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Citations for Becton, Dickinson and Company :
601 - 650 of 1839 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Enrichment of surface expression in PE-selected cells was confirmed by flow cytometry using a BD FACS LSR II cytometer (BD Biosciences) and analyzed with FlowJo software (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... was used as a secondary antibody. The surface expression levels of S proteins (Fig. 4A) were measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (FlowJo LLC) ...
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bioRxiv - Neuroscience 2023Quote: Expression of the different fluorescent proteins (ORFs) of the stable GEFRI2 line were measured by High Throughput LSR Fortessa (BD Biosciences) fluorescence-activated cell sorter (FACS) ...
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bioRxiv - Cell Biology 2023Quote: ... Infected cells were then selected with puromycin and blasticidin and cells with moderate expression levels were enriched by a FACSAria II cell sorter (BD Bioscience).
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bioRxiv - Immunology 2022Quote: ... GFP expression in peripheral blood B and T cells was screened using anti-CD19-PE (BD, clone 1D3, dilution 1/100), anti-CD3e-PerCP-Cy5.5 (eBioscience ...
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bioRxiv - Immunology 2023Quote: Cells were surface stained with fluorochrome-conjugated mAbs to examine the expression of the phenotypic markers CD3 (clone UCHT1, BD Pharmingen), CD4 (RPA-T4 ...
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bioRxiv - Cancer Biology 2024Quote: ... BM was analyzed for huCD45 (BDPharmingen, Cat#555485) and huCD33 (BDPharmingen, Cat#555450) expression by flow cytometry using a BD LSRFortessa (BD Biosciences). For staining ...
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bioRxiv - Cancer Biology 2023Quote: ... the MSCs and ALL cells were removed from the plate by trypsinisation and sorted on expression of CD90 using anti-CD90 FITC or anti-CD90 APC (BD Biosciences) and lack of expression of CD19 using anti CD19 APC or CD19 BV605 (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... mock or INHBA overexpression CT26 cells were directly analyzed for PD-L1 expression (BV650 Rat Anti-Mouse CD274; 740614, BD Bioscience) by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... Luc-DOHH2 cells were isolated using a cell sorter based on their co-expression of GFP (FACS Aria II, BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... Fc blocking and fluorescent staining were performed as previously described and samples sorted into pure cell populations based on cell surface marker expression using a FACS Aria Fusion (BD Biosciences).
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bioRxiv - Cancer Biology 2024Quote: ... The media was replaced the next morning and the cells were trypsinized 48-hours post-transfection for analysis of GFP expression by flow cytometry (BD Biosciences).
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bioRxiv - Cell Biology 2024Quote: Analysis of Spry4:H2B-Venus reporter expression in live or fixed cells was performed on a LSRII flow cytometer (BD Biosciences). Cell sorting and analysis of GATA6-mCherry expression was carried out using a FACS Aria Fusion (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... The maturity of differentiated HL60 cell was probed by CD11b expression on flow cytometry using anti-CD11b-FITC antibody (BD Biosciences). Differentiated HL60 cells were harvested and stimulated with 10 µg/mL of resistin for 16 hours ...
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bioRxiv - Biophysics 2020Quote: ... mKO2 and EGFP in correlation with the expression levels of GV genes using a BD FACS Aria Ⅲ cell sorter (BD Bioscience). Spectral overlaps of each fluorescence were calibrated using the cells expressing each single fluorescent protein gene ...
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bioRxiv - Microbiology 2020Quote: ... The sorted GFP− cells were stimulated with 10 ng/mL TNF-α for 6 h and then GFP expression levels were analyzed using BD FACSVerse (BD Biosciences). The level of gag expression after 18-h stimulation with 10 ng/mL TNF-α was analyzed by reverse-transcription (RT)-digital droplet PCR (ddPCR) ...
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bioRxiv - Biophysics 2020Quote: ... We selected transfected cells with blasticidin for OsTIR1 integration and sorted them via the GFP signal for tdMCP-tdGFP expression using FACS (BD FACSAria II).
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bioRxiv - Immunology 2022Quote: ... Marker expression was analyzed in a Guava 8HT using Guava ExpressPro software (Luminex, USA) or BD LSRII using FACSDiva software (BD Biosciences, USA) and analyzed with FlowJo software (v10 ...
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bioRxiv - Molecular Biology 2020Quote: ... Then the syndecan-4 expression on HUVECs was measured by flow cytometry with a BD Biosciences LSR II (BD Biosciences, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Transduced cells were then expanded further in culture and FACS sorted for mStrawberry expression (BD-FACS Aria, BD Bioscience, Franklin Lakes, NJ).
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bioRxiv - Immunology 2020Quote: ... Human cytokines expression (hTNFα and hIFNγ) in human T cells were analyzed using BD LSRFortessa™ cell analyzer - flow cytometer (BD Biosciences) and the data were analyzed using FlowJo software (Dako).
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bioRxiv - Immunology 2019Quote: ... Introduced adaptor expression was tested via expression of EmGFP encoded on the adaptor lentivector (BD FACSCalibur™, 488 nm laser, FL1 - 530/30 band-pass filter, BD Biosciences). IG4 TCRβ and CD8α expression was analysed using anti-TCR Vβ 13.1 antibody FITC (H131 ...
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bioRxiv - Microbiology 2021Quote: ... Stained PBMCs were assessed for the expression patterns of the above immune cell markers by flow cytometry (FACS Aria II, BD Biosciences, USA). For each cell type ...
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bioRxiv - Immunology 2021Quote: ... NKT and CD4 T cells were sorted on the basis of TCR-β and PBS-57 loaded CD1d tetramer expression using a FACS Aria II (BD Biosciences). To study activated NKT cells ...
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bioRxiv - Immunology 2022Quote: ... cDNA was amplified—10 cycles for resting cells and 9 cycles for in vitro-stimulated cells using the BD Rhapsody™ T Cell Expression Panel (BD Biosciences) combined with a supplementary panel developed in-house targeting a total of 1,066 different genes (table S4) ...
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bioRxiv - Cell Biology 2023Quote: ... T cells with residual target protein expression were depleted by antibody staining and bead pull-down or cell sorting (BD Aria III). T cells were re-suspended in MACS buffer (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cell lines were evaluated for expression of B7-H3 using B7-H3 antibody (clone 7-517, BD or clone FM276, Miltenyi).
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bioRxiv - Immunology 2023Quote: ... A target primer panel was constructed by combining a commercial predesigned Human T Cell Expression Primer Panel (BD Biosciences, 259 target primers) with our custom-designed primer panel (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... 25 μL medium containing 1 × 106 luciferase-expressing MCF10AER/vSrc cells (control or 4-OHT transformed, with or without NLS-catalase expression) were mixed with 75 μL Matrigel matrix (BD Bioscience, #356237) containing 10 μM 4-OHT and injected into the inguinal mammary fat pad of the female mice at 6 to 8 weeks of age ...
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bioRxiv - Cancer Biology 2024Quote: ... Positively transduced cells were selected with 2 µg/ml puromycin followed by a sterile sort for high GFP and mCherry expression (BD FACSAria II). Expression was maintained with 1 µg/ml puromycin in all experiments except where immune cells were needed.
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bioRxiv - Developmental Biology 2023Quote: ... lung mesenchymal cells from Tagln-YFP/Cspg4-DsRed reporter mice were further sorted into perivascular SMCs and non-perivascular SMCs based on their fluorescent reporter expression using FACS (BD, FACSAria I). The cells were then plated at low density (∼104/100-mm dish ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines created with fluorescently tagged proteins were sorted and normalized for expression using the UC Berkeley Flow Cytometry Facility (BD Bioscience Influx Sorter). Cell lines were confirmed with confocal imaging and immunoblot.
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bioRxiv - Microbiology 2020Quote: ... the Spike protein expression level was quantified as the mean of the fluorescent intensity (MFI) in the PE channel using flow cytometry (BD Accuri™ C6).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were allowed to recover for 2 days in 6-well plates filled with 4 ml medium per well before being FACS sorted for mCherry-expression on a BD FACS Aria III (BD Biosciences, Heidelberg, Germany). For subsequent limiting dilution cloning ...
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bioRxiv - Microbiology 2021Quote: ... Total gB expression was determined by using the same staining protocol except cells were permeabilized using Cytofix/Cytoperm (BD Biosciences, San Jose, CA) before adding the primary antibody and during the staining procedure ...
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bioRxiv - Cancer Biology 2020Quote: ... The eGFP-ffluc-transduced PDX6 cells were selectively isolated based on GFP expression via FACSAria cell sorter (BD Biosciences, San Jose, CA, USA). PDX6 cells were injected into the right ovarian bursa of nude mice with Matrigel (354248 ...
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bioRxiv - Microbiology 2021Quote: ... Off-line samples were taken from the reactors each 5-10 h and the analysis of the GFP expression levels was performed with a BD Accuri C6 (BD Biosciences, CA, USA) based on FL1-A channel (excitation 488 nm ...
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bioRxiv - Bioengineering 2023Quote: ... For strains with simultaneous yeGFP and miRFP expression the fluorescence compensation wizard was used in the FlowJo software (BD Life Sciences, version 10.8.1).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell population used for further studies consisted of more than 85% monocytes according to forward and side scatter properties and CD14 surface expression (BD FACS Calibur flow cytometer, BD Biosciences, Heidelberg, Germany). Experiments were approved by the ethical commission of the Friedrich-Schiller-University Jena.
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bioRxiv - Microbiology 2019Quote: ... following which they were trypsinized and assessed for GFP expression via fluorescent activated cell sorting using the Accuri C6 (BD Biosciences, San Jose, CA). Analysis was performed using FlowJo Macintosh v10.1 (FlowJo ...
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bioRxiv - Cell Biology 2019Quote: ... hMSCs were sorted based on their level of expression of CD146 or their size (FSC and SSC) using a FACSAria III (BD Bioscience, San Jose, CA).
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bioRxiv - Immunology 2021Quote: ... Proliferation of CPD-labeled T cells measured by dilution of the CPD signal and EGFP expression were analyzed using a FACSVerse system (BD Biosciences, Franklin Lakes, NJ) and FlowJo software (Tree Star ...
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bioRxiv - Immunology 2020Quote: ... The 3T3 cells were FACS sorted four times for expression of CD40L using an anti-CD40L antibody (clone TRAP1, BD Bioscience, San Jose, California). This resulted in a stable CD40L-expressing cell line that was cultivated in G418 containing selection media to maintain expression ...
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bioRxiv - Biochemistry 2024Quote: ... for cell surface expression of CCR6 by determining the mean fluorescence intensity (MFI) of BV-421 using FlowJo™ Software (Becton, Dickinson and Company).
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bioRxiv - Immunology 2020Quote: ... of HLA-A2 expression on the surface of RMA-S-HHD cells was measured by flow cytometry (FACSCanto™ II, BD Biosciences, Franklin Lakes, NJ), and standardized as the percent cell surface expression by the following formula ...
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bioRxiv - Immunology 2020Quote: ... Jurkat cells were maintained in culture for 1 week using enhanced RPMI and screened for TCR expression (Jing et al., 2016) using an anti-mouse TCR β chain (mTCRBC) APC antibody (BD Biosciences, San Jose, CA) and tetramer staining as described above.
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bioRxiv - Immunology 2021Quote: ... Amplification kit and BD Single-Cell Multiplexing kit (BD Biosciences). Size-distribution (Quality ...
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bioRxiv - Microbiology 2021Quote: ... a BrdU assay kit (APC BrdU flow kit, BD Pharmingen) was used as directed by the manufacturer’s instruction ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... Viral production titers were assessed on HT1080 cells with serial dilutions and scored for GFP expression by flow cytometry analysis on a BD FACSVerse (BD Biosciences, Le Pont de Claix, France).
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bioRxiv - Cancer Biology 2019Quote: ... Gates were set to sort cell populations having low 10% and high 10% expression of ITGB4 using the FACSAria™ Fusion (BD Biosciences, San Jose, CA, USA). Sorted cells were immediately cultured in 12-well plates and treated with cisplatin (1 μM ...