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Citations for Becton, Dickinson and Company :
6251 - 6300 of 6484 citations for Mouse Bcl 2 Modifying Factor BMF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized following manufacturer protocol using Cytofix/Cytoperm™ Fixation/Permeabilization Kit (BD Biosciences, cat #554714). After centrifugation and washing ...
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bioRxiv - Immunology 2023Quote: ... followed by fixation and permeabilization using the Fixation/Permeabilization buffer kit according to manufacturer’s instructions (BD Biosciences, NJ, USA). This was followed by staining with anti-phospho-Akt antibody diluted in Perm/Wash buffer for 1h ...
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bioRxiv - Neuroscience 2023Quote: ... astrocytes and endothelial cells (LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, ThermoFisher; CD45.1 clone A20 in BV421 (BD); CD45.2 clone 104 in BV421 (BD) ...
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bioRxiv - Genomics 2024Quote: ... BD Rhapsody beads (“Enhanced Cell Capture Beads V2”, Part Number 700034960, BD RhapsodyTM Enhanced Cartridge Reagent Kit, BD 664887) were incubated with a splint ...
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bioRxiv - Microbiology 2022Quote: ... Cell quantities were quantified with flow cytometry using the BD Cell Viability Kit with BD Liquid Counting Beads (BD Biosciences). Cultures were then centrifuged at 3,000 g for 15 minutes and washed with sterile PBS three times before dilution into sterile water to a final concentration of 9x105 cfu/mL.
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bioRxiv - Immunology 2021Quote: ... fixed and stained with APC anti-BrdU antibody and 7-AAD using the APC BrdU Flow kit (BD Pharmingen, 552598) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Apoptosis of the CTAC cells were determine by using FITC-Annexin V Apoptosis Detection kit (BD Pharmingen, San Diego, CA). The cells were treated with 5 μM of plumbagin for 18 h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Single cells were sorted into the 96-well plates of the Precise WTA Kit with predispensed library chemistry using a BD FACSAriaII SORP (BD) and a gating strategy as displayed in Supplementary Figure 3 ...
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bioRxiv - Immunology 2019Quote: Infection-inflicted early cell death was quantified by FACS analyses subsequent to staining of MEFs with FITC/Annexin-V and PI using apoptosis detection kit from BD Biosciences (# 556547) ...
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bioRxiv - Cancer Biology 2019Quote: ... the apoptosis of PANC-1 cells was determined by using the Annexin V-FITC/PI apoptosis detection kit (BD Biosciences). The cells were harvested with trypsin-EDTA (Gibco ...
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bioRxiv - Bioengineering 2020Quote: ... primary human T cells were heated in the thermal cycler prior to assaying with an apoptosis detection kit (BD # 556547) or CellTrace Violet (Fisher # C34571) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed twice with ice-cold PBS and FITC-annexin-PI was done as per manufacturer’s instruction using FITC Annexin V Apoptosis Detection Kit I (Cat. 556547, BD Biosciences). Flow cytometry was performed using a BD LSR II flow cytometer of the Flow Cytometry Core Facility at The Bloomberg School of Public Health ...
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bioRxiv - Microbiology 2020Quote: ... washed twice with cold PBS and then re-suspended in 1x binding buffer(Apoptosis detection Kit I # 556578, BD Pharmingen) at 1x 106 cells/ml ...
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bioRxiv - Immunology 2021Quote: ... lung single cell suspensions were stained with tetramer prior to the surface and intracellular staining for 1 hour at 37°C.Intracellular cytokine staining was performed using the BD Cytofix/Cytoperm kit (BD Biosciences) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... buffer (PBS containing 0.1% sodium azide and 1% bovine serum albumin) and fixed/permeabilized using a Cytofix/Cytoperm Buffer Kit (BD Biosciences) or a FoxP3 Transcription Factor Buffer Kit (eBioscience) ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... Cells were washed with Perm/Wash and stained with 1µl/1mio cells anti-BrdU from the FITC BrdU Flow Kit (BD Biosciences) for 20min at RT in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were stained with the fixable LIVE/DEADTM yellow stain according to the manufacturer’s protocol followed by fixation using the Fixation/Permeabilization kit (BD Biosciences). After fixation ...
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bioRxiv - Cell Biology 2021Quote: ... conjugated anti-c-kit and Peridinin Chlorophyll Protein Complex (PerCP) or allophycocyanin (APC) conjugated anti-CD45 antibodies were purchased from BD Biosciences Pharmingen ...
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bioRxiv - Cancer Biology 2022Quote: ... for 45 minutes and harvested for analysis of BrdU positive cells using the BrdU flow kit according to manufacturer’s protocol (BD Biosciences). Nuclei were stained with 7-AAD ...
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bioRxiv - Genomics 2022Quote: ... Obtained single-cell suspensions were subjected to a BD Rhapsody system with BD Rhapsody Targeted & Abseq Reagent kit (BD Biosciences) following the manufacture’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and live/dead fixable NIR dead cell stain kit reagent according to manufacturer’s directions and analysed on a 5-laser Fortessa X20 (BD Biosciences) as previously described (Zaunders et al. ...
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bioRxiv - Immunology 2022Quote: ... Intracellular cytokine staining was performed by activating cells for 4-5h at 37°C with the Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) and subsequent fixing and staining per manufacturer’s instructions using the BD Fixation/Permeabilization Solution Kit (BD Biosciences). For intranuclear transcription factor staining ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... NK cell-specific surface markers were stained and cells were fixed and permeabilized using the Cytofix/Cytoperm Kit (BD Biosciences) before adding anti-IFN-γ and anti-TNF-α ...
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bioRxiv - Immunology 2022Quote: ... Flow cytometric evaluation of intracellular cleaved caspase-3 was carried out using the FITC Active Caspase-3 Kit (BD Bioscience) and CD19 staining after 24 hour co-culture at a 1:4 E:T ratio.
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bioRxiv - Cell Biology 2019Quote: ... APC-conjugated anti-c-kit/CD117 (generated by the WEHI Antibody Facility) and PE-Cy7-conjugated anti-Sca1 (BD Pharmingen). Cells were stained with FluoroGold to assess viability and analysed on a BD LSRFortessa cell analyser.
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bioRxiv - Zoology 2020Quote: The apoptosis of erythrocyte was determined using PE annexin-V apoptosis detecting kit according to the manufacturer’s instruction (BD, USA). In brief ...
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bioRxiv - Immunology 2020Quote: ... antibodies were used for intra-cellular staining by using the Fixation/Permeabilization Solution Kit (BD Cytofix/Cytoperm™, BD Biosciences). For in vitro intracellular cytokine staining ...
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bioRxiv - Immunology 2020Quote: ... antibodies were used for intra-cellular staining by using the Fixation/Permeabilization Solution Kit (BD Cytofix/Cytoperm™, BD Biosciences). For in vitro intracellular cytokine staining ...
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bioRxiv - Immunology 2019Quote: ... The number of CD11b receptors on CD45+CD11b+ TAMCs was quantified using the QuantiBRITE PE Fluorescence Quantitation Kit (BD Biosciences), according to the manufacturer’s recommended protocol.
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bioRxiv - Immunology 2020Quote: ... BV421 conjugated streptavidin was added for 20 minutes at 4 °C prior to fixation and permeabilisation (BD Fix perm kit). Anti IFN-γ-FITC (clone CC302 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were fixed and permeabilized using BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit according to the manufacturer’s protocol (BD Biosciences). Cells were then incubated with anti-vaccinia virus primary antibody (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... both floating and adherent cells were harvested and analysed with the FITC Annexin V Apoptosis Detection Kit I (BD Bioscience), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Apoptotic cells were detected by Accuri C6 fluorescence-activated cell sorting (FACS) using Annexin V-APC Apoptosis Detection Kit (BD #), according to the manufacturers protocol.
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bioRxiv - Cancer Biology 2020Quote: ... to analyze cell cycle distribution and we quantified apoptotic cells with PE-AnnexinV Apoptosis Detection Kit I (559763, BD Pharmingen).
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bioRxiv - Developmental Biology 2021Quote: ... Single cell whole transcriptome analysis libraries were prepared according to the manufacturer’s instructions using BD Rhapsody WTA Reagent kit (BD, 633802) and sequenced on the Illumina NextSeq 500 using High Output Kit v2.5 (150 cycles ...
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bioRxiv - Cancer Biology 2021Quote: Viability of AML cells was measured using FITC Annexin V Apoptosis Detection Kit I (BD Biosciences, San Jose, CA, USA). Briefly ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for viability and surface markers followed by intracellular cytokine staining using the Cytofix/Cytoperm kit from BD Biosciences (Franklin Lakes ...
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bioRxiv - Immunology 2021Quote: In vivo cytokines from the serum/plasma of mice were measured using the Cytometric Bead Array Human Th1/Th2 Cytokine Kit (BD), following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: Cell apoptosis assay was performed by flow cytometry using an Annexin V-FITC /propidium iodide(PI) apoptosis detection kit(BD). Cells were seeded in triplicates in 6-well plate at the density of 2×105 cell per well ...
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bioRxiv - Cancer Biology 2022Quote: ... and MDAMB231-NS cells separately with the Sample-Tags from the BD Human Immune Single-Cell Multiplexing Kit (BD Biosciences) as described in the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... with LCMV Cl13 and BrdU detection in splenic P14 cells was performed one day later (d8p.i.) using a BrdU detection Kit (BD Bioscience) according to manufacturer’s protocol.
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bioRxiv - Genomics 2022Quote: ... Single cell capture and cDNA synthesis using the Single Cell Capture and cDNA Synthesis kit (#633731, #633733, #633773) were done following the manufacturer’s protocol (BD Biosciences). Libraries were then prepared using the BD Rhapsody WTA Amplification Kit (#633801 ...
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bioRxiv - Immunology 2022Quote: ... Cells were next washed twice with cold 1x Perm/WashTM buffer (Cytofix/Cytoperm™ fixation and permeabilization kit, BD Biosciences) and incubated with a master mix containing 95μL of 1x Perm/WashTM buffer and 5μL of the intracellular marker Ki-67 (Table 2 ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was collected to determine the levels of MCP-1/CCL2 using commercial kit (BD Biosciences, CA, USA, Cat# 555179) as per manufacturer’s protocol.
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... Anti-TCR Vα2-PE (# 553289), anti-CD8α-BUV395 (# 563786), Stain Buffer (FBS, # 554656) and BD Cytofix/Cytoperm kit (# 554714) were purchased from BD Biosciences ...
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bioRxiv - Immunology 2024Quote: Plasma samples isolated from blood collected at day 7 and previously frozen at -20°C were thawed and pooled to reach the minimum sample volume required for cytokine analysis (20μL) using the Th1/Th2/Th17 cytokine kit (BD Biosciences). Data were analyzed using the FCAP array software ...
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bioRxiv - Developmental Biology 2024Quote: ... Single cell whole transcriptome analysis libraries were prepared according to the manufacturer’s instructions using BD Rhapsody WTA Reagent kit (BD, 633802) and sequenced on the Illumina NextSeq 500 using High Output Kit v2.5 (150 cycles ...
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bioRxiv - Neuroscience 2024Quote: ... Intracellular markers were stained after fixation and permeabilization of cells using an Intracellular or Phospho-Flow Staining kit (BD Biosciences). FACS data were acquired on a Northern Lights spectral flow cytometer (Cytek ...
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bioRxiv - Microbiology 2024Quote: ... Secreted cytokines were measured using a BD Th1/Th2/ Th17 cytometric bead array (CBA) kit (BD Biosciences cat no. 560485) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed twice with ice-cold PBS and FITC-annexin-PI was done as per manufacturer’s instruction using FITC Annexin V Apoptosis Detection Kit I (Cat. 556547, BD Biosciences). Flow cytometry was performed using a BD LSR II flow cytometer of the Flow Cytometry Core Facility at The Bloomberg School of Public Health ...