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Citations for NanoTemper :
151 - 170 of 170 citations for Saporin Chicken Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
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bioRxiv - Biophysics 2022Quote: Recombinantly purified protein constructs were labelled with RED-tris NTA dye (NanoTemper Technologies GmbH) in PBS to a concentration of 100 nM ...
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bioRxiv - Molecular Biology 2020Quote: Purified SecB was randomly labelled using an NT-647-NHS labelling kit (Nanotemper Technologies). 160 nM NT-647-labelled SecB was incubated with the indicated concentrations of unlabelled AscA or AscAΔMBD in buffer (20 mM potassium HEPES [pH 7.5] ...
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bioRxiv - Neuroscience 2023Quote: ... into 50 nM of fluorescently labeled Munc13-1 constructs in a premium-coated glass capillary using a Monolith NT.115 (Nanotemper Technologies, San Francisco, CA).
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bioRxiv - Biophysics 2024Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
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bioRxiv - Microbiology 2022Quote: Thermal stabilities of purified CydDC variants were investigated with a Prometheus NT.48 instrument (Nanotemper). Excitation at 280 nm (20 nm bandwidth ...
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bioRxiv - Biochemistry 2020Quote: ... histidine tagged HMGB1 was labeled by the his-tag specific NT-647 dye (Monolith NTTM Protein Labelling Kit RED-NHS, NanoTemper® Technologies GmbH, Mu□nchen, Germany), for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: Fluorophore-labeled protein was incubated with various ligands at room temperature for 15 minutes before measuring on Monolith NT.115 (NanoTemper, 80% LED power, 40% MST power). For competition assays ...
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bioRxiv - Biochemistry 2022Quote: NanoDSF and light scattering of purified proteins were measured with a Prometheus NT.48 (Nanotemper, Munich, Germany), equipped with back-reflection optics or with a Prometheus Panta (Nanotemper ...
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bioRxiv - Biochemistry 2020Quote: ... Purified DVD-actin was labelled using Monolith NT protein labelling kit RED-NHS (Amine Reactive) dye (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescent NbSyt1 was incubated with different dilutions of purified Syt-1(97-421) using Premium Coated Capillaries (NanoTemper). For operation of the instrument and evaluation of affinity data ...
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bioRxiv - Cancer Biology 2023Quote: ... Aliquots (10 μl) of purified mTORC1_WT or mTORC1_EWEDdup variant were loaded into the Prometheus capillaries (Cat. PR-C002, NanoTemper Technologies), and the fluorescence intensity at 330 nm and 350 nm was recorded as a function of temperature from 10 °C to 90 °C ...
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bioRxiv - Microbiology 2020Quote: NanoDSF Systems were conducted to measure the thermostability and aggregation of purified protein using Prometheus NT.48 instrument (NanoTemper Technologies). A 10 μL aliquot of sample diluted to a concentration of 5 mg/mL were applied to quartz capillary cassette and placed into card slot ...
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bioRxiv - Developmental Biology 2022Quote: Binding assays of purified NHR23 or strep-NHR85 was measured using a Monolith NT.115 Pico running MO Control version 1.6 (NanoTemper Technologies). Assays were performed in 100 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: Binding of either guanine or xanthine by purified PurTCp was measured by microscale thermophoresis (MST) using the Monolith NT.LabelFree and Monolith NT.115 (NanoTemper Technologies, Germany) to detect the thermophoretic signals of native tryptophan and tagged fluorescence in PurTCp ...
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bioRxiv - Cancer Biology 2021Quote: Binding of purified recombinant KIX with fluorescein isothiocyanate (FITC)-conjugated peptides was measured by microscale thermophoresis using the Monolith NT.115 (NanoTemper Technologies). Assays were conducted in 50 mM sodium phosphate ...
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bioRxiv - Immunology 2022Quote: Thermal unfolding of purified HAssF in PBS was measured by nano differential scanning fluorometry (nDSF) using Prometheus NT.48 instrument (NanoTemper technologies). Thermal unfolding curves are plotted slope (Δ(F350 nm/F330 nm)/ΔT ...
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bioRxiv - Immunology 2022Quote: Nano differential scanning fluorimetry was used to assess the thermal stability of purified spike designs on a Prometheus NT.48 instrument (NanoTemper Technologies). Samples were diluted to 0.2 mg/mL with PBS and 20 μL of each sample was loaded into capillary tubes ...
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bioRxiv - Biochemistry 2020Quote: ... was applied to a dilution series of purified His6-GlnA1 ranging from nM to µM concentrations and measured using standard treated capillaries (NanoTemper, Munich, Germany), 100% Excitation Power and medium MST-Power.
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bioRxiv - Plant Biology 2022Quote: The purified protein in PBS at a concentration of ∼16 µM was loaded into capillaries (nanoDSF grade standard, NanoTemper GmbH, Munich, Germany) and a melting curve was determined by using the nanoDSF Prometheus NT.48 instrument (NanoTemper GmbH ...