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Citations for NanoTemper :
51 - 100 of 170 citations for Saporin Chicken Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Affinity measurements were conducted in Premium Capillaries Monolith NT.115 (MO-K025, NanoTemper Technologies GmbH) and repeated three times ...
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bioRxiv - Biochemistry 2022Quote: Binding affinity analysis by microscale thermophoresis was performed using the Monolith NT instrument (Nanotemper Technologies). All reactions (in triplicates ...
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bioRxiv - Cancer Biology 2023Quote: Binding affinities were estimated by microscale thermophoresis using a Monolith NT.115 device (NanoTemper, Germany). We measured the thermophoretic movements of labeled molecules within a temperature gradient ...
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bioRxiv - Molecular Biology 2024Quote: ... The Kd values and Hill coefficients (nH) were calculated using MO Affinity Analysis software (NanoTemper). All data were statistically analyzed and graphed using GraphPad Prism software (v5.0).
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bioRxiv - Biochemistry 2020Quote: ... the binding affinity between SpaKC and SpaAleader was measured using Monolith NT.115 Pico (Nanotemper Technologies). SpaKC was fluorescently labelled by the labeling kit (Monolith Protein Labeling Kit RED-NHS 2nd Generation (Amine Reactive) ...
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bioRxiv - Molecular Biology 2023Quote: Binding affinity comparisons via microscale thermophoresis were performed using the Monolith NT.115 instrument (NanoTemper Technologies). The CTD polypeptides (CTD ...
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bioRxiv - Biochemistry 2023Quote: ... binding affinities were determined via microscale thermophoresis on a Monolith NT.115 instrument (NanoTemper Technologies, Germany). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... a 16-point affinity measurement was performed in duplicates using the Dianthus (NanoTemper Technologies GmbH, Germany) instrument ...
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bioRxiv - Molecular Biology 2024Quote: Binding affinity comparisons via microscale thermophoresis were performed using the Monolith NT.115 instrument (NanoTemper Technologies). The CTD polypeptides (CTD ...
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bioRxiv - Microbiology 2019Quote: ... Hexahistidine-tagged ALIX protein was labeled by NTA-dye (NanoTemper Technologies) using manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The binding affinity measurements were carried out using the modified manufacturer’s protocol (Monolith NT.115pico, NanoTemper Technologies) in the Center for Macromolecular Interactions in Harvard Medical School (Boston ...
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bioRxiv - Neuroscience 2023Quote: The affinity of the NbSyt1 was measured by microscale thermophoresis using the device NT.115Pico Monolith (NanoTemper). NbSyt1 was labelled with Alexa647 (as described above ...
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bioRxiv - Biochemistry 2022Quote: The affinity of SLC19A1 for TPP was determined using a NanoTemper Monolith NT.115 instrument (NanoTemper Technologies). The full-length SLC19A1 protein was fluorescently labelled with a Red-NHS 2nd Generation kit (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were labeled with a RED-NHS Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were labeled with a RED-NHS Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... KD was determined according to the law of mass action using the program MO Affinity Analysis (NanoTemper Technologies) and results were plotted using Prism (Graphpad).
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bioRxiv - Biochemistry 2021Quote: ... Thermophoresis data were analyzed when fluorescence signals lies within the 10% acceptance range using MO Affinity Analysis software V2.1.2 (Nanotemper). Data were plotted using GraphPad Prism 4.0 software and fitted with nonlinear equation.
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bioRxiv - Microbiology 2021Quote: Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: NfrB414-745 was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions in the supplied labeling buffer applying a concentration of 20 μM protein at room temperature for 30 min in the dark ...
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bioRxiv - Biophysics 2021Quote: Thermal stability and binding affinity were determined using nanoscale differential scanning fluorimetry on a Prometheus NT.48 (NanoTemper Technologies). Capillaries were filled with 10 μL of respective samples in duplicates and loaded into the reading chamber ...
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bioRxiv - Cancer Biology 2020Quote: ... Data analyses and the dissociation constants (Kd) calculation were performed using MO Affinity Analysis software (NanoTemper Technologies, Munich, Germany). Binding of radiolabeled GTP was measured by means of the SPA ...
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bioRxiv - Molecular Biology 2021Quote: TCNB4 was covalently labeled with Red-NHS using the Monolith NT kit (Nanotemper technologies) and used in the cellular binding and uptake studies ...
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... were labeled with the amine-reactive dye NT-647 Red-NHS (NanoTemper, Munich, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... containing 6.25 μM of His-tagged PyrE labeled with RED dye (Nanotemper Technologies, USA), with an equal volume of the respective ligand solution ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled using the Monolith His-Tag Labeling Kit Red-tris-NTA (NanoTemper Technologies) and diluted in MST buffer (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2020Quote: The binding affinities of compound 20 to Sc-Dyn-lysoMut was measured using Monolith NT.115 (NanoTemper Technologies, Munich, Germany). Sc-Dyn-lysoMut was labeled with the Monolith NT Protein Labeling Kit RED-NHS ...
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bioRxiv - Biophysics 2020Quote: The binding affinity of spike to Sb23 was measured by microscale thermophoresis using the MST NT.115 device (NanoTemper Technologies). Sb23 was labelled via the His6-tag with RED-tris-NTA dye (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2019Quote: Apparent Kd values were computed fitting the compound concentration-dependent changes in normalized fluorescence (Fnorm) by the MO Affinity Analysis software provided by Nanotemper. Final results were obtained averaging four independent experiments.
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bioRxiv - Molecular Biology 2023Quote: The affinity between RNA and protein and between proteins was measured by MST on a Monolith NT.115 (NanoTemper, Germany). Replicates represent individual measurements performed on different days using proteins from the same prep ...
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bioRxiv - Microbiology 2022Quote: ... Normalization of the fluorescence signal and fitting to the Hill equation were performed using the software MO Affinity Analysis v2.3 (NanoTemper Technologies). For each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... Nanobodies were labeled using the lysine reactive Monolith NT Protein Labeling kit RED-NHS (NanoTemper). As TCNB11 has a lysine residue in CDR2 ...
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bioRxiv - Biophysics 2022Quote: ... was labeled with RED-NHS (2nd Generation) dye using the Monolith Protein Labeling Kit (NanoTemper Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... PrgU-His was labeled with the RED 2nd generation His-tag labeling kit (NanoTemper Technologies) according to the manufacturer’s instructions with a final concentration in the assay of 50 nM (molar dye ...
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bioRxiv - Biochemistry 2021Quote: ... g5Rp was first labeled using the Monolith NT Protein Labeling Kit RED-NHS (NanoTemper Technologies), and the labeled protein was then diluted to 20 nM with buffer containing 50 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labelled protein was added to a serial dilution of unlabeled DAG ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies). Labeled proteins were added to a serial dilution of unlabeled DAG or liposomes prepared in binding buffer (20 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... EED and JMJD3 proteins were labeled with Protein Labeling kit RED-NHS (NanoTemper, MO-L001). At least three independent experiments were performed for each binding reaction.
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bioRxiv - Cell Biology 2023Quote: His6-CTxA1 was labeled using Monolith NT His-Tag Labeling Kit RED-tris-NTA (NanoTemper). CTxB labeled with Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... FabF protein was labeled with the RED-MALEIMIDE or RDE-NHS dye (Nanotemper Technologies, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: The data was analyzed and the Ktet (Eq. 1) or EC50 (Eq. 2) calculated using the MO affinity analysis software (NanoTemper, Germany), considering a 1:1 model.
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bioRxiv - Microbiology 2019Quote: ... The binding affinities were determined to fit the data points to a quadratic equation (equation 1) using non-linear regression using MO Affinity software (NanoTemper Technologies), Prism (GraphPad Inc ...
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bioRxiv - Biophysics 2020Quote: ... The assay was performed in duplicate and the values reported were generated through the usage of MO Affinity Analysis software (NanoTemper Technologies).
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bioRxiv - Biochemistry 2023Quote: ... The best data quality was obtained at 90% MST power which was thus used for quantifying the binding affinities using the MO.Affinity Analysis software (NanoTemper Technologies, Germany).
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bioRxiv - Biochemistry 2023Quote: Microscale thermophoresis (MST) analysis was carried out to determine the binding affinity between hexon and hLF using Monolith NT.115 (Nanotemper Technologies). Hexon was labelled with AlexaFluor594 (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: The binding affinity of each recombinant TrK13 protein for heme was evaluated by MST analysis using the Monolith NT.115 instrument (NanoTemper Technologies). The recombinant TrK13 proteins (50 nM ...
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bioRxiv - Cancer Biology 2019Quote: KDM4B was labeled with a cysteine reactive fluorescent dye (Protein Labeling Kit RED-MALEIMIDE, Nanotemper GmbH). A serial dilution of titrant was prepared in MST buffer containing 10 mM HEPES (pH 7.5) ...
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bioRxiv - Pathology 2021Quote: Spike RBD proteins were labeled using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies) following manufacturer protocol at 2:1 protein to dye ratio ...
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bioRxiv - Microbiology 2020Quote: ... Each protein (10 μM) was labeled with NT-647-NHS reactive dye (30 μM) (Nanotemper, Germany), which reacted efficiently with the primary amines of the proteins to form a stable dye protein conjugate ...
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bioRxiv - Biochemistry 2019Quote: ... was labeled with Monolith His-Tag Labeling Kit RED-tris-NTA kit (NanoTemper Technologies, München, Germany). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μM PfCoSP was labeled using NanoTemper’s Protein Labeling Kit RED-NHS (L001, NanoTemper technologies, Germany). The concentration of labeled PfCoSP protein was kept constant at a concentration of 90 nM ...