Labshake search
Citations for NanoTemper :
101 - 150 of 209 citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... g5Rp was first labeled using the Monolith NT Protein Labeling Kit RED-NHS (NanoTemper Technologies), and the labeled protein was then diluted to 20 nM with buffer containing 50 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: Thermal denaturation of ApoE proteins was studied by using a NanoDSF Prometheus instrument (NanoTemper, Germany). The protein samples at a concentration of 0.2 mg/ml were heated at a scan rate of 1 °C/min from 25 to 95 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used the Monolith Protein Labeling Kit RED-NHS 2nd Generation kit (NanoTemper MO- L011) to label ∼1 lysine per Fcy1 monomer with a fluorophore following the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2024Quote: We measured protein stability using nano-differential scanning fluorimetry on a Prometheus NT.48 (NanoTemper). After purification and his-tag cleavage ...
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bioRxiv - Biochemistry 2024Quote: ... FabF protein was labeled with the RED-MALEIMIDE or RDE-NHS dye (Nanotemper Technologies, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Experiments were run at 20% excitation and high MST power at 23.0°C on a Monolith NT.115Pico (NanoTemper). Each experimental compound was run in triplicate.
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and centrifuged at 14,000 rpm for 10 min at 4°C before loading into Monolith NT.115 premium capillaries (NanoTemper). Samples were loaded into the Monolith NT.115 device (NanoTemper ...
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bioRxiv - Molecular Biology 2020Quote: ... Intrinsic protein fluorescence at 330 nm and 350 nm was monitored between 15 and 90 °C in the Prometheus NT.48 instrument (Nanotemper), and the Tm values calculated within the accompanying software by taking the turning point of the first derivative of the F350:F330 ratio as a function of temperature.
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bioRxiv - Biochemistry 2019Quote: ... was monitored during unfolding over a linear temperature gradient (35-95 °C) over 3 minutes on a Tycho NT.6 instrument (NanoTemper). The temperature at which a transition occurs in a plot of the first derivative of the fluorescence ratio (350/330 nm) ...
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bioRxiv - Biophysics 2020Quote: Thermal unfolding of BG505-SOSIP was determined with a heating ramp of 1°C/min and 20% sensitivity setting using high-sensitivity capillaries (NanoTemper Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Equilibrated mixtures containing 5µM of PdtaS-EGFP and varying concentrations of c-di-GMP were loading onto hydrophobic capillaries (NanoTemper Technologies). The laser on and off times were set at 30s and 5s respectively ...
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bioRxiv - Biophysics 2020Quote: Proteins mixed to a final concentration of 30 μM in presence of 200 μM PIP2 and incubated overnight at 4 °C were used to fill two standard grade NanoDSF capillaries (Nanotemper) and loaded into a Prometheus NT.48 device (Nanotemper ...
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bioRxiv - Biochemistry 2022Quote: ... Then the mixture was incubated overnight at 16 °C followed by centrifugation at 15,000 g for 15 min before loaded into the Premium Capillaries (NanoTemper Technologies). The thermophoresis analysis ...
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bioRxiv - Biochemistry 2024Quote: ... Measurements were performed in triplets at 25 °C and at medium MST power in Monolith NT.115 Capillaries (NanoTemper Technologies). The raw data were analyzed using the MO.Affinity Analysis software (NanoTemper Technologies).
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bioRxiv - Biophysics 2019Quote: The stability of the different proteins was measured by nanoDSF (Prometheus NT.48, NanoTemper Technologies, GmbH). The proteins were first dialyzed against 1 l of gel filtration buffer supplemented with 5 mM EDTA overnight at 4 °C and subsequently 2x against 1 l of gel filtration buffer without EDTA overnight at 4 °C ...
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bioRxiv - Pathology 2021Quote: Spike RBD proteins were labeled using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies) following manufacturer protocol at 2:1 protein to dye ratio ...
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bioRxiv - Molecular Biology 2022Quote: The thermal stability of the proteins was determined using Prometheus NT.48 nanoDSF instrument from NanoTemper Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μM PfCoSP was labeled using NanoTemper’s Protein Labeling Kit RED-NHS (L001, NanoTemper technologies, Germany). The concentration of labeled PfCoSP protein was kept constant at a concentration of 90 nM ...
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bioRxiv - Cell Biology 2023Quote: His-tagged MLCK proteins were labeled using a His-Tag Labeling Kit RED-tris-NTA (NanoTemper). Microscale thermophoresis was performed using a Monolith NT.115 (NanoTemper) ...
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bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were incubated for 15 min at 25°C and approximately 5 µL of solution was loaded into Monolith NT Standard Capillaries (NanoTemper Technologies). Measurements were performed at 25°C using 20% LED and 20% IR-laser power with 5 s/30 s/5 s laser off/on/off times ...
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bioRxiv - Biochemistry 2021Quote: ... Measurements were conducted at 40% MST power and light-emitting diode (LED)/excitation power setting 20% in Premium Coated capillaries on the Monolith NT.115 at 25°C (Nanotemper Technologies). Each experiment was performed in at least three replicates ...
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bioRxiv - Cell Biology 2023Quote: ... Measurements were performed at 22 °C using medium MST power and 100% excitation power settings on the MO.control data collection software (NanoTemper Technologies GmbH). The data were analyzed and fit using the MO.Affinity Analysis software (NanoTemper Technologies GmbH ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The protein binding was measured by the microscale thermophoresis technique on a Monolith NT.115 instrument (Nanotemper). Protein (10 nM ...
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bioRxiv - Cell Biology 2021Quote: ... The RNA-protein mixtures were analyzed in premium capillaries using the Monolith NT.115T MST (NanoTemper Technologies). Measurements were carried out at 25 °C ...
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bioRxiv - Biochemistry 2022Quote: NanoDSF and light scattering of purified proteins were measured with a Prometheus NT.48 (Nanotemper, Munich, Germany), equipped with back-reflection optics or with a Prometheus Panta (Nanotemper ...
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bioRxiv - Biochemistry 2022Quote: ... Then the proteins were loaded onto Prometheus NT.48 nanoDSF Grade Standard Capillaries (PR-C002, NanoTemper Technologies), the fluorescence signal was recorded under a temperature gradient ranging from 25 to 90°C (incremental steps of 0.5°C min-1) ...
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bioRxiv - Biochemistry 2022Quote: ... The full-length SLC19A1 protein was fluorescently labelled with a Red-NHS 2nd Generation kit (NanoTemper Technologies) in Buffer D (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: Protein thermal stability was determined by differential scanning fluorimetry (nanoDSF) on Prometheus NT.48 instrument (Nanotemper Technologies) in nanoDSF grade standard capillaries filled with the samples ...
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bioRxiv - Cell Biology 2023Quote: ... NRP-1 b1b2 domains were labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies, Munich, Germany). The interaction of NRP-1 structural variants with FOL-peptides was measured using a Monolith NT.115 [NT.115Pico/NT.LabelFree] instrument (NanoTemper Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The Csy complexes were fluorescence-labeled using the Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies) at 10 μM ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Protein and compound mixtures were loaded into standard treated capillaries (MO-K022, NanoTemper Technologies GmbH, Munich, Germany). Measurements were performed on a Monolith NT.115 with blue/red filters (NanoTemper Technologies GmbH ...
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bioRxiv - Biochemistry 2024Quote: Thermal stability of proteins was analyzed using nanoscale differential scanning fluorimetry with a Prometheus NT.48 (NanoTemper) device ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The quality of the protein was determined using Differential Scanning Fluorimetry (Prometheus NT.48, NanoTemper Technologies GmbH). The uniform labelling of KIX domain was confirmed by MALDI-TOF MS.
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bioRxiv - Biochemistry 2021Quote: Microscale thermophoresis measurements were performed at 25°C using Monolith NT™ premium capillaries and the Monolith NT.115 device with a green filter (NanoTemper, Germany). Power of LED and IR-laser sources are indicated in figure legends ...
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bioRxiv - Biochemistry 2021Quote: Protein thermal stability was measured in a label-free fluorescence assay using a Prometheus NT.48 (NanoTemper, Germany). Briefly ...
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bioRxiv - Biochemistry 2020Quote: His6-GlnK1 was purified and labeled using Monolith Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper, Munich, Germany) (DOL ...
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bioRxiv - Microbiology 2021Quote: ... TgPRP4K protein was labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies). The labeled TgPRP4K protein was adjusted to 100 nM with a buffer containing 30 mM Hepes (PH 7.5) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The protein (200 nM) was fluorescently labeled using a RED-tris-NTA 2nd generation dye (NanoTemper Technologies GmbH) and a 1:1 dye/protein molar ratio in the reaction buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... between protein:compound sample and a protein only sample at 1.5-2-5 sec is calculated and plotted through MO.Affinity analysis v2.3 (NanoTemper Technologies) and GraphPad Prism 8.0.0 (GraphPad Software ...
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bioRxiv - Biochemistry 2022Quote: ... Labeling of RIP2K1-300 was performed with Monolith Protein labeling kit RED-NHS 2nd generation (MO-L011, Nanotemper) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Thermal melting profiles of proteins were measured by differential scanning fluorimetry on a Prometheus NT.48 instrument (NanoTemper). Protein samples (0.1 mg/mL ...
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bioRxiv - Immunology 2023Quote: Thermal melting profiles of proteins were measured by differential scanning fluorimetry on a Prometheus NT.48 instrument (NanoTemper). Protein samples (0.1 mg/mL ...
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bioRxiv - Biochemistry 2024Quote: Wt and P323L variants of nsp12 were labeled with NHS-fluorescent label utilizing the Protein labelling kit (Nanotemper). Labeled proteins were then diluted to either 105nM for protein-protein interactions or 52.5 nM for complex formation and utilized as a target molecule ...
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bioRxiv - Cell Biology 2024Quote: Purified protein was labeled using the His-Tag labeling Kit RED-tris-NTA 2nd Generation (Nanotemper, MO-L018) following the manufacturer’s directions ...
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bioRxiv - Biochemistry 2023Quote: ... and centrifuged at 20k ×g at 4 °C for 5 min prior to loading into standard-coated capillary tubes (NanoTemper, GmbH, Munich, Germany). Microscale thermophoresis data were collected on a Monolith NT.115 (NanoTemper ...
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bioRxiv - Molecular Biology 2021Quote: ... The onset of scattering and melting temperatures based on the change of intrinsic protein fluorescence (ratio 350 nm/330 nm) were calculated in PR.ThermControl software (NanoTemper).
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bioRxiv - Biochemistry 2019Quote: The thermal stability of wild-type and variant RmtC proteins was assessed using a Tycho NT.6 instrument (NanoTemper) to ensure protein quality between different preparations of proteins and before/ after freezing ...
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bioRxiv - Biophysics 2020Quote: hGH was labelled with NT-647-NHS88 using the Monolith NT™ Protein Labelling Kit RED-NHS (NanoTemper Technologies) for 1 h at room temperature with NT-647-NHS at a molar ratio of 1:3 in labelling buffer following the protocol ...
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bioRxiv - Biochemistry 2020Quote: Purified His6-GlnA1 was fluorescently labeled using the Monolith Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper, Munich, Germany) with buffer B (50 mM HEPES ...
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bioRxiv - Cancer Biology 2019Quote: ... was labeled for microscale thermophoresis (MST) using the Monolith NT Protein RED-NHS Labeling Kit (NanoTemper Technologies, München, Germany) according to the instructions of the manufacturer ...