Labshake search
Citations for NanoTemper :
1 - 50 of 177 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: The binding affinity of each recombinant TrK13 protein for heme was evaluated by MST analysis using the Monolith NT.115 instrument (NanoTemper Technologies). The recombinant TrK13 proteins (50 nM ...
-
bioRxiv - Molecular Biology 2022Quote: Binding assays were performed by electrophoretic mobility shift assay (EMSA) and Microscale thermophoresis (MST, NanoTemper Munich). Dilution series (1.5 fold ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Binding assays were performed using NanoTemper NT.LabelFree instrument (NanoTemper Technologies). All experiments were carried out at room temperature in hydrophobic capillaries with 20% light-emitting diode power (fluorescence lamp intensity ...
-
bioRxiv - Plant Biology 2023Quote: ... MST assays were performed according to protocols provided by NanoTemper Technologies (20% LED power and 40% MST power) ...
-
bioRxiv - Biochemistry 2024Quote: DSF assays were performed using Tycho NT.6 (NanoTemper Technologies). RBDs were diluted to approximatively 1 mg/mL ...
-
bioRxiv - Molecular Biology 2024Quote: MST assays were performed with a Monolith NT.115 (NanoTemper) using the Nano BLUE mode ...
-
bioRxiv - Biochemistry 2024Quote: ... Binding assays were performed on a NT.LabelFree Monolith instrument (NanoTemper) using Monolith NT.LabelFree capillaries from NanoTemper GmbH (Germany) ...
-
bioRxiv - Biochemistry 2024Quote: Thermal shift assays were performed on a Prometheus Panta (Nanotemper) instrument ...
-
bioRxiv - Plant Biology 2020Quote: MST assays were carried out using a Monolith NT.115 (NanoTemper) machine at the Chemical Genomics Facility of Purdue University ...
-
bioRxiv - Immunology 2023Quote: Thermal shift assay was performed using Tycho NT.6 (NanoTemper Technologies). Envelope ectodomain SOSIPs were diluted (0.15LmgLml−1 ...
-
bioRxiv - Biophysics 2023Quote: Thermal shift assay was performed using Tycho NT.6 (NanoTemper Technologies). Envelope ectodomain SOSIPs were diluted (0.15 mg ml−1 ...
-
bioRxiv - Biophysics 2024Quote: ... The thermostability assay was performed using Tycho NT.6 (NanoTemper Technologies). Protein samples were heated from 35 °C to 95 °C at 30 °C per minute ...
-
bioRxiv - Cancer Biology 2024Quote: ... and the assay was done on a Monolith instrument (NanoTemper Technologies).
-
bioRxiv - Biochemistry 2024Quote: Spectral shift assay was performed on a Monolith X instrument (NanoTemper Technologies) in parallel with MST measurements ...
-
bioRxiv - Neuroscience 2023Quote: ... Thermal shift assay was performed on Prometheus Panta NT.48 from NanoTemper technologies by measuring the intrinsic dual-UV fluorescence change in tryptophan and tyrosine residues in proteins at emission wavelengths of k = 330 and 350 nm.The ratio of the recorded emission intensities (Em350nm/Em330nm) ...
-
bioRxiv - Biochemistry 2023Quote: ... microscale thermophoresis (MST) assays were performed on a Monolith X (Nanotemper Technologies) using standard capillaries (MO-K022 ...
-
bioRxiv - Microbiology 2024Quote: The thermal shift assay was conducted using a Tycho NT.6 (NanoTemper) system ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: The MST assay was carried out on a Monolith NT.115 (NanoTemper Technologies). CK1γ2 was prepared at concentrations from 0.305 to 10000□nM and fluorescence dye from the Monolith His-tag Labeling kit RED-tris-NTA (NanoTemper Technologies ...
-
bioRxiv - Biochemistry 2024Quote: Thermal Unfolding Assays were conducted using a NanoTemper Panta using Prometheus Standard Capillaries (NanoTemper) (PR-C002) ...
-
bioRxiv - Biochemistry 2024Quote: Thermal Unfolding Assays were conducted using a NanoTemper Panta using Prometheus Standard Capillaries (NanoTemper) (PR-C002) ...
-
bioRxiv - Biochemistry 2020Quote: The microscale thermophoresis (MST) assay was performed using a Monolith NT.115 instrument (NanoTemper Technologies). For the MST assay ...
-
bioRxiv - Biophysics 2022Quote: EspB1-332 and free phospholipid or model membrane interaction assay was done using MST (Nanotemper)(43) ...
-
bioRxiv - Immunology 2023Quote: ... The final sample was validated using SDS-PAGE and thermostability assay (Nanotemper NT.48 nanoDSF).
-
bioRxiv - Developmental Biology 2023Quote: ... microscale thermophoresis (MST) assays were carried out by using a Monolish NT.115 (NanoTemper Technologies). His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper ...
-
bioRxiv - Biochemistry 2024Quote: Thermal stability assays were performed using a Prometheus NT.48 nanoDSF instrument (NanoTemper Inc., Germany). 50 μl of sample per condition were prepared to perform experiments in triplicates using standard-treated glass capillaries ...
-
bioRxiv - Plant Biology 2022Quote: ... cAMP binding assays to G-actin were performed with the Monolith NT.115 (Nanotemper, Munich, Germany) using 50 nM NTred-maleimid 2nd generation labelled G-actin and increasing concentrations of cAMP ...
-
bioRxiv - Cancer Biology 2023Quote: Label-free thermal shift assay experiments were performed with a Tycho NT.6 instrument (NanoTemper Technologies) based on a reported protocol 76 ...
-
bioRxiv - Biochemistry 2024Quote: Thermal shift assays were carried out in triplicate on the Prometheus NT.48 nanoDSF instrument (NanoTemper Technologies) using the manufacturer-designated capillaries ...
-
bioRxiv - Microbiology 2023Quote: ... thermal shift assays were performed with nanoDSF integrated in the Prometheus NT.48 (NanoTemper Technologies GmbH, Germany). Protein solutions of 75-100 µM of YopR WT ...
-
Defining short linear motif binding determinants by phage-based multiplexed deep mutational scanningbioRxiv - Biochemistry 2024Quote: ... Protein purity and quality was confirmed through SDS-PAGE and thermal shift assay (Tycho NT.6, NanoTemper). His-GST tagged domains of SARS-CoV-2 proteins were expressed and harvested as described above ...
-
bioRxiv - Biochemistry 2021Quote: Protein thermal stability was measured in a label-free fluorescence assay using a Prometheus NT.48 (NanoTemper, Germany). Briefly ...
-
bioRxiv - Biochemistry 2021Quote: MST assays were performed in a Monolith NT.115 using Standard Treated Glass Capillaries (NanoTemper Technologies MO-K002). His-TEV-UCH37(C88S)-RPN13C was labelled with Monolith His-Tag labeling Kit RED-tris-NTA (MO-L008 ...
-
bioRxiv - Cell Biology 2022Quote: ... employing the Monolith His-Tag Labeling Kit (RED-tris-NTA 2nd Generation kit, NanoTemper). The labeled protein was dissolved in a buffer containing PBS ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We used the Monolith Protein Labeling Kit RED-NHS 2nd Generation kit (NanoTemper MO- L011) to label ∼1 lysine per Fcy1 monomer with a fluorophore following the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2023Quote: Nucleotide binding assays based on microscale thermophoresis were performed with a Monolith NT.115 instrument (NanoTemper Technologies GmbH, Germany) using Monolith NT Premium Capillaries ...
-
bioRxiv - Microbiology 2024Quote: ... The MST assay was performed on a Monolith Instrument NT.115 device using standard treated capillaries (NanoTemper Technologies, Germany). The concentration of CsoR-GFP was constant at 250 nM ...
-
bioRxiv - Biophysics 2024Quote: ... The above-labeled concentrations were chosen based on fluorescence intensities from the pretest assay setup using MO.Control 1.5.3 software (NanoTemper Technologies GmbH). For this study ...
-
bioRxiv - Biochemistry 2020Quote: DSF assays were performed on LH3 wild-type and mutants using a Tycho NT.6 instrument (NanoTemper Technologies GmbH, Germany). LH3 samples at a concentration of 1 mg/mL in a buffer composed of 25 mM HEPES ...
-
bioRxiv - Cell Biology 2021Quote: Binding of cevipabulin to the seventh site was detected with an MST assay with a Monolith NT.115 instrument (NanoTemper Technologies). Purified tubulin was labeled using the Monolith protein labeling kit RED-NHS (NanoTemper Technologies ...
-
bioRxiv - Biophysics 2020Quote: The thermal stability of target proteins in different buffers and the potential stabilizing effects of ligands was evaluated at 1 μM using a direct thermal shift assay using a Tycho NT6 instrument (Nanotemper Technologies), monitoring intrinsic protein fluorescence at 330 and 350 nm during a thermal ramp from 35 °C to 95 °C ...
-
bioRxiv - Biophysics 2020Quote: ... The assay was performed in duplicate and the values reported were generated through the usage of MO Affinity Analysis software (NanoTemper Technologies).
-
bioRxiv - Molecular Biology 2020Quote: ... We measured the fluorescence signal from Gαi1β1γ1 by using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies). The samples were prepared in a buffer containing 20 mM sodium phosphate (pH 6.9) ...
-
bioRxiv - Microbiology 2023Quote: ... + 0.5% DDM and was labeled using a His-Tag Labeling Kit RED-tris-NTA 2nd Generation Kit (NanoTemper) via the manufacturers protocol in 1.7 ml low-adhesion tubes ...
-
bioRxiv - Biophysics 2021Quote: 6xHis-RAD51 was labelled with the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies), which specifically recognizes the hexahistidine tail of the recombinant protein.
-
bioRxiv - Cancer Biology 2020Quote: ... Microscale thermophoresis assays were performed on a NanoTemper ® Monolith NT.115 instrument with blue/red filters (NanoTemper Technologies GmbH, Munich, Germany). Samples were prepared in 20mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2020Quote: The binding of W25 and the structural integrity of Spike RBD was verified using a label-free thermal shift assay with Tycho NT.6 (Nanotemper Technologies GmbH) using intrinsic tryptophan and tyrosine fluorescence ...
-
bioRxiv - Biochemistry 2023Quote: In order to assess protein stability of all enzymes used in this study a thermal stability assay was performed using nanoDSF (Prometheus NT.48, NanoTemper Technologies GmbH). In this assay the enzymes were diluted to final concentrations of 0.5 mg/ml (USP1/UAF1 ...
-
bioRxiv - Neuroscience 2023Quote: ... Control experiments included measurements of the binding of the RED-tris-NTA dye to His-DJ-1 and of a dye-labeled AptamerCy5 to AMP using a manufacturer kit (Monolith NT.115 Control Kit RED, NanoTemper).
-
bioRxiv - Biochemistry 2024Quote: ... AcrVIB1-His was labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (Nanotemper, MO-L018) according to the manufacturer‘s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... and the NHS-red-tag kit (Nanotemper, Germany) was used to label ConA (Sigma ...