unstimulated condition ...
-
bioRxiv - Immunology 2024Quote: ... Tissues were then harvested and fixed for 24 hours in 1ml fixative buffer (BD Cytofix/Cytoperm prepared 1:4 buffered to PBS; BD Biosciences) at 4°C on a slow shaker ...
-
bioRxiv - Immunology 2024Quote: ... the cells were washed with the kit washing buffer and stained with the intracellular mix containing the following anti-human antibodies: Ki67 (BUV395, 1/160, BD Biosciences) and Foxp3 (AF488 ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were diluted 1:25 in YES media into mid-log phase and analyzed by flow cytometry on an LSR Fortessa X50 (BD Biosciences). Fluorescence compensation ...
-
bioRxiv - Microbiology 2024Quote: ... of pressurized culture were taken using a 1 mL Injekt-F syringe (Braun) fitted with a 23-gauge sterile needle (BD Microlance) by puncturing the rubber fitting ...
-
bioRxiv - Immunology 2024Quote: ... and then stained with the following antibodies to validate B cell depletion: CD45 BUV395 (BD Biosciences clone 30-F11; 1:200), CD19 BV737 (BD clone 1D3 ...
-
bioRxiv - Immunology 2024Quote: ... cells were stained with a combination of fluorochrome-conjugated antibodies as specified in supplementary Table 1 (sTable1) and sorted (FACSAria III, BD Biosciences), according to the expression of following specific surface markers combination ...
-
bioRxiv - Immunology 2024Quote: ... cells were washed in Fluorescence Activated Cell Sorting (FACS) buffer (1% BSA, DPBS) and then incubated with Fc receptor-blocking antiCD16/CD32 antibody (BD Pharmingen) in FACS buffer for 15 minutes at 4⁰C before staining with the appropriate fluorochrome-conjugated antibodies (Table 2 ...
-
bioRxiv - Immunology 2024Quote: ... total asthmatic lung cells (0.5 million) were cultured in flat bottom 96-well plates coated with 1 µg/ml of anti-CD3 (145-2C11, BD Biosciences) and 1 µg/ml anti-CD28 (37.51 ...
-
bioRxiv - Immunology 2024Quote: ... 10 μg/ml of ESAT-6 and CFP-10 full proteins (BEI Resources) + anti-human CD28/CD49d antibodies at 1 μg/ml (BD Bioscience). Samples were incubated at 37°C and 5 μg/ml of Brefeldin A (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... unsorted, sorted, and depleted fractions, were labelled with mouse anti-ASGPR1-PE (1:20 dilution, to detect porcine hepatocytes) (BD Pharmigen), rat anti-OC2-2F8 ...
-
bioRxiv - Neuroscience 2024Quote: ... The organs of corti were then dissected from the otic capsule and co-stained with anti-Ctbp2 antibodies (1:800; 612044, BD Transduction) and anti-myosin VI antibodies (1:400 ...
-
bioRxiv - Neuroscience 2024Quote: ... Additional samples were stained and analyzed as above using the following modifications: the samples were blocked with FcR block (1:200 dilution; 553141, BD Biosciences) for 10 min at 4°C and incubated with anti-mouse CD11b-PE (1:50 dilution ...
-
bioRxiv - Immunology 2024Quote: Activation of signaling pathways downstream of pre-TCR was analyzed by western blotting of cells incubated with 10 µg/ml of anti-CD3ε (UCHT-1; BD Biosciences) or anti-pTα (K5G3 ...
-
bioRxiv - Immunology 2024Quote: ... positive for wild type heterologous SOSIP probes and negative for V2 apex epitope mutant probes were sorted at 1 cell per well into 96-well plates on a BD FACSAria II machine using BD FACSDiva software (BD Biosciences). RNA extraction ...