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Citations for NanoTemper :
451 - 500 of 604 citations for NT proBNP Protein Recombinant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... NCS-1ΔH10/Ric-8A-P2 peptide and NCS-1ΔH10/Ric-8A-P3 peptide were performed using a Tycho NT.6 instrument (NanoTemper Technologies). Proteins at 10 μM in their corresponding final buffers (see above ...
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bioRxiv - Biophysics 2023Quote: ... 0.2 mM ATP) with 0.05 % Tween-20 on a Monolith NT.115 Microscale Thermophoresis (MST) device using standard treated capillaries (NanoTemper Technologies). Actin (final labelled-actin concentrations adjusted to 60 nM ...
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bioRxiv - Biochemistry 2023Quote: ... and allowed to incubate in the dark for 30 min before loading into NT.115 premium treated capillaries (NanoTemper Technologies). A red LED at 20% excitation power (red filter ...
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bioRxiv - Systems Biology 2023Quote: Thermal unfolding of proteins was measured in absence and presence of metabolites by nano differential scanning fluorimetry (F350/F330) using a Prometheus NT.48 (NanoTemper) at 95% excitation power over a temperature gradient from 20 °C to 95 °C at an increase of 1 °C per minute ...
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bioRxiv - Biochemistry 2023Quote: ... was measured from 15°C to 95°C with a ramp rate of 2°C per minute with a Prometheus NT.48 nano-fluorimeter (NanoTemper). The melting temperatures (Tm values ...
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bioRxiv - Molecular Biology 2023Quote: ... 650 nm fluorescent dye using the Monolith NT RED-NHS lysine labeling kit according to manufacturer’s protocol (NanoTemper, Munich, Germany). RED labeled untagged sP36 at 100 nM and His6-GlnK1 in 16 different concentrations ranging from 7.5 µM to 0.23 nM or 10 nM of sP36-RED and AmtB1-His6 in 16 dilutions ranging from 12.6 µM to 0.38 nM (all concentrations based on monomeric molecular mass ...
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bioRxiv - Biochemistry 2023Quote: ... Thermal stability in the presence and absence of various potential ligands was measured using a Tycho NT.6 instrument (Nanotemper).
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bioRxiv - Biochemistry 2024Quote: ... Measurements were performed in triplets at 25 °C and at medium MST power in Monolith NT.115 Capillaries (NanoTemper Technologies). The raw data were analyzed using the MO.Affinity Analysis software (NanoTemper Technologies).
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bioRxiv - Neuroscience 2023Quote: ... Control experiments included measurements of the binding of the RED-tris-NTA dye to His-DJ-1 and of a dye-labeled AptamerCy5 to AMP using a manufacturer kit (Monolith NT.115 Control Kit RED, NanoTemper).
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bioRxiv - Biochemistry 2024Quote: ... Fluorescence was determined in a thermal gradient at 40% LED power and high MST power generated by a Monolith NT.115 from NanoTemper Technologies ...
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bioRxiv - Biophysics 2020Quote: ... The final reaction mixtures were loaded into premium capillary chips (Monolith Cat# MO-AK005) and measured on a Monolith NT.115 instrument (NanoTemper Technologies) at 20% excitation power and 40% MST power at 25 °C ...
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bioRxiv - Plant Biology 2020Quote: ... and the thermophoretic response was monitored with 30 % infrared (IR) laser intensity and 80 % MST power using a Monolith NT.115 instrument (NanoTemper Technologies) at 20 °C ...
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bioRxiv - Biophysics 2020Quote: ... The equilibrium binding between 20 nM NT-647-NHS labelled hGH to hGHR(MSP1D1) was calculated from the change in thermophoresis ΔFnorm = ΔFhot/ΔFcold measured on a Monolith NT.115 (NanoTemper Technologies). For hGHG120R the raw fluorescence change was used to determine the binding affinity ...
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bioRxiv - Biochemistry 2021Quote: Binding of either guanine or xanthine by purified PurTCp was measured by microscale thermophoresis (MST) using the Monolith NT.LabelFree and Monolith NT.115 (NanoTemper Technologies, Germany) to detect the thermophoretic signals of native tryptophan and tagged fluorescence in PurTCp ...
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bioRxiv - Biophysics 2021Quote: ... Fluorescent measurements were made at excitation 280 nm (UV-LED) and at emission wavelengths of 330 and 350 nm using Prometheus NT.48 (NanoTemper Technologies). Data from duplicate measurements of each sample were processed using PR.ThermControl Software (NanoTemper Technologies).
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bioRxiv - Cell Biology 2021Quote: ... proteins at 200 nM were incubated with varying concentrations of CaCl2 and ZnSO4 in a 10 µl mixture and loaded into NT.115 standard coated capillaries (NanoTemper Technologies). The buffers (25 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... Each reaction mixture was incubated for 10 min in a PCR-tube followed by thermophoresis in standard MST-grade glass capillaries in a Monolith NT.115 instrument (Nanotemper Technologies).
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bioRxiv - Cell Biology 2021Quote: Binding of cevipabulin to the seventh site was detected with an MST assay with a Monolith NT.115 instrument (NanoTemper Technologies). Purified tubulin was labeled using the Monolith protein labeling kit RED-NHS (NanoTemper Technologies ...
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bioRxiv - Synthetic Biology 2022Quote: The GTP and MC-LR affinity of the purified FtsZ from Synechococcus 7942 were measured using the Monolith NT.115 (Nanotemper Technologies)(Dong et al ...
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bioRxiv - Immunology 2022Quote: Thermal unfolding of purified HAssF in PBS was measured by nano differential scanning fluorometry (nDSF) using Prometheus NT.48 instrument (NanoTemper technologies). Thermal unfolding curves are plotted slope (Δ(F350 nm/F330 nm)/ΔT ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... Protein quality control after refolding and SEC was performed by nanoscale differential scanning fluorimetry (nanoDSF) runs (see Figure 4S2) acquired with a Nanotemper Prometheus NT.48 fluorimeter (Nanotemper, Munich) controlled by PR.ThermControl (version 2.1.2).
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bioRxiv - Microbiology 2019Quote: ... Measurements were performed in standard capillaries with a Monolith NT.115 Pico system at 20% excitation power and 40% MST power (NanoTemper Technologies).
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bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were incubated for 15 min at 25°C and approximately 5 µL of solution was loaded into Monolith NT Standard Capillaries (NanoTemper Technologies). Measurements were performed at 25°C using 20% LED and 20% IR-laser power with 5 s/30 s/5 s laser off/on/off times ...
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bioRxiv - Biochemistry 2021Quote: ... Measurements were conducted at 40% MST power and light-emitting diode (LED)/excitation power setting 20% in Premium Coated capillaries on the Monolith NT.115 at 25°C (Nanotemper Technologies). Each experiment was performed in at least three replicates ...
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bioRxiv - Biochemistry 2021Quote: ... The mixtures were loaded into capillaries and measured at ambient temperature by using 20% LED and medium MST power in a Monolith NT.115 system (NanoTemper Technologies). The data were analyzed using NanoTemper analysis software (v.1.2.101).
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bioRxiv - Molecular Biology 2021Quote: ... experiments were performed according to the NanoTemper technologies protocols in a Monolith NT.115 Pico (Pico Red / Nano Blue - NanoTemper Technologies). His-Cdc42 affinity for the RED-tris-NTA label was not optimal therefore Alexa Fluor 647– NHS dye was used ...
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bioRxiv - Biochemistry 2022Quote: Thermal-shift assays of SARS-CoV-2 Mpro and its H172Y mutant were carried out using the nanoDSF method as implemented in the Prometheus NT.48 (NanoTemper Technologies). The nanoDSF method is based on the autofluorescence of tryptophan (and tyrosine ...
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bioRxiv - Biochemistry 2022Quote: The thermal stability of SETX-HD WT and the R2136H variant was measured using the Prometheus NT.38 instrument (NanoTemper Technologies). NanoDSF grade capillaries were filled with protein samples at 1 mg/ml in protein dilution buffer (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2022Quote: ... the Kd (the dissociation constant) values between the effector and the RGA5 HMAs were measured using Monolith NT.115 (NanoTemper Technologies) with 30 % LED power and fitted with Nano Temper Analysis Software (Version 1.5.41) ...
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bioRxiv - Immunology 2022Quote: Nano differential scanning fluorimetry was used to assess the thermal stability of purified spike designs on a Prometheus NT.48 instrument (NanoTemper Technologies). Samples were diluted to 0.2 mg/mL with PBS and 20 μL of each sample was loaded into capillary tubes ...
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bioRxiv - Molecular Biology 2023Quote: ... All mixtures were prepared in 0.05% Tween 20 in PBS and the measurement was carried out in Monolith NT™ Standard capillaries (NanoTemper Technologies). Excitation power and MST power of Microscale Thermophoresis instrument (Monolith NT.115 ...
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bioRxiv - Biophysics 2023Quote: ... then loaded into standard treated capillaries for measurement with a Monolith NT.115 MST instrument (NanoTemper Technologies, South San Francisco, CA); measurements were taken at 37 °C with LED power ranges from 20-60% and medium MST power ...
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bioRxiv - Biophysics 2023Quote: ... The microscale thermophoresis (MST) traces of each sample were measured using a Nanotemper Monolith NT.115 (NanoTemper Technologies GmbH, Munich, Germany). The temperature-related fluorescence intensity change between the cold range (1 s before measurement started ...
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bioRxiv - Biochemistry 2023Quote: Microscale thermophoresis (MST) analysis was carried out to determine the binding affinity between hexon and hLF using Monolith NT.115 (Nanotemper Technologies). Hexon was labelled with AlexaFluor594 (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: The DNA binding affinities of HBb to the 80 bp (9, 37) and 80 bp-GC50 oligonucleotides were measured using the Monolith NT.115 instrument (NanoTemper Technologies). MST data were analyzed using the associated MO.Control software.
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bioRxiv - Cell Biology 2020Quote: ... his-tagged TLNRD1 was diluted to 100 nM and coupled with His-tag NT-647 dye (RED-tris-NTA NanoTemper, München, Germany) at room temperature for 30 min ...
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bioRxiv - Biophysics 2022Quote: Reactions were incubated for 15 minutes at room temperature in the dark and loaded into Monolith NT.115 Capillaries (NanoTemper Technologies GmbH). Microscale thermophoresis measurements were performed using a Monolith NT.115 (NanoTemper Technologies GmbH ...
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bioRxiv - Cancer Biology 2020Quote: MicroScale Thermophoresis experiments were performed according to the NanoTemper Technologies protocol in a Monolith NT.115 (red) instrument (NanoTemper Technologies, Munich, Germany). Binding was measured between EDA-GTP-Cy5 (Jena Bioscience GmbH ...
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bioRxiv - Biophysics 2021Quote: ... The microscale thermophoresis (MST) traces of each titration sample were measured using a Nanotemper Monolith NT.115 (NanoTemper Technologies GmbH, Munich, Germany). The temperature-related fluorescence intensity change was recorded for each sample and plotted against the concentration of CTLA-4 to derive the dissociation constant between anticalin and CTLA-4.
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bioRxiv - Cancer Biology 2020Quote: ... Microscale thermophoresis assays were performed on a NanoTemper ® Monolith NT.115 instrument with blue/red filters (NanoTemper Technologies GmbH, Munich, Germany). Samples were prepared in 20mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... and Spike RBD mixed with W25 (2 μM each) were prepared and loaded into Capillaries Tycho NT.6 (TY-C001, NanoTemper Technologies GmbH). The Tycho instrument applied a quick thermal ramp from 35°C to 95°C with a heating rate of 30°C/min and the unfolding of proteins were monitored through changes in the 350 nm / 330 nm fluorescence emission ratio ...
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bioRxiv - Molecular Biology 2020Quote: The binding of W25 and the structural integrity of Spike RBD was verified using a label-free thermal shift assay with Tycho NT.6 (Nanotemper Technologies GmbH) using intrinsic tryptophan and tyrosine fluorescence ...
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bioRxiv - Molecular Biology 2020Quote: ... between the W25 Nanobody and SARS-CoV-2 S1 of Spike RBD was measured by microscale thermophoresis (MST) using a Monolith NT.115PICO instrument (Nanotemper Technologies GmbH). Purified W25 was buffer exchanged into a PBS buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Thermal unfolding of G-actin (2 μM) and F-actin (2 μM) was performed with the Tycho NT.6 (Nanotemper, Munich, Germany) according to the manufacturer’s instructions in G-buffer (5 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The thermal stability of Conk-C3 derivatives was determined by differential scanning fluorimetry on a NanoTemper Tycho NT.6 (NanoTemper Technologies, Germany) with a back-reflection aggregation detection at a range from 35 to 95°C and with a heating rate of 20°C min-1 ...
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bioRxiv - Biophysics 2019Quote: Thermal protein denaturation stability analysis was performed via differential scanning fluorimetry (using intrinsic fluorescence of tryptophan residues) by slowly heating the sample in a Prometheus NT.48 scanning fluorimeter (NanoTemper Technologies, GmbH). Nucleoprotein samples in PBS buffer solution (at concentrations of 3 mg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... 10 ⌈L of 1 mg/mL ADCs were loaded into standard capillaries and mounted in a Prometheus NT.48 (Nanotemper Technologies, Germany) with excitation near UV ...
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bioRxiv - Microbiology 2019Quote: ... The mixtures were incubated for 15 min and added into glass capillaries and loaded into a Monolith NT.115 instrument (NanoTemper Technologies, Germany), the Kd values were determined using NanoTemper Analysis software (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... were incubated with 0 mM to 50 mM GMP/GDP-mannose at room temperature for 15 minutes before measuring the Tm using a Tycho NT.6 instrument (NanoTemper Technologies, Germany). All experiments were done in two biological repeats and three technical repeats ...
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bioRxiv - Biochemistry 2023Quote: In order to assess protein stability of all enzymes used in this study a thermal stability assay was performed using nanoDSF (Prometheus NT.48, NanoTemper Technologies GmbH). In this assay the enzymes were diluted to final concentrations of 0.5 mg/ml (USP1/UAF1 ...