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Citations for Becton, Dickinson and Company :
551 - 600 of 1839 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and fluorophore expression was measured in a minimum of 10,000 events per sample with a BD LSR II Fortessa 4L (BD Biosciences). HIV-1- and/or Ad-positive were analyzed with FlowJo software v10.0.8.
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bioRxiv - Molecular Biology 2023Quote: ... two-color analysis of murine or human platelet activation was performed using fluorophore-labeled antibodies for P-selectin expression (Emfret Analytics, Wug.E9-FITC, #M130-1, and BD Biosciences ...
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bioRxiv - Synthetic Biology 2024Quote: Cell lines were bulk sorted for high expression using the UCSF Laboratory for Cell Analysis Core Facility FACSAriaII (BD Biosciences). Cells were assessed for BFP (405nm excitation ...
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bioRxiv - Synthetic Biology 2024Quote: Cell lines were bulk sorted for high expression using the UCSF Laboratory for Cell Analysis Core Facility FACSAriaII (BD Biosciences) or a Sony SH800.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell population used for further studies consisted of more than 85% monocytes according to forward and side scatter properties and CD14 surface expression (BD FACS Calibur flow cytometer ...
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bioRxiv - Cell Biology 2020Quote: ... and 18h later the cells were collected to measure expression of GFP and IFP (for transfection efficiency normalization) by flow cytometry in a FACS LSRII Instrument (BD Biosciences). The data collected was analyzed using FlowJo software as described (79) ...
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bioRxiv - Immunology 2021Quote: ... then sorted GFP+ cells were expanded and assessed for CD47 expression by staining with BV786-conjugated anti-hCD47 mAb B6H12 (BD Bioscience) and PE-conjugated anti-hCD47 mAb CC2C6 (Biolegend) ...
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bioRxiv - Molecular Biology 2021Quote: Surface marker expression of MSC clones was carried out by flow cytometry using the BD FACS Canto II flow cytometer (BD Biosciences) using antibodies against CD3 ...
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. Surface expression level of S proteins (Extended Data Fig. 6a) was measured using FACS Canto II (BD Biosciences) and the data were analysed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Genetics 2022Quote: ... Cells were incubated at 37°C with 5% CO2 for 24 h and then sorted for GFP+ expression using fluorescent activated cell sorting (FACS) on a FACSAria II (BD Bioscience). The GFP+ pool (bulk ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were harvested by trypsinisation at 16-20 hrs post infection and evaluated for GFP expression on a BD LSRFortessa cell analyser (BD Biosciences). The results were analysed using FlowJo software (FlowJo LLC ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Cells were allowed to recover for 2 days in 6-well plates filled with 4 ml medium per well before being FACS sorted for mCherry-expression on a BD FACS Aria III (BD Biosciences). For subsequent limiting dilution cloning ...
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bioRxiv - Immunology 2021Quote: ... the killing capacity of NK cells was tested when they coculture with senescent / young or modulated for ST8SIA1 expression MRC5.NK cells are added to the culture for 4 h in presence of monensin and brefeldin (BD Biosciences) at the effector/target ratio of 1:1 ...
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bioRxiv - Cancer Biology 2022Quote: ... BM cells were surface labeled for B220 and sIgµ expression and B220+sIgµ- cells were sorted using a FACS Aria III (BD Bioscience). Sorting efficiency was routinely above 95 % ...
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bioRxiv - Cell Biology 2022Quote: ... filtered with a 3 μm filter and sorted for YFP expression into a 96 well plate (5 and 10 events; 48 wells each) (FACSARIA III, BD Biosciences). Individual plaques were screened by PCR and all modified loci were sequenced to confirm correct modification ...
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bioRxiv - Immunology 2022Quote: PBS-washed cells were PFA-fixed and immunostained for individual surface protein expression using the following antibodies: Anti-CD3-FITC (#561807; BD Biosciences), anti-CD4-APC (#555349 ...
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bioRxiv - Biochemistry 2022Quote: ... TCR expression on transfected Jurkat cells was assessed by staining with phycoerythrin (PE)-conjugated mouse anti-human CD3 (BD Pharmingen 555333); TCR expression was unaltered by Csk overexpression.
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bioRxiv - Microbiology 2021Quote: ... or high (rhCD4HIGH) levels of rhCD4 expression were obtained by sorting the cells on a FACSAria II cell sorter (BD Biosciences) using an allophycocyanin (APC)-conjugated CD4 monoclonal antibody (BD Biosciences catalog no ...
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bioRxiv - Immunology 2021Quote: ... sequenced reads were aligned to the hg38 human transcriptome and the expression of transcripts in each cell was quantified via the standard Rhapsody analysis pipeline (BD Biosciences) on Seven Bridges (https://www.sevenbridges.com) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were suspended in 200 µl of 1% FBS and cells were sorted by GFP expression using FACSCVantage SE (BD Biosciences). The cell cycle profiles of GFP-positive and GFP-negative cells were determined by Hoescht flourescence using a FACSCalibur flow cytometer (Becton Dickinson) ...
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bioRxiv - Immunology 2021Quote: ... After incubation the cells were washed twice with FACS wash buffer and then acquired for differential expression analysis using LSRII fortessa flow cytometer (BD Biosciences). The acquired data was analyzed using FlowJo-X software (Treestar) ...
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bioRxiv - Immunology 2020Quote: ... Interleukin-6 (IL-6) expression was measured by ELISA in SF-supernatants according to the manufacturer’s instructions (BD Biosciences, Oxford, UK).
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bioRxiv - Cancer Biology 2020Quote: ... T cells were analyzed for proliferation at day 5 via Cell trace dye (eBioscience) and inhibitory receptor expression (LAG3 and PD1) on a Fortessa II (BD Bioscience) and data analyses were performed via FlowJo (Tree Star).
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bioRxiv - Immunology 2021Quote: ... of HLA-A*24:02 expression on the surface of RMA-S-HHD-A24 cells was measured by flow cytometry (FACSCanto II, BD Biosciences), and standardized as the percent cell surface expression by the following formula ...
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bioRxiv - Neuroscience 2020Quote: ... the cells were analyzed and sorted by FACS based on GFP expression using a BD FACSAria III Cell Sorter (BD Biosciences).
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... collected in PBS and examined for mCherry expression using a BD FACSAria Cell Sorting System with BD FACSDiva Software (BD Biosciences). Non-fluorescent cells were negatively selected and the remaining gated cells were sorted based on the presence of mCherry fluorescent protein ...
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bioRxiv - Developmental Biology 2022Quote: ... and cells with high expression of both cherry and BFP were isolated with fluorescence activated cell sorting with a FACSAria cell sorter (BD Biosciences). Clones grown from sorted cells were expanded and DNA samples from individual clones were extracted with Lucigen quick DNA buffer (68 °C for 15 min followed by 98 °C for 2 min) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were dissociated with Accutase and mCherry expression in the singlet cell population was analyzed using a FACSymphony A5 flow cytometer (BD Biosciences).
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bioRxiv - Neuroscience 2022Quote: ... and mCherry expression in the EGFP positive singlet cell population was analyzed using the BD LSR II flow cytometer (BD Biosciences).TP53-deficient KOLF2 cells (W Skarnes ...
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bioRxiv - Cell Biology 2019Quote: ... Single cell clones were expanded and analyzed for stable expression of EPO-eGFP and A1AT-mCherry using a LSR Fortessa flow cytometer (BD Bioscience).
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bioRxiv - Microbiology 2020Quote: ... A minimum of 5 000 – 10 000 cells was analyzed per sample for YFP expression on a LSRII flow cytometer (BD Biosciences). Data was analyzed using Flowing Software (Version 2.5).
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bioRxiv - Immunology 2020Quote: ... and 2 mM KH2PO4)-FBS 5% for 15 min and then stained for surface expression with fluorophore-marked antibodies against SLAMF1 (BD, 559572), CD31 (BD ...
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bioRxiv - Immunology 2020Quote: ... cells were harvested and either resorted based on viability for Fluidigm gene expression analysis or were immediately fixed for 20 min at room temperature (100μL, BD cytofix/cytoperm) for Ki67/DAPI analysis or puromycin incorporation assays ...
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bioRxiv - Immunology 2021Quote: ... CD8+EGFP-Tconv and CD8+EGFP+ Treg cells in blood and spleen as well as the expression levels of Stat-5 phosphorylation (clone pY694, BD Biosciences) and CD25 (clone OX39 ...
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bioRxiv - Immunology 2020Quote: ... FoxP3-GFP and ErbB2-CAR transgenic mice were then sorted on the basis of a CD4+CD8-CD62L+CD44-GFP-CD25- expression profile on a FACSAria flow cytometer (BD Biosciences). Thymic Tregs (tTregs ...
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bioRxiv - Immunology 2020Quote: ... Fc blocking and fluorescent staining was performed as previously described and samples sorted into pure cell populations based on cell surface marker expression using a FACS Fusion (BD Biosciences). For data where peritoneal populations are expressed as cells/ μl ...
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bioRxiv - Immunology 2021Quote: ... the donor cell reconstitution was confirmed by differential expression of congenic markers in peripheral blood using the BD FACSCanto II (BD Biosciences).
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bioRxiv - Genomics 2021Quote: ... cells expressing GFP (expression construct marker) and BFP (sgRNA marker) were isolated by fluorescence-activated cell sorting on a FACSAria Fusion (BD Biosciences). On day 6 ...
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bioRxiv - Genomics 2021Quote: ... Purity of the cell cultures was determined by measuring the expression of the cardiomyocyte-specific marker cardiac troponin T (564767, BD Biosciences) by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... all from eBioscience) and assessed for the expression of these markers in addition to eGFP by flow cytometry using an LSRII (BD Biosciences). Cells were also incubated with mAb against α4β7 (clone DATK32 ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h and analyzed for fluorescence expression using a flow cytometer (BD FAC AriaIII sorter; BD biosciences, New Jersey, USA). A viral titration curve was constructed using viral dilutions on the X-axis and percentage EGFP+ve cells on the Y-axis ...
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bioRxiv - Immunology 2022Quote: ... DCs were analyzed for expression of maturation markers on the cell surface by flow cytometry using an LSR II (BD Biosciences) and FlowJo v10.1 software (University of Washington Cell Analysis Facility).
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bioRxiv - Cancer Biology 2022Quote: ... CD137 expression on CD8+ T cells was assessed after an overnight co-culture with an anti-CD137 PE antibody (BD Biosciences).
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bioRxiv - Developmental Biology 2022Quote: ... Primordial germ cells were purified based on the expression of Zombie Red−/SSEA-1+/CD61+ using BD FACS Aria II Cell Sorter (BD Bioscience). Somatic gonad cells were purified based on the expression of Zombie Red−/SSEA-1−/CD61 ...
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bioRxiv - Immunology 2023Quote: ... Foxp3-GFP were then sorted on the basis of a CD4+CD8-CD62L+CD44-GFP-CD25- expression profile on a FACSAria flow cytometer (BD Biosciences). T cell activation was performed using plate-bound α-CD3 (clone 145-2C11 ...
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bioRxiv - Biophysics 2023Quote: ... Enrichment of surface expression in PE-selected cells was confirmed by flow cytometry using a BD FACS LSR II cytometer (BD Biosciences) and analyzed with FlowJo software (BD Biosciences).
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. The surface expression level of S proteins (Fig. 3d) was measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... P33681) without the leader sequence was PCR-amplified and cloned into the pAcGP67 of the BaculoGold Baculovirus Expression System (BD Biosciences). This vector was modified so that all proteins featured a C-terminal H12-tag ...
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bioRxiv - Immunology 2022Quote: ... The cells were fixed in 2% paraformaldehyde and GFP expression measured and quantitated using a FACS Canto II flow cytometer and FlowJo software (BD Biosciences). IC50 values were calculated using Prism software (GraphPad/Dotmatics).
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. The surface expression level of S proteins (Fig. 3d) was measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...