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Citations for Becton, Dickinson and Company :
6351 - 6400 of 6484 citations for Mouse Bcl 2 Modifying Factor BMF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: Exponentially growing cells in six-well plates were treated with 3 μM BAY-876 or DMSO for 24h before cell cycle analysis using allophycocyanin (APC) BrdU Flow kit (BD Pharmingen). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: Cells were double stained with propidium iodide (PI) and Annexin V by using FITC Annexin V apoptosis detection kit (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... were sorted directly into four 96 well plates « Precise WTA Single Cell Encoding Plate » (BDTM Precise WTA Single Cell Kit, BD Genomics) (1 cell/well ...
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bioRxiv - Immunology 2020Quote: ... Total CD4+ T cells were isolated by negative magnetic selection using a CD4 T cell isolation kit (Stemcell) and CD4+CD25hiCD127lo-neg Treg cells were sorted on a FACS Aria (BD Biosciences). Treg cells were cultured in RPMI 1640 medium supplemented with 5% Human serum ...
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bioRxiv - Cell Biology 2019Quote: ... Phenotypic characterization of each lot was done by flow cytometry using the BD StemflowTM hMSC Analysis kit and the endothelial cell marker CD31 (all reagents from BD Bioscience) and showed that the 2 lots of hUC-MSC met the ISCT phenotypic criteria for MSCs (>95% positive for CD73 ...
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bioRxiv - Genetics 2021Quote: ... at least 2x106 iPSC-PPC cells were fixed and permeabilized using the Fixation/Permeabilization Solution Kit with BD GolgiStopTM (BD Biosciences) following manufacturer recommendations ...
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bioRxiv - Cell Biology 2020Quote: The following monoclonal antibodies (Abs) were used for flow cytometry and cell sorting: anti-c-Kit (2B8, BD Biosciences, 1:100), -Sca-1 (E13-161.7 ...
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bioRxiv - Pathology 2021Quote: ... CD11b) and Sca1+/c-Kit+ cells in lineage negative cells determined via flow cytometry on a BD LSRII instrument (BD Biosciences). Data was analyzed on FlowJo software (Treestar) ...
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bioRxiv - Immunology 2021Quote: ... 4 and 6 hours and cytokines detected by intracellular cytokine staining (see Table S2 for antibodies list) using Fixation/Permeabilization Solution Kit (BD Biosciences), unstimulated cells were used as a control.
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bioRxiv - Immunology 2020Quote: ... stimulation of single cell suspensions in the presence of GolgiPlug for 4 hours prior to intracellular staining using BD Cytofix/Cytoperm kit (BD Biosciences). Viability was determined using Fixable Viability Dye eFluor 780 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... either cell supernatants or cell extracts were used for cytokine estimation with specific kits- TNFα (cat no: 88-7346-77, eBioscience) and IL1β (cat no: 557953, BD Biosciences) ELISA kits according to manufacturer’s protocol.
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bioRxiv - Microbiology 2019Quote: ... we diluted test cultures 100-fold and stained with thiazole orange and propidium iodide (BD Cell Viability Kit, product no. 349483). In parallel ...
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bioRxiv - Immunology 2020Quote: ... Spleen B cells were analyzed for BrdU incorporation (by anti-BrdU mAb staining) and DNA contents (by 7–AAD staining after cells were permeabilized) using the APC BrdU Flow kit® (BD) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and intracellular staining was performed after the fixation and permeabilization of the cells (intracellular staining kit from ThermoFisher or BD Biosciences). Analysis of cells collected from in vivo experiments were performed following Fc block (2.4G2 ...
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bioRxiv - Immunology 2020Quote: ... We only used iTreg cells for experiments if samples achieved at least 80% Foxp3 positive cells (00552300, Foxp3 Staining Kit, BD Bioscience).
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bioRxiv - Immunology 2021Quote: ... Spike -specific T cells were measured by surface staining followed by fixation and permeabilization using BD fixation/permeabilization solution kit (BD Biosciences,) and intracellular cytokine staining (table S1) ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated and immunolabeled with anti-CD4 and anti-Foxp3 antibodies and BrdU incorporation was analyzed by a BrdU Flow kit per the manufacturer’s protocol (BD Pharmingen, 552598) (15).
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bioRxiv - Genomics 2022Quote: ... Libraries were then prepared using the BD Rhapsody WTA Amplification Kit (#633801) following instructions of the mRNA WTA Library Preparation Protocol (BD Biosciences). For the MULTI-seq library first the BD protocol of Sample Tag Library Preparation was followed until purification of Sample Tag PCR1 product with the difference of adding the MULTI-seq primer (sequence according to McGinnis and Patterson et al.5 ...
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bioRxiv - Immunology 2022Quote: RFP+GFP+ FOXP3+ Tregs and RFP+GFP- exFOXP3 T cells isolated from spleen and CNS from EAE mice were stained with sample tag antibodies (Ms Single Cell Sampling Multiplexing Kit, BD Biosciences). Next ...
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bioRxiv - Immunology 2022Quote: ... and cells were fixed and permeabilized at room temperature for 15 min with Fixation/Permeabilization Solution Kit (BD Biosciences; cat. #554714). For each sample a minimum of 1.2×105 stopping gate events (live CD3+ T-cells ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: The distribution of cell populations throughout the cell cycle and the fraction of cells capable of incorporating BrdU were determined using the BrdU Flow Kit (BD Biosciences). After treatments cells were incubated with BrdU (10 ⎧M ...
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bioRxiv - Bioengineering 2024Quote: ... and the hemoglobin contents were quantified after 8 h by the colorimetric assay kit (absorbance: 550 nm; with BD Pharm Lyse). Each value was subtracted from the internal control without the anti-BSA antibody.
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bioRxiv - Neuroscience 2024Quote: The rate of apoptosis induced by microRNA-29a-5p mimics and inhibitors transfection in astrocytes after OGD/R was determined using Annexin V/PI kit (BD Biosciences). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Cell apoptosis was measured by co-staining cells with Annexin V and 7-AAD using the PE Annexin V Apoptosis Detection Kit I (BD Biosciences), according to manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... and CD45.2+ peripheral blood leukocytes were FACS-sorted and labeled using the BD Rhapsody Single-Cell Multiplexing CTT Kit (BD Biosciences, 626545). On average ...
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bioRxiv - Cell Biology 2023Quote: ... Post incubation cells were processed for apoptosis using flow cytometry according to the protocol described in FITC Annexin-V apoptosis detection kit (BD Pharmingen).
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bioRxiv - Genetics 2023Quote: ... 1.106 cells were incubated 20 min at 37°C in 100 μL solution BD cytofix/ cytoperm (BD PharmingenTM BrdU Flow Kit, 552598, BD Bioscience), washed with PBS and re-suspended in 700 µl of staining buffer (PBS with 3% SVF) ...
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bioRxiv - Immunology 2023Quote: ... and ionomycin (500 ng/mL) for 3 hrs at 37°C prior to surface and intracellular staining using the BD Cytofix/Cytoperm Kit (BD Biosciences). Intracellular transcription factor staining was performed using a Foxp3 staining kit (ThermoFisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... The final cDNA library was generated from double strand full length cDNA by random priming amplification with the BD Rhapsody cDNA Kit (633773, BD Biosciences) and the BD Rhapsody Targeted mRNA & AbSeq Amplification Kit (633774 ...
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bioRxiv - Cancer Biology 2023Quote: ... The staining procedures were performed by the use of a Cell Cycle and Apoptosis Analysis Kit (Yeasen) and the cell cycle were detected by flow cytometry analysis (BD FACSCanto).
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bioRxiv - Bioengineering 2023Quote: Cellular toxicity assays in primary T cells were performed 72 hours post-transduction using the Annexin V:PE Apoptosis Detection Kit (BD Biosciences, 559763). In brief ...
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bioRxiv - Neuroscience 2023Quote: Cells were directly sorted into 1.5 ml tubes containing 100 µl of extraction buffer (XB, Arcturus PicoPure RNA isolation kit) with a FACSAria III SORP (BD Biosciences) flow cytometer equipped with a 90 µm-diameter nozzle ...
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bioRxiv - Immunology 2023Quote: ... Medium was exchanged after 48 h of incubation and after 72 h cells were washed and permeabilized/fixed using Cytofix/Cytoperm kit (BD Biosciences). Cells were stained with VL-4 hybridoma cell line supernatant containing LCMV NP-specific antibodies for 60 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 10-μM bromodeoxyuridine (BrdU) solution was added 4 h before the end of the experiment [fluorescein isothiocyanate (FITC) BrdU Flow Kit (BD #559619)] according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... or (ii) PE-Annexin V conjugated antibody (1:33 dilution in TBST) and 7-AAD as per kit instructions (BD Pharmingen). Samples were analysed by flow cytometry within 30 min.
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bioRxiv - Immunology 2023Quote: ... Cytokine concentrations in the supernatants of DC cultures were measured using a BD human inflammatory cytometric bead array kit (BD #551811) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and either resuspended in PBS and directly analyzed or fixated using the BD Cytofix/Cytoperm fixation and permeabilization kit (BD Bioscience) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Afterwards cells were washed twice with PBS and permeabilized 20 minutes with 200µl BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (BD Biosciences)at RT ...
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bioRxiv - Cancer Biology 2023Quote: Cell cycle analysis was performed by in vitro labeling of cells with the APC BrdU Flow Kit (cat. no. 552598, BD Biosciences). First ...
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bioRxiv - Immunology 2024Quote: ... This was followed by fixation and permeabilization using either the FOXP3 staining buffer set (eBioscience, 00-5523-00) or the Cytofix/Cytoperm kit (BD, 554714). Intracellular staining was done using the following antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... Samples requiring only intracellular cytokine staining were fixed for 20 min on ice using the BD Cytofix/Cytoperm kit (BD 554714) before washing and intracellular staining for at least 1 hr at 4°C ...
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bioRxiv - Genomics 2024Quote: ... RoCKseq modified beads and unmodified beads used for controls were incubated with fluorescent oligos for 30 minutes at 46° C in BD Rhapsody lysis buffer (part number 650000064, BD RhapsodyTM Enhanced Cartridge Reagent Kit, BD 664887) with 1 M DTT (part number 650000063,BD RhapsodyTM Enhanced Cartridge Reagent Kit ...
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bioRxiv - Neuroscience 2024Quote: ... fixed and permeabilized with the FoXP3 Transcription Buffer Kit (eBioscience) and intracellularly stained with PE-CF594-conjugated anti-Foxp3 (BD Biosciences) and PE-conjugated anti-IL-10 (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilized using a BD Cytofix/ Cytoperm Fixation/ Permeabilization Solution kit (Cat# 554714 BD Biosciences, Franklin Lakes NJ) followed by intracellular staining with anti-IFNγ ...
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bioRxiv - Microbiology 2024Quote: ... Efficient induction of definitive endoderm was confirmed by flow cytometry analysis of c-Kit and CXCR4 co-expression using a FACS Symphony A5 instrument (BD Biosciences). NKX2.1+ distal lung progenitor cells were isolated via fluorescently-activated cell sorting (FACS ...
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bioRxiv - Immunology 2024Quote: ... Intracellular expression of TLR3 or TLR7 was determined following fixation and permeabilization using a Cytofix/Cytoperm Kit (BD Bioscience Catalog # 554655). All antibodies were diluted 1:200 in FACS buffer for surface staining or perm buffer for intracellular staining ...
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bioRxiv - Cancer Biology 2024Quote: Induction of apoptosis in tumor cells treated with NPRL2 vector and NPRL2 stably expressing cells (A549-NPRL2++/++ and H1299-NPRL2++/++) with or without carboplatin treatments was analyzed with fluorescence labeled Annexin V and PI staining Kit (BD Biosciences) by flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... the indicated cytokine cytometric beads and PE-conjugated detection antibodies from the BD Cytometric Bead Array (CBA) Human Inflammatory Cytokines Kit (BD Bioscience) were mixed with LPS treated THP-1 supernatants according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cells were washed twice with PBS/0.1% BSA and either resuspended in PBS and directly analyzed or fixated using the BD Cytofix/Cytoperm fixation and permeabilization kit (BD Bioscience) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants of homogenates were collected and cytokines in the samples were analyzed using BD cytometric Th1/Th2/Th17 kit (BD Biosciences). The kit was used for the simultaneous detected of mouse interleukin-2 (IL-2) ...