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201 - 209 of 209 citations for C Reactive Protein Blocking Peptide since 2019

• Competition NMR for Detection of Hit/Lead Inhibitors of Protein-Protein Interactions

  Bogdan Musielak, et al., bioRxiv - Biophysics 2020

  Quote: ... PD-1 and its mutant were fluorescently labelled according the standard labeling protocol of the NanoTemper Protein Labeling Kit RED-NHS (L001-NanoTemper Technologies, Munich, Germany). In MST experiments ...
• Small-molecule inhibitors of the RNA m⁶A demethylase FTO potently support the survival of dopamine neurons

  Simona Selberg, et al., bioRxiv - Neuroscience 2021

  Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
• Novel RNA m⁶A methyltransferase METTL16 inhibitors

  Simona Selberg, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: Recombinant METTL16 MTD (residues 1-291) protein was labelled via His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies GmbH, Germany). The labelled protein was diluted in MST buffer (10 mM Na-phosphate buffer ...
• Bromodomain Factor 5 as a Target for Antileishmanial Drug Discovery

  Catherine N. Russell, et al., bioRxiv - Biochemistry 2023

  Quote: The BD5.1 protein was labelled with the Monolith His-Tag Labeling Kit (RED-tris-NTA 2nd Generation kit, NanoTemper Technologies, Catalog# MO-L018) according to the manufacturer’s protocol ...
• Listeria phages induce Cas9 degradation to protect lysogenic genomes

  Beatriz A. Osuna, et al., bioRxiv - Microbiology 2019

  Quote: The binding affinities of anti-CRISPR proteins to SpyCas9 were calculated using microscale thermophoresis (MST) on the Monolith NT.115 instrument (NanoTemper Technologies GmbH, Munich, Germany). For AcrIIA1/AcrIIA2b.3 with WT or mutant Cas9-gRNA complexes ...
• The K⁺ exchange antiporter 3 senses the chloroplast energy status to synchronize photosynthesis

  Michal Uflewski, et al., bioRxiv - Plant Biology 2022

  Quote: ... 50 nM of protein sample in PBS together with indicated concentrations of nucleotides were loaded into standard Monolith NT.115 capillaries (NanoTemper Technologies GmbH, Munich, Germany). MST measurement was performed using the Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
• The Strongest Protein Binder is Surprisingly Labile

  Alba Fernandez-Calvo, et al., bioRxiv - Biophysics 2024

  Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
• Systematic development of peptide inhibitors targeting the CXCL12/HMGB1 interaction

  Jacopo Sgrignani, et al., bioRxiv - Biochemistry 2020

  Quote: ... histidine tagged HMGB1 was labeled by the his-tag specific NT-647 dye (Monolith NTTM Protein Labelling Kit RED-NHS, NanoTemper® Technologies GmbH, Mu□nchen, Germany), for 30 minutes at room temperature ...
• Molecular basis of UHRF1 allosteric activation for synergistic histone modification binding by PI5P

  Papita Mandal, et al., bioRxiv - Biochemistry 2021

  Quote: Fluorophore-labeled protein was incubated with various ligands at room temperature for 15 minutes before measuring on Monolith NT.115 (NanoTemper, 80% LED power, 40% MST power). For competition assays ...