Labshake search
Citations for NanoTemper :
101 - 150 of 177 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... IS621 recombinase was labeled for MST using the RED-MALEIMIDE 2nd Generation cysteine reactive kit (NanoTemper technologies) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The Csy complexes were fluorescence-labeled using the Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies) at 10 μM ...
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bioRxiv - Biochemistry 2022Quote: ... The full-length SLC19A1 protein was fluorescently labelled with a Red-NHS 2nd Generation kit (NanoTemper Technologies) in Buffer D (25 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... were fluorescently labeled using His-Tag Labeling Kit RED-Tris-NTA 2nd Generation (NanoTemper, Cat# MO-L018). The proteins (at fixed concentration ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were fluorescently labeled using the RED-Maleimide 2nd Generation Protein Labeling Kit (NanoTemper Technologies GmbH, Germany) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... NRP-1 b1b2 domains were labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies, Munich, Germany). The interaction of NRP-1 structural variants with FOL-peptides was measured using a Monolith NT.115 [NT.115Pico/NT.LabelFree] instrument (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2020Quote: His6-GlnK1 was purified and labeled using Monolith Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper, Munich, Germany) (DOL ...
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bioRxiv - Microbiology 2021Quote: ... TgPRP4K protein was labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies). The labeled TgPRP4K protein was adjusted to 100 nM with a buffer containing 30 mM Hepes (PH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... S1-His or RBD-His were labelled using Monolith NTTM His-Tag Labeling Kit RED-tris-NTA (Nanotemper) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Purified DVD-actin was labelled using Monolith NT protein labelling kit RED-NHS (Amine Reactive) dye (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... PGLRs were labelled with monolith protein labelling kit blue NHS amine reactive (Lys, NanoTemper, catalog no. MO-L003) and conserved in MST buffer (50 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... Labeling of RIP2K1-300 was performed with Monolith Protein labeling kit RED-NHS 2nd generation (MO-L011, Nanotemper) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... followed by labeling with 30 μM Cysteine reactive dye (Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper), and incubated in dark at Room Temperature (RT ...
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bioRxiv - Plant Biology 2022Quote: ... The N-terminal His-tag was labelled with the RED-tris-NTA kit (NanoTemper Technologies GmbH, Munich, Germany). After 10 minutes incubation on ice ...
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bioRxiv - Biochemistry 2024Quote: Wt and P323L variants of nsp12 were labeled with NHS-fluorescent label utilizing the Protein labelling kit (Nanotemper). Labeled proteins were then diluted to either 105nM for protein-protein interactions or 52.5 nM for complex formation and utilized as a target molecule ...
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bioRxiv - Cell Biology 2024Quote: Purified protein was labeled using the His-Tag labeling Kit RED-tris-NTA 2nd Generation (Nanotemper, MO-L018) following the manufacturer’s directions ...
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bioRxiv - Biochemistry 2024Quote: ... followed by labeling with cysteine-reactive dye (30 mM; Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper) and incubated for 30 min at room temperature (RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Sla1:355-414 and Sla2cc were labelled using the 2nd Generation RED-NHS labelling kit (Nanotemper, Munich, Germany). The reaction was done in SEC buffer ...
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bioRxiv - Genetics 2024Quote: ... purified proteins were labeled with His-Tag labeling kit RED-tris-NTA following the manufacturer’s instructions (Nanotemper technologies), and 50 ng of labelled proteins were mixed with serial dilution of the lipids of interest and loaded into MST standard capillaries ...
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bioRxiv - Biophysics 2020Quote: hGH was labelled with NT-647-NHS88 using the Monolith NT™ Protein Labelling Kit RED-NHS (NanoTemper Technologies) for 1 h at room temperature with NT-647-NHS at a molar ratio of 1:3 in labelling buffer following the protocol ...
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bioRxiv - Biochemistry 2020Quote: Purified His6-GlnA1 was fluorescently labeled using the Monolith Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper, Munich, Germany) with buffer B (50 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... MBP-PDF2(START)L467P were labeled in 50 mM sodium phosphate buffer (pH 7.4) supplemented with 500 mM NaCl using Monolith Protein Labeling kit RED-MALEIMIDE (NanoTemper) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... W25 was fluorescently labeled with the Monolith Protein Labeling Kit RED – NHS 2nd Generation (MO-L011, NanoTemper Technologies GmbH) following the protocol established in the manual ...
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bioRxiv - Cell Biology 2021Quote: Recombinant His-tagged proteins were labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper, MO-L008). In all experiments ...
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bioRxiv - Molecular Biology 2022Quote: SiaQM in amphipol was labelled at a ratio of 0.8 dye / 1 protein following the protocol of the RED-NHS 2nd generation labelling kit (NanoTemper). Labelled SiaQM was held constant at 20 nM and SiaP was titrated at 16 concentrations starting at 2,900 μM and serially diluting 2-fold to 90 nM ...
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bioRxiv - Biophysics 2022Quote: 6.6 µM GyrA14 (in 1X PBS) was labeled using the RED-NHS Monolith™ Protein Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The purified PDZ domains were covalently labelled using a fluorescent dye reactive on amine following the manufacture’s protocol (Protein Labelling Kit RED-NHS, Nanotemper). A serial dilution of the WT and the P71L mutant of the E protein PBM peptide was prepared in the buffer containing PBS tween 20 0.05% ...
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bioRxiv - Biophysics 2020Quote: ... zRETECD P291-Q296;AAG was labelled with NHS-RED 2nd generation dye (Amine Reactive) using the labelling kit (Nanotemper), and the procedure was carried out as above with zRETECD P291-Q296;AAG -NHS-RED (50 nM ...
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bioRxiv - Systems Biology 2023Quote: ... NSP10 protein was fluorescently labeled using N-Hydroxysuccinimide (NHS)-ester fluorophores according to the manufacturer’s instructions (Protein Labeling Kit RED-NHS 2nd Generation, Nanotemper). Labeling was performed in the NSP16 protein storage buffer (20 mM HEPES pH 7.5 and 0.3 M NaCl) ...
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bioRxiv - Neuroscience 2023Quote: ... was labelled via His-tag with Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies GmbH). The labelled complex was diluted in MST buffer (10 mM Na-phosphate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were labelled in NHS labelling buffer using the Red NHS 2nd generation amine labelling kit (Nanotemper, MO-L011), then buffer exchanged into 10 mM potassium phosphate buffer ...
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bioRxiv - Biochemistry 2024Quote: ... 5% glycerol with 0.1% Tween20 was labelled with Monolith His-Tag Labelling Kit RED-tris-NTA 2nd Generation (NanoTemper) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... concentrated to 1 µM and lysine labeling was performed using the Red-NHS 2nd Generation Protein Labeling Kit (Nanotemper) according to manufacturer instructions ...
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bioRxiv - Genetics 2024Quote: Recombinant IRF6 protein was labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA (Nanotemper Technologies, MO-L018) according to the manufacturer’s protocol at a 1:2 dye:protein ratio ...
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bioRxiv - Biochemistry 2020Quote: ... This FSS-PscN solution was then labeled with the Monolith Protein Labeling Kit RED-NHS (MO-L001) following the manufacturer instruction (Nanotemper) and diluted in the purification binding buffer (see above ...
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bioRxiv - Microbiology 2022Quote: The purified HBsAg was purchased from ProSpec (hbs-872-c) and was labeled by RED-NHS dye (monolith protein labeling kit, MO-L011, NanoTemper) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: C-terminally His6-tagged proteins were labeled using the Monolith His-tag labeling kit RED-tris-NTA (Nanotemper #MO-L008) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... His-Cdc42 was labeled following the instructions of the MO-L001 Monolith NT Protein Labeling Kit RED – NHS (NanoTemper Technologies). Labelled protein concentration in the binding reactions was 10 nM while compounds concentration was either 50 or 100 μM ...
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bioRxiv - Biochemistry 2022Quote: The amine residues of purified KsgA were fluorescently labeled with NHS red using the Protein Labeling Kit RED-NHS 2nd Generation (Cat # MO-L011 Nanotemper). The labeling reaction was performed according to the manufacturer’s protocol by mixing KsgA at a final concentration of 20 μM with a 3-fold molar excess of dye at room temperature for 30 minutes in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... The TPR-CHAT and its mutants were fluorescence-labeled using the Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies) at 10 μM ...
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bioRxiv - Neuroscience 2024Quote: We followed the manufacturer’s instructions to label APOE3 and APOE4 using the Monolith protein labeling kit RED-NHS 2nd generation (NanoTemper Technologies). In summary ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper) according to the manufacturers’ recommendation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CK1γ2 was prepared at concentrations from 0.305 to 10000□nM and fluorescence dye from the Monolith His-tag Labeling kit RED-tris-NTA (NanoTemper Technologies) were added to concentrations of 80□nM and 5□nM ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ColG-variants were labeled using the Monolith NT™ Protein Labeling Kit RED-NHS 2nd Generation Amine reactive (NanoTemper, Germany) and the yield of labelled protein and the degree of labelling were determined according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2023Quote: ... 650 nm fluorescent dye using the Monolith NT RED-NHS lysine labeling kit according to manufacturer’s protocol (NanoTemper, Munich, Germany). RED labeled untagged sP36 at 100 nM and His6-GlnK1 in 16 different concentrations ranging from 7.5 µM to 0.23 nM or 10 nM of sP36-RED and AmtB1-His6 in 16 dilutions ranging from 12.6 µM to 0.38 nM (all concentrations based on monomeric molecular mass ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 µL of 100 nM solution of his-tagged protein was labeled with RED-tris-NTA 2nd Generation dye (Monolith HIS-tag labeling Kit, NanoTemper). Protein-protein complexes were obtained by mixing dye-labeled IFIT protein (final concentration 50 nM ...
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bioRxiv - Biochemistry 2024Quote: Site-specific labelling of His-tagged BRISC was performed using a RED-tris-NTA 2nd generation labelling kit (NanoTemper Technologies) in buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... Purified recombinant PTPN2 protein (20 μM) was labeled according to the protein labeling kit RED-NHS (Nanotemper, Cat. No. L001). The K73 compound was diluted into 16 concentration gradients with 1xPBS-P using the fold-and-half dilution method ...
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bioRxiv - Biophysics 2024Quote: ... were resuspended in PBS-0.05%T buffer provided by Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (Nanotemper, Germany) to stock concentrations of 5 mM and stored at -20 °C.
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bioRxiv - Immunology 2022Quote: ... Germany) and MST-Red-MIF was prepared using the Monolith Protein Labeling Kit RED-NHS 2nd Generation from NanoTemper (Munich, Germany), following the manufacturers’ instructions ...