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Citations for NanoTemper :
401 - 450 of 604 citations for NT proBNP Protein Recombinant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: The thermophoretic measurements were performed using Monolith NT.115 device with red detection channel (NanoTemper Technologies, Munich, Germany). Recombinant Spikeplug was produced in our laboratory ...
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bioRxiv - Neuroscience 2021Quote: ... True label-free fluorescence measurements were performed on a Prometheus NT.48 nanoDSF instrument (NanoTemper Technologies, Munich, Germany) using nanoDSF grade standard capillaries (10μL ...
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bioRxiv - Molecular Biology 2020Quote: ... The thermophoretic properties were of the labelled SecB were then determined with a Monolith NT.115 (Nanotemper Technologies) using MST Premium Coated Capillaries at 100% MST power ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting mixtures were loaded into a Monolith NT.115 series standard capillaries (NanoTemper, catalogue no. MO-K002). Thermophoresis experiments were performed with 40% of MST power and 20% of LED power for fluorescence acquisition.
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bioRxiv - Plant Biology 2022Quote: ... and a melting curve was determined by using the nanoDSF Prometheus NT.48 instrument (NanoTemper GmbH, Munich, Germany) set to an LED power of 60% to 80% ...
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bioRxiv - Molecular Biology 2022Quote: All microscale thermophoresis measurements (MST) were performed on a NanoTemper Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). using the Standard Treated Capillaries K002 of the supplier ...
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bioRxiv - Molecular Biology 2022Quote: The kinetic measurements of PfCoSP-gDNA binding were conducted using Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). 10 μM PfCoSP was labeled using NanoTemper’s Protein Labeling Kit RED-NHS (L001 ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were loaded using capillaries in a volume of 10µL on Prometheus Panta NT.48 from NanoTemper Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Then 20 μL sample was loaded into standard treated capillaries for measurements on a Monolith NT Automated (Nanotemper).
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bioRxiv - Biochemistry 2023Quote: Binding experiments were performed using microscale thermophoresis (MST).39 Experiments were performed on a Monolith NT.115 (NanoTemper) using 20% light-emitting diode power ...
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bioRxiv - Microbiology 2024Quote: All microscale thermophoresis measurements (MST) were performed on a NanoTemper Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). using the Standard Treated Capillaries K022 of the supplier ...
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bioRxiv - Biophysics 2024Quote: ... Samples were loaded into capillaries and analyzed using a Monolith NT.115 system (Nanotemper Technologies GmbH, Munich, Germany) with an excitation power of 20% and 40% MST power ...
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bioRxiv - Biophysics 2019Quote: MicroScale Thermophoresis (MST) was performed using Monolith NT.115 Label Free system through the MO.Control software (NanoTemper Technologies, Germany). All measurements were carried out in 10 mM acetate pH 5.0 at 25°C and two different ligands were chosen ...
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bioRxiv - Biophysics 2021Quote: Thermal stability and binding affinity were determined using nanoscale differential scanning fluorimetry on a Prometheus NT.48 (NanoTemper Technologies). Capillaries were filled with 10 μL of respective samples in duplicates and loaded into the reading chamber ...
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bioRxiv - Biochemistry 2022Quote: Thermal unfolding of GDAP1 variants in SEC buffer was studied by nanoDSF using a Prometheus NT.48 instrument (NanoTemper). Tryptophan fluorescence was excited at 280 nm ...
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bioRxiv - Microbiology 2022Quote: ... The labeled protein was 1:1 incubated with a series dilution of ABT199 (4 uM to 0.122 nM) to perform the microscale thermophoresis experiments in Monolith NT.115 (NanoTemper) with the setting of 40% MST power and 40% excitation power at 25 °C ...
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bioRxiv - Microbiology 2022Quote: ... to 0.1 µM purified ClpC1NTD-FITC was assayed in CPD supplemented with 0.05% Tween 20 and 8 mg/ml BSA by microscale thermophoresis using a Monolith NT.115 (NanoTemper) (Jerabek-Willemsen et al. ...
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bioRxiv - Systems Biology 2021Quote: ... The sample was then loaded into standard treated capillaries and analyzed using a Monolith NT.115 (NanoTemper Technologies Germany). The blue laser was used for a duration of 35 s for excitation (MST power = 60% ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Experiments were run at 20% excitation and high MST power at 23.0°C on a Monolith NT.115Pico (NanoTemper). Each experimental compound was run in triplicate.
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bioRxiv - Molecular Biology 2020Quote: The melting temperature (T m) of sE2 (1mg/ml) was calculated using the Prometheus NT.48 (Nanotemper, Munich, Germany) during heating in a linear thermal ramp (1°C ...
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bioRxiv - Molecular Biology 2020Quote: ... L765F and L765A point mutants in NMR buffer were assessed by nanoDSF using a Prometheus NT.48 instrument (Nanotemper). Samples were heated from 15 °C to 75 °C on a gradient increasing by 2 °C per minute at 30% excitation.
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bioRxiv - Biochemistry 2020Quote: Thermal unfolding of wild-type and C88A GDAP1Δ303-358 in SEC buffer was studied by nanoDSF using a Prometheus NT.48 instrument (NanoTemper). The fluorescence of tryptophan was excited at 280◻nm and recorded at 330◻nm and 350◻nm ...
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bioRxiv - Immunology 2023Quote: All MST experiments were performed on a Monolith NT.115 instrument with green/red filters (NanoTemper Technologies, Munich, Germany). MST and LED power were set at 40% and 60% ...
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bioRxiv - Biochemistry 2023Quote: ... Approximately 10 µL of each sample was loaded into Prometheus NT.48 Series nanoDSF high sensitivity capillaries (NanoTemper, Germany) and melting scans were performed using the Pr.ThermControl software (NanoTemper ...
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bioRxiv - Plant Biology 2023Quote: ... and mixed with different concentrations of barley total RNA (Phenol/Chloroform) before loading onto Monolith NT.115 (NanoTemper Technologies). Data were treated by the KD Fit function of the Nano Temper Analysis Software (version 1.5.3).
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bioRxiv - Bioengineering 2023Quote: ... The samples (10 µL at 0.2 mg/mL in HBS) were loaded into standard treated NT.115 capillaries (NanoTemper), and absorption at 330 and 350 nm was measured on a Prometheus NT.48 instrument (NanoTemper ...
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bioRxiv - Microbiology 2023Quote: Nucleotide binding assays based on microscale thermophoresis were performed with a Monolith NT.115 instrument (NanoTemper Technologies GmbH, Germany) using Monolith NT Premium Capillaries ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and centrifuged at 14,000 rpm for 10 min at 4°C before loading into Monolith NT.115 premium capillaries (NanoTemper). Samples were loaded into the Monolith NT.115 device (NanoTemper ...
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bioRxiv - Cell Biology 2020Quote: ... and allowed to incubate in the dark for 30 min before loading into NT.115 premium treated capillaries (Nanotemper Technologies). A red LED at 10% excitation power (red filter ...
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bioRxiv - Microbiology 2021Quote: The thermal stability of wild-type HigBHigA and HigB(L5ext)HigA were assessed using a Tycho NT.6 instrument (NanoTemper). Protein was heated at 0.1°C steps over a temperature range of 35°C to 95°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fluorescently labeled 100 nM NKR-P1 was mixed with dilution series of LLT1 or LLT1SIM and analyzed in standard capillaries in an NT.115 Monolith (NanoTemper) using 30% LED excitation power and 60% MST power ...
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bioRxiv - Molecular Biology 2020Quote: ... Intrinsic protein fluorescence at 330 nm and 350 nm was monitored between 15 and 90 °C in the Prometheus NT.48 instrument (Nanotemper), and the Tm values calculated within the accompanying software by taking the turning point of the first derivative of the F350:F330 ratio as a function of temperature.
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bioRxiv - Molecular Biology 2021Quote: ... 20 µL reactions were incubated at room temperature for 5 min and 5 µL of the mixture was loaded into NT.115 capillaries (NanoTemper). MST was performed in Monolith NT.115 (NanoTemper ...
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bioRxiv - Biochemistry 2019Quote: ... was monitored during unfolding over a linear temperature gradient (35-95 °C) over 3 minutes on a Tycho NT.6 instrument (NanoTemper). The temperature at which a transition occurs in a plot of the first derivative of the fluorescence ratio (350/330 nm) ...
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bioRxiv - Biochemistry 2020Quote: DSF assays were performed on LH3 wild-type and mutants using a Tycho NT.6 instrument (NanoTemper Technologies GmbH, Germany). LH3 samples at a concentration of 1 mg/mL in a buffer composed of 25 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... The reaction mixtures were taken up into MST capillaries and measurements were acquired using a Monolith NT.115 (NanoTemper Technologies) microscale thermophoresis instrument ...
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bioRxiv - Biochemistry 2020Quote: The binding affinities of compound 20 to Sc-Dyn-lysoMut was measured using Monolith NT.115 (NanoTemper Technologies, Munich, Germany). Sc-Dyn-lysoMut was labeled with the Monolith NT Protein Labeling Kit RED-NHS ...
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bioRxiv - Plant Biology 2021Quote: ... The intrinsic fluorescence signal was measured as a function of increasing temperature in a Prometheus NT.48 fluorimeter (Nanotemper™), with 55% excitation light intensity and 1 °C/minute temperature ramp ...
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bioRxiv - Plant Biology 2020Quote: ... The intrinsic fluorescence signal was measured as a function of increasing temperature with a Prometheus NT.48 fluorimeter (NanoTemper Technologies), with 55% excitation light intensity and 1 °C/min temperature ramp ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then loaded into 3 standard capillaries for triplicate readings in a Prometheus NT.48 instrument (Nanotemper Technologies, Munich, Germany). Experiments were run using 40% excitation power ...
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bioRxiv - Biochemistry 2021Quote: Differential scanning fluorimetric analysis of PaPlaB thermal stability was performed using the Prometheus NT.48 nanoDSF instrument (NanoTemper Technologies, Germany) [39] ...
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bioRxiv - Biophysics 2020Quote: The binding affinity of spike to Sb23 was measured by microscale thermophoresis using the MST NT.115 device (NanoTemper Technologies). Sb23 was labelled via the His6-tag with RED-tris-NTA dye (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... TmrAB was diluted to 8.5 μM in SEC buffer and prepared in duplicate for measurement on a Tycho NT.6 (NanoTemper Technologies). Data are reported as the ratio of fluorescence intensity at 350 nm and 330 nm normalized to the minimum and maximum values of the whole dataset.
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bioRxiv - Biophysics 2021Quote: ... Protein samples with a concentration ranging between 0.5 and 1.0 mg/ml were measured in triplicates in quartz capillaries (Prometheus NT.48 Series nanoDSF grade standard capillaries, NanoTemper Technologies). Fluorescence measurements were made with an excitation wavelength of 280 nm ...
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bioRxiv - Biophysics 2019Quote: ... Nucleoprotein samples in PBS buffer solution (at concentrations of 3 mg/ml) were loaded into quartz capillaries (Standard Capillaries Prometheus NT.48, NanoTemper); capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
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bioRxiv - Biochemistry 2021Quote: The stability of PfGSK3 under different buffer conditions and additives was measured by nanoDSF (Prometheus NT.48, NanoTemper Technologies, GmbH). The protein concentration was adjusted to 1 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Quality control during protein purification was carried out using reducing and non-reducing SDS-PAGE analysis and differential scanning fluorimetry (DSF) with a Tycho NT.6 instrument (Nanotemper). All samples were concentrated to 1 mg mL-1 ...
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bioRxiv - Biochemistry 2022Quote: ... and allowed to incubate in the dark for 30 min before loading into NT.115 premium treated capillaries (Nanotemper Technologies). A red LED at 30% excitation power (red filter ...
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bioRxiv - Biophysics 2022Quote: ... HBc/4M2e samples in PBS buffer solution (at concentrations of 3 mg/ml) were collected in glass capillaries (Standard Capillaries Prometheus NT.48, NanoTemper), capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
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bioRxiv - Developmental Biology 2022Quote: Binding assays of purified NHR23 or strep-NHR85 was measured using a Monolith NT.115 Pico running MO Control version 1.6 (NanoTemper Technologies). Assays were performed in 100 mM NaCl ...