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Citations for Becton, Dickinson and Company :
5801 - 5850 of 8884 citations for 7 Chloro 3 methyl 3H 1 2 3 triazolo 4 5 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... The following primary antibodies were used: anti-Egr 1:50 (generous gift from Konrad Basler) and anti-actin 1:3000 (Cat # 612656 from BD Biosciences). For quantification ...
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bioRxiv - Cancer Biology 2021Quote: ... or 1 × 106 SK-Mel-24 cells and 1 × 106 uninduced GFP/M27 or bcat-GFP/M27 in 50 µl of growth-factor-reduced Matrigel/PBS (1:1) (BD Biosciences) was injected intradermally into the flanks of 4-6-week-old NOD.Cg-Prkdcscid/J mice (Jackson Laboratory ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Synapsin (1:500) and anti-CD133 (1:500) were used after Fc blocking with CD16/32 antibody (553141; BD Pharmingen). Respective Secondary antibodies ...
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bioRxiv - Immunology 2020Quote: ... The cells were fixed with 4% neutrally buffered formalin for 5 min and stained with anti-mouse primary antibodies (1:100, anti-mouse LC3B, LAMP-1 and p62 antibodies) followed by a biotinylated anti-Ig secondary antibody (BD Biosciences) and streptavidin-PE ...
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bioRxiv - Immunology 2021Quote: ... the sera were diluted 1/10 with PBS containing 1% (w/v) BSA and loaded onto anti-mouse IgE coated (BD biosciences) plates anti-OVA-HRP antibodies (AbD Serotec ...
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bioRxiv - Immunology 2020Quote: ... stimulated with 1 μg/ml LCMV NP118-126 peptide for 1 hour and then in the presence of GolgiPlug (BD Biosciences) and peptide for an additional 4 hours ...
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bioRxiv - Genetics 2020Quote: ... Fluorescence activated nuclei sorting was performed until at least 400,000 nuclei were collected for each cell type (NeuN+, Pu.1+, and NeuN-/Pu.1-) using the FACSAria (BD Biosciences, US).
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bioRxiv - Cancer Biology 2021Quote: ... A total of 5 million cells were suspended in 150 μL of a 1:1 v/v mixture of medium with reconstituted basement membrane (BD Matrigel, BD Biosciences) and injected subcutaneously in each mouse.
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bioRxiv - Immunology 2021Quote: ... mMDSCs (CD11b+Gr-1+Ly6G-) and gMDSCs (CD11b+Gr-1+Ly6G+) were sorted into FACS buffer using a BD FACSMelody (BD Bioscience). After centrifugation at 400 g for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... Unfractionated BM was diluted 1:1 in DMEM with 10% FBS and added to vacuum gas plasma treated plates (BD Falcon). After three days ...
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bioRxiv - Neuroscience 2022Quote: ... that was blocked in 5% non-fat milk for 1 h at room temperature and incubated overnight with primary antibodies against the following proteins: α-synuclein (1:1000, BD), NCK2 (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were stained with the antibody cocktail in the staining buffer of a 1:1 mix of PBS and Brilliant Staining Buffer (BD, 566349), at room temperature for 20 mins or at 4°C for 30-40 mins ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 × 106 VCaP cells were subcutaneously injected into the dorsal flank on both sides of each mouse in a 1:1 mixture of serum free medium and Matrigel (BD Biosciences). Once tumors grew to a palpable size (∼400 mm3) ...
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bioRxiv - Microbiology 2023Quote: ... for 16 hours followed by stimulation with a peptide pool of a broad range of HLA-subtypes and infectious agents (CEFX Ultra SuperStim Pool; JPT) (1 µg/ml) and 1 μg/ml mouse-anti-human CD28 antibody (#556620; BD Biosciences), or IL-2 (20 IU/ml ...
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bioRxiv - Neuroscience 2023Quote: ... and antibodies directed against intracellular targets Foxp3 (APC, eBioscience #17-5773, 1:200) and RORγt (BV421, BD Biosciences #562894, 1:200). After 1h incubation at 4°C ...
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bioRxiv - Immunology 2024Quote: ... PMA [1μg/ml] and ionomycin [0,5μg/ml] with Golgi Plug [1:1000] and Golgi Stop [1:1000] solutions (Cytofix/Cytoperm TM Plus, BD Bioscience, ref: 555028) for 4-5h at 37°C to block Golgi’s exocytosis ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed 2x with 1% FBS in PBS and incubated in 1% FBS in PBS with APC-conjugated mouse anti-human CD68 (BD Biosciences) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cells were stained intracellular with anti-Foxp3 Alexa Fluor 488 (eBioscience; 1/100) and anti-ROR t BV421 (BD; 1/100). Flow cytometry data were analyzed using FlowJo Software 10.8.1 and a sequential gating strategy ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for 30 min on ice with biotinylated recombinant HAs and PD-1-BB515 (EH12.1, BD Horizon, 1:100) diluted in P2 ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed with buffer (0.2% FBS in 1× PBS) and data (∼1 million events/sample) was acquired using BD LSRFortressa X-20 (BD Biosciences, USA). The acquired flow cytometry data were analysed using FCS Express (DeNovo software ...
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bioRxiv - Cancer Biology 2023Quote: ... were anesthetized with isofluorane and GFP-Luc labelled cells or MCF7-HypT cells resuspended in 1:1 PBS and Matrigel (BD Biosciences) were injected into the fourth mammary fat pad ...
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bioRxiv - Molecular Biology 2023Quote: ... For BrdU staining we used either a rat anti-BrdU (1:1000, Eurobio clone BU1/75) or mouse anti-BrdU (1:1000, BD 347580) antibodies 2 hours at RT (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... together with PE-anti-PD-L2 (clone TY25, 1:20) and its isotype control PE-Rat IgG2a,κ (clone R35-95, 1:20) were purchased from BD Biosciences ...
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bioRxiv - Developmental Biology 2022Quote: ... 1:10000), anti-dendritic cell mAb: anti-CD11c (N418)-FITC (11-0114-81, eBioscience; 1:20) using LSR II (BD Biosciences). For OC differentiation ...
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bioRxiv - Cancer Biology 2023Quote: ... coverslips were incubated with primary antibodies (mouse anti-HA 1:200, Cell Signaling Technology, 2367; mouse anti-CtBP2, 1:250; BD Biosciences Cat# 612044 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5.0 × 104 MDA-MB-231 or HCC1806-LM2C triple reporter-labeled cells were resuspended in a 1:1 mixture of PBS and Matrigel (BD Biosciences), and injected into the 4th mammary fat pad of female NOD-SCID-g female mice 6-8 weeks of age ...
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bioRxiv - Microbiology 2023Quote: ... Crypts in the tube were collected after removing the supernatant, and resuspended in 50% L-WRN conditioned medium (CM, Table S3) mixed 1:1 with Matrigel (BD Biosciences). The crypt suspension (55 μl per well ...
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bioRxiv - Cancer Biology 2023Quote: ... and/or 0.25 million A431 cells in a 100 μL cell suspension of a 1:1 (v/v) mixture of medium with reconstituted basement membrane (BD Matrigel, BD Biosciences). Mice-bearing NCIN87/A431 bilateral tumors were developed by subcutaneous injection of NCIN87 and A431 cancer cells on the bilateral dorsal flank regions ...
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bioRxiv - Immunology 2023Quote: ... and anti-MCL-1 A647 (1:1000, clone 19C4-15, WEHI antibody facility) and analysed on a Fortessa X20 (BD Biosciences) or Cytek Aurora spectral flow cytometer (Cytek Biosciences) ...
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bioRxiv - Physiology 2024Quote: ... The 2X stain cocktail included the following antibodies (final dilutions are all 1:300 unless otherwise indicated): rat anti-mouse SiglecF-BV421 (1:400, clone E50-2440, cat# 562681 BD Horizon), rat anti-mouse/human CD11b-Pacific Blue (clone M1/70 ...
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bioRxiv - Immunology 2024Quote: ... The cells were resuspended in T cell stimulation media (RPMI 1640 supplemented with 10% FBS, 1% Penicillin/Streptomycin, Protein Transport Inhibitor (Containing Brefeldin A) (diluted 1:1000) (BD, 555029), Monensin (Biolegend ...
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bioRxiv - Microbiology 2024Quote: ... washed with PBS-T and kept overnight in 1% BSA solution in PBS-T containing a 1:500 dilution of anti-rabbit-Vi-polysaccharide antibody (BD BioSciences) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... cells were washed and resuspended in PBS/2% FBS for analysis on a five-laser flow cytometer (FACSymphony A3 Cell Analyser, BD Bioscience, San Jose, CA, USA). Data were analyzed with FlowJo software (TreeStar ...
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bioRxiv - Neuroscience 2020Quote: Neurons from 18-day-old rat embryos were dissected and dissociated using trypsin and plated at a density of 70,000 cells cm−2 in minimal essential medium (MEM) supplemented with 10% NU serum (BD Biosciences, Le Pont de Claix, France), 0.45% glucose ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we allowed organotypic cultures to differentiate at an air-liquid interface (ALI) in a defined differentiation media [base media with 2% v/v NuSerum (BD BioSciences, San Jose, CA, USA), and 50 nM retinoic acid (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we allowed organotypic cultures to differentiate at an ALI in a defined differentiation media supplemented with IL-13 [base media with 2% v/v NuSerum (BD BioSciences, San Jose, CA, USA), 50 nM retinoic acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: The actinobacteria were routinely cultured from frozen spore preparations via inoculation into Difco International Streptomyces Project (ISP) medium 2 (ISP2) (Becton, Dickinson and Company, Spark, MD) and incubated at 28° C ...
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bioRxiv - Immunology 2020Quote: ... before being washed and re-suspended in PBS/2% FCS for analysis on a five-laser flow cytometer (LSR FortessaTM; BD Bioscience, San Jose, CA, USA). The data were analysed with FlowJo software (TreeStar ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... BD 560046. After washing twice, cells were fixed in PFA 2% for 20 min, washed, and acquired on a Fortessa BD LSRII Cell Analyzer (Becton Dickinson).
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bioRxiv - Bioengineering 2023Quote: ... Cells were then washed 3x in flow cytometry stain buffer and fixed with 2% PFA for 20 minutes prior to acquisition on a LSRII Fortessa (BD Biosciences, San Jose, CA, USA). Samples were analyzed with FlowJo V10 software (Figure S4).
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bioRxiv - Cancer Biology 2023Quote: ... or E0771 hPD-L1 (2 × 105 cells in 25 μL of medium mixed with 25 μL of Matrigel Basement Membrane Matrix [BD Biosciences, San Jose, CA, USA]) were injected into the mammary fat pad or flank ...
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bioRxiv - Immunology 2023Quote: ... 5.0 × 104 CD4+ T cells were serially diluted onto either α-IL-2 or α-IFNɣ sterile coated PVDF 96 well plates (BD Biosciences, NJ, USA). The CD4+ T cells were then activated for 24 or 72 h in complete DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... PMVECs were purified after two rounds of immune selection using magnetic beads coated with anti-CD31 and anti-ICAM-2 antibodies (BD Bioscience, Franklin Lakes, NJ, USA). mPMVECs were cultured and maintained in a medium containing 5 % FBS and EC growth supplement (ECGS ...
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bioRxiv - Genetics 2022Quote: ... The proportion of HbF-and HbS-positive RBCs were measured with an antibody recognizing HbF (Thermo Fisher for HUDEP-2 cells or BD Biosciences for primary cells) or HbS (BioMedomics) ...
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bioRxiv - Immunology 2020Quote: Multiscreen ninety-six well plates were coated overnight with 100 μl per well of 5 μg/ml anti-human interferon-γ (IFN-γ) (B27; Becton, Dickinson and Company, Franklin Lakes, NJ) in endotoxin-free Dulbecco’s-PBS (D-PBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in 50 µL of FACS buffer (PBS plus 1% BSA) and blocked with µL 1 human Fc block (BD Biosciences, 564219) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 30 min on ice with biotinylated and Alexa Fluor 647-conjugated recombinant soluble S and PD-1-BB515 (EH12.1, BD Horizon, 1:100) in 2% FBS and 2 mM EDTA in PBS (P2) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were fixed and permeabilized prior to staining with primary antibody (Vimentin CST # 1:100 and E-cadherin BD number 1:100) overnight followed by secondary (anti-rabbit ThermoFisher #31466 1:10000 ...
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bioRxiv - Neuroscience 2019Quote: ... Blocking was followed by 1-hour incubation with Anti-Pan Nav (Alomone labs, # ASC003) (1:50) and purified Mouse Anti-MAP II (BD biosciences, #556320) (1:400 ...